Differential behavior of non-albicans Candida species in the central nervous system of immunocompetent and immunosuppressed mice
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fmicb.2018.02968 http://hdl.handle.net/11449/190264 |
Resumo: | The genus Candida includes commensal fungi that can cause local and systemic infections, frequently involving vital organs as the central nervous system (CNS). Candida spp. occupy the fourth place among infections that affect the CNS. Although the incidence of Candida albicans is decreasing among patients under immunosuppressive therapies, the incidence of non-albicans Candida is increasing. In this context, the objective of this work was to evaluate the ability of non-albicans Candida species to spread to the CNS of immunocompetent and immunosuppressed mice. Adult female C57BL/6 mice were treated with prednisolone, intravenously infected with Candida glabrata, Candida krusei and Candida parapsilosis yeasts and then evaluated at the 3rd and 14th days after infection. All Candida species disseminated to the brain from immunocompetent animals and induced local inflammation at the third day post-infection. The immunosuppression resulted in body weight loss, leukopenia and reduced IL-2 production by spleen cell cultures. Higher fungal loads were recovered from the CNS of immunosuppressed mice. Inflammatory infiltration associated to a Th1 subset profile was higher in brain samples from C. krusei immunosuppressed mice compared with immunocompetent ones. Additionally, C. krusei was able to transform into pseudohypha inside microglia in vitro infected cells and also to induce elevated nitric oxide production. Altogether, these results indicate that C. glabrata, C. krusei and C. parapsilosis are able to disseminate to the CNS and promote local inflammation in both immunocompetent and immunosuppressed mice. C. krusei displayed a distinct behavior at the CNS triggering a local Th1 profile. The possible contribution of these non-albicans Candida species to other CNS pathologies as multiple sclerosis, Parkinson's and Alzheimer's diseases deserves further attention. |
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Differential behavior of non-albicans Candida species in the central nervous system of immunocompetent and immunosuppressed miceCandida spp.Fungal infectionsMicrogliaNeuroinflammationPrednisoloneThe genus Candida includes commensal fungi that can cause local and systemic infections, frequently involving vital organs as the central nervous system (CNS). Candida spp. occupy the fourth place among infections that affect the CNS. Although the incidence of Candida albicans is decreasing among patients under immunosuppressive therapies, the incidence of non-albicans Candida is increasing. In this context, the objective of this work was to evaluate the ability of non-albicans Candida species to spread to the CNS of immunocompetent and immunosuppressed mice. Adult female C57BL/6 mice were treated with prednisolone, intravenously infected with Candida glabrata, Candida krusei and Candida parapsilosis yeasts and then evaluated at the 3rd and 14th days after infection. All Candida species disseminated to the brain from immunocompetent animals and induced local inflammation at the third day post-infection. The immunosuppression resulted in body weight loss, leukopenia and reduced IL-2 production by spleen cell cultures. Higher fungal loads were recovered from the CNS of immunosuppressed mice. Inflammatory infiltration associated to a Th1 subset profile was higher in brain samples from C. krusei immunosuppressed mice compared with immunocompetent ones. Additionally, C. krusei was able to transform into pseudohypha inside microglia in vitro infected cells and also to induce elevated nitric oxide production. Altogether, these results indicate that C. glabrata, C. krusei and C. parapsilosis are able to disseminate to the CNS and promote local inflammation in both immunocompetent and immunosuppressed mice. C. krusei displayed a distinct behavior at the CNS triggering a local Th1 profile. The possible contribution of these non-albicans Candida species to other CNS pathologies as multiple sclerosis, Parkinson's and Alzheimer's diseases deserves further attention.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Botucatu Medical School São Paulo State University (UNESP)Institute of Biosciences São Paulo State University (UNESP)Botucatu Medical School São Paulo State University (UNESP)Institute of Biosciences São Paulo State University (UNESP)CNPq: 152790/2016-0CNPq: 307269/2017-5Universidade Estadual Paulista (Unesp)Sanches, Marcelo D'Alessandre [UNESP]Mimura, Luiza A.N. [UNESP]Oliveira, Larissa R.C. [UNESP]Ishikawa, Larissa L.W. [UNESP]Garces, Hans G. [UNESP]Bagagli, Eduardo [UNESP]Sartori, Alexandrina [UNESP]Kurokawa, Cilmery Suemi [UNESP]Fraga-Silva, Thais F.C. [UNESP]2019-10-06T17:07:34Z2019-10-06T17:07:34Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fmicb.2018.02968Frontiers in Microbiology, v. 10, n. JAN, 2019.1664-302Xhttp://hdl.handle.net/11449/19026410.3389/fmicb.2018.029682-s2.0-85064415950332032757042953985104232695404650000-0002-8003-41090000-0003-1380-7527Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Microbiologyinfo:eu-repo/semantics/openAccess2024-09-03T13:46:38Zoai:repositorio.unesp.br:11449/190264Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:46:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Differential behavior of non-albicans Candida species in the central nervous system of immunocompetent and immunosuppressed mice |
title |
Differential behavior of non-albicans Candida species in the central nervous system of immunocompetent and immunosuppressed mice |
spellingShingle |
Differential behavior of non-albicans Candida species in the central nervous system of immunocompetent and immunosuppressed mice Sanches, Marcelo D'Alessandre [UNESP] Candida spp. Fungal infections Microglia Neuroinflammation Prednisolone |
title_short |
Differential behavior of non-albicans Candida species in the central nervous system of immunocompetent and immunosuppressed mice |
title_full |
Differential behavior of non-albicans Candida species in the central nervous system of immunocompetent and immunosuppressed mice |
title_fullStr |
Differential behavior of non-albicans Candida species in the central nervous system of immunocompetent and immunosuppressed mice |
title_full_unstemmed |
Differential behavior of non-albicans Candida species in the central nervous system of immunocompetent and immunosuppressed mice |
title_sort |
Differential behavior of non-albicans Candida species in the central nervous system of immunocompetent and immunosuppressed mice |
author |
Sanches, Marcelo D'Alessandre [UNESP] |
author_facet |
Sanches, Marcelo D'Alessandre [UNESP] Mimura, Luiza A.N. [UNESP] Oliveira, Larissa R.C. [UNESP] Ishikawa, Larissa L.W. [UNESP] Garces, Hans G. [UNESP] Bagagli, Eduardo [UNESP] Sartori, Alexandrina [UNESP] Kurokawa, Cilmery Suemi [UNESP] Fraga-Silva, Thais F.C. [UNESP] |
author_role |
author |
author2 |
Mimura, Luiza A.N. [UNESP] Oliveira, Larissa R.C. [UNESP] Ishikawa, Larissa L.W. [UNESP] Garces, Hans G. [UNESP] Bagagli, Eduardo [UNESP] Sartori, Alexandrina [UNESP] Kurokawa, Cilmery Suemi [UNESP] Fraga-Silva, Thais F.C. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Sanches, Marcelo D'Alessandre [UNESP] Mimura, Luiza A.N. [UNESP] Oliveira, Larissa R.C. [UNESP] Ishikawa, Larissa L.W. [UNESP] Garces, Hans G. [UNESP] Bagagli, Eduardo [UNESP] Sartori, Alexandrina [UNESP] Kurokawa, Cilmery Suemi [UNESP] Fraga-Silva, Thais F.C. [UNESP] |
dc.subject.por.fl_str_mv |
Candida spp. Fungal infections Microglia Neuroinflammation Prednisolone |
topic |
Candida spp. Fungal infections Microglia Neuroinflammation Prednisolone |
description |
The genus Candida includes commensal fungi that can cause local and systemic infections, frequently involving vital organs as the central nervous system (CNS). Candida spp. occupy the fourth place among infections that affect the CNS. Although the incidence of Candida albicans is decreasing among patients under immunosuppressive therapies, the incidence of non-albicans Candida is increasing. In this context, the objective of this work was to evaluate the ability of non-albicans Candida species to spread to the CNS of immunocompetent and immunosuppressed mice. Adult female C57BL/6 mice were treated with prednisolone, intravenously infected with Candida glabrata, Candida krusei and Candida parapsilosis yeasts and then evaluated at the 3rd and 14th days after infection. All Candida species disseminated to the brain from immunocompetent animals and induced local inflammation at the third day post-infection. The immunosuppression resulted in body weight loss, leukopenia and reduced IL-2 production by spleen cell cultures. Higher fungal loads were recovered from the CNS of immunosuppressed mice. Inflammatory infiltration associated to a Th1 subset profile was higher in brain samples from C. krusei immunosuppressed mice compared with immunocompetent ones. Additionally, C. krusei was able to transform into pseudohypha inside microglia in vitro infected cells and also to induce elevated nitric oxide production. Altogether, these results indicate that C. glabrata, C. krusei and C. parapsilosis are able to disseminate to the CNS and promote local inflammation in both immunocompetent and immunosuppressed mice. C. krusei displayed a distinct behavior at the CNS triggering a local Th1 profile. The possible contribution of these non-albicans Candida species to other CNS pathologies as multiple sclerosis, Parkinson's and Alzheimer's diseases deserves further attention. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T17:07:34Z 2019-10-06T17:07:34Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fmicb.2018.02968 Frontiers in Microbiology, v. 10, n. JAN, 2019. 1664-302X http://hdl.handle.net/11449/190264 10.3389/fmicb.2018.02968 2-s2.0-85064415950 3320327570429539 8510423269540465 0000-0002-8003-4109 0000-0003-1380-7527 |
url |
http://dx.doi.org/10.3389/fmicb.2018.02968 http://hdl.handle.net/11449/190264 |
identifier_str_mv |
Frontiers in Microbiology, v. 10, n. JAN, 2019. 1664-302X 10.3389/fmicb.2018.02968 2-s2.0-85064415950 3320327570429539 8510423269540465 0000-0002-8003-4109 0000-0003-1380-7527 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021389968605184 |