Production of biosurfactant by Lysinibacillus sp. and Bacillus sp. from different oils as carbon source
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1517-707620200004.1149 http://hdl.handle.net/11449/209005 |
Resumo: | Biosurfactants are multifunctional molecules produced by microorganisms and have some advantages compared to synthetic surfactants, such as low toxicity, high biodegradability ,improved tensoactivity and chemical diversity. However, commercial scale production is not viable due to the high cost of the substrates used. Therefore, in the present work we evaluated the production of biosurfactants using different oils as carbon source, such as soybean oil, frying oil, diesel oil, new and used lubricating oil, from two genera of bacteria isolated from sludge cosmetics industry and soil contaminated with diesel oil, Lysinibacillus sp. and Bacillus sp. respectively. These were identified by sequencing the 16S rRNA gene fragment and kept under refrigeration at 4 degrees C in test tubes with tryptone soy agar. The production was carried out under agitation at 200 rpm at 30 degrees C for 7 days. The biosurfactant production was analyzed by emulsifying activity, emulsification index and drop collapse. The bacteria used produced biosurfactant in all types of oils tested. Soybean oil was the best substrate for the production of biosurfactant from Lysinibacillus sp. and Bacillus sp. with an emulsification index of 50% and 46.63%, respectively. |
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Production of biosurfactant by Lysinibacillus sp. and Bacillus sp. from different oils as carbon sourceEmulsifiersbacteriumalternative sourcesfermentationBiosurfactants are multifunctional molecules produced by microorganisms and have some advantages compared to synthetic surfactants, such as low toxicity, high biodegradability ,improved tensoactivity and chemical diversity. However, commercial scale production is not viable due to the high cost of the substrates used. Therefore, in the present work we evaluated the production of biosurfactants using different oils as carbon source, such as soybean oil, frying oil, diesel oil, new and used lubricating oil, from two genera of bacteria isolated from sludge cosmetics industry and soil contaminated with diesel oil, Lysinibacillus sp. and Bacillus sp. respectively. These were identified by sequencing the 16S rRNA gene fragment and kept under refrigeration at 4 degrees C in test tubes with tryptone soy agar. The production was carried out under agitation at 200 rpm at 30 degrees C for 7 days. The biosurfactant production was analyzed by emulsifying activity, emulsification index and drop collapse. The bacteria used produced biosurfactant in all types of oils tested. Soybean oil was the best substrate for the production of biosurfactant from Lysinibacillus sp. and Bacillus sp. with an emulsification index of 50% and 46.63%, respectively.Univ Fed Sao Carlos, Dept Biol, Campus Sorocaba,Rodovia Joao Leme Santos,SP 264, BR-18052780 Sorocaba, SP, BrazilUniv Estadual Paulista, Campus Sorocaba,Ave Tres Marco 511, Sorocaba, SP, BrazilUniv Estadual Paulista, Campus Sorocaba,Ave Tres Marco 511, Sorocaba, SP, BrazilUniv Fed Rio De Janeiro, Lab HidrogenioUniversidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (Unesp)Mendes de Almeida, Grazieny AparecidaAraujo Vieira Neta, Maria dos RemediosSilva, Gabriela Fiori daPrado, Pierre Ferreira do [UNESP]Almeida, Monica Aparecida deSilveira Duarte, Iolanda Cristina2021-06-25T11:45:42Z2021-06-25T11:45:42Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://dx.doi.org/10.1590/S1517-707620200004.1149Materia-rio De Janeiro. Rio De Janeiro Rj: Univ Fed Rio De Janeiro, Lab Hidrogenio, v. 25, n. 4, 10 p., 2020.1517-7076http://hdl.handle.net/11449/20900510.1590/S1517-707620200004.1149S1517-70762020000400309WOS:000598512700010S1517-70762020000400309.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporMateria-rio De Janeiroinfo:eu-repo/semantics/openAccess2024-01-13T06:30:39Zoai:repositorio.unesp.br:11449/209005Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:49:07.226934Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Production of biosurfactant by Lysinibacillus sp. and Bacillus sp. from different oils as carbon source |
title |
Production of biosurfactant by Lysinibacillus sp. and Bacillus sp. from different oils as carbon source |
spellingShingle |
Production of biosurfactant by Lysinibacillus sp. and Bacillus sp. from different oils as carbon source Mendes de Almeida, Grazieny Aparecida Emulsifiers bacterium alternative sources fermentation |
title_short |
Production of biosurfactant by Lysinibacillus sp. and Bacillus sp. from different oils as carbon source |
title_full |
Production of biosurfactant by Lysinibacillus sp. and Bacillus sp. from different oils as carbon source |
title_fullStr |
Production of biosurfactant by Lysinibacillus sp. and Bacillus sp. from different oils as carbon source |
title_full_unstemmed |
Production of biosurfactant by Lysinibacillus sp. and Bacillus sp. from different oils as carbon source |
title_sort |
Production of biosurfactant by Lysinibacillus sp. and Bacillus sp. from different oils as carbon source |
author |
Mendes de Almeida, Grazieny Aparecida |
author_facet |
Mendes de Almeida, Grazieny Aparecida Araujo Vieira Neta, Maria dos Remedios Silva, Gabriela Fiori da Prado, Pierre Ferreira do [UNESP] Almeida, Monica Aparecida de Silveira Duarte, Iolanda Cristina |
author_role |
author |
author2 |
Araujo Vieira Neta, Maria dos Remedios Silva, Gabriela Fiori da Prado, Pierre Ferreira do [UNESP] Almeida, Monica Aparecida de Silveira Duarte, Iolanda Cristina |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Carlos (UFSCar) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Mendes de Almeida, Grazieny Aparecida Araujo Vieira Neta, Maria dos Remedios Silva, Gabriela Fiori da Prado, Pierre Ferreira do [UNESP] Almeida, Monica Aparecida de Silveira Duarte, Iolanda Cristina |
dc.subject.por.fl_str_mv |
Emulsifiers bacterium alternative sources fermentation |
topic |
Emulsifiers bacterium alternative sources fermentation |
description |
Biosurfactants are multifunctional molecules produced by microorganisms and have some advantages compared to synthetic surfactants, such as low toxicity, high biodegradability ,improved tensoactivity and chemical diversity. However, commercial scale production is not viable due to the high cost of the substrates used. Therefore, in the present work we evaluated the production of biosurfactants using different oils as carbon source, such as soybean oil, frying oil, diesel oil, new and used lubricating oil, from two genera of bacteria isolated from sludge cosmetics industry and soil contaminated with diesel oil, Lysinibacillus sp. and Bacillus sp. respectively. These were identified by sequencing the 16S rRNA gene fragment and kept under refrigeration at 4 degrees C in test tubes with tryptone soy agar. The production was carried out under agitation at 200 rpm at 30 degrees C for 7 days. The biosurfactant production was analyzed by emulsifying activity, emulsification index and drop collapse. The bacteria used produced biosurfactant in all types of oils tested. Soybean oil was the best substrate for the production of biosurfactant from Lysinibacillus sp. and Bacillus sp. with an emulsification index of 50% and 46.63%, respectively. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 2021-06-25T11:45:42Z 2021-06-25T11:45:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1517-707620200004.1149 Materia-rio De Janeiro. Rio De Janeiro Rj: Univ Fed Rio De Janeiro, Lab Hidrogenio, v. 25, n. 4, 10 p., 2020. 1517-7076 http://hdl.handle.net/11449/209005 10.1590/S1517-707620200004.1149 S1517-70762020000400309 WOS:000598512700010 S1517-70762020000400309.pdf |
url |
http://dx.doi.org/10.1590/S1517-707620200004.1149 http://hdl.handle.net/11449/209005 |
identifier_str_mv |
Materia-rio De Janeiro. Rio De Janeiro Rj: Univ Fed Rio De Janeiro, Lab Hidrogenio, v. 25, n. 4, 10 p., 2020. 1517-7076 10.1590/S1517-707620200004.1149 S1517-70762020000400309 WOS:000598512700010 S1517-70762020000400309.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Materia-rio De Janeiro |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 application/pdf |
dc.publisher.none.fl_str_mv |
Univ Fed Rio De Janeiro, Lab Hidrogenio |
publisher.none.fl_str_mv |
Univ Fed Rio De Janeiro, Lab Hidrogenio |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129465643958272 |