Hemoplasmas and ticks in cattle from Somalia
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.actatropica.2022.106696 http://hdl.handle.net/11449/247673 |
Resumo: | Hemotropic mycoplasmas (hemoplasmas) are small Gram-negative bacteria that parasitize red blood cells and can cause mild to severe anemia in a wide range of vertebrates, including ruminants. Cattle population in Somalia is around 3.9 million heads, with animals more concentrated around the river areas, mainly in the Juba River and Shabelle River Valleys. Information on hemoplasmas in Sub-Saharan Africa are scarce, mainly in Somalia, where no studies have been performed to date. Accordingly, this study aimed to assess the molecular occurrence of hemoplasmas in 131 cattle blood samples from Somalia. Thirty out of 131 (22.90%; 95% CI: 16.54-30.81%) cattle were infested by ticks: Rhipicephalus pulchellus (68.18%), Amblyomma gemma (18.18%), Amblyomma lepidum (9.09%), Hyalomma marginatum (1.51%), Hyalmomma rufipes (1.51%), and Rhipicephalus pravus (1.51%). A total of 74/131 (56.48%; 95% CI: 47.93-64.67%) cattle were positive for hemotropic Mycoplasma spp. by real-time PCR (qPCR) based on the 16S rRNA gene. Hemoplasma-positive samples were later subjected to species-specific PCR assays for Mycoplasma wenyonii and ‘Candidatus Mycoplasma haematobovis’ based on the 16S rRNA gene. A total of 34/74 (45.94%; 95% CI: 35.07-57.22%) animals were coinfected by both species; 31/74 (41.89%; 95% CI: 31.32-53.26%) and 3/74 (4.05%; 95% CI: 01.39-11.25%) cattle were solely positive to M. wenyonii and ‘Ca. M. haematobovis’, respectively. Six out of 74 (8.1%; 95% CI: 03.77-16.58%) cattle were negative on species-specific conventional PCR assays but tested positive by a semi-nested PCR assay based on the 16S rRNA gene of hemoplasmas. Sequencing of the detected hemotropic Mycoplasma sp. 16S rRNA gene confirmed that animals were infected by M. wenyonii and ‘Ca. M. haematobovis’. To the best of our knowledge, this is the first study on the detection of hemoplasmas in cattle from Somalia. |
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Hemoplasmas and ticks in cattle from SomaliaBovineHemotropic mycoplasmasMycoplasma wenyoniiSub-Saharan Africa‘Candidatus Mycoplasma haematobovisHemotropic mycoplasmas (hemoplasmas) are small Gram-negative bacteria that parasitize red blood cells and can cause mild to severe anemia in a wide range of vertebrates, including ruminants. Cattle population in Somalia is around 3.9 million heads, with animals more concentrated around the river areas, mainly in the Juba River and Shabelle River Valleys. Information on hemoplasmas in Sub-Saharan Africa are scarce, mainly in Somalia, where no studies have been performed to date. Accordingly, this study aimed to assess the molecular occurrence of hemoplasmas in 131 cattle blood samples from Somalia. Thirty out of 131 (22.90%; 95% CI: 16.54-30.81%) cattle were infested by ticks: Rhipicephalus pulchellus (68.18%), Amblyomma gemma (18.18%), Amblyomma lepidum (9.09%), Hyalomma marginatum (1.51%), Hyalmomma rufipes (1.51%), and Rhipicephalus pravus (1.51%). A total of 74/131 (56.48%; 95% CI: 47.93-64.67%) cattle were positive for hemotropic Mycoplasma spp. by real-time PCR (qPCR) based on the 16S rRNA gene. Hemoplasma-positive samples were later subjected to species-specific PCR assays for Mycoplasma wenyonii and ‘Candidatus Mycoplasma haematobovis’ based on the 16S rRNA gene. A total of 34/74 (45.94%; 95% CI: 35.07-57.22%) animals were coinfected by both species; 31/74 (41.89%; 95% CI: 31.32-53.26%) and 3/74 (4.05%; 95% CI: 01.39-11.25%) cattle were solely positive to M. wenyonii and ‘Ca. M. haematobovis’, respectively. Six out of 74 (8.1%; 95% CI: 03.77-16.58%) cattle were negative on species-specific conventional PCR assays but tested positive by a semi-nested PCR assay based on the 16S rRNA gene of hemoplasmas. Sequencing of the detected hemotropic Mycoplasma sp. 16S rRNA gene confirmed that animals were infected by M. wenyonii and ‘Ca. M. haematobovis’. To the best of our knowledge, this is the first study on the detection of hemoplasmas in cattle from Somalia.Universidade Federal do ParanáVector-Borne Diseases Laboratory Departament of Veterinary Medicine Universidade Federal do Paraná (UFPR), ParanáAbrar Research and Training Centre Abrar UniversitySomali One Health Centre Abrar UniversityLaboratório de Imunoparasitologia Departamento de Patologia Reprodução e Saúde Única Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (FCAV/UNESP)Global One Health initiative (GOHi) The Ohio State UniversityLaboratório de Imunoparasitologia Departamento de Patologia Reprodução e Saúde Única Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (FCAV/UNESP)Universidade Federal do Paraná: #23075.042760/2021-59Universidade Federal do Paraná (UFPR)Abrar UniversityUniversidade Estadual Paulista (UNESP)The Ohio State UniversityFerrari, Larissa D.R.Hassan-Kadle, Ahmed A.Collere, Flávia C.M.Coradi, Vanessa S.Ibrahim, Abdalla M.Osman, Aamir M.Shair, Mohamed A.André, Marcos R. [UNESP]Vieira, Thállitha S.W.J.Machado, Rosangela Z. [UNESP]Vieira, Rafael F.C.2023-07-29T13:22:37Z2023-07-29T13:22:37Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.actatropica.2022.106696Acta Tropica, v. 236.1873-62540001-706Xhttp://hdl.handle.net/11449/24767310.1016/j.actatropica.2022.1066962-s2.0-85138804628Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Tropicainfo:eu-repo/semantics/openAccess2023-07-29T13:22:37Zoai:repositorio.unesp.br:11449/247673Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:22:37Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Hemoplasmas and ticks in cattle from Somalia |
title |
Hemoplasmas and ticks in cattle from Somalia |
spellingShingle |
Hemoplasmas and ticks in cattle from Somalia Ferrari, Larissa D.R. Bovine Hemotropic mycoplasmas Mycoplasma wenyonii Sub-Saharan Africa ‘Candidatus Mycoplasma haematobovis |
title_short |
Hemoplasmas and ticks in cattle from Somalia |
title_full |
Hemoplasmas and ticks in cattle from Somalia |
title_fullStr |
Hemoplasmas and ticks in cattle from Somalia |
title_full_unstemmed |
Hemoplasmas and ticks in cattle from Somalia |
title_sort |
Hemoplasmas and ticks in cattle from Somalia |
author |
Ferrari, Larissa D.R. |
author_facet |
Ferrari, Larissa D.R. Hassan-Kadle, Ahmed A. Collere, Flávia C.M. Coradi, Vanessa S. Ibrahim, Abdalla M. Osman, Aamir M. Shair, Mohamed A. André, Marcos R. [UNESP] Vieira, Thállitha S.W.J. Machado, Rosangela Z. [UNESP] Vieira, Rafael F.C. |
author_role |
author |
author2 |
Hassan-Kadle, Ahmed A. Collere, Flávia C.M. Coradi, Vanessa S. Ibrahim, Abdalla M. Osman, Aamir M. Shair, Mohamed A. André, Marcos R. [UNESP] Vieira, Thállitha S.W.J. Machado, Rosangela Z. [UNESP] Vieira, Rafael F.C. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Paraná (UFPR) Abrar University Universidade Estadual Paulista (UNESP) The Ohio State University |
dc.contributor.author.fl_str_mv |
Ferrari, Larissa D.R. Hassan-Kadle, Ahmed A. Collere, Flávia C.M. Coradi, Vanessa S. Ibrahim, Abdalla M. Osman, Aamir M. Shair, Mohamed A. André, Marcos R. [UNESP] Vieira, Thállitha S.W.J. Machado, Rosangela Z. [UNESP] Vieira, Rafael F.C. |
dc.subject.por.fl_str_mv |
Bovine Hemotropic mycoplasmas Mycoplasma wenyonii Sub-Saharan Africa ‘Candidatus Mycoplasma haematobovis |
topic |
Bovine Hemotropic mycoplasmas Mycoplasma wenyonii Sub-Saharan Africa ‘Candidatus Mycoplasma haematobovis |
description |
Hemotropic mycoplasmas (hemoplasmas) are small Gram-negative bacteria that parasitize red blood cells and can cause mild to severe anemia in a wide range of vertebrates, including ruminants. Cattle population in Somalia is around 3.9 million heads, with animals more concentrated around the river areas, mainly in the Juba River and Shabelle River Valleys. Information on hemoplasmas in Sub-Saharan Africa are scarce, mainly in Somalia, where no studies have been performed to date. Accordingly, this study aimed to assess the molecular occurrence of hemoplasmas in 131 cattle blood samples from Somalia. Thirty out of 131 (22.90%; 95% CI: 16.54-30.81%) cattle were infested by ticks: Rhipicephalus pulchellus (68.18%), Amblyomma gemma (18.18%), Amblyomma lepidum (9.09%), Hyalomma marginatum (1.51%), Hyalmomma rufipes (1.51%), and Rhipicephalus pravus (1.51%). A total of 74/131 (56.48%; 95% CI: 47.93-64.67%) cattle were positive for hemotropic Mycoplasma spp. by real-time PCR (qPCR) based on the 16S rRNA gene. Hemoplasma-positive samples were later subjected to species-specific PCR assays for Mycoplasma wenyonii and ‘Candidatus Mycoplasma haematobovis’ based on the 16S rRNA gene. A total of 34/74 (45.94%; 95% CI: 35.07-57.22%) animals were coinfected by both species; 31/74 (41.89%; 95% CI: 31.32-53.26%) and 3/74 (4.05%; 95% CI: 01.39-11.25%) cattle were solely positive to M. wenyonii and ‘Ca. M. haematobovis’, respectively. Six out of 74 (8.1%; 95% CI: 03.77-16.58%) cattle were negative on species-specific conventional PCR assays but tested positive by a semi-nested PCR assay based on the 16S rRNA gene of hemoplasmas. Sequencing of the detected hemotropic Mycoplasma sp. 16S rRNA gene confirmed that animals were infected by M. wenyonii and ‘Ca. M. haematobovis’. To the best of our knowledge, this is the first study on the detection of hemoplasmas in cattle from Somalia. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-01 2023-07-29T13:22:37Z 2023-07-29T13:22:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.actatropica.2022.106696 Acta Tropica, v. 236. 1873-6254 0001-706X http://hdl.handle.net/11449/247673 10.1016/j.actatropica.2022.106696 2-s2.0-85138804628 |
url |
http://dx.doi.org/10.1016/j.actatropica.2022.106696 http://hdl.handle.net/11449/247673 |
identifier_str_mv |
Acta Tropica, v. 236. 1873-6254 0001-706X 10.1016/j.actatropica.2022.106696 2-s2.0-85138804628 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Acta Tropica |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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