Preliminary Survey and Study of the CymMV Variability in Orchids of Sao Paulo State, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/161142 |
Resumo: | Viruses are a major problem in orchid production systems, as they promote considerable damage to its commercial value, since they directly affect its aesthetic appeal as well as productivity. The Potexvirus Cymbidium mosaic virus (CymMV) and the Tobamovirus Odontoglossum ringspot virus (ORSV) are of great economic importance worldwide. A survey of the viruses present in 232 orchid samples belonging to different genera and collected from 12 Sao Paulo State cities was carried out. The diagnosis was made by direct ELISA test for the detection of CymMV, ORSV, TSWV (Tomato spotted wilt virus), and CymRSV (Cymbidium ringspot virus), as well as indirect ELISA for Potyvirus detection, using universal antiserum against this genus. The results were confirmed by RT-PCR, with specific oligonucleotides designed for the coat protein (CP) gene of the CymMV and ORSV, as well as for the detection of Orchid fleck virus (OFV). Of the total of samples tested, 153 were positive for CymMV (65.9%), and 40 were positive for ORSV (17.2%). CymRSV, TSWV, OFV or any Potyvirus were not found in the samples analyzed. In 90% of the samples in which ORSV was detected, CymMV was also present in mixed infections. Nucleotide identity of the CymMV sequenced isolates ranged between 91 and 92% with CymMV genbank accession numbers AY571289.1, AB541572.1 and AB197937.1, and the phylogenetic tree supposes that, so far, this virus has shown a low variability rate, in terms of CP gene. These results denote the CymMV importance in the orchid production systems in Sao Paulo State, as well as possible synergistic link between this virus and ORSV. |
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Preliminary Survey and Study of the CymMV Variability in Orchids of Sao Paulo State, BrazildetectionsymptomsOrchidaceaeCymbidium mosaic virusOdontoglossum ringspot virusViruses are a major problem in orchid production systems, as they promote considerable damage to its commercial value, since they directly affect its aesthetic appeal as well as productivity. The Potexvirus Cymbidium mosaic virus (CymMV) and the Tobamovirus Odontoglossum ringspot virus (ORSV) are of great economic importance worldwide. A survey of the viruses present in 232 orchid samples belonging to different genera and collected from 12 Sao Paulo State cities was carried out. The diagnosis was made by direct ELISA test for the detection of CymMV, ORSV, TSWV (Tomato spotted wilt virus), and CymRSV (Cymbidium ringspot virus), as well as indirect ELISA for Potyvirus detection, using universal antiserum against this genus. The results were confirmed by RT-PCR, with specific oligonucleotides designed for the coat protein (CP) gene of the CymMV and ORSV, as well as for the detection of Orchid fleck virus (OFV). Of the total of samples tested, 153 were positive for CymMV (65.9%), and 40 were positive for ORSV (17.2%). CymRSV, TSWV, OFV or any Potyvirus were not found in the samples analyzed. In 90% of the samples in which ORSV was detected, CymMV was also present in mixed infections. Nucleotide identity of the CymMV sequenced isolates ranged between 91 and 92% with CymMV genbank accession numbers AY571289.1, AB541572.1 and AB197937.1, and the phylogenetic tree supposes that, so far, this virus has shown a low variability rate, in terms of CP gene. These results denote the CymMV importance in the orchid production systems in Sao Paulo State, as well as possible synergistic link between this virus and ORSV.Sao Paulo State Univ, Coll Agron Sci, Dept Vegetal Prod, Botucatu, SP, BrazilSao Paulo State Univ, Coll Agron Sci, Dept Vegetal Prod, Botucatu, SP, BrazilInt Soc Horticultural ScienceUniversidade Estadual Paulista (Unesp)Moraes, L. A. [UNESP]Pavan, M. A. [UNESP]Sakate, R. K. [UNESP]Blystad, D. R.Spetz, C.Floistad, E.2018-11-26T16:19:18Z2018-11-26T16:19:18Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article55-61Xiii International Symposium On Virus Diseases Of Ornamental Plants. Leuven 1: Int Soc Horticultural Science, v. 1072, p. 55-61, 2015.0567-7572http://hdl.handle.net/11449/161142WOS:00036841630000596598228556976850000-0001-7526-640XWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengXiii International Symposium On Virus Diseases Of Ornamental Plants0,198info:eu-repo/semantics/openAccess2024-04-30T15:56:15Zoai:repositorio.unesp.br:11449/161142Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:02:55.640933Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Preliminary Survey and Study of the CymMV Variability in Orchids of Sao Paulo State, Brazil |
title |
Preliminary Survey and Study of the CymMV Variability in Orchids of Sao Paulo State, Brazil |
spellingShingle |
Preliminary Survey and Study of the CymMV Variability in Orchids of Sao Paulo State, Brazil Moraes, L. A. [UNESP] detection symptoms Orchidaceae Cymbidium mosaic virus Odontoglossum ringspot virus |
title_short |
Preliminary Survey and Study of the CymMV Variability in Orchids of Sao Paulo State, Brazil |
title_full |
Preliminary Survey and Study of the CymMV Variability in Orchids of Sao Paulo State, Brazil |
title_fullStr |
Preliminary Survey and Study of the CymMV Variability in Orchids of Sao Paulo State, Brazil |
title_full_unstemmed |
Preliminary Survey and Study of the CymMV Variability in Orchids of Sao Paulo State, Brazil |
title_sort |
Preliminary Survey and Study of the CymMV Variability in Orchids of Sao Paulo State, Brazil |
author |
Moraes, L. A. [UNESP] |
author_facet |
Moraes, L. A. [UNESP] Pavan, M. A. [UNESP] Sakate, R. K. [UNESP] Blystad, D. R. Spetz, C. Floistad, E. |
author_role |
author |
author2 |
Pavan, M. A. [UNESP] Sakate, R. K. [UNESP] Blystad, D. R. Spetz, C. Floistad, E. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Moraes, L. A. [UNESP] Pavan, M. A. [UNESP] Sakate, R. K. [UNESP] Blystad, D. R. Spetz, C. Floistad, E. |
dc.subject.por.fl_str_mv |
detection symptoms Orchidaceae Cymbidium mosaic virus Odontoglossum ringspot virus |
topic |
detection symptoms Orchidaceae Cymbidium mosaic virus Odontoglossum ringspot virus |
description |
Viruses are a major problem in orchid production systems, as they promote considerable damage to its commercial value, since they directly affect its aesthetic appeal as well as productivity. The Potexvirus Cymbidium mosaic virus (CymMV) and the Tobamovirus Odontoglossum ringspot virus (ORSV) are of great economic importance worldwide. A survey of the viruses present in 232 orchid samples belonging to different genera and collected from 12 Sao Paulo State cities was carried out. The diagnosis was made by direct ELISA test for the detection of CymMV, ORSV, TSWV (Tomato spotted wilt virus), and CymRSV (Cymbidium ringspot virus), as well as indirect ELISA for Potyvirus detection, using universal antiserum against this genus. The results were confirmed by RT-PCR, with specific oligonucleotides designed for the coat protein (CP) gene of the CymMV and ORSV, as well as for the detection of Orchid fleck virus (OFV). Of the total of samples tested, 153 were positive for CymMV (65.9%), and 40 were positive for ORSV (17.2%). CymRSV, TSWV, OFV or any Potyvirus were not found in the samples analyzed. In 90% of the samples in which ORSV was detected, CymMV was also present in mixed infections. Nucleotide identity of the CymMV sequenced isolates ranged between 91 and 92% with CymMV genbank accession numbers AY571289.1, AB541572.1 and AB197937.1, and the phylogenetic tree supposes that, so far, this virus has shown a low variability rate, in terms of CP gene. These results denote the CymMV importance in the orchid production systems in Sao Paulo State, as well as possible synergistic link between this virus and ORSV. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01 2018-11-26T16:19:18Z 2018-11-26T16:19:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Xiii International Symposium On Virus Diseases Of Ornamental Plants. Leuven 1: Int Soc Horticultural Science, v. 1072, p. 55-61, 2015. 0567-7572 http://hdl.handle.net/11449/161142 WOS:000368416300005 9659822855697685 0000-0001-7526-640X |
identifier_str_mv |
Xiii International Symposium On Virus Diseases Of Ornamental Plants. Leuven 1: Int Soc Horticultural Science, v. 1072, p. 55-61, 2015. 0567-7572 WOS:000368416300005 9659822855697685 0000-0001-7526-640X |
url |
http://hdl.handle.net/11449/161142 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Xiii International Symposium On Virus Diseases Of Ornamental Plants 0,198 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
55-61 |
dc.publisher.none.fl_str_mv |
Int Soc Horticultural Science |
publisher.none.fl_str_mv |
Int Soc Horticultural Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128745710551040 |