A new surgical technique to treat corneal perforations using amniotic membrane and surgical adhesive

Detalhes bibliográficos
Autor(a) principal: Pontes, Kelly Cristine de Souza
Data de Publicação: 2014
Outros Autores: Borges, Andréa Pacheco Batista, Eleotério, Renato Barros, Frazão, Ana Carolina do Nascimento, Machado, Daniel Portela Dias, Reis, Emily Correna Carlo, Sepúlveda, Rodrigo Viana, Duarte, Tarcísio de Souza, Laus, José Luiz [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1678-41625707
http://hdl.handle.net/11449/114167
Resumo: The objectives of this study were to compare surgical techniques and the effects of using n-butyl 2-cyanoacrylate and bovine amniotic membrane to repair perforated lesions in corneas. Penetrating keratoplasty was performed in sixty New Zealand White rabbits under general anesthesia. Group 1 (G1) was treated with n-butyl 2-cyanoacrylate, group 2 (G2) received a fragment of amniotic membrane through the anterior chamber and application of n-butyl 2-cyanoacrylate over the lesion, group 3 (G3) was treated with the same technique as G2 with the addition of an amniotic membrane bandage covering the cornea and sutured in the limbus region, and group 4 (G4) was treated with an amniotic membrane sutured to the lesion and an amniotic membrane bandage sutured in the limbus region. Clinical, histological and histomorphometric examinations of the corneas were performed. The membrane acted as a barrier for aqueous humor in G2 and G3, thereby keeping the surface dry for adhesive application; it also prevented the adhesive from contacting intraocular structures. The groups treated with amniotic membrane and surgical adhesive showed better results than the groups treated with either material alone. Thus, the combination of the membrane with the adhesive is recommended for this type of lesion.
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spelling A new surgical technique to treat corneal perforations using amniotic membrane and surgical adhesiveNova técnica cirúrgica para tratamento de perfuração de córnea utilizando membrana amniótica e adesivo cirúrgicoCoelhosCórneaCianoacrilatoGlicerinaCirurgia oftálmicaRabbitsCorneaCyanoacrylateGlycerinOphthalmic surgeryThe objectives of this study were to compare surgical techniques and the effects of using n-butyl 2-cyanoacrylate and bovine amniotic membrane to repair perforated lesions in corneas. Penetrating keratoplasty was performed in sixty New Zealand White rabbits under general anesthesia. Group 1 (G1) was treated with n-butyl 2-cyanoacrylate, group 2 (G2) received a fragment of amniotic membrane through the anterior chamber and application of n-butyl 2-cyanoacrylate over the lesion, group 3 (G3) was treated with the same technique as G2 with the addition of an amniotic membrane bandage covering the cornea and sutured in the limbus region, and group 4 (G4) was treated with an amniotic membrane sutured to the lesion and an amniotic membrane bandage sutured in the limbus region. Clinical, histological and histomorphometric examinations of the corneas were performed. The membrane acted as a barrier for aqueous humor in G2 and G3, thereby keeping the surface dry for adhesive application; it also prevented the adhesive from contacting intraocular structures. The groups treated with amniotic membrane and surgical adhesive showed better results than the groups treated with either material alone. Thus, the combination of the membrane with the adhesive is recommended for this type of lesion.Os objetivos deste estudo foram comparar técnicas cirúrgicas e efeitos do n-butil 2-cianoacrilato e da membrana amniótica bovina na reparação de lesões perfuradas em córneas. Sessenta coelhos da raça Nova Zelândia Branca foram submetidos à ceratoplastia penetrante sob anestesia geral. O grupo 1 (G1) foi tratado com n-butil 2-cianoacrilato; o grupo 2 (G2) recebeu um fragmento de membrana amniótica pela câmara anterior e aplicação de n-butil 2-cianoacrilato sobre a lesão; o grupo 3 (G3) foi tratado com a mesma técnica aplicada ao G2, adicionando-se uma bandagem de membrana amniótica cobrindo a córnea e suturada à região do limbo; e o grupo 4 (G4) foi tratado com membrana amniótica suturada nas bordas da lesão e bandagem de membrana amniótica suturada na região do limbo. Foram realizados exames clínico, histológico e histomorfométrico. A membrana atuou como barreira contra o extravasamento do humor aquoso nos grupos 2 e 3, manteve a superfície seca para posterior aplicação do adesivo e impediu o contato do adesivo com as estruturas intraoculares. Os grupos tratados com o adesivo associado à membrana amniótica demonstraram melhores resultados do que aqueles tratados com cada material isoladamente. Assim, a combinação da membrana com o adesivo é indicada neste tipo de lesão.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Federal de ViçosaUNESP Faculdade de Ciências Agrárias e VeterináriasUNESP Faculdade de Ciências Agrárias e VeterináriasUniversidade Federal de Minas Gerais, Escola de VeterináriaUniversidade Federal de Viçosa (UFV)Universidade Estadual Paulista (Unesp)Pontes, Kelly Cristine de SouzaBorges, Andréa Pacheco BatistaEleotério, Renato BarrosFrazão, Ana Carolina do NascimentoMachado, Daniel Portela DiasReis, Emily Correna CarloSepúlveda, Rodrigo VianaDuarte, Tarcísio de SouzaLaus, José Luiz [UNESP]2015-02-02T12:39:18Z2015-02-02T12:39:18Z2014-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article655-664application/pdfhttp://dx.doi.org/10.1590/1678-41625707Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 66, n. 3, p. 655-664, 2014.0102-0935http://hdl.handle.net/11449/11416710.1590/1678-41625707S0102-09352014000300655S0102-09352014000300655.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivo Brasileiro de Medicina Veterinária e Zootecnia0.2860,248info:eu-repo/semantics/openAccess2024-06-06T14:10:47Zoai:repositorio.unesp.br:11449/114167Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:23:29.510616Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv A new surgical technique to treat corneal perforations using amniotic membrane and surgical adhesive
Nova técnica cirúrgica para tratamento de perfuração de córnea utilizando membrana amniótica e adesivo cirúrgico
title A new surgical technique to treat corneal perforations using amniotic membrane and surgical adhesive
spellingShingle A new surgical technique to treat corneal perforations using amniotic membrane and surgical adhesive
Pontes, Kelly Cristine de Souza
Coelhos
Córnea
Cianoacrilato
Glicerina
Cirurgia oftálmica
Rabbits
Cornea
Cyanoacrylate
Glycerin
Ophthalmic surgery
title_short A new surgical technique to treat corneal perforations using amniotic membrane and surgical adhesive
title_full A new surgical technique to treat corneal perforations using amniotic membrane and surgical adhesive
title_fullStr A new surgical technique to treat corneal perforations using amniotic membrane and surgical adhesive
title_full_unstemmed A new surgical technique to treat corneal perforations using amniotic membrane and surgical adhesive
title_sort A new surgical technique to treat corneal perforations using amniotic membrane and surgical adhesive
author Pontes, Kelly Cristine de Souza
author_facet Pontes, Kelly Cristine de Souza
Borges, Andréa Pacheco Batista
Eleotério, Renato Barros
Frazão, Ana Carolina do Nascimento
Machado, Daniel Portela Dias
Reis, Emily Correna Carlo
Sepúlveda, Rodrigo Viana
Duarte, Tarcísio de Souza
Laus, José Luiz [UNESP]
author_role author
author2 Borges, Andréa Pacheco Batista
Eleotério, Renato Barros
Frazão, Ana Carolina do Nascimento
Machado, Daniel Portela Dias
Reis, Emily Correna Carlo
Sepúlveda, Rodrigo Viana
Duarte, Tarcísio de Souza
Laus, José Luiz [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Viçosa (UFV)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Pontes, Kelly Cristine de Souza
Borges, Andréa Pacheco Batista
Eleotério, Renato Barros
Frazão, Ana Carolina do Nascimento
Machado, Daniel Portela Dias
Reis, Emily Correna Carlo
Sepúlveda, Rodrigo Viana
Duarte, Tarcísio de Souza
Laus, José Luiz [UNESP]
dc.subject.por.fl_str_mv Coelhos
Córnea
Cianoacrilato
Glicerina
Cirurgia oftálmica
Rabbits
Cornea
Cyanoacrylate
Glycerin
Ophthalmic surgery
topic Coelhos
Córnea
Cianoacrilato
Glicerina
Cirurgia oftálmica
Rabbits
Cornea
Cyanoacrylate
Glycerin
Ophthalmic surgery
description The objectives of this study were to compare surgical techniques and the effects of using n-butyl 2-cyanoacrylate and bovine amniotic membrane to repair perforated lesions in corneas. Penetrating keratoplasty was performed in sixty New Zealand White rabbits under general anesthesia. Group 1 (G1) was treated with n-butyl 2-cyanoacrylate, group 2 (G2) received a fragment of amniotic membrane through the anterior chamber and application of n-butyl 2-cyanoacrylate over the lesion, group 3 (G3) was treated with the same technique as G2 with the addition of an amniotic membrane bandage covering the cornea and sutured in the limbus region, and group 4 (G4) was treated with an amniotic membrane sutured to the lesion and an amniotic membrane bandage sutured in the limbus region. Clinical, histological and histomorphometric examinations of the corneas were performed. The membrane acted as a barrier for aqueous humor in G2 and G3, thereby keeping the surface dry for adhesive application; it also prevented the adhesive from contacting intraocular structures. The groups treated with amniotic membrane and surgical adhesive showed better results than the groups treated with either material alone. Thus, the combination of the membrane with the adhesive is recommended for this type of lesion.
publishDate 2014
dc.date.none.fl_str_mv 2014-06-01
2015-02-02T12:39:18Z
2015-02-02T12:39:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1678-41625707
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 66, n. 3, p. 655-664, 2014.
0102-0935
http://hdl.handle.net/11449/114167
10.1590/1678-41625707
S0102-09352014000300655
S0102-09352014000300655.pdf
url http://dx.doi.org/10.1590/1678-41625707
http://hdl.handle.net/11449/114167
identifier_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 66, n. 3, p. 655-664, 2014.
0102-0935
10.1590/1678-41625707
S0102-09352014000300655
S0102-09352014000300655.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia
0.286
0,248
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 655-664
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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