Physicochemical properties and effect of bioceramic root canal filling for primary teeth on osteoblast biology
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/188165 |
Resumo: | Bio-C Pulpecto (Bio-CP) was recently developed as the first bioceramic root filling material for primary teeth. Objective: To evaluate the physicochemical properties of radiopacity, setting time, pH, cytocompatibility and potential of Bio-CP to induce mineralisation, compared with (1) Calen thickened with zinc oxide (Calen-ZO), and (2) zinc oxide and eugenol (ZOE). Methodology: Physicochemical properties were evaluated according to ISO 6876. Saos-2 (human osteoblast-like cell line) exposed to extracts of the materials were subjected to assays of methyl thiazolyl tetrazolium, neutral red, alkaline phosphatase (ALP) activity and mineralised nodule production. The results were analysed using one-way or two-way ANOVA and Tukey’s or Bonferroni’s post-tests (α=0.05). Results: All the materials showed radiopacity higher than 3 mm Al. Bio-CP had lower pH than Calen-ZO, but higher pH than ZOE. Calen-ZO and Bio-CP did not set. The setting time for ZOE was 110 min. The cytocompatibility order was Calen-ZO > Bio-CP > ZOE (1:2, 1:4 dilutions) and Calen-ZO > Bio-CP = ZOE (1:12, 1:24 dilutions) and Calen-ZO = Bio-CP > ZOE (1:32 dilution). Bio-CP induced greater ALP activity at 7 days, and greater mineralised nodule production, compared to Calen-ZO (p<0.05). Conclusions Bio-CP showed adequate physicochemical properties, cytocompatibility and potential to induce mineralisation. |
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Journal of applied oral science (Online) |
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Physicochemical properties and effect of bioceramic root canal filling for primary teeth on osteoblast biologyCytotoxicityEndodonticsPrimary teethRoot Canal Filling MaterialsBio-C Pulpecto (Bio-CP) was recently developed as the first bioceramic root filling material for primary teeth. Objective: To evaluate the physicochemical properties of radiopacity, setting time, pH, cytocompatibility and potential of Bio-CP to induce mineralisation, compared with (1) Calen thickened with zinc oxide (Calen-ZO), and (2) zinc oxide and eugenol (ZOE). Methodology: Physicochemical properties were evaluated according to ISO 6876. Saos-2 (human osteoblast-like cell line) exposed to extracts of the materials were subjected to assays of methyl thiazolyl tetrazolium, neutral red, alkaline phosphatase (ALP) activity and mineralised nodule production. The results were analysed using one-way or two-way ANOVA and Tukey’s or Bonferroni’s post-tests (α=0.05). Results: All the materials showed radiopacity higher than 3 mm Al. Bio-CP had lower pH than Calen-ZO, but higher pH than ZOE. Calen-ZO and Bio-CP did not set. The setting time for ZOE was 110 min. The cytocompatibility order was Calen-ZO > Bio-CP > ZOE (1:2, 1:4 dilutions) and Calen-ZO > Bio-CP = ZOE (1:12, 1:24 dilutions) and Calen-ZO = Bio-CP > ZOE (1:32 dilution). Bio-CP induced greater ALP activity at 7 days, and greater mineralised nodule production, compared to Calen-ZO (p<0.05). Conclusions Bio-CP showed adequate physicochemical properties, cytocompatibility and potential to induce mineralisation.Universidade de São Paulo. Faculdade de Odontologia de Bauru2021-07-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/18816510.1590/1678-7757-2020-0870Journal of Applied Oral Science; Vol. 29 (2021); e20200870Journal of Applied Oral Science; Vol. 29 (2021); e20200870Journal of Applied Oral Science; v. 29 (2021); e202008701678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/188165/173796Copyright (c) 2021 Journal of Applied Oral Sciencehttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessOchoa Rodríguez, Victor Manuel Tanomaru-Filho, Mario Rodrigues, Elisandra Márcia Bagunça, Eduarda de OliveiraGuerreiro-Tanomaru, Juliane MariaFaria, Gisele 2021-07-05T23:05:00Zoai:revistas.usp.br:article/188165Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2021-07-05T23:05Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Physicochemical properties and effect of bioceramic root canal filling for primary teeth on osteoblast biology |
title |
Physicochemical properties and effect of bioceramic root canal filling for primary teeth on osteoblast biology |
spellingShingle |
Physicochemical properties and effect of bioceramic root canal filling for primary teeth on osteoblast biology Ochoa Rodríguez, Victor Manuel Cytotoxicity Endodontics Primary teeth Root Canal Filling Materials |
title_short |
Physicochemical properties and effect of bioceramic root canal filling for primary teeth on osteoblast biology |
title_full |
Physicochemical properties and effect of bioceramic root canal filling for primary teeth on osteoblast biology |
title_fullStr |
Physicochemical properties and effect of bioceramic root canal filling for primary teeth on osteoblast biology |
title_full_unstemmed |
Physicochemical properties and effect of bioceramic root canal filling for primary teeth on osteoblast biology |
title_sort |
Physicochemical properties and effect of bioceramic root canal filling for primary teeth on osteoblast biology |
author |
Ochoa Rodríguez, Victor Manuel |
author_facet |
Ochoa Rodríguez, Victor Manuel Tanomaru-Filho, Mario Rodrigues, Elisandra Márcia Bagunça, Eduarda de Oliveira Guerreiro-Tanomaru, Juliane Maria Faria, Gisele |
author_role |
author |
author2 |
Tanomaru-Filho, Mario Rodrigues, Elisandra Márcia Bagunça, Eduarda de Oliveira Guerreiro-Tanomaru, Juliane Maria Faria, Gisele |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Ochoa Rodríguez, Victor Manuel Tanomaru-Filho, Mario Rodrigues, Elisandra Márcia Bagunça, Eduarda de Oliveira Guerreiro-Tanomaru, Juliane Maria Faria, Gisele |
dc.subject.por.fl_str_mv |
Cytotoxicity Endodontics Primary teeth Root Canal Filling Materials |
topic |
Cytotoxicity Endodontics Primary teeth Root Canal Filling Materials |
description |
Bio-C Pulpecto (Bio-CP) was recently developed as the first bioceramic root filling material for primary teeth. Objective: To evaluate the physicochemical properties of radiopacity, setting time, pH, cytocompatibility and potential of Bio-CP to induce mineralisation, compared with (1) Calen thickened with zinc oxide (Calen-ZO), and (2) zinc oxide and eugenol (ZOE). Methodology: Physicochemical properties were evaluated according to ISO 6876. Saos-2 (human osteoblast-like cell line) exposed to extracts of the materials were subjected to assays of methyl thiazolyl tetrazolium, neutral red, alkaline phosphatase (ALP) activity and mineralised nodule production. The results were analysed using one-way or two-way ANOVA and Tukey’s or Bonferroni’s post-tests (α=0.05). Results: All the materials showed radiopacity higher than 3 mm Al. Bio-CP had lower pH than Calen-ZO, but higher pH than ZOE. Calen-ZO and Bio-CP did not set. The setting time for ZOE was 110 min. The cytocompatibility order was Calen-ZO > Bio-CP > ZOE (1:2, 1:4 dilutions) and Calen-ZO > Bio-CP = ZOE (1:12, 1:24 dilutions) and Calen-ZO = Bio-CP > ZOE (1:32 dilution). Bio-CP induced greater ALP activity at 7 days, and greater mineralised nodule production, compared to Calen-ZO (p<0.05). Conclusions Bio-CP showed adequate physicochemical properties, cytocompatibility and potential to induce mineralisation. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07-05 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/188165 10.1590/1678-7757-2020-0870 |
url |
https://www.revistas.usp.br/jaos/article/view/188165 |
identifier_str_mv |
10.1590/1678-7757-2020-0870 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/188165/173796 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Journal of Applied Oral Science http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 29 (2021); e20200870 Journal of Applied Oral Science; Vol. 29 (2021); e20200870 Journal of Applied Oral Science; v. 29 (2021); e20200870 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221681877254144 |