Physicochemical properties and effect of bioceramic root canal filling for primary teeth on osteoblast biology
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-7757-2020-0870 http://hdl.handle.net/11449/207708 |
Resumo: | OBJECTIVE: Bio-C Pulpecto (Bio-CP) was recently developed as the first bioceramic root filling material for primary teeth. To evaluate the physicochemical properties of radiopacity, setting time, pH, cytocompatibility and potential of Bio-CP to induce mineralisation, compared with (1) Calen thickened with zinc oxide (Calen-ZO), and (2) zinc oxide and eugenol (ZOE). METHODOLOGY: Physicochemical properties were evaluated according to ISO 6876. Saos-2 (human osteoblast-like cell line) exposed to extracts of the materials were subjected to assays of methyl thiazolyl tetrazolium, neutral red, alkaline phosphatase (ALP) activity and mineralised nodule production. The results were analysed using one-way or two-way ANOVA and Tukey's or Bonferroni's post-tests (α=0.05). RESULTS: All the materials showed radiopacity higher than 3 mm Al. Bio-CP had lower pH than Calen-ZO, but higher pH than ZOE. Calen-ZO and Bio-CP did not set. The setting time for ZOE was 110 min. The cytocompatibility order was Calen-ZO > Bio-CP > ZOE (1:2, 1:4 dilutions) and Calen-ZO > Bio-CP = ZOE (1:12, 1:24 dilutions) and Calen-ZO = Bio-CP > ZOE (1:32 dilution). Bio-CP induced greater ALP activity at 7 days, and greater mineralised nodule production, compared to Calen-ZO (p<0.05). Conclusions Bio-CP showed adequate physicochemical properties, cytocompatibility and potential to induce mineralisation. |
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Repositório Institucional da UNESP |
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2946 |
spelling |
Physicochemical properties and effect of bioceramic root canal filling for primary teeth on osteoblast biologyOBJECTIVE: Bio-C Pulpecto (Bio-CP) was recently developed as the first bioceramic root filling material for primary teeth. To evaluate the physicochemical properties of radiopacity, setting time, pH, cytocompatibility and potential of Bio-CP to induce mineralisation, compared with (1) Calen thickened with zinc oxide (Calen-ZO), and (2) zinc oxide and eugenol (ZOE). METHODOLOGY: Physicochemical properties were evaluated according to ISO 6876. Saos-2 (human osteoblast-like cell line) exposed to extracts of the materials were subjected to assays of methyl thiazolyl tetrazolium, neutral red, alkaline phosphatase (ALP) activity and mineralised nodule production. The results were analysed using one-way or two-way ANOVA and Tukey's or Bonferroni's post-tests (α=0.05). RESULTS: All the materials showed radiopacity higher than 3 mm Al. Bio-CP had lower pH than Calen-ZO, but higher pH than ZOE. Calen-ZO and Bio-CP did not set. The setting time for ZOE was 110 min. The cytocompatibility order was Calen-ZO > Bio-CP > ZOE (1:2, 1:4 dilutions) and Calen-ZO > Bio-CP = ZOE (1:12, 1:24 dilutions) and Calen-ZO = Bio-CP > ZOE (1:32 dilution). Bio-CP induced greater ALP activity at 7 days, and greater mineralised nodule production, compared to Calen-ZO (p<0.05). Conclusions Bio-CP showed adequate physicochemical properties, cytocompatibility and potential to induce mineralisation.Universidade Estadual Paulista Julio de Mesquita Fillho (UNESP) - Faculdade de Odontologia de Araraquara - Departamento de Odontologia Restauradora - Araraquara - SP - BrasilUniversidade Estadual Paulista Julio de Mesquita Fillho (UNESP) - Faculdade de Odontologia de Araraquara - Departamento de Odontologia Restauradora - Araraquara - SP - BrasilUniversidade Estadual Paulista (Unesp)Ochoa Rodríguez, Victor Manuel [UNESP]Tanomaru-Filho, Mario [UNESP]Rodrigues, Elisandra Márcia [UNESP]Bugança, Eduarda de Oliveira [UNESP]Guerreiro-Tanomaru, Juliane Maria [UNESP]Faria, Gisele [UNESP]2021-06-25T10:59:39Z2021-06-25T10:59:39Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlee20200870application/pdfhttp://dx.doi.org/10.1590/1678-7757-2020-0870Journal of applied oral science : revista FOB, v. 29, p. e20200870-.1678-7765http://hdl.handle.net/11449/20770810.1590/1678-7757-2020-0870S1678-775720210001004222-s2.0-85105470997S1678-77572021000100422.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of applied oral science : revista FOBinfo:eu-repo/semantics/openAccess2024-01-01T06:19:50Zoai:repositorio.unesp.br:11449/207708Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:51:13.885020Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Physicochemical properties and effect of bioceramic root canal filling for primary teeth on osteoblast biology |
title |
Physicochemical properties and effect of bioceramic root canal filling for primary teeth on osteoblast biology |
spellingShingle |
Physicochemical properties and effect of bioceramic root canal filling for primary teeth on osteoblast biology Ochoa Rodríguez, Victor Manuel [UNESP] |
title_short |
Physicochemical properties and effect of bioceramic root canal filling for primary teeth on osteoblast biology |
title_full |
Physicochemical properties and effect of bioceramic root canal filling for primary teeth on osteoblast biology |
title_fullStr |
Physicochemical properties and effect of bioceramic root canal filling for primary teeth on osteoblast biology |
title_full_unstemmed |
Physicochemical properties and effect of bioceramic root canal filling for primary teeth on osteoblast biology |
title_sort |
Physicochemical properties and effect of bioceramic root canal filling for primary teeth on osteoblast biology |
author |
Ochoa Rodríguez, Victor Manuel [UNESP] |
author_facet |
Ochoa Rodríguez, Victor Manuel [UNESP] Tanomaru-Filho, Mario [UNESP] Rodrigues, Elisandra Márcia [UNESP] Bugança, Eduarda de Oliveira [UNESP] Guerreiro-Tanomaru, Juliane Maria [UNESP] Faria, Gisele [UNESP] |
author_role |
author |
author2 |
Tanomaru-Filho, Mario [UNESP] Rodrigues, Elisandra Márcia [UNESP] Bugança, Eduarda de Oliveira [UNESP] Guerreiro-Tanomaru, Juliane Maria [UNESP] Faria, Gisele [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ochoa Rodríguez, Victor Manuel [UNESP] Tanomaru-Filho, Mario [UNESP] Rodrigues, Elisandra Márcia [UNESP] Bugança, Eduarda de Oliveira [UNESP] Guerreiro-Tanomaru, Juliane Maria [UNESP] Faria, Gisele [UNESP] |
description |
OBJECTIVE: Bio-C Pulpecto (Bio-CP) was recently developed as the first bioceramic root filling material for primary teeth. To evaluate the physicochemical properties of radiopacity, setting time, pH, cytocompatibility and potential of Bio-CP to induce mineralisation, compared with (1) Calen thickened with zinc oxide (Calen-ZO), and (2) zinc oxide and eugenol (ZOE). METHODOLOGY: Physicochemical properties were evaluated according to ISO 6876. Saos-2 (human osteoblast-like cell line) exposed to extracts of the materials were subjected to assays of methyl thiazolyl tetrazolium, neutral red, alkaline phosphatase (ALP) activity and mineralised nodule production. The results were analysed using one-way or two-way ANOVA and Tukey's or Bonferroni's post-tests (α=0.05). RESULTS: All the materials showed radiopacity higher than 3 mm Al. Bio-CP had lower pH than Calen-ZO, but higher pH than ZOE. Calen-ZO and Bio-CP did not set. The setting time for ZOE was 110 min. The cytocompatibility order was Calen-ZO > Bio-CP > ZOE (1:2, 1:4 dilutions) and Calen-ZO > Bio-CP = ZOE (1:12, 1:24 dilutions) and Calen-ZO = Bio-CP > ZOE (1:32 dilution). Bio-CP induced greater ALP activity at 7 days, and greater mineralised nodule production, compared to Calen-ZO (p<0.05). Conclusions Bio-CP showed adequate physicochemical properties, cytocompatibility and potential to induce mineralisation. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:59:39Z 2021-06-25T10:59:39Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-7757-2020-0870 Journal of applied oral science : revista FOB, v. 29, p. e20200870-. 1678-7765 http://hdl.handle.net/11449/207708 10.1590/1678-7757-2020-0870 S1678-77572021000100422 2-s2.0-85105470997 S1678-77572021000100422.pdf |
url |
http://dx.doi.org/10.1590/1678-7757-2020-0870 http://hdl.handle.net/11449/207708 |
identifier_str_mv |
Journal of applied oral science : revista FOB, v. 29, p. e20200870-. 1678-7765 10.1590/1678-7757-2020-0870 S1678-77572021000100422 2-s2.0-85105470997 S1678-77572021000100422.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of applied oral science : revista FOB |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
e20200870 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129366583934976 |