Micro-Fourier-transform infrared reflectance spectroscopy as tool for probing IgG glycosylation in COVID-19 patients

Detalhes bibliográficos
Autor(a) principal: Bandeira, Carla Carolina Silva
Data de Publicação: 2022
Outros Autores: Madureira, Karen Cristina Rolim, Rossi, Meire Bocoli, Gallo, Juliana Failde, da Silva, Ana Paula Marques Aguirra, Torres, Vilanilse Lopes, de Lima, Vinicius Alves, Júnior, Norival Kesper, Almeida, Janete Dias [UNESP], Zerbinati, Rodrigo Melim, Braz-Silva, Paulo Henrique, Lindoso, José Angelo Lauletta, da Silva Martinho, Herculano
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1038/s41598-022-08156-6
http://hdl.handle.net/11449/230560
Resumo: It has been reported that patients diagnosed with COVID-19 become critically ill primarily around the time of activation of the adaptive immune response. However the role of antibodies in the worsening of disease is not obvious. Higher titers of anti-spike immunoglobulin IgG1 associated with low fucosylation of the antibody Fc tail have been associated to excessive inflammatory response. In contrast it has been also reported that NP-, S-, RBD- specific IgA, IgG, and IgM are not associated with SARS-CoV-2 viral load, indicating that there is no obvious correlation between antibody response and viral antigen detection. In the present work the micro-Fourier-transform infrared reflectance spectroscopy (micro-FTIR) was employed to investigate blood serum samples of healthy and COVID-19-ill (mild or oligosymptomatic) individuals (82 healthcare workers volunteers in “Instituto de Infectologia Emilio Ribas”, São Paulo, Brazil). The molecular-level-sensitive, multiplexing quantitative and qualitative FTIR data probed on 1 µL of dried biofluid was compared to signal-to-cutoff index of chemiluminescent immunoassays CLIA and ELISA (IgG antibodies against SARS-CoV-2). Our main result indicated that 1702–1785 cm - 1 spectral window (carbonyl C=O vibration) is a spectral marker of the degree of IgG glycosylation, allowing to probe distinctive sub-populations of COVID-19 patients, depending on their degree of severity. The specificity was 87.5 % while the detection rate of true positive was 100%. The computed area under the receiver operating curve was equivalent to CLIA, ELISA and other ATR-FTIR methods (> 0.85). In summary, overall discrimination of healthy and COVID-19 individuals and severity prediction as well could be potentially implemented using micro-FTIR reflectance spectroscopy on blood serum samples. Considering the minimal and reagent-free sample preparation procedures combined to fast (few minutes) outcome of FTIR we can state that this technology is suitable for fast screening of immune response of individuals with COVID-19. It would be an important tool in prospective studies, helping investigate the physiology of the asymptomatic, oligosymptomatic, or severe individuals and measure the extension of infection dissemination in patients.
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spelling Micro-Fourier-transform infrared reflectance spectroscopy as tool for probing IgG glycosylation in COVID-19 patientsIt has been reported that patients diagnosed with COVID-19 become critically ill primarily around the time of activation of the adaptive immune response. However the role of antibodies in the worsening of disease is not obvious. Higher titers of anti-spike immunoglobulin IgG1 associated with low fucosylation of the antibody Fc tail have been associated to excessive inflammatory response. In contrast it has been also reported that NP-, S-, RBD- specific IgA, IgG, and IgM are not associated with SARS-CoV-2 viral load, indicating that there is no obvious correlation between antibody response and viral antigen detection. In the present work the micro-Fourier-transform infrared reflectance spectroscopy (micro-FTIR) was employed to investigate blood serum samples of healthy and COVID-19-ill (mild or oligosymptomatic) individuals (82 healthcare workers volunteers in “Instituto de Infectologia Emilio Ribas”, São Paulo, Brazil). The molecular-level-sensitive, multiplexing quantitative and qualitative FTIR data probed on 1 µL of dried biofluid was compared to signal-to-cutoff index of chemiluminescent immunoassays CLIA and ELISA (IgG antibodies against SARS-CoV-2). Our main result indicated that 1702–1785 cm - 1 spectral window (carbonyl C=O vibration) is a spectral marker of the degree of IgG glycosylation, allowing to probe distinctive sub-populations of COVID-19 patients, depending on their degree of severity. The specificity was 87.5 % while the detection rate of true positive was 100%. The computed area under the receiver operating curve was equivalent to CLIA, ELISA and other ATR-FTIR methods (> 0.85). In summary, overall discrimination of healthy and COVID-19 individuals and severity prediction as well could be potentially implemented using micro-FTIR reflectance spectroscopy on blood serum samples. Considering the minimal and reagent-free sample preparation procedures combined to fast (few minutes) outcome of FTIR we can state that this technology is suitable for fast screening of immune response of individuals with COVID-19. It would be an important tool in prospective studies, helping investigate the physiology of the asymptomatic, oligosymptomatic, or severe individuals and measure the extension of infection dissemination in patients.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Centro de Ciências Naturais e Humanas Universidade Federal do ABC, SPInstituto de Infectologia Emilio Ribas, SpInstituto de Medicina Tropical de São Paulo Universidade de São Paulo, SPDepartamento de Biociências e Diagnêstico Instituto de Ciência e Tecnologia Universidade Estadual Paulista, SPFaculdade de Odontologia Departamento de Estomatologia Universidade de São Paulo, SPDepartamento de Moléstias Infecciosas e Parasitárias Faculdade de Medicina Universidade de São Paulo, SPDepartamento de Biociências e Diagnêstico Instituto de Ciência e Tecnologia Universidade Estadual Paulista, SPFAPESP: 2011/19924-2CNPq: 307718/2019-0CNPq: 311146/2015-5Universidade Federal do ABC (UFABC)Instituto de Infectologia Emilio RibasUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Bandeira, Carla Carolina SilvaMadureira, Karen Cristina RolimRossi, Meire BocoliGallo, Juliana Faildeda Silva, Ana Paula Marques AguirraTorres, Vilanilse Lopesde Lima, Vinicius AlvesJúnior, Norival KesperAlmeida, Janete Dias [UNESP]Zerbinati, Rodrigo MelimBraz-Silva, Paulo HenriqueLindoso, José Angelo Laulettada Silva Martinho, Herculano2022-04-29T08:40:46Z2022-04-29T08:40:46Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-022-08156-6Scientific Reports, v. 12, n. 1, 2022.2045-2322http://hdl.handle.net/11449/23056010.1038/s41598-022-08156-62-s2.0-85126203561Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2022-04-29T08:40:46Zoai:repositorio.unesp.br:11449/230560Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:46:05.029757Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Micro-Fourier-transform infrared reflectance spectroscopy as tool for probing IgG glycosylation in COVID-19 patients
title Micro-Fourier-transform infrared reflectance spectroscopy as tool for probing IgG glycosylation in COVID-19 patients
spellingShingle Micro-Fourier-transform infrared reflectance spectroscopy as tool for probing IgG glycosylation in COVID-19 patients
Bandeira, Carla Carolina Silva
title_short Micro-Fourier-transform infrared reflectance spectroscopy as tool for probing IgG glycosylation in COVID-19 patients
title_full Micro-Fourier-transform infrared reflectance spectroscopy as tool for probing IgG glycosylation in COVID-19 patients
title_fullStr Micro-Fourier-transform infrared reflectance spectroscopy as tool for probing IgG glycosylation in COVID-19 patients
title_full_unstemmed Micro-Fourier-transform infrared reflectance spectroscopy as tool for probing IgG glycosylation in COVID-19 patients
title_sort Micro-Fourier-transform infrared reflectance spectroscopy as tool for probing IgG glycosylation in COVID-19 patients
author Bandeira, Carla Carolina Silva
author_facet Bandeira, Carla Carolina Silva
Madureira, Karen Cristina Rolim
Rossi, Meire Bocoli
Gallo, Juliana Failde
da Silva, Ana Paula Marques Aguirra
Torres, Vilanilse Lopes
de Lima, Vinicius Alves
Júnior, Norival Kesper
Almeida, Janete Dias [UNESP]
Zerbinati, Rodrigo Melim
Braz-Silva, Paulo Henrique
Lindoso, José Angelo Lauletta
da Silva Martinho, Herculano
author_role author
author2 Madureira, Karen Cristina Rolim
Rossi, Meire Bocoli
Gallo, Juliana Failde
da Silva, Ana Paula Marques Aguirra
Torres, Vilanilse Lopes
de Lima, Vinicius Alves
Júnior, Norival Kesper
Almeida, Janete Dias [UNESP]
Zerbinati, Rodrigo Melim
Braz-Silva, Paulo Henrique
Lindoso, José Angelo Lauletta
da Silva Martinho, Herculano
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal do ABC (UFABC)
Instituto de Infectologia Emilio Ribas
Universidade de São Paulo (USP)
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Bandeira, Carla Carolina Silva
Madureira, Karen Cristina Rolim
Rossi, Meire Bocoli
Gallo, Juliana Failde
da Silva, Ana Paula Marques Aguirra
Torres, Vilanilse Lopes
de Lima, Vinicius Alves
Júnior, Norival Kesper
Almeida, Janete Dias [UNESP]
Zerbinati, Rodrigo Melim
Braz-Silva, Paulo Henrique
Lindoso, José Angelo Lauletta
da Silva Martinho, Herculano
description It has been reported that patients diagnosed with COVID-19 become critically ill primarily around the time of activation of the adaptive immune response. However the role of antibodies in the worsening of disease is not obvious. Higher titers of anti-spike immunoglobulin IgG1 associated with low fucosylation of the antibody Fc tail have been associated to excessive inflammatory response. In contrast it has been also reported that NP-, S-, RBD- specific IgA, IgG, and IgM are not associated with SARS-CoV-2 viral load, indicating that there is no obvious correlation between antibody response and viral antigen detection. In the present work the micro-Fourier-transform infrared reflectance spectroscopy (micro-FTIR) was employed to investigate blood serum samples of healthy and COVID-19-ill (mild or oligosymptomatic) individuals (82 healthcare workers volunteers in “Instituto de Infectologia Emilio Ribas”, São Paulo, Brazil). The molecular-level-sensitive, multiplexing quantitative and qualitative FTIR data probed on 1 µL of dried biofluid was compared to signal-to-cutoff index of chemiluminescent immunoassays CLIA and ELISA (IgG antibodies against SARS-CoV-2). Our main result indicated that 1702–1785 cm - 1 spectral window (carbonyl C=O vibration) is a spectral marker of the degree of IgG glycosylation, allowing to probe distinctive sub-populations of COVID-19 patients, depending on their degree of severity. The specificity was 87.5 % while the detection rate of true positive was 100%. The computed area under the receiver operating curve was equivalent to CLIA, ELISA and other ATR-FTIR methods (> 0.85). In summary, overall discrimination of healthy and COVID-19 individuals and severity prediction as well could be potentially implemented using micro-FTIR reflectance spectroscopy on blood serum samples. Considering the minimal and reagent-free sample preparation procedures combined to fast (few minutes) outcome of FTIR we can state that this technology is suitable for fast screening of immune response of individuals with COVID-19. It would be an important tool in prospective studies, helping investigate the physiology of the asymptomatic, oligosymptomatic, or severe individuals and measure the extension of infection dissemination in patients.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-29T08:40:46Z
2022-04-29T08:40:46Z
2022-12-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1038/s41598-022-08156-6
Scientific Reports, v. 12, n. 1, 2022.
2045-2322
http://hdl.handle.net/11449/230560
10.1038/s41598-022-08156-6
2-s2.0-85126203561
url http://dx.doi.org/10.1038/s41598-022-08156-6
http://hdl.handle.net/11449/230560
identifier_str_mv Scientific Reports, v. 12, n. 1, 2022.
2045-2322
10.1038/s41598-022-08156-6
2-s2.0-85126203561
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Scientific Reports
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
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institution UNESP
reponame_str Repositório Institucional da UNESP
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repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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