PBMT and topical diclofenac as single and combined treatment on skeletal muscle injury in diabetic rats: effects on biochemical and functional aspects
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10103-018-2580-z http://hdl.handle.net/11449/171200 |
Resumo: | Physical exercise generates several benefits in a short time in patients with diabetes mellitus. However, it can increase the chances of muscle damage, a serious problem for diabetic patients. Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used to treat these injuries, despite the serious adverse effects. In this way, photobiomodulation therapy (PBMT) with low-level laser therapy (LLLT) and/or light emitting diode therapy (LEDT) can be used as an alternative in this case. However, its efficacy in tissue repair of trauma injuries in diabetes mellitus until now is unknown, as well as the combination between PBMT and NSAIDs. The objective of the present study was to evaluate the effects of NSAIDs and PBMT applied alone or combined on functional and biochemical aspects, in an experimental model of muscle injury through controlled trauma in diabetic rats. Muscle injury was induced by means of a single trauma to the animals’ anterior tibialis muscle. After 1 h, the rats were treated with PBMT (830 nm; continuous mode, with a power output of 100 mW; 3.57 W/cm2; 3 J; 107.1 J/cm2, 30 s), diclofenac sodium for topical use (1 g), or combination of them. Our results demonstrated that PBMT + diclofenac, and PBMT alone reduced the gene expression of cyclooxygenase-2 (COX-2) at all assessed times as compared to the injury and diclofenac groups (p < 0.05 and p < 0.01 respectively). The diclofenac alone showed reduced levels of COX-2 only in relation to the injury group (p < 0.05). Prostaglandin E2 levels in blood plasma demonstrated similar results to COX2. In addition, we observed that PBMT + diclofenac and PBMT alone showed significant improvement compared with injury and diclofenac groups in functional analysis at all time points. The results indicate that PBMT alone or in combination with diclofenac reduces levels of inflammatory markers and improves gait of diabetic rats in the acute phase of muscle injury. |
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PBMT and topical diclofenac as single and combined treatment on skeletal muscle injury in diabetic rats: effects on biochemical and functional aspectsDiabetes mellitusLow-level laser therapyPhotobiomodulation therapyRatsSkeletal muscle injuryTopical nonsteroidal anti-inflammatory drugPhysical exercise generates several benefits in a short time in patients with diabetes mellitus. However, it can increase the chances of muscle damage, a serious problem for diabetic patients. Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used to treat these injuries, despite the serious adverse effects. In this way, photobiomodulation therapy (PBMT) with low-level laser therapy (LLLT) and/or light emitting diode therapy (LEDT) can be used as an alternative in this case. However, its efficacy in tissue repair of trauma injuries in diabetes mellitus until now is unknown, as well as the combination between PBMT and NSAIDs. The objective of the present study was to evaluate the effects of NSAIDs and PBMT applied alone or combined on functional and biochemical aspects, in an experimental model of muscle injury through controlled trauma in diabetic rats. Muscle injury was induced by means of a single trauma to the animals’ anterior tibialis muscle. After 1 h, the rats were treated with PBMT (830 nm; continuous mode, with a power output of 100 mW; 3.57 W/cm2; 3 J; 107.1 J/cm2, 30 s), diclofenac sodium for topical use (1 g), or combination of them. Our results demonstrated that PBMT + diclofenac, and PBMT alone reduced the gene expression of cyclooxygenase-2 (COX-2) at all assessed times as compared to the injury and diclofenac groups (p < 0.05 and p < 0.01 respectively). The diclofenac alone showed reduced levels of COX-2 only in relation to the injury group (p < 0.05). Prostaglandin E2 levels in blood plasma demonstrated similar results to COX2. In addition, we observed that PBMT + diclofenac and PBMT alone showed significant improvement compared with injury and diclofenac groups in functional analysis at all time points. The results indicate that PBMT alone or in combination with diclofenac reduces levels of inflammatory markers and improves gait of diabetic rats in the acute phase of muscle injury.Masters in Physical Therapy Universidade do Sagrado CoraçãoGraduation in Nutrition Universidade do Sagrado CoraçãoPostgraduate Program in Biophotonics Applied to Health Sciences Nove de Julho University (UNINOVE)Laboratório de Biofotônica e Terapêutica Experimental Instituto de Pesquisa e Desenvolvimento Universidade do Vale do ParaíbaMasters and Doctoral Programs in Physical Therapy Universidade Cidade de São Paulo (UNICID)Laboratory of Phototherapy in Sport and Exercise Nove de Julho University (UNINOVE)Postgraduate Program in Rehabilitation Sciences Nove de Julho University (UNINOVE)Laboratory of Physiology and Sport Performance (LAFIDE) School of Sciences São Paulo State University - UNESP, Av. Eng. Luiz Edmundo C. CoubeLaboratory of Physiology and Sport Performance (LAFIDE) School of Sciences São Paulo State University - UNESP, Av. Eng. Luiz Edmundo C. CoubeUniversidade do Sagrado CoraçãoNove de Julho University (UNINOVE)Universidade do Vale do ParaíbaUniversidade Cidade de São Paulo (UNICID)Universidade Estadual Paulista (Unesp)Dos Santos, Ligiane SouzaSaltorato, Joyce CamillaMonte, Marina GaiatoMarcos, Rodrigo LabatLopes-Martins, Rodrigo Álvaro BrandãoTomazoni, Shaiane SilvaLeal-Junior, Ernesto Cesar Pintode Paiva Carvalho, Rodrigo Leal [UNESP]2018-12-11T16:54:22Z2018-12-11T16:54:22Z2018-07-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-8application/pdfhttp://dx.doi.org/10.1007/s10103-018-2580-zLasers in Medical Science, p. 1-8.1435-604X0268-8921http://hdl.handle.net/11449/17120010.1007/s10103-018-2580-z2-s2.0-850496853602-s2.0-85049685360.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLasers in Medical Science0,713info:eu-repo/semantics/openAccess2023-11-08T06:14:05Zoai:repositorio.unesp.br:11449/171200Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:10:57.206596Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
PBMT and topical diclofenac as single and combined treatment on skeletal muscle injury in diabetic rats: effects on biochemical and functional aspects |
title |
PBMT and topical diclofenac as single and combined treatment on skeletal muscle injury in diabetic rats: effects on biochemical and functional aspects |
spellingShingle |
PBMT and topical diclofenac as single and combined treatment on skeletal muscle injury in diabetic rats: effects on biochemical and functional aspects Dos Santos, Ligiane Souza Diabetes mellitus Low-level laser therapy Photobiomodulation therapy Rats Skeletal muscle injury Topical nonsteroidal anti-inflammatory drug |
title_short |
PBMT and topical diclofenac as single and combined treatment on skeletal muscle injury in diabetic rats: effects on biochemical and functional aspects |
title_full |
PBMT and topical diclofenac as single and combined treatment on skeletal muscle injury in diabetic rats: effects on biochemical and functional aspects |
title_fullStr |
PBMT and topical diclofenac as single and combined treatment on skeletal muscle injury in diabetic rats: effects on biochemical and functional aspects |
title_full_unstemmed |
PBMT and topical diclofenac as single and combined treatment on skeletal muscle injury in diabetic rats: effects on biochemical and functional aspects |
title_sort |
PBMT and topical diclofenac as single and combined treatment on skeletal muscle injury in diabetic rats: effects on biochemical and functional aspects |
author |
Dos Santos, Ligiane Souza |
author_facet |
Dos Santos, Ligiane Souza Saltorato, Joyce Camilla Monte, Marina Gaiato Marcos, Rodrigo Labat Lopes-Martins, Rodrigo Álvaro Brandão Tomazoni, Shaiane Silva Leal-Junior, Ernesto Cesar Pinto de Paiva Carvalho, Rodrigo Leal [UNESP] |
author_role |
author |
author2 |
Saltorato, Joyce Camilla Monte, Marina Gaiato Marcos, Rodrigo Labat Lopes-Martins, Rodrigo Álvaro Brandão Tomazoni, Shaiane Silva Leal-Junior, Ernesto Cesar Pinto de Paiva Carvalho, Rodrigo Leal [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Sagrado Coração Nove de Julho University (UNINOVE) Universidade do Vale do Paraíba Universidade Cidade de São Paulo (UNICID) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Dos Santos, Ligiane Souza Saltorato, Joyce Camilla Monte, Marina Gaiato Marcos, Rodrigo Labat Lopes-Martins, Rodrigo Álvaro Brandão Tomazoni, Shaiane Silva Leal-Junior, Ernesto Cesar Pinto de Paiva Carvalho, Rodrigo Leal [UNESP] |
dc.subject.por.fl_str_mv |
Diabetes mellitus Low-level laser therapy Photobiomodulation therapy Rats Skeletal muscle injury Topical nonsteroidal anti-inflammatory drug |
topic |
Diabetes mellitus Low-level laser therapy Photobiomodulation therapy Rats Skeletal muscle injury Topical nonsteroidal anti-inflammatory drug |
description |
Physical exercise generates several benefits in a short time in patients with diabetes mellitus. However, it can increase the chances of muscle damage, a serious problem for diabetic patients. Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used to treat these injuries, despite the serious adverse effects. In this way, photobiomodulation therapy (PBMT) with low-level laser therapy (LLLT) and/or light emitting diode therapy (LEDT) can be used as an alternative in this case. However, its efficacy in tissue repair of trauma injuries in diabetes mellitus until now is unknown, as well as the combination between PBMT and NSAIDs. The objective of the present study was to evaluate the effects of NSAIDs and PBMT applied alone or combined on functional and biochemical aspects, in an experimental model of muscle injury through controlled trauma in diabetic rats. Muscle injury was induced by means of a single trauma to the animals’ anterior tibialis muscle. After 1 h, the rats were treated with PBMT (830 nm; continuous mode, with a power output of 100 mW; 3.57 W/cm2; 3 J; 107.1 J/cm2, 30 s), diclofenac sodium for topical use (1 g), or combination of them. Our results demonstrated that PBMT + diclofenac, and PBMT alone reduced the gene expression of cyclooxygenase-2 (COX-2) at all assessed times as compared to the injury and diclofenac groups (p < 0.05 and p < 0.01 respectively). The diclofenac alone showed reduced levels of COX-2 only in relation to the injury group (p < 0.05). Prostaglandin E2 levels in blood plasma demonstrated similar results to COX2. In addition, we observed that PBMT + diclofenac and PBMT alone showed significant improvement compared with injury and diclofenac groups in functional analysis at all time points. The results indicate that PBMT alone or in combination with diclofenac reduces levels of inflammatory markers and improves gait of diabetic rats in the acute phase of muscle injury. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T16:54:22Z 2018-12-11T16:54:22Z 2018-07-10 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10103-018-2580-z Lasers in Medical Science, p. 1-8. 1435-604X 0268-8921 http://hdl.handle.net/11449/171200 10.1007/s10103-018-2580-z 2-s2.0-85049685360 2-s2.0-85049685360.pdf |
url |
http://dx.doi.org/10.1007/s10103-018-2580-z http://hdl.handle.net/11449/171200 |
identifier_str_mv |
Lasers in Medical Science, p. 1-8. 1435-604X 0268-8921 10.1007/s10103-018-2580-z 2-s2.0-85049685360 2-s2.0-85049685360.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lasers in Medical Science 0,713 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-8 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128767897370624 |