GC-TOF/MS-based metabolomics analysis to investigate the changes driven by N-Acetylcysteine in the plant-pathogen Xanthomonas citri subsp. citri
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-021-95113-4 http://hdl.handle.net/11449/233404 |
Resumo: | N-Acetylcysteine (NAC) is an antioxidant, anti-adhesive, and antimicrobial compound. Even though there is much information regarding the role of NAC as an antioxidant and anti-adhesive agent, little is known about its antimicrobial activity. In order to assess its mode of action in bacterial cells, we investigated the metabolic responses triggered by NAC at neutral pH. As a model organism, we chose the Gram-negative plant pathogen Xanthomonas citri subsp. citri (X. citri), the causal agent of citrus canker disease, due to the potential use of NAC as a sustainable molecule against phytopathogens dissemination in citrus cultivated areas. In presence of NAC, cell proliferation was affected after 4 h, but damages to the cell membrane were observed only after 24 h. Targeted metabolite profiling analysis using GC–MS/TOF unravelled that NAC seems to be metabolized by the cells affecting cysteine metabolism. Intriguingly, glutamine, a marker for nitrogen status, was not detected among the cells treated with NAC. The absence of glutamine was followed by a decrease in the levels of the majority of the proteinogenic amino acids, suggesting that the reduced availability of amino acids affect protein synthesis and consequently cell proliferation. |
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GC-TOF/MS-based metabolomics analysis to investigate the changes driven by N-Acetylcysteine in the plant-pathogen Xanthomonas citri subsp. citriN-Acetylcysteine (NAC) is an antioxidant, anti-adhesive, and antimicrobial compound. Even though there is much information regarding the role of NAC as an antioxidant and anti-adhesive agent, little is known about its antimicrobial activity. In order to assess its mode of action in bacterial cells, we investigated the metabolic responses triggered by NAC at neutral pH. As a model organism, we chose the Gram-negative plant pathogen Xanthomonas citri subsp. citri (X. citri), the causal agent of citrus canker disease, due to the potential use of NAC as a sustainable molecule against phytopathogens dissemination in citrus cultivated areas. In presence of NAC, cell proliferation was affected after 4 h, but damages to the cell membrane were observed only after 24 h. Targeted metabolite profiling analysis using GC–MS/TOF unravelled that NAC seems to be metabolized by the cells affecting cysteine metabolism. Intriguingly, glutamine, a marker for nitrogen status, was not detected among the cells treated with NAC. The absence of glutamine was followed by a decrease in the levels of the majority of the proteinogenic amino acids, suggesting that the reduced availability of amino acids affect protein synthesis and consequently cell proliferation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Centro de Citricultura “Sylvio Moreira” – Instituto Agronômico de Campinas CordeirópolisDepartamento de Bioquímica e Microbiologia Instituto de Biociências Universidade Estadual PaulistaLaboratório Nacional de Ciência e Tecnologia do Bioetanol - Centro Nacional de Pesquisa em Energia e Materiais, CampinasMax-Planck-Institut Für Molekulare Pflanzenphysiologie Wissenschaftspark Golm, Am Mühlenberg 1Departamento de Bioquímica e Microbiologia Instituto de Biociências Universidade Estadual PaulistaFAPESP: 2013/01395-9FAPESP: 2013/10957-0FAPESP: 2014/50880-0FAPESP: 2018/10734-5CNPq: 465440/2014-2CordeirópolisUniversidade Estadual Paulista (UNESP)Laboratório Nacional de Ciência e Tecnologia do Bioetanol - Centro Nacional de Pesquisa em Energia e MateriaisWissenschaftspark GolmPicchi, Simone Cristinade Souza e Silva, MarianaSaldanha, Luiz Leonardo [UNESP]Ferreira, Henrique [UNESP]Takita, Marco AurélioCaldana, Camilade Souza, Alessandra Alves2022-05-01T08:15:18Z2022-05-01T08:15:18Z2021-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-021-95113-4Scientific Reports, v. 11, n. 1, 2021.2045-2322http://hdl.handle.net/11449/23340410.1038/s41598-021-95113-42-s2.0-85112661848Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2024-06-24T13:08:25Zoai:repositorio.unesp.br:11449/233404Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:54:11.419695Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
GC-TOF/MS-based metabolomics analysis to investigate the changes driven by N-Acetylcysteine in the plant-pathogen Xanthomonas citri subsp. citri |
title |
GC-TOF/MS-based metabolomics analysis to investigate the changes driven by N-Acetylcysteine in the plant-pathogen Xanthomonas citri subsp. citri |
spellingShingle |
GC-TOF/MS-based metabolomics analysis to investigate the changes driven by N-Acetylcysteine in the plant-pathogen Xanthomonas citri subsp. citri Picchi, Simone Cristina |
title_short |
GC-TOF/MS-based metabolomics analysis to investigate the changes driven by N-Acetylcysteine in the plant-pathogen Xanthomonas citri subsp. citri |
title_full |
GC-TOF/MS-based metabolomics analysis to investigate the changes driven by N-Acetylcysteine in the plant-pathogen Xanthomonas citri subsp. citri |
title_fullStr |
GC-TOF/MS-based metabolomics analysis to investigate the changes driven by N-Acetylcysteine in the plant-pathogen Xanthomonas citri subsp. citri |
title_full_unstemmed |
GC-TOF/MS-based metabolomics analysis to investigate the changes driven by N-Acetylcysteine in the plant-pathogen Xanthomonas citri subsp. citri |
title_sort |
GC-TOF/MS-based metabolomics analysis to investigate the changes driven by N-Acetylcysteine in the plant-pathogen Xanthomonas citri subsp. citri |
author |
Picchi, Simone Cristina |
author_facet |
Picchi, Simone Cristina de Souza e Silva, Mariana Saldanha, Luiz Leonardo [UNESP] Ferreira, Henrique [UNESP] Takita, Marco Aurélio Caldana, Camila de Souza, Alessandra Alves |
author_role |
author |
author2 |
de Souza e Silva, Mariana Saldanha, Luiz Leonardo [UNESP] Ferreira, Henrique [UNESP] Takita, Marco Aurélio Caldana, Camila de Souza, Alessandra Alves |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Cordeirópolis Universidade Estadual Paulista (UNESP) Laboratório Nacional de Ciência e Tecnologia do Bioetanol - Centro Nacional de Pesquisa em Energia e Materiais Wissenschaftspark Golm |
dc.contributor.author.fl_str_mv |
Picchi, Simone Cristina de Souza e Silva, Mariana Saldanha, Luiz Leonardo [UNESP] Ferreira, Henrique [UNESP] Takita, Marco Aurélio Caldana, Camila de Souza, Alessandra Alves |
description |
N-Acetylcysteine (NAC) is an antioxidant, anti-adhesive, and antimicrobial compound. Even though there is much information regarding the role of NAC as an antioxidant and anti-adhesive agent, little is known about its antimicrobial activity. In order to assess its mode of action in bacterial cells, we investigated the metabolic responses triggered by NAC at neutral pH. As a model organism, we chose the Gram-negative plant pathogen Xanthomonas citri subsp. citri (X. citri), the causal agent of citrus canker disease, due to the potential use of NAC as a sustainable molecule against phytopathogens dissemination in citrus cultivated areas. In presence of NAC, cell proliferation was affected after 4 h, but damages to the cell membrane were observed only after 24 h. Targeted metabolite profiling analysis using GC–MS/TOF unravelled that NAC seems to be metabolized by the cells affecting cysteine metabolism. Intriguingly, glutamine, a marker for nitrogen status, was not detected among the cells treated with NAC. The absence of glutamine was followed by a decrease in the levels of the majority of the proteinogenic amino acids, suggesting that the reduced availability of amino acids affect protein synthesis and consequently cell proliferation. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-01 2022-05-01T08:15:18Z 2022-05-01T08:15:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-021-95113-4 Scientific Reports, v. 11, n. 1, 2021. 2045-2322 http://hdl.handle.net/11449/233404 10.1038/s41598-021-95113-4 2-s2.0-85112661848 |
url |
http://dx.doi.org/10.1038/s41598-021-95113-4 http://hdl.handle.net/11449/233404 |
identifier_str_mv |
Scientific Reports, v. 11, n. 1, 2021. 2045-2322 10.1038/s41598-021-95113-4 2-s2.0-85112661848 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129471815876608 |