Performance of selective and differential media in the primary isolation of yeasts from different biological samples

Detalhes bibliográficos
Autor(a) principal: Silva, Jaqueline Otero
Data de Publicação: 2004
Outros Autores: Franceschini, Silvio Antônio, Lavrador, Marco Aurélio Sicchiroli, Candido, Regina Célia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1023/B:MYCO.0000012223.38967.7d
http://hdl.handle.net/11449/230970
Resumo: In view of the increase in yeast infections, especially polymicrobial ones, differential culture media have acquired increasing importance. The present study evaluated the Sabouraud chloramphenicol, Biggy agar, Pagano Levin agar and CHROMagar Candida media in terms of isolation, number of yeast colony forming units per plate, and inhibition of bacteria and filamentous fungi. To this end, we used 223 biological samples, including feces, and oral, vaginal and anal mucosae from 86 patients presenting or not symptoms of fungal infections. The four media did not differ significantly in terms of detection of yeast-positive cultures. The number of colony forming units per plate ranged from zero to 2.380, with a predominance of counts of 1 to 9 colonies per plate. No significant differences were observed among the four culture media in terms of number of colonies counted, for each kind of biological material. Fifteen species belonging to the genera Candida, Saccharomyces, Cryptococcus, Trichosporon and Rhodotorula were isolated, with C. albicans being the predominant species, followed by C. parapsilosis and R. rubra. CHROMagar Candida and Biggy agar were complementary in the isolation of the different species and favored a greater recovery of polymicrobial cultures. Pagano Levin agar isolated the smallest variety of species. Sabouraud chloramphenicol agar was the least effective in terms of bacterial inhibition and favored a greater development of filamentous fungi. The results suggest that more than one culture medium should be used for an adequate primary isolation.
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spelling Performance of selective and differential media in the primary isolation of yeasts from different biological samplesBiggy agarCandidaCHROMagar CandidaCulture mediaPagano Levin agarYeastsIn view of the increase in yeast infections, especially polymicrobial ones, differential culture media have acquired increasing importance. The present study evaluated the Sabouraud chloramphenicol, Biggy agar, Pagano Levin agar and CHROMagar Candida media in terms of isolation, number of yeast colony forming units per plate, and inhibition of bacteria and filamentous fungi. To this end, we used 223 biological samples, including feces, and oral, vaginal and anal mucosae from 86 patients presenting or not symptoms of fungal infections. The four media did not differ significantly in terms of detection of yeast-positive cultures. The number of colony forming units per plate ranged from zero to 2.380, with a predominance of counts of 1 to 9 colonies per plate. No significant differences were observed among the four culture media in terms of number of colonies counted, for each kind of biological material. Fifteen species belonging to the genera Candida, Saccharomyces, Cryptococcus, Trichosporon and Rhodotorula were isolated, with C. albicans being the predominant species, followed by C. parapsilosis and R. rubra. CHROMagar Candida and Biggy agar were complementary in the isolation of the different species and favored a greater recovery of polymicrobial cultures. Pagano Levin agar isolated the smallest variety of species. Sabouraud chloramphenicol agar was the least effective in terms of bacterial inhibition and favored a greater development of filamentous fungi. The results suggest that more than one culture medium should be used for an adequate primary isolation.Instituto Adolfo Lutz Lab. I de Ribeirão Preto, São PauloDepartamento de Microbiologia Faculdade Cie. Agrarias V. Univ. do Estado de São Paulo, SPSistema Integrado de Saúde Universidade de São Paulo, Ribeirão Preto, SPDepto. de Fís. e Quím. Faculdade Cie. Farmaceuticas R. Universidade de São Paulo, SPDepto. Analises Clinicas, T. Faculdade Cie. Farmaceuticas R. Universidade de São Paulo, SPInstitute Adolfo Lutz Lab. I de Ribeirão Preto, Rua Minas, 877, CEP 14 085-410, Ribeirao Preto, SPLab. I de Ribeirão PretoUniv. do Estado de São PauloUniversidade de São Paulo (USP)Silva, Jaqueline OteroFranceschini, Silvio AntônioLavrador, Marco Aurélio SicchiroliCandido, Regina Célia2022-04-29T08:42:55Z2022-04-29T08:42:55Z2004-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article29-36http://dx.doi.org/10.1023/B:MYCO.0000012223.38967.7dMycopathologia, v. 157, n. 1, p. 29-36, 2004.0301-486Xhttp://hdl.handle.net/11449/23097010.1023/B:MYCO.0000012223.38967.7d2-s2.0-1242310597Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMycopathologiainfo:eu-repo/semantics/openAccess2024-06-21T15:18:33Zoai:repositorio.unesp.br:11449/230970Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:01:56.573366Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Performance of selective and differential media in the primary isolation of yeasts from different biological samples
title Performance of selective and differential media in the primary isolation of yeasts from different biological samples
spellingShingle Performance of selective and differential media in the primary isolation of yeasts from different biological samples
Silva, Jaqueline Otero
Biggy agar
Candida
CHROMagar Candida
Culture media
Pagano Levin agar
Yeasts
title_short Performance of selective and differential media in the primary isolation of yeasts from different biological samples
title_full Performance of selective and differential media in the primary isolation of yeasts from different biological samples
title_fullStr Performance of selective and differential media in the primary isolation of yeasts from different biological samples
title_full_unstemmed Performance of selective and differential media in the primary isolation of yeasts from different biological samples
title_sort Performance of selective and differential media in the primary isolation of yeasts from different biological samples
author Silva, Jaqueline Otero
author_facet Silva, Jaqueline Otero
Franceschini, Silvio Antônio
Lavrador, Marco Aurélio Sicchiroli
Candido, Regina Célia
author_role author
author2 Franceschini, Silvio Antônio
Lavrador, Marco Aurélio Sicchiroli
Candido, Regina Célia
author2_role author
author
author
dc.contributor.none.fl_str_mv Lab. I de Ribeirão Preto
Univ. do Estado de São Paulo
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Silva, Jaqueline Otero
Franceschini, Silvio Antônio
Lavrador, Marco Aurélio Sicchiroli
Candido, Regina Célia
dc.subject.por.fl_str_mv Biggy agar
Candida
CHROMagar Candida
Culture media
Pagano Levin agar
Yeasts
topic Biggy agar
Candida
CHROMagar Candida
Culture media
Pagano Levin agar
Yeasts
description In view of the increase in yeast infections, especially polymicrobial ones, differential culture media have acquired increasing importance. The present study evaluated the Sabouraud chloramphenicol, Biggy agar, Pagano Levin agar and CHROMagar Candida media in terms of isolation, number of yeast colony forming units per plate, and inhibition of bacteria and filamentous fungi. To this end, we used 223 biological samples, including feces, and oral, vaginal and anal mucosae from 86 patients presenting or not symptoms of fungal infections. The four media did not differ significantly in terms of detection of yeast-positive cultures. The number of colony forming units per plate ranged from zero to 2.380, with a predominance of counts of 1 to 9 colonies per plate. No significant differences were observed among the four culture media in terms of number of colonies counted, for each kind of biological material. Fifteen species belonging to the genera Candida, Saccharomyces, Cryptococcus, Trichosporon and Rhodotorula were isolated, with C. albicans being the predominant species, followed by C. parapsilosis and R. rubra. CHROMagar Candida and Biggy agar were complementary in the isolation of the different species and favored a greater recovery of polymicrobial cultures. Pagano Levin agar isolated the smallest variety of species. Sabouraud chloramphenicol agar was the least effective in terms of bacterial inhibition and favored a greater development of filamentous fungi. The results suggest that more than one culture medium should be used for an adequate primary isolation.
publishDate 2004
dc.date.none.fl_str_mv 2004-01-01
2022-04-29T08:42:55Z
2022-04-29T08:42:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1023/B:MYCO.0000012223.38967.7d
Mycopathologia, v. 157, n. 1, p. 29-36, 2004.
0301-486X
http://hdl.handle.net/11449/230970
10.1023/B:MYCO.0000012223.38967.7d
2-s2.0-1242310597
url http://dx.doi.org/10.1023/B:MYCO.0000012223.38967.7d
http://hdl.handle.net/11449/230970
identifier_str_mv Mycopathologia, v. 157, n. 1, p. 29-36, 2004.
0301-486X
10.1023/B:MYCO.0000012223.38967.7d
2-s2.0-1242310597
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Mycopathologia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 29-36
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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