In vitro synergistic effects of fluoxetine and paroxetine in combination with amphotericin B against Cryptococcus neoformans
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/femspd/ftab001 http://hdl.handle.net/11449/207415 |
Resumo: | Cryptococcus neoformans is a yeast that mainly affects immunocompromised individuals and causes meningoencephalitis depending on the immune status of the host. The present study aimed to validate the efficacy of selective serotonin reuptake inhibitors, fluoxetine hydrochloride (FLH) and paroxetine hydrochloride (PAH), alone and in combination with amphotericin B (AmB) against C. neoformans. Susceptibility tests were conducted using the broth microdilution method and synergistic effects of combining FLH and PAH with AmB were analyzed using the checkerboard assay. Effects of minimum inhibitory concentration (MIC) and synergistic concentration were evaluated in biofilms by quantifying the biomass, measuring the viability by counting the colony-forming units (CFU/mL) and examining the size of the induced capsules. Cryptococcus neoformans was susceptible to FLH and PAH and the synergistic effect of FLH and PAH in combination with AmB reduced the MIC of AmB by up to 8-fold. The isolated substances and combination with AmB were able to reduce biofilm biomass and biofilm viability. In addition, FLH and PAH alone or in combination with AmB significantly decreased the size of the yeast capsules. Collectively, our results indicate the use of FLH and PAH as a promising prototype for the development of anti-cryptococcal drugs. |
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In vitro synergistic effects of fluoxetine and paroxetine in combination with amphotericin B against Cryptococcus neoformansAntifungalCryptococcus neoformansFluoxetine hydrochlorideParoxetine hydrochlorideSelective serotonin reuptake inhibitorsSynergismCryptococcus neoformans is a yeast that mainly affects immunocompromised individuals and causes meningoencephalitis depending on the immune status of the host. The present study aimed to validate the efficacy of selective serotonin reuptake inhibitors, fluoxetine hydrochloride (FLH) and paroxetine hydrochloride (PAH), alone and in combination with amphotericin B (AmB) against C. neoformans. Susceptibility tests were conducted using the broth microdilution method and synergistic effects of combining FLH and PAH with AmB were analyzed using the checkerboard assay. Effects of minimum inhibitory concentration (MIC) and synergistic concentration were evaluated in biofilms by quantifying the biomass, measuring the viability by counting the colony-forming units (CFU/mL) and examining the size of the induced capsules. Cryptococcus neoformans was susceptible to FLH and PAH and the synergistic effect of FLH and PAH in combination with AmB reduced the MIC of AmB by up to 8-fold. The isolated substances and combination with AmB were able to reduce biofilm biomass and biofilm viability. In addition, FLH and PAH alone or in combination with AmB significantly decreased the size of the yeast capsules. Collectively, our results indicate the use of FLH and PAH as a promising prototype for the development of anti-cryptococcal drugs.Department of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP), 777 São José dos CamposInstituto Carlos Chagas Fundação Oswaldo Cruz (Fiocruz) Rua Prof. Algacyr Munhoz MaderDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP), 777 São José dos CamposUniversidade Estadual Paulista (Unesp)Rua Prof. Algacyr Munhoz MaderPereira, Thaís Cristine [UNESP]De Menezes, Raquel Teles [UNESP]De Oliveira, Haroldo CesarDe Oliveira, Luciane Dias [UNESP]Scorzoni, Liliana [UNESP]2021-06-25T10:54:46Z2021-06-25T10:54:46Z2021-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1093/femspd/ftab001Pathogens and Disease, v. 79, n. 2, 2021.2049-632Xhttp://hdl.handle.net/11449/20741510.1093/femspd/ftab0012-s2.0-85102214269Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPathogens and Diseaseinfo:eu-repo/semantics/openAccess2021-10-23T17:09:29Zoai:repositorio.unesp.br:11449/207415Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T17:09:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In vitro synergistic effects of fluoxetine and paroxetine in combination with amphotericin B against Cryptococcus neoformans |
title |
In vitro synergistic effects of fluoxetine and paroxetine in combination with amphotericin B against Cryptococcus neoformans |
spellingShingle |
In vitro synergistic effects of fluoxetine and paroxetine in combination with amphotericin B against Cryptococcus neoformans Pereira, Thaís Cristine [UNESP] Antifungal Cryptococcus neoformans Fluoxetine hydrochloride Paroxetine hydrochloride Selective serotonin reuptake inhibitors Synergism |
title_short |
In vitro synergistic effects of fluoxetine and paroxetine in combination with amphotericin B against Cryptococcus neoformans |
title_full |
In vitro synergistic effects of fluoxetine and paroxetine in combination with amphotericin B against Cryptococcus neoformans |
title_fullStr |
In vitro synergistic effects of fluoxetine and paroxetine in combination with amphotericin B against Cryptococcus neoformans |
title_full_unstemmed |
In vitro synergistic effects of fluoxetine and paroxetine in combination with amphotericin B against Cryptococcus neoformans |
title_sort |
In vitro synergistic effects of fluoxetine and paroxetine in combination with amphotericin B against Cryptococcus neoformans |
author |
Pereira, Thaís Cristine [UNESP] |
author_facet |
Pereira, Thaís Cristine [UNESP] De Menezes, Raquel Teles [UNESP] De Oliveira, Haroldo Cesar De Oliveira, Luciane Dias [UNESP] Scorzoni, Liliana [UNESP] |
author_role |
author |
author2 |
De Menezes, Raquel Teles [UNESP] De Oliveira, Haroldo Cesar De Oliveira, Luciane Dias [UNESP] Scorzoni, Liliana [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Rua Prof. Algacyr Munhoz Mader |
dc.contributor.author.fl_str_mv |
Pereira, Thaís Cristine [UNESP] De Menezes, Raquel Teles [UNESP] De Oliveira, Haroldo Cesar De Oliveira, Luciane Dias [UNESP] Scorzoni, Liliana [UNESP] |
dc.subject.por.fl_str_mv |
Antifungal Cryptococcus neoformans Fluoxetine hydrochloride Paroxetine hydrochloride Selective serotonin reuptake inhibitors Synergism |
topic |
Antifungal Cryptococcus neoformans Fluoxetine hydrochloride Paroxetine hydrochloride Selective serotonin reuptake inhibitors Synergism |
description |
Cryptococcus neoformans is a yeast that mainly affects immunocompromised individuals and causes meningoencephalitis depending on the immune status of the host. The present study aimed to validate the efficacy of selective serotonin reuptake inhibitors, fluoxetine hydrochloride (FLH) and paroxetine hydrochloride (PAH), alone and in combination with amphotericin B (AmB) against C. neoformans. Susceptibility tests were conducted using the broth microdilution method and synergistic effects of combining FLH and PAH with AmB were analyzed using the checkerboard assay. Effects of minimum inhibitory concentration (MIC) and synergistic concentration were evaluated in biofilms by quantifying the biomass, measuring the viability by counting the colony-forming units (CFU/mL) and examining the size of the induced capsules. Cryptococcus neoformans was susceptible to FLH and PAH and the synergistic effect of FLH and PAH in combination with AmB reduced the MIC of AmB by up to 8-fold. The isolated substances and combination with AmB were able to reduce biofilm biomass and biofilm viability. In addition, FLH and PAH alone or in combination with AmB significantly decreased the size of the yeast capsules. Collectively, our results indicate the use of FLH and PAH as a promising prototype for the development of anti-cryptococcal drugs. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:54:46Z 2021-06-25T10:54:46Z 2021-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/femspd/ftab001 Pathogens and Disease, v. 79, n. 2, 2021. 2049-632X http://hdl.handle.net/11449/207415 10.1093/femspd/ftab001 2-s2.0-85102214269 |
url |
http://dx.doi.org/10.1093/femspd/ftab001 http://hdl.handle.net/11449/207415 |
identifier_str_mv |
Pathogens and Disease, v. 79, n. 2, 2021. 2049-632X 10.1093/femspd/ftab001 2-s2.0-85102214269 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pathogens and Disease |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964956984082432 |