HER2/p53 ratios among canine mammary tumours and respective derived cell culture
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.29327/multiscience.2022001 http://hdl.handle.net/11449/246874 |
Resumo: | In this study, the expression of p53 and human epidermal growth factor receptor 2 (HER2) proteins were investigated in different canine mammary tumors (CMT) and cell cultures derived from them, for the purpose of determining neoplastic behavior in vitro. For this purpose, immunohistochemistry and immunocytochemistry were performed and results were compared according to histological diagnose. CMT were collected and divided into two halves, one for histological and immunohistochemistry analyses and another for cell cultures. Cell markers p53 and HER2 proteins were detected by immunohistochemistry in the original tumors and immunocytochemistry in CMT-derived cell lines. A total of CMT (n = 17) were classified and divided into benign mixed tumor (BMT), complex carcinoma (CC), simple carcinoma (SC), and squamous cell carcinoma (SCC). Low expression of p53 was verified in BMT and CC, averagely in SC and undetectable in SCC. In vitro, there was an increase in p53 expression by cultured cells. However, HER2 protein expression was not significant in BMT, CC and SCC. In contrast, MCT-derived cells were considered HER2-positivein all MCT types. In conclusion, the in vitro model is essential for the study of neoplastic behavior and understanding of the carcinogenic mechanisms that occur in vivo. |
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HER2/p53 ratios among canine mammary tumours and respective derived cell culturecancer markerscell phenotypeIn vitro cultureIn this study, the expression of p53 and human epidermal growth factor receptor 2 (HER2) proteins were investigated in different canine mammary tumors (CMT) and cell cultures derived from them, for the purpose of determining neoplastic behavior in vitro. For this purpose, immunohistochemistry and immunocytochemistry were performed and results were compared according to histological diagnose. CMT were collected and divided into two halves, one for histological and immunohistochemistry analyses and another for cell cultures. Cell markers p53 and HER2 proteins were detected by immunohistochemistry in the original tumors and immunocytochemistry in CMT-derived cell lines. A total of CMT (n = 17) were classified and divided into benign mixed tumor (BMT), complex carcinoma (CC), simple carcinoma (SC), and squamous cell carcinoma (SCC). Low expression of p53 was verified in BMT and CC, averagely in SC and undetectable in SCC. In vitro, there was an increase in p53 expression by cultured cells. However, HER2 protein expression was not significant in BMT, CC and SCC. In contrast, MCT-derived cells were considered HER2-positivein all MCT types. In conclusion, the in vitro model is essential for the study of neoplastic behavior and understanding of the carcinogenic mechanisms that occur in vivo.Catholic University of Tocantins (FACTO)Sao Paulo State University Júlio de Mesquita Filho (UNESP)Sao Paulo State University Júlio de Mesquita Filho (UNESP)Catholic University of Tocantins (FACTO)Universidade Estadual Paulista (UNESP)Sakamoto, Silmara SanaePanegossi, Letícia Colin [UNESP]De Carvalho, Roberto Gameiro [UNESP]Cardoso, Tereza Cristina [UNESP]2023-07-29T12:53:00Z2023-07-29T12:53:00Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.29327/multiscience.2022001Multidisciplinary Science Journal, v. 4, n. 1, 2022.2675-1240http://hdl.handle.net/11449/24687410.29327/multiscience.20220012-s2.0-85148618203Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMultidisciplinary Science Journalinfo:eu-repo/semantics/openAccess2023-07-29T12:53:00Zoai:repositorio.unesp.br:11449/246874Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:27:34.733788Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
HER2/p53 ratios among canine mammary tumours and respective derived cell culture |
title |
HER2/p53 ratios among canine mammary tumours and respective derived cell culture |
spellingShingle |
HER2/p53 ratios among canine mammary tumours and respective derived cell culture Sakamoto, Silmara Sanae cancer markers cell phenotype In vitro culture |
title_short |
HER2/p53 ratios among canine mammary tumours and respective derived cell culture |
title_full |
HER2/p53 ratios among canine mammary tumours and respective derived cell culture |
title_fullStr |
HER2/p53 ratios among canine mammary tumours and respective derived cell culture |
title_full_unstemmed |
HER2/p53 ratios among canine mammary tumours and respective derived cell culture |
title_sort |
HER2/p53 ratios among canine mammary tumours and respective derived cell culture |
author |
Sakamoto, Silmara Sanae |
author_facet |
Sakamoto, Silmara Sanae Panegossi, Letícia Colin [UNESP] De Carvalho, Roberto Gameiro [UNESP] Cardoso, Tereza Cristina [UNESP] |
author_role |
author |
author2 |
Panegossi, Letícia Colin [UNESP] De Carvalho, Roberto Gameiro [UNESP] Cardoso, Tereza Cristina [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Catholic University of Tocantins (FACTO) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Sakamoto, Silmara Sanae Panegossi, Letícia Colin [UNESP] De Carvalho, Roberto Gameiro [UNESP] Cardoso, Tereza Cristina [UNESP] |
dc.subject.por.fl_str_mv |
cancer markers cell phenotype In vitro culture |
topic |
cancer markers cell phenotype In vitro culture |
description |
In this study, the expression of p53 and human epidermal growth factor receptor 2 (HER2) proteins were investigated in different canine mammary tumors (CMT) and cell cultures derived from them, for the purpose of determining neoplastic behavior in vitro. For this purpose, immunohistochemistry and immunocytochemistry were performed and results were compared according to histological diagnose. CMT were collected and divided into two halves, one for histological and immunohistochemistry analyses and another for cell cultures. Cell markers p53 and HER2 proteins were detected by immunohistochemistry in the original tumors and immunocytochemistry in CMT-derived cell lines. A total of CMT (n = 17) were classified and divided into benign mixed tumor (BMT), complex carcinoma (CC), simple carcinoma (SC), and squamous cell carcinoma (SCC). Low expression of p53 was verified in BMT and CC, averagely in SC and undetectable in SCC. In vitro, there was an increase in p53 expression by cultured cells. However, HER2 protein expression was not significant in BMT, CC and SCC. In contrast, MCT-derived cells were considered HER2-positivein all MCT types. In conclusion, the in vitro model is essential for the study of neoplastic behavior and understanding of the carcinogenic mechanisms that occur in vivo. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 2023-07-29T12:53:00Z 2023-07-29T12:53:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.29327/multiscience.2022001 Multidisciplinary Science Journal, v. 4, n. 1, 2022. 2675-1240 http://hdl.handle.net/11449/246874 10.29327/multiscience.2022001 2-s2.0-85148618203 |
url |
http://dx.doi.org/10.29327/multiscience.2022001 http://hdl.handle.net/11449/246874 |
identifier_str_mv |
Multidisciplinary Science Journal, v. 4, n. 1, 2022. 2675-1240 10.29327/multiscience.2022001 2-s2.0-85148618203 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Multidisciplinary Science Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129204694286336 |