Genotypic diversity among p. Parasitica isolates reveled by its-5.8s rdna nucleotide sequences.

Detalhes bibliográficos
Autor(a) principal: Rosa, Daniel Dias
Data de Publicação: 2006
Outros Autores: Machado, Marcos Antonio, Targon, Maria Luisa Penteado Natividade, Furtado, Edson Luiz [UNESP]
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/228204
Resumo: Phytophthora parasitica is been considered one of the most prevalent citrus soilborn pathogens in Brazil. Fourteen isolates were collected in different region of São Paulo state. Amplification of the ITS region using primers ITS1 and ITS2 produced DNA fragments of 812 to 860 bp, that were compared with other sequences obtained to Gene Bank (NCBI). Neighbor-joining analysis, with 1000 bootstrap revealed an average similarity of 99,3 to 100% between the isolates, and 98,1 to 99,9% between S. Paulo state isolates and P. nicotianae (= Phytophthora parasitica) obtained from the Gene Bank (gi|8927482). The ITS1 was more conserved than to the ITS2. This is a good tool for quick diagnosis of Phytophthora citrus dise-ases.
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spelling Genotypic diversity among p. Parasitica isolates reveled by its-5.8s rdna nucleotide sequences.Diversidade de phytophthora parasitica isolados de citrus usando seqüências de nucleotídeos da região its-5.8s rdnaCitrus gummosis; ITSPhylogeneticRibosomal DNAPhytophthora parasitica is been considered one of the most prevalent citrus soilborn pathogens in Brazil. Fourteen isolates were collected in different region of São Paulo state. Amplification of the ITS region using primers ITS1 and ITS2 produced DNA fragments of 812 to 860 bp, that were compared with other sequences obtained to Gene Bank (NCBI). Neighbor-joining analysis, with 1000 bootstrap revealed an average similarity of 99,3 to 100% between the isolates, and 98,1 to 99,9% between S. Paulo state isolates and P. nicotianae (= Phytophthora parasitica) obtained from the Gene Bank (gi|8927482). The ITS1 was more conserved than to the ITS2. This is a good tool for quick diagnosis of Phytophthora citrus dise-ases.ESALQ/USP-Depto. de Entomologia Fitopatologia e Zoologia Agrícola Setor de Fitopatologia, Av. Pádua Dias, 11Departamento de Produção Vegetal FCA Universidade Estadual PaulistaCentro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira CAPTACSMDepartamento de Produção Vegetal FCA Universidade Estadual PaulistaUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)CAPTACSMRosa, Daniel DiasMachado, Marcos AntonioTargon, Maria Luisa Penteado NatividadeFurtado, Edson Luiz [UNESP]2022-04-29T07:51:57Z2022-04-29T07:51:57Z2006-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article188-191Summa Phytopathologica, v. 32, n. 2, p. 188-191, 2006.0100-5405http://hdl.handle.net/11449/2282042-s2.0-84987812275Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporSumma Phytopathologicainfo:eu-repo/semantics/openAccess2024-04-30T15:56:41Zoai:repositorio.unesp.br:11449/228204Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:00:49.076670Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Genotypic diversity among p. Parasitica isolates reveled by its-5.8s rdna nucleotide sequences.
Diversidade de phytophthora parasitica isolados de citrus usando seqüências de nucleotídeos da região its-5.8s rdna
title Genotypic diversity among p. Parasitica isolates reveled by its-5.8s rdna nucleotide sequences.
spellingShingle Genotypic diversity among p. Parasitica isolates reveled by its-5.8s rdna nucleotide sequences.
Rosa, Daniel Dias
Citrus gummosis; ITS
Phylogenetic
Ribosomal DNA
title_short Genotypic diversity among p. Parasitica isolates reveled by its-5.8s rdna nucleotide sequences.
title_full Genotypic diversity among p. Parasitica isolates reveled by its-5.8s rdna nucleotide sequences.
title_fullStr Genotypic diversity among p. Parasitica isolates reveled by its-5.8s rdna nucleotide sequences.
title_full_unstemmed Genotypic diversity among p. Parasitica isolates reveled by its-5.8s rdna nucleotide sequences.
title_sort Genotypic diversity among p. Parasitica isolates reveled by its-5.8s rdna nucleotide sequences.
author Rosa, Daniel Dias
author_facet Rosa, Daniel Dias
Machado, Marcos Antonio
Targon, Maria Luisa Penteado Natividade
Furtado, Edson Luiz [UNESP]
author_role author
author2 Machado, Marcos Antonio
Targon, Maria Luisa Penteado Natividade
Furtado, Edson Luiz [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (UNESP)
CAPTACSM
dc.contributor.author.fl_str_mv Rosa, Daniel Dias
Machado, Marcos Antonio
Targon, Maria Luisa Penteado Natividade
Furtado, Edson Luiz [UNESP]
dc.subject.por.fl_str_mv Citrus gummosis; ITS
Phylogenetic
Ribosomal DNA
topic Citrus gummosis; ITS
Phylogenetic
Ribosomal DNA
description Phytophthora parasitica is been considered one of the most prevalent citrus soilborn pathogens in Brazil. Fourteen isolates were collected in different region of São Paulo state. Amplification of the ITS region using primers ITS1 and ITS2 produced DNA fragments of 812 to 860 bp, that were compared with other sequences obtained to Gene Bank (NCBI). Neighbor-joining analysis, with 1000 bootstrap revealed an average similarity of 99,3 to 100% between the isolates, and 98,1 to 99,9% between S. Paulo state isolates and P. nicotianae (= Phytophthora parasitica) obtained from the Gene Bank (gi|8927482). The ITS1 was more conserved than to the ITS2. This is a good tool for quick diagnosis of Phytophthora citrus dise-ases.
publishDate 2006
dc.date.none.fl_str_mv 2006-01-01
2022-04-29T07:51:57Z
2022-04-29T07:51:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Summa Phytopathologica, v. 32, n. 2, p. 188-191, 2006.
0100-5405
http://hdl.handle.net/11449/228204
2-s2.0-84987812275
identifier_str_mv Summa Phytopathologica, v. 32, n. 2, p. 188-191, 2006.
0100-5405
2-s2.0-84987812275
url http://hdl.handle.net/11449/228204
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Summa Phytopathologica
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 188-191
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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