Characterization of novel Acidobacteria exopolysaccharides with potential industrial and ecological applications
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/srep41193 http://hdl.handle.net/11449/169405 |
Resumo: | Acidobacteria have been described as one of the most abundant and ubiquitous bacterial phyla in soil. However, factors contributing to this ecological success are not well elucidated mainly due to difficulties in bacterial isolation. Acidobacteria may be able to survive for long periods in soil due to protection provided by secreted extracellular polymeric substances that include exopolysaccharides (EPSs). Here we present the first study to characterize EPSs derived from two strains of Acidobacteria from subdivision 1 belonging to Granulicella sp. EPS are unique heteropolysaccharides containing mannose, glucose, galactose and xylose as major components, and are modified with carboxyl and methoxyl functional groups that we characterized by Fourier transform infrared (FTIR) spectroscopy. Both EPS compounds we identified can efficiently emulsify various oils (sunflower seed, diesel, and liquid paraffin) and hydrocarbons (toluene and hexane). Moreover, the emulsions are more thermostable over time than those of commercialized xanthan. Acidobacterial EPS can now be explored as a source of biopolymers that may be attractive and valuable for industrial applications due to their natural origin, sustainability, biodegradability and low toxicity. |
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Characterization of novel Acidobacteria exopolysaccharides with potential industrial and ecological applicationsAcidobacteria have been described as one of the most abundant and ubiquitous bacterial phyla in soil. However, factors contributing to this ecological success are not well elucidated mainly due to difficulties in bacterial isolation. Acidobacteria may be able to survive for long periods in soil due to protection provided by secreted extracellular polymeric substances that include exopolysaccharides (EPSs). Here we present the first study to characterize EPSs derived from two strains of Acidobacteria from subdivision 1 belonging to Granulicella sp. EPS are unique heteropolysaccharides containing mannose, glucose, galactose and xylose as major components, and are modified with carboxyl and methoxyl functional groups that we characterized by Fourier transform infrared (FTIR) spectroscopy. Both EPS compounds we identified can efficiently emulsify various oils (sunflower seed, diesel, and liquid paraffin) and hydrocarbons (toluene and hexane). Moreover, the emulsions are more thermostable over time than those of commercialized xanthan. Acidobacterial EPS can now be explored as a source of biopolymers that may be attractive and valuable for industrial applications due to their natural origin, sustainability, biodegradability and low toxicity.Netherlands Institute of Ecology (NIOO-KNAW) Department of Microbial Ecology, P.O. Box 50São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, Rod. Prof. Paulo Donato Castellane km 5Embrapa Instrumentação, Rua XV de Novembro, 1452Faculdade de Ci'ncias e Tecnologia (Unesp) Departmento de Química e Bioquímica, Rua Roberto Símonsen, 305São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, Rod. Prof. Paulo Donato Castellane km 5Faculdade de Ci'ncias e Tecnologia (Unesp) Departmento de Química e Bioquímica, Rua Roberto Símonsen, 305Netherlands Institute of Ecology (NIOO-KNAW)Universidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Kielak, Anna M.Castellane, Tereza C.L. [UNESP]Campanharo, Joao C. [UNESP]Colnago, Luiz A.Costa, Ohana Y.A.Da Silva, Maria L. Corradi [UNESP]Van Veen, Johannes A.Lemos, Eliana G.M. [UNESP]Kuramae, Eiko E.2018-12-11T16:45:43Z2018-12-11T16:45:43Z2017-01-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1038/srep41193Scientific Reports, v. 7.2045-2322http://hdl.handle.net/11449/16940510.1038/srep411932-s2.0-850109105372-s2.0-85010910537.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reports1,533info:eu-repo/semantics/openAccess2023-11-07T06:10:58Zoai:repositorio.unesp.br:11449/169405Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:04:42.314535Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Characterization of novel Acidobacteria exopolysaccharides with potential industrial and ecological applications |
title |
Characterization of novel Acidobacteria exopolysaccharides with potential industrial and ecological applications |
spellingShingle |
Characterization of novel Acidobacteria exopolysaccharides with potential industrial and ecological applications Kielak, Anna M. |
title_short |
Characterization of novel Acidobacteria exopolysaccharides with potential industrial and ecological applications |
title_full |
Characterization of novel Acidobacteria exopolysaccharides with potential industrial and ecological applications |
title_fullStr |
Characterization of novel Acidobacteria exopolysaccharides with potential industrial and ecological applications |
title_full_unstemmed |
Characterization of novel Acidobacteria exopolysaccharides with potential industrial and ecological applications |
title_sort |
Characterization of novel Acidobacteria exopolysaccharides with potential industrial and ecological applications |
author |
Kielak, Anna M. |
author_facet |
Kielak, Anna M. Castellane, Tereza C.L. [UNESP] Campanharo, Joao C. [UNESP] Colnago, Luiz A. Costa, Ohana Y.A. Da Silva, Maria L. Corradi [UNESP] Van Veen, Johannes A. Lemos, Eliana G.M. [UNESP] Kuramae, Eiko E. |
author_role |
author |
author2 |
Castellane, Tereza C.L. [UNESP] Campanharo, Joao C. [UNESP] Colnago, Luiz A. Costa, Ohana Y.A. Da Silva, Maria L. Corradi [UNESP] Van Veen, Johannes A. Lemos, Eliana G.M. [UNESP] Kuramae, Eiko E. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Netherlands Institute of Ecology (NIOO-KNAW) Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Kielak, Anna M. Castellane, Tereza C.L. [UNESP] Campanharo, Joao C. [UNESP] Colnago, Luiz A. Costa, Ohana Y.A. Da Silva, Maria L. Corradi [UNESP] Van Veen, Johannes A. Lemos, Eliana G.M. [UNESP] Kuramae, Eiko E. |
description |
Acidobacteria have been described as one of the most abundant and ubiquitous bacterial phyla in soil. However, factors contributing to this ecological success are not well elucidated mainly due to difficulties in bacterial isolation. Acidobacteria may be able to survive for long periods in soil due to protection provided by secreted extracellular polymeric substances that include exopolysaccharides (EPSs). Here we present the first study to characterize EPSs derived from two strains of Acidobacteria from subdivision 1 belonging to Granulicella sp. EPS are unique heteropolysaccharides containing mannose, glucose, galactose and xylose as major components, and are modified with carboxyl and methoxyl functional groups that we characterized by Fourier transform infrared (FTIR) spectroscopy. Both EPS compounds we identified can efficiently emulsify various oils (sunflower seed, diesel, and liquid paraffin) and hydrocarbons (toluene and hexane). Moreover, the emulsions are more thermostable over time than those of commercialized xanthan. Acidobacterial EPS can now be explored as a source of biopolymers that may be attractive and valuable for industrial applications due to their natural origin, sustainability, biodegradability and low toxicity. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-24 2018-12-11T16:45:43Z 2018-12-11T16:45:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/srep41193 Scientific Reports, v. 7. 2045-2322 http://hdl.handle.net/11449/169405 10.1038/srep41193 2-s2.0-85010910537 2-s2.0-85010910537.pdf |
url |
http://dx.doi.org/10.1038/srep41193 http://hdl.handle.net/11449/169405 |
identifier_str_mv |
Scientific Reports, v. 7. 2045-2322 10.1038/srep41193 2-s2.0-85010910537 2-s2.0-85010910537.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports 1,533 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128752104767488 |