Hepatozoon parasites (Apicomplexa: Hepatozoidae) in fish Hoplias aimara (Characiformes, Erythrinidae) from the Eastern Amazon, Brazil

Detalhes bibliográficos
Autor(a) principal: Cardoso, Wagner Amanajás
Data de Publicação: 2022
Outros Autores: Perles, Lívia [UNESP], Picelli, Amanda Maria, Correa, Jamille Karina Coelho, André, Marcos Rogério [UNESP], Viana, Lúcio André
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00436-022-07462-2
http://hdl.handle.net/11449/230404
Resumo: This study reports the occurrence of parasites belonging to the Hepatozoon genus in fish Hoplias aimara from the Eastern Amazon. Fish (n = 54) were sampled from the Falsino River, located in the Amapá National Forest (FLONA), in the state of Amapá, northern Brazil. Fresh liver preparations were examined in the field between a slide and a coverslip under a light microscope. Cysts containing Hepatozoon cystozoites were observed in the liver of 5 (9%) out of 54 H. aimara individuals. The cysts were ovoid (mean dimensions 10.28 × 9.8 μm), presenting up to four elongated cystozoites (mean dimensions 11.04 × 1.68 μm), containing 1 to 4 residual bodies of different sizes. A single liver sample containing cysts was submitted to DNA extraction and PCR analyses based on a fragment of the 18S rRNA gene. The sequencing revealed a 465 bp fragment exhibiting 99% query coverage, 0.0 E-value, and 98.7% identity with Hepatozoon caimani (MF322538 and MF322539), detected in caimans (Caiman yacare) from Brazil. This is the first report of the occurrence of cysts containing Hepatozoon cystozoites in free-living fishes.
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spelling Hepatozoon parasites (Apicomplexa: Hepatozoidae) in fish Hoplias aimara (Characiformes, Erythrinidae) from the Eastern Amazon, Brazil18S rRNA geneFloresta Nacional do AmapáParatenic hostsTrophic transmissionThis study reports the occurrence of parasites belonging to the Hepatozoon genus in fish Hoplias aimara from the Eastern Amazon. Fish (n = 54) were sampled from the Falsino River, located in the Amapá National Forest (FLONA), in the state of Amapá, northern Brazil. Fresh liver preparations were examined in the field between a slide and a coverslip under a light microscope. Cysts containing Hepatozoon cystozoites were observed in the liver of 5 (9%) out of 54 H. aimara individuals. The cysts were ovoid (mean dimensions 10.28 × 9.8 μm), presenting up to four elongated cystozoites (mean dimensions 11.04 × 1.68 μm), containing 1 to 4 residual bodies of different sizes. A single liver sample containing cysts was submitted to DNA extraction and PCR analyses based on a fragment of the 18S rRNA gene. The sequencing revealed a 465 bp fragment exhibiting 99% query coverage, 0.0 E-value, and 98.7% identity with Hepatozoon caimani (MF322538 and MF322539), detected in caimans (Caiman yacare) from Brazil. This is the first report of the occurrence of cysts containing Hepatozoon cystozoites in free-living fishes.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Programa de Pós-Graduação Em Biodiversidade Tropical Universidade Federal do Amapá–UNIFAP, APLaboratório de Imunoparasitologia Departamento de Patologia Reprodução E Saúde Única Faculdade de Ciências Agrárias E Veterinárias–Universidade Estadual Paulista (FCAV/UNESP), SPPrograma de Pós-Graduação Em Biologia Experimental Fundação Universidade Federal de Rondônia, ROLaboratório de Estudos Morfofisiológicos E Parasitários Departamento de Ciências Biológicas E da Saúde Universidade Federal do Amapá – UNIFAP, APLaboratório de Imunoparasitologia Departamento de Patologia Reprodução E Saúde Única Faculdade de Ciências Agrárias E Veterinárias–Universidade Estadual Paulista (FCAV/UNESP), SPCNPq: 429.132/2016-6Universidade Federal do Amapá–UNIFAPUniversidade Estadual Paulista (UNESP)Fundação Universidade Federal de RondôniaUniversidade Federal do Amapá – UNIFAPCardoso, Wagner AmanajásPerles, Lívia [UNESP]Picelli, Amanda MariaCorrea, Jamille Karina CoelhoAndré, Marcos Rogério [UNESP]Viana, Lúcio André2022-04-29T08:39:48Z2022-04-29T08:39:48Z2022-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1041-1046http://dx.doi.org/10.1007/s00436-022-07462-2Parasitology Research, v. 121, n. 3, p. 1041-1046, 2022.1432-19550932-0113http://hdl.handle.net/11449/23040410.1007/s00436-022-07462-22-s2.0-85124753272Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengParasitology Researchinfo:eu-repo/semantics/openAccess2024-06-07T13:02:38Zoai:repositorio.unesp.br:11449/230404Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:56:56.352406Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Hepatozoon parasites (Apicomplexa: Hepatozoidae) in fish Hoplias aimara (Characiformes, Erythrinidae) from the Eastern Amazon, Brazil
title Hepatozoon parasites (Apicomplexa: Hepatozoidae) in fish Hoplias aimara (Characiformes, Erythrinidae) from the Eastern Amazon, Brazil
spellingShingle Hepatozoon parasites (Apicomplexa: Hepatozoidae) in fish Hoplias aimara (Characiformes, Erythrinidae) from the Eastern Amazon, Brazil
Cardoso, Wagner Amanajás
18S rRNA gene
Floresta Nacional do Amapá
Paratenic hosts
Trophic transmission
title_short Hepatozoon parasites (Apicomplexa: Hepatozoidae) in fish Hoplias aimara (Characiformes, Erythrinidae) from the Eastern Amazon, Brazil
title_full Hepatozoon parasites (Apicomplexa: Hepatozoidae) in fish Hoplias aimara (Characiformes, Erythrinidae) from the Eastern Amazon, Brazil
title_fullStr Hepatozoon parasites (Apicomplexa: Hepatozoidae) in fish Hoplias aimara (Characiformes, Erythrinidae) from the Eastern Amazon, Brazil
title_full_unstemmed Hepatozoon parasites (Apicomplexa: Hepatozoidae) in fish Hoplias aimara (Characiformes, Erythrinidae) from the Eastern Amazon, Brazil
title_sort Hepatozoon parasites (Apicomplexa: Hepatozoidae) in fish Hoplias aimara (Characiformes, Erythrinidae) from the Eastern Amazon, Brazil
author Cardoso, Wagner Amanajás
author_facet Cardoso, Wagner Amanajás
Perles, Lívia [UNESP]
Picelli, Amanda Maria
Correa, Jamille Karina Coelho
André, Marcos Rogério [UNESP]
Viana, Lúcio André
author_role author
author2 Perles, Lívia [UNESP]
Picelli, Amanda Maria
Correa, Jamille Karina Coelho
André, Marcos Rogério [UNESP]
Viana, Lúcio André
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal do Amapá–UNIFAP
Universidade Estadual Paulista (UNESP)
Fundação Universidade Federal de Rondônia
Universidade Federal do Amapá – UNIFAP
dc.contributor.author.fl_str_mv Cardoso, Wagner Amanajás
Perles, Lívia [UNESP]
Picelli, Amanda Maria
Correa, Jamille Karina Coelho
André, Marcos Rogério [UNESP]
Viana, Lúcio André
dc.subject.por.fl_str_mv 18S rRNA gene
Floresta Nacional do Amapá
Paratenic hosts
Trophic transmission
topic 18S rRNA gene
Floresta Nacional do Amapá
Paratenic hosts
Trophic transmission
description This study reports the occurrence of parasites belonging to the Hepatozoon genus in fish Hoplias aimara from the Eastern Amazon. Fish (n = 54) were sampled from the Falsino River, located in the Amapá National Forest (FLONA), in the state of Amapá, northern Brazil. Fresh liver preparations were examined in the field between a slide and a coverslip under a light microscope. Cysts containing Hepatozoon cystozoites were observed in the liver of 5 (9%) out of 54 H. aimara individuals. The cysts were ovoid (mean dimensions 10.28 × 9.8 μm), presenting up to four elongated cystozoites (mean dimensions 11.04 × 1.68 μm), containing 1 to 4 residual bodies of different sizes. A single liver sample containing cysts was submitted to DNA extraction and PCR analyses based on a fragment of the 18S rRNA gene. The sequencing revealed a 465 bp fragment exhibiting 99% query coverage, 0.0 E-value, and 98.7% identity with Hepatozoon caimani (MF322538 and MF322539), detected in caimans (Caiman yacare) from Brazil. This is the first report of the occurrence of cysts containing Hepatozoon cystozoites in free-living fishes.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-29T08:39:48Z
2022-04-29T08:39:48Z
2022-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00436-022-07462-2
Parasitology Research, v. 121, n. 3, p. 1041-1046, 2022.
1432-1955
0932-0113
http://hdl.handle.net/11449/230404
10.1007/s00436-022-07462-2
2-s2.0-85124753272
url http://dx.doi.org/10.1007/s00436-022-07462-2
http://hdl.handle.net/11449/230404
identifier_str_mv Parasitology Research, v. 121, n. 3, p. 1041-1046, 2022.
1432-1955
0932-0113
10.1007/s00436-022-07462-2
2-s2.0-85124753272
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Parasitology Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1041-1046
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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