Identification of phospholipase C zeta in normospermic and teratospermic domestic cat sperm
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.theriogenology.2013.06.005 http://hdl.handle.net/11449/76853 |
Resumo: | In mammalian species, oocyte activation is initiated by oscillations in the intracellular concentration of free calcium ([Ca2+]i), which are also essential to allow embryonic development. To date, evidence supporting the hypothesis that a sperm factor is responsible for initiating oocyte activation has been presented in various mammalian species. Among the possible candidates to be the active sperm factor is the novel sperm-specific phospholipase C ζ (PLCζ), which besides its testis-specific expression is capable of initiating [Ca2+]i oscillations. In this study, we investigated the presence of PLCζ in the sperm of the domestic cat and whether normospermic and teratospermic cats differ in their PLCζ expression. Immunoblotting with anti-PLCζ antibodies confirmed the presence of an immunoreactive band of ~70 kDa in whole sperm lysates of domestic cat as well as in both soluble and insoluble fractions from this sperm. Additional immunoreactive bands, probably C- and N-terminal truncated versions of PLCζ, were also visualized in the soluble sperm fractions. Interestingly, immunoreactivity of PLCζ was detectable in teratospermic sperm, although with slightly less intensity than in normospermic sperm. In conclusion, domestic cat sperm express PLCζ in both cytosolic and high-pH fractions, which is consistent with data in other mammals. Sperm from teratospermic cats also express PLCζ, albeit at reduced concentrations, which may affect the fertility of these males. © 2013 Elsevier Inc.. |
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Identification of phospholipase C zeta in normospermic and teratospermic domestic cat spermFertilizationImmunoblottingOocyte activationPLCζTwo-dimensional PAGEFelis catusMammaliaIn mammalian species, oocyte activation is initiated by oscillations in the intracellular concentration of free calcium ([Ca2+]i), which are also essential to allow embryonic development. To date, evidence supporting the hypothesis that a sperm factor is responsible for initiating oocyte activation has been presented in various mammalian species. Among the possible candidates to be the active sperm factor is the novel sperm-specific phospholipase C ζ (PLCζ), which besides its testis-specific expression is capable of initiating [Ca2+]i oscillations. In this study, we investigated the presence of PLCζ in the sperm of the domestic cat and whether normospermic and teratospermic cats differ in their PLCζ expression. Immunoblotting with anti-PLCζ antibodies confirmed the presence of an immunoreactive band of ~70 kDa in whole sperm lysates of domestic cat as well as in both soluble and insoluble fractions from this sperm. Additional immunoreactive bands, probably C- and N-terminal truncated versions of PLCζ, were also visualized in the soluble sperm fractions. Interestingly, immunoreactivity of PLCζ was detectable in teratospermic sperm, although with slightly less intensity than in normospermic sperm. In conclusion, domestic cat sperm express PLCζ in both cytosolic and high-pH fractions, which is consistent with data in other mammals. Sperm from teratospermic cats also express PLCζ, albeit at reduced concentrations, which may affect the fertility of these males. © 2013 Elsevier Inc..Department of Animal Reproduction and Veterinary Radiology College of Veterinary Medicine and Animal Science São Paulo State University, UNESP, Botucatu, São PauloDepartment of Veterinary and Animal Sciences University of Massachusetts, Amherst, MAUniversidade de Franca UNIFRAN, Franca, São PauloDepartment of Animal Reproduction and Veterinary Radiology College of Veterinary Medicine and Animal Science São Paulo State University, UNESP, Botucatu, São PauloUniversidade Estadual Paulista (Unesp)University of MassachusettsUNIFRANVillaverde, Ana Izabel S. Balbin [UNESP]Fioratti, Eduardo G. [UNESP]Fissore, Rafael A.He, ChangliLee, Hoi ChangSouza, Fabiana Ferreira de [UNESP]Landim-Alvarenga, Fernanda C. [UNESP]Lopes, Maria Denise [UNESP]2014-05-27T11:30:51Z2014-05-27T11:30:51Z2013-10-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article722-729application/pdfhttp://dx.doi.org/10.1016/j.theriogenology.2013.06.005Theriogenology, v. 80, n. 7, p. 722-729, 2013.0093-691Xhttp://hdl.handle.net/11449/7685310.1016/j.theriogenology.2013.06.005WOS:0003246607000042-s2.0-848837936982-s2.0-84883793698.pdf666612991466301884564903008148330000-0002-2420-2550Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTheriogenology2.136info:eu-repo/semantics/openAccess2024-09-09T14:06:04Zoai:repositorio.unesp.br:11449/76853Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:06:04Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Identification of phospholipase C zeta in normospermic and teratospermic domestic cat sperm |
title |
Identification of phospholipase C zeta in normospermic and teratospermic domestic cat sperm |
spellingShingle |
Identification of phospholipase C zeta in normospermic and teratospermic domestic cat sperm Villaverde, Ana Izabel S. Balbin [UNESP] Fertilization Immunoblotting Oocyte activation PLCζ Two-dimensional PAGE Felis catus Mammalia |
title_short |
Identification of phospholipase C zeta in normospermic and teratospermic domestic cat sperm |
title_full |
Identification of phospholipase C zeta in normospermic and teratospermic domestic cat sperm |
title_fullStr |
Identification of phospholipase C zeta in normospermic and teratospermic domestic cat sperm |
title_full_unstemmed |
Identification of phospholipase C zeta in normospermic and teratospermic domestic cat sperm |
title_sort |
Identification of phospholipase C zeta in normospermic and teratospermic domestic cat sperm |
author |
Villaverde, Ana Izabel S. Balbin [UNESP] |
author_facet |
Villaverde, Ana Izabel S. Balbin [UNESP] Fioratti, Eduardo G. [UNESP] Fissore, Rafael A. He, Changli Lee, Hoi Chang Souza, Fabiana Ferreira de [UNESP] Landim-Alvarenga, Fernanda C. [UNESP] Lopes, Maria Denise [UNESP] |
author_role |
author |
author2 |
Fioratti, Eduardo G. [UNESP] Fissore, Rafael A. He, Changli Lee, Hoi Chang Souza, Fabiana Ferreira de [UNESP] Landim-Alvarenga, Fernanda C. [UNESP] Lopes, Maria Denise [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Massachusetts UNIFRAN |
dc.contributor.author.fl_str_mv |
Villaverde, Ana Izabel S. Balbin [UNESP] Fioratti, Eduardo G. [UNESP] Fissore, Rafael A. He, Changli Lee, Hoi Chang Souza, Fabiana Ferreira de [UNESP] Landim-Alvarenga, Fernanda C. [UNESP] Lopes, Maria Denise [UNESP] |
dc.subject.por.fl_str_mv |
Fertilization Immunoblotting Oocyte activation PLCζ Two-dimensional PAGE Felis catus Mammalia |
topic |
Fertilization Immunoblotting Oocyte activation PLCζ Two-dimensional PAGE Felis catus Mammalia |
description |
In mammalian species, oocyte activation is initiated by oscillations in the intracellular concentration of free calcium ([Ca2+]i), which are also essential to allow embryonic development. To date, evidence supporting the hypothesis that a sperm factor is responsible for initiating oocyte activation has been presented in various mammalian species. Among the possible candidates to be the active sperm factor is the novel sperm-specific phospholipase C ζ (PLCζ), which besides its testis-specific expression is capable of initiating [Ca2+]i oscillations. In this study, we investigated the presence of PLCζ in the sperm of the domestic cat and whether normospermic and teratospermic cats differ in their PLCζ expression. Immunoblotting with anti-PLCζ antibodies confirmed the presence of an immunoreactive band of ~70 kDa in whole sperm lysates of domestic cat as well as in both soluble and insoluble fractions from this sperm. Additional immunoreactive bands, probably C- and N-terminal truncated versions of PLCζ, were also visualized in the soluble sperm fractions. Interestingly, immunoreactivity of PLCζ was detectable in teratospermic sperm, although with slightly less intensity than in normospermic sperm. In conclusion, domestic cat sperm express PLCζ in both cytosolic and high-pH fractions, which is consistent with data in other mammals. Sperm from teratospermic cats also express PLCζ, albeit at reduced concentrations, which may affect the fertility of these males. © 2013 Elsevier Inc.. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-10-15 2014-05-27T11:30:51Z 2014-05-27T11:30:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.theriogenology.2013.06.005 Theriogenology, v. 80, n. 7, p. 722-729, 2013. 0093-691X http://hdl.handle.net/11449/76853 10.1016/j.theriogenology.2013.06.005 WOS:000324660700004 2-s2.0-84883793698 2-s2.0-84883793698.pdf 6666129914663018 8456490300814833 0000-0002-2420-2550 |
url |
http://dx.doi.org/10.1016/j.theriogenology.2013.06.005 http://hdl.handle.net/11449/76853 |
identifier_str_mv |
Theriogenology, v. 80, n. 7, p. 722-729, 2013. 0093-691X 10.1016/j.theriogenology.2013.06.005 WOS:000324660700004 2-s2.0-84883793698 2-s2.0-84883793698.pdf 6666129914663018 8456490300814833 0000-0002-2420-2550 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Theriogenology 2.136 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
722-729 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021345835089920 |