Utiliza????o de nitrato por Telmatobacter sp. AB60 Acidobacteria
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UCB |
Texto Completo: | https://bdtd.ucb.br:8443/jspui/handle/tede/2701 |
Resumo: | Acidobacteria physiology and their role in the biogeochemical cycles remain relatively unknown compared to another widespread phylum such as Proteobacteria. Although some studies have focused on carbon usage by Acidobacteria other aspects of their metabolism were neglected as their use of nitrogen or phosphorous. We have previously obtained a few isolates of Acidobacteriia from the Cerrado, which soils are acidic and poor in phosphorous and nitrate. The objective of this work was to investigate the nitrogen metabolism pathways present in the genome of the isolate Telmatobacter sp. AB60, an Acidobacteria from the Class Acidobacteriia. The genome of Telmatobacter sp. AB60 was previously sequenced revealing the presence of six genes involved in assimilatory nitrogen metabolism, such as nasAB e nirA (nitrate and nitrite reductase, respectively). Comparison of the deduced amino acid sequences of NasA and NirA revealed that the Acidobacteria genes more closely related to each other than to any other phylum. Nitrate and nitrite were used as sole nitrogen source separately and in combination, in oxic and anoxic conditions. The isolate AB60 grew on nitrate as sole nitrogen source and lactate as carbon and electron source. Growth was observed both in the presence and absence of oxygen. Assimilative nitrate reduction was observed for the first time in Acidobacteria, since isolate AB60 can use nitrate and lactate for growth and does not release of ammonia in the supernatant of the culture. On the other hand, the use of 2 mM of nitrite as sole nitrogen source inhibited the growth of isolate AB60.The next step of this work will be to test gene regulation in the presence of nitrate and nitrite, to detect other metabolic products during growth, an then broaden our knowledge of the Acidobacteriia??s role in the nitrogen cycle of the Cerrado. |
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Barreto, Cristine Chaveshttp://lattes.cnpq.br/9973148799581550http://lattes.cnpq.br/2604711750939666Moura, Kamila Suellen Paiva de2020-02-21T18:27:04Z2020-08-26MOURA, Kamila Suellen Paiva de. Utiliza????o de nitrato por Telmatobacter sp. AB60 Acidobacteria. 2020. 53 f. Disserta????o (Programa Stricto Sensu em Ci??ncias Gen??micas e Biotecnologia) - Universidade Cat??lica de Bras??lia, 2019.https://bdtd.ucb.br:8443/jspui/handle/tede/2701Acidobacteria physiology and their role in the biogeochemical cycles remain relatively unknown compared to another widespread phylum such as Proteobacteria. Although some studies have focused on carbon usage by Acidobacteria other aspects of their metabolism were neglected as their use of nitrogen or phosphorous. We have previously obtained a few isolates of Acidobacteriia from the Cerrado, which soils are acidic and poor in phosphorous and nitrate. The objective of this work was to investigate the nitrogen metabolism pathways present in the genome of the isolate Telmatobacter sp. AB60, an Acidobacteria from the Class Acidobacteriia. The genome of Telmatobacter sp. AB60 was previously sequenced revealing the presence of six genes involved in assimilatory nitrogen metabolism, such as nasAB e nirA (nitrate and nitrite reductase, respectively). Comparison of the deduced amino acid sequences of NasA and NirA revealed that the Acidobacteria genes more closely related to each other than to any other phylum. Nitrate and nitrite were used as sole nitrogen source separately and in combination, in oxic and anoxic conditions. The isolate AB60 grew on nitrate as sole nitrogen source and lactate as carbon and electron source. Growth was observed both in the presence and absence of oxygen. Assimilative nitrate reduction was observed for the first time in Acidobacteria, since isolate AB60 can use nitrate and lactate for growth and does not release of ammonia in the supernatant of the culture. On the other hand, the use of 2 mM of nitrite as sole nitrogen source inhibited the growth of isolate AB60.The next step of this work will be to test gene regulation in the presence of nitrate and nitrite, to detect other metabolic products during growth, an then broaden our knowledge of the Acidobacteriia??s role in the nitrogen cycle of the Cerrado.A fisiologia das acidobact??rias e o seu papel nos ciclos biogeoqu??micos permanecem relativamente desconhecidos em compara????o com filos como Proteobacteria, por exemplo. Embora estudos fisiologicos sobre utiliza????o de carbono j?? tenham sido realizados, outros aspectos do seu metabolismo foram negligenciados como o uso de nitrog??nio ou f??sforo. Alguns isolados de Acidobacteriia do Cerrado, cujos solos s??o ??cidos e pobres em f??sforo e nitrato foram obtidos em estudo anterior. O objetivo do presente trabalho foi estudar a utiliza????o de nitrato por Telmatobacter sp. AB60, uma Acidobacteria da Classe Acidobacteriia. O genoma de Telmatobacter sp. foi previamente sequenciado e a sua an??lise revelou a presen??a de seis genes envolvidos na redu????o assimilativa de nitrato e nitrito, entre eles nasAB e nirA (nitrato e nitrito redutase, respectivamente). A compara????o das sequ??ncias de amino??cidos deduzidas da subunidade catalitica de NasA e NirA mostrou que os genes de Acidobacteria se relacionam mais intimamente entre si do que com qualquer outro filo. Nitrato e nitrito foram usados separadamente ou em combina????o para an??lise de crescimento, em condi????es ??xicas e an??xicas. O isolado AB60 foi capaz de crescer em nitrato como ??nica fonte de nitrog??nio e lactato como fonte de carbono e el??trons, tanto com presen??a de oxig??nio como em aus??ncia. Foi demonstrado pela primeira vez a capacidade de redu????o assimilativa de Acidobact??ria, pois observou-se o consumo de nitrato e lactato durante o crescimento sem a libera????o de amonia no sobrenadante. Por outro lado, a concentra????o de 2 mM de nitrito como unica fonte de nitrogenio foi inibit??ria para o crescimento de AB60. O pr??ximo passo deste trabalho ser?? verificar a regula????o da express??o g??nica, detectar outros produtos do metabolismo e ampliar nosso conhecimento sobre o papel da Acidobacteriia no ciclo do nitrog??nio do Cerrado.Submitted by Sara Ribeiro (sara.ribeiro@ucb.br) on 2020-02-21T18:26:48Z No. of bitstreams: 1 KamilaSuellenPaivadeMouraDissertacao2019.pdf: 2066624 bytes, checksum: 3a2082bd9a8b66d833a64424c0b48011 (MD5)Approved for entry into archive by Sara Ribeiro (sara.ribeiro@ucb.br) on 2020-02-21T18:27:04Z (GMT) No. of bitstreams: 1 KamilaSuellenPaivadeMouraDissertacao2019.pdf: 2066624 bytes, checksum: 3a2082bd9a8b66d833a64424c0b48011 (MD5)Made available in DSpace on 2020-02-21T18:27:04Z (GMT). No. of bitstreams: 1 KamilaSuellenPaivadeMouraDissertacao2019.pdf: 2066624 bytes, checksum: 3a2082bd9a8b66d833a64424c0b48011 (MD5) Previous issue date: 2020-08-26application/pdfhttps://200.214.135.178:8443/jspui/retrieve/6926/KamilaSuellenPaivadeMouraDissertacao2019.pdf.jpgporUniversidade Cat??lica de Bras??liaPrograma Stricto Sensu em Ci??ncias Gen??micas e BiotecnologiaUCBBrasilEscola de Sa??de e MedicinaAcidobacteriaCiclo do nitrog??nioNitrogen cycleAcidobacteriaCNPQ::CIENCIAS BIOLOGICASUtiliza????o de nitrato por Telmatobacter sp. 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dc.title.por.fl_str_mv |
Utiliza????o de nitrato por Telmatobacter sp. AB60 Acidobacteria |
title |
Utiliza????o de nitrato por Telmatobacter sp. AB60 Acidobacteria |
spellingShingle |
Utiliza????o de nitrato por Telmatobacter sp. AB60 Acidobacteria Moura, Kamila Suellen Paiva de Acidobacteria Ciclo do nitrog??nio Nitrogen cycle Acidobacteria CNPQ::CIENCIAS BIOLOGICAS |
title_short |
Utiliza????o de nitrato por Telmatobacter sp. AB60 Acidobacteria |
title_full |
Utiliza????o de nitrato por Telmatobacter sp. AB60 Acidobacteria |
title_fullStr |
Utiliza????o de nitrato por Telmatobacter sp. AB60 Acidobacteria |
title_full_unstemmed |
Utiliza????o de nitrato por Telmatobacter sp. AB60 Acidobacteria |
title_sort |
Utiliza????o de nitrato por Telmatobacter sp. AB60 Acidobacteria |
author |
Moura, Kamila Suellen Paiva de |
author_facet |
Moura, Kamila Suellen Paiva de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Barreto, Cristine Chaves |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9973148799581550 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2604711750939666 |
dc.contributor.author.fl_str_mv |
Moura, Kamila Suellen Paiva de |
contributor_str_mv |
Barreto, Cristine Chaves |
dc.subject.por.fl_str_mv |
Acidobacteria Ciclo do nitrog??nio |
topic |
Acidobacteria Ciclo do nitrog??nio Nitrogen cycle Acidobacteria CNPQ::CIENCIAS BIOLOGICAS |
dc.subject.eng.fl_str_mv |
Nitrogen cycle Acidobacteria |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS |
dc.description.abstract.eng.fl_txt_mv |
Acidobacteria physiology and their role in the biogeochemical cycles remain relatively unknown compared to another widespread phylum such as Proteobacteria. Although some studies have focused on carbon usage by Acidobacteria other aspects of their metabolism were neglected as their use of nitrogen or phosphorous. We have previously obtained a few isolates of Acidobacteriia from the Cerrado, which soils are acidic and poor in phosphorous and nitrate. The objective of this work was to investigate the nitrogen metabolism pathways present in the genome of the isolate Telmatobacter sp. AB60, an Acidobacteria from the Class Acidobacteriia. The genome of Telmatobacter sp. AB60 was previously sequenced revealing the presence of six genes involved in assimilatory nitrogen metabolism, such as nasAB e nirA (nitrate and nitrite reductase, respectively). Comparison of the deduced amino acid sequences of NasA and NirA revealed that the Acidobacteria genes more closely related to each other than to any other phylum. Nitrate and nitrite were used as sole nitrogen source separately and in combination, in oxic and anoxic conditions. The isolate AB60 grew on nitrate as sole nitrogen source and lactate as carbon and electron source. Growth was observed both in the presence and absence of oxygen. Assimilative nitrate reduction was observed for the first time in Acidobacteria, since isolate AB60 can use nitrate and lactate for growth and does not release of ammonia in the supernatant of the culture. On the other hand, the use of 2 mM of nitrite as sole nitrogen source inhibited the growth of isolate AB60.The next step of this work will be to test gene regulation in the presence of nitrate and nitrite, to detect other metabolic products during growth, an then broaden our knowledge of the Acidobacteriia??s role in the nitrogen cycle of the Cerrado. |
dc.description.abstract.por.fl_txt_mv |
A fisiologia das acidobact??rias e o seu papel nos ciclos biogeoqu??micos permanecem relativamente desconhecidos em compara????o com filos como Proteobacteria, por exemplo. Embora estudos fisiologicos sobre utiliza????o de carbono j?? tenham sido realizados, outros aspectos do seu metabolismo foram negligenciados como o uso de nitrog??nio ou f??sforo. Alguns isolados de Acidobacteriia do Cerrado, cujos solos s??o ??cidos e pobres em f??sforo e nitrato foram obtidos em estudo anterior. O objetivo do presente trabalho foi estudar a utiliza????o de nitrato por Telmatobacter sp. AB60, uma Acidobacteria da Classe Acidobacteriia. O genoma de Telmatobacter sp. foi previamente sequenciado e a sua an??lise revelou a presen??a de seis genes envolvidos na redu????o assimilativa de nitrato e nitrito, entre eles nasAB e nirA (nitrato e nitrito redutase, respectivamente). A compara????o das sequ??ncias de amino??cidos deduzidas da subunidade catalitica de NasA e NirA mostrou que os genes de Acidobacteria se relacionam mais intimamente entre si do que com qualquer outro filo. Nitrato e nitrito foram usados separadamente ou em combina????o para an??lise de crescimento, em condi????es ??xicas e an??xicas. O isolado AB60 foi capaz de crescer em nitrato como ??nica fonte de nitrog??nio e lactato como fonte de carbono e el??trons, tanto com presen??a de oxig??nio como em aus??ncia. Foi demonstrado pela primeira vez a capacidade de redu????o assimilativa de Acidobact??ria, pois observou-se o consumo de nitrato e lactato durante o crescimento sem a libera????o de amonia no sobrenadante. Por outro lado, a concentra????o de 2 mM de nitrito como unica fonte de nitrogenio foi inibit??ria para o crescimento de AB60. O pr??ximo passo deste trabalho ser?? verificar a regula????o da express??o g??nica, detectar outros produtos do metabolismo e ampliar nosso conhecimento sobre o papel da Acidobacteriia no ciclo do nitrog??nio do Cerrado. |
description |
Acidobacteria physiology and their role in the biogeochemical cycles remain relatively unknown compared to another widespread phylum such as Proteobacteria. Although some studies have focused on carbon usage by Acidobacteria other aspects of their metabolism were neglected as their use of nitrogen or phosphorous. We have previously obtained a few isolates of Acidobacteriia from the Cerrado, which soils are acidic and poor in phosphorous and nitrate. The objective of this work was to investigate the nitrogen metabolism pathways present in the genome of the isolate Telmatobacter sp. AB60, an Acidobacteria from the Class Acidobacteriia. The genome of Telmatobacter sp. AB60 was previously sequenced revealing the presence of six genes involved in assimilatory nitrogen metabolism, such as nasAB e nirA (nitrate and nitrite reductase, respectively). Comparison of the deduced amino acid sequences of NasA and NirA revealed that the Acidobacteria genes more closely related to each other than to any other phylum. Nitrate and nitrite were used as sole nitrogen source separately and in combination, in oxic and anoxic conditions. The isolate AB60 grew on nitrate as sole nitrogen source and lactate as carbon and electron source. Growth was observed both in the presence and absence of oxygen. Assimilative nitrate reduction was observed for the first time in Acidobacteria, since isolate AB60 can use nitrate and lactate for growth and does not release of ammonia in the supernatant of the culture. On the other hand, the use of 2 mM of nitrite as sole nitrogen source inhibited the growth of isolate AB60.The next step of this work will be to test gene regulation in the presence of nitrate and nitrite, to detect other metabolic products during growth, an then broaden our knowledge of the Acidobacteriia??s role in the nitrogen cycle of the Cerrado. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-02-21T18:27:04Z |
dc.date.issued.fl_str_mv |
2020-08-26 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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publishedVersion |
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masterThesis |
dc.identifier.citation.fl_str_mv |
MOURA, Kamila Suellen Paiva de. Utiliza????o de nitrato por Telmatobacter sp. AB60 Acidobacteria. 2020. 53 f. Disserta????o (Programa Stricto Sensu em Ci??ncias Gen??micas e Biotecnologia) - Universidade Cat??lica de Bras??lia, 2019. |
dc.identifier.uri.fl_str_mv |
https://bdtd.ucb.br:8443/jspui/handle/tede/2701 |
identifier_str_mv |
MOURA, Kamila Suellen Paiva de. Utiliza????o de nitrato por Telmatobacter sp. AB60 Acidobacteria. 2020. 53 f. Disserta????o (Programa Stricto Sensu em Ci??ncias Gen??micas e Biotecnologia) - Universidade Cat??lica de Bras??lia, 2019. |
url |
https://bdtd.ucb.br:8443/jspui/handle/tede/2701 |
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por |
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por |
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Universidade Cat??lica de Bras??lia |
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Programa Stricto Sensu em Ci??ncias Gen??micas e Biotecnologia |
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UCB |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Escola de Sa??de e Medicina |
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Universidade Cat??lica de Bras??lia |
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