Analytical profile and antioxidant and anti-inflammatory activities of the enriched polyphenol fractions isolated from bergamot fruit and leave
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/antiox10020141 http://hdl.handle.net/11449/205764 |
Resumo: | The aim of the study is to compare the qualitative and semi-quantitative profile of the polyphenol fraction purified from the leaf (BLPF) and fruit (BFPF) of bergamot (Citrus bergamia), and to evaluate their antioxidant and anti-inflammatory activity. The analytical qualitative profile was carried out by LC-ESI/MS using three different approaches: targeted (searching analytes al-ready reported in bergamot extract), semi-targeted (a selective search of 3-hydroxy-3-methylglu-tarate [HMG] derivatives involved in the cholesterol reducing activity of BPF) and untargeted. A total number of 108 compounds were identified by using the three approaches, 100 of which are present in both the extracts thus demonstrating a good qualitative overlapping of polyphenols between the two extracts. The antioxidant activity was higher for BLPF in respect to BFPF but when normalized in respect to the polyphenol content they were almost overlapping. Both the extracts were found to dose dependently inhibit cell inflammation stimulated with IL-1α. In conclusion, the comparison of the qualitative and quantitative profile of polyphenols as well as of the antioxidant and anti-inflammatory activity of bergamot leaf and fruit well indicates that leaf is a valid source of bergamot polyphenol extraction and an even richer source of polyphenol in respect to the fruit. |
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Analytical profile and antioxidant and anti-inflammatory activities of the enriched polyphenol fractions isolated from bergamot fruit and leaveAntioxidantCholesterolCitrus bergamiaHigh-resolution mass spectrometryHMGInflam-mationMetabolic syndromePolyphenolsThe aim of the study is to compare the qualitative and semi-quantitative profile of the polyphenol fraction purified from the leaf (BLPF) and fruit (BFPF) of bergamot (Citrus bergamia), and to evaluate their antioxidant and anti-inflammatory activity. The analytical qualitative profile was carried out by LC-ESI/MS using three different approaches: targeted (searching analytes al-ready reported in bergamot extract), semi-targeted (a selective search of 3-hydroxy-3-methylglu-tarate [HMG] derivatives involved in the cholesterol reducing activity of BPF) and untargeted. A total number of 108 compounds were identified by using the three approaches, 100 of which are present in both the extracts thus demonstrating a good qualitative overlapping of polyphenols between the two extracts. The antioxidant activity was higher for BLPF in respect to BFPF but when normalized in respect to the polyphenol content they were almost overlapping. Both the extracts were found to dose dependently inhibit cell inflammation stimulated with IL-1α. In conclusion, the comparison of the qualitative and quantitative profile of polyphenols as well as of the antioxidant and anti-inflammatory activity of bergamot leaf and fruit well indicates that leaf is a valid source of bergamot polyphenol extraction and an even richer source of polyphenol in respect to the fruit.Department of Pharmaceutical Sciences University of MilanMedical School Sao Paulo State University (Unesp)Experimental Cardio-oncology and Cardiovascular Aging Unit Centro Cardiologico Monzino-IRCCS, Via Carlo Parea, 4H&AD SrlDepartment of Health Sciences University “Magna Graecia” of CatanzaroPlantexresearch SrlMedical School Sao Paulo State University (Unesp)University of MilanUniversidade Estadual Paulista (Unesp)Centro Cardiologico Monzino-IRCCSH&AD SrlUniversity “Magna Graecia” of CatanzaroPlantexresearch SrlBaron, GiovannaAltomare, AlessandraMol, MarcoGarcia, Jessica Leite [UNESP]Correa, Camila [UNESP]Raucci, AngelaMancinelli, LuigiMazzotta, SarahFumagalli, LauraTrunfio, GiuseppeTucci, LuigiLombardo, ElenaMalara, DomenicoJanda, ElzbietaMollace, VincenzoCarini, MarinaBombardelli, EzioAldini, Giancarlo2021-06-25T10:20:49Z2021-06-25T10:20:49Z2021-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-27http://dx.doi.org/10.3390/antiox10020141Antioxidants, v. 10, n. 2, p. 1-27, 2021.2076-3921http://hdl.handle.net/11449/20576410.3390/antiox100201412-s2.0-85099655375Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAntioxidantsinfo:eu-repo/semantics/openAccess2021-10-22T17:11:17Zoai:repositorio.unesp.br:11449/205764Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:58:53.719719Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Analytical profile and antioxidant and anti-inflammatory activities of the enriched polyphenol fractions isolated from bergamot fruit and leave |
title |
Analytical profile and antioxidant and anti-inflammatory activities of the enriched polyphenol fractions isolated from bergamot fruit and leave |
spellingShingle |
Analytical profile and antioxidant and anti-inflammatory activities of the enriched polyphenol fractions isolated from bergamot fruit and leave Baron, Giovanna Antioxidant Cholesterol Citrus bergamia High-resolution mass spectrometry HMG Inflam-mation Metabolic syndrome Polyphenols |
title_short |
Analytical profile and antioxidant and anti-inflammatory activities of the enriched polyphenol fractions isolated from bergamot fruit and leave |
title_full |
Analytical profile and antioxidant and anti-inflammatory activities of the enriched polyphenol fractions isolated from bergamot fruit and leave |
title_fullStr |
Analytical profile and antioxidant and anti-inflammatory activities of the enriched polyphenol fractions isolated from bergamot fruit and leave |
title_full_unstemmed |
Analytical profile and antioxidant and anti-inflammatory activities of the enriched polyphenol fractions isolated from bergamot fruit and leave |
title_sort |
Analytical profile and antioxidant and anti-inflammatory activities of the enriched polyphenol fractions isolated from bergamot fruit and leave |
author |
Baron, Giovanna |
author_facet |
Baron, Giovanna Altomare, Alessandra Mol, Marco Garcia, Jessica Leite [UNESP] Correa, Camila [UNESP] Raucci, Angela Mancinelli, Luigi Mazzotta, Sarah Fumagalli, Laura Trunfio, Giuseppe Tucci, Luigi Lombardo, Elena Malara, Domenico Janda, Elzbieta Mollace, Vincenzo Carini, Marina Bombardelli, Ezio Aldini, Giancarlo |
author_role |
author |
author2 |
Altomare, Alessandra Mol, Marco Garcia, Jessica Leite [UNESP] Correa, Camila [UNESP] Raucci, Angela Mancinelli, Luigi Mazzotta, Sarah Fumagalli, Laura Trunfio, Giuseppe Tucci, Luigi Lombardo, Elena Malara, Domenico Janda, Elzbieta Mollace, Vincenzo Carini, Marina Bombardelli, Ezio Aldini, Giancarlo |
author2_role |
author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Milan Universidade Estadual Paulista (Unesp) Centro Cardiologico Monzino-IRCCS H&AD Srl University “Magna Graecia” of Catanzaro Plantexresearch Srl |
dc.contributor.author.fl_str_mv |
Baron, Giovanna Altomare, Alessandra Mol, Marco Garcia, Jessica Leite [UNESP] Correa, Camila [UNESP] Raucci, Angela Mancinelli, Luigi Mazzotta, Sarah Fumagalli, Laura Trunfio, Giuseppe Tucci, Luigi Lombardo, Elena Malara, Domenico Janda, Elzbieta Mollace, Vincenzo Carini, Marina Bombardelli, Ezio Aldini, Giancarlo |
dc.subject.por.fl_str_mv |
Antioxidant Cholesterol Citrus bergamia High-resolution mass spectrometry HMG Inflam-mation Metabolic syndrome Polyphenols |
topic |
Antioxidant Cholesterol Citrus bergamia High-resolution mass spectrometry HMG Inflam-mation Metabolic syndrome Polyphenols |
description |
The aim of the study is to compare the qualitative and semi-quantitative profile of the polyphenol fraction purified from the leaf (BLPF) and fruit (BFPF) of bergamot (Citrus bergamia), and to evaluate their antioxidant and anti-inflammatory activity. The analytical qualitative profile was carried out by LC-ESI/MS using three different approaches: targeted (searching analytes al-ready reported in bergamot extract), semi-targeted (a selective search of 3-hydroxy-3-methylglu-tarate [HMG] derivatives involved in the cholesterol reducing activity of BPF) and untargeted. A total number of 108 compounds were identified by using the three approaches, 100 of which are present in both the extracts thus demonstrating a good qualitative overlapping of polyphenols between the two extracts. The antioxidant activity was higher for BLPF in respect to BFPF but when normalized in respect to the polyphenol content they were almost overlapping. Both the extracts were found to dose dependently inhibit cell inflammation stimulated with IL-1α. In conclusion, the comparison of the qualitative and quantitative profile of polyphenols as well as of the antioxidant and anti-inflammatory activity of bergamot leaf and fruit well indicates that leaf is a valid source of bergamot polyphenol extraction and an even richer source of polyphenol in respect to the fruit. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:20:49Z 2021-06-25T10:20:49Z 2021-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/antiox10020141 Antioxidants, v. 10, n. 2, p. 1-27, 2021. 2076-3921 http://hdl.handle.net/11449/205764 10.3390/antiox10020141 2-s2.0-85099655375 |
url |
http://dx.doi.org/10.3390/antiox10020141 http://hdl.handle.net/11449/205764 |
identifier_str_mv |
Antioxidants, v. 10, n. 2, p. 1-27, 2021. 2076-3921 10.3390/antiox10020141 2-s2.0-85099655375 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Antioxidants |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-27 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128300343623680 |