HER2/p53 ratios among canine mammary tumours and respective derived cell culture

Detalhes bibliográficos
Autor(a) principal: Sakamoto, Silmara Sanae
Data de Publicação: 2022
Outros Autores: Panegossi, Letícia Colin [UNESP], De Carvalho, Roberto Gameiro [UNESP], Cardoso, Tereza Cristina [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.29327/multiscience.2022001
http://hdl.handle.net/11449/246874
Resumo: In this study, the expression of p53 and human epidermal growth factor receptor 2 (HER2) proteins were investigated in different canine mammary tumors (CMT) and cell cultures derived from them, for the purpose of determining neoplastic behavior in vitro. For this purpose, immunohistochemistry and immunocytochemistry were performed and results were compared according to histological diagnose. CMT were collected and divided into two halves, one for histological and immunohistochemistry analyses and another for cell cultures. Cell markers p53 and HER2 proteins were detected by immunohistochemistry in the original tumors and immunocytochemistry in CMT-derived cell lines. A total of CMT (n = 17) were classified and divided into benign mixed tumor (BMT), complex carcinoma (CC), simple carcinoma (SC), and squamous cell carcinoma (SCC). Low expression of p53 was verified in BMT and CC, averagely in SC and undetectable in SCC. In vitro, there was an increase in p53 expression by cultured cells. However, HER2 protein expression was not significant in BMT, CC and SCC. In contrast, MCT-derived cells were considered HER2-positivein all MCT types. In conclusion, the in vitro model is essential for the study of neoplastic behavior and understanding of the carcinogenic mechanisms that occur in vivo.
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spelling HER2/p53 ratios among canine mammary tumours and respective derived cell culturecancer markerscell phenotypeIn vitro cultureIn this study, the expression of p53 and human epidermal growth factor receptor 2 (HER2) proteins were investigated in different canine mammary tumors (CMT) and cell cultures derived from them, for the purpose of determining neoplastic behavior in vitro. For this purpose, immunohistochemistry and immunocytochemistry were performed and results were compared according to histological diagnose. CMT were collected and divided into two halves, one for histological and immunohistochemistry analyses and another for cell cultures. Cell markers p53 and HER2 proteins were detected by immunohistochemistry in the original tumors and immunocytochemistry in CMT-derived cell lines. A total of CMT (n = 17) were classified and divided into benign mixed tumor (BMT), complex carcinoma (CC), simple carcinoma (SC), and squamous cell carcinoma (SCC). Low expression of p53 was verified in BMT and CC, averagely in SC and undetectable in SCC. In vitro, there was an increase in p53 expression by cultured cells. However, HER2 protein expression was not significant in BMT, CC and SCC. In contrast, MCT-derived cells were considered HER2-positivein all MCT types. In conclusion, the in vitro model is essential for the study of neoplastic behavior and understanding of the carcinogenic mechanisms that occur in vivo.Catholic University of Tocantins (FACTO)Sao Paulo State University Júlio de Mesquita Filho (UNESP)Sao Paulo State University Júlio de Mesquita Filho (UNESP)Catholic University of Tocantins (FACTO)Universidade Estadual Paulista (UNESP)Sakamoto, Silmara SanaePanegossi, Letícia Colin [UNESP]De Carvalho, Roberto Gameiro [UNESP]Cardoso, Tereza Cristina [UNESP]2023-07-29T12:53:00Z2023-07-29T12:53:00Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.29327/multiscience.2022001Multidisciplinary Science Journal, v. 4, n. 1, 2022.2675-1240http://hdl.handle.net/11449/24687410.29327/multiscience.20220012-s2.0-85148618203Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMultidisciplinary Science Journalinfo:eu-repo/semantics/openAccess2023-07-29T12:53:00Zoai:repositorio.unesp.br:11449/246874Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:27:34.733788Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv HER2/p53 ratios among canine mammary tumours and respective derived cell culture
title HER2/p53 ratios among canine mammary tumours and respective derived cell culture
spellingShingle HER2/p53 ratios among canine mammary tumours and respective derived cell culture
Sakamoto, Silmara Sanae
cancer markers
cell phenotype
In vitro culture
title_short HER2/p53 ratios among canine mammary tumours and respective derived cell culture
title_full HER2/p53 ratios among canine mammary tumours and respective derived cell culture
title_fullStr HER2/p53 ratios among canine mammary tumours and respective derived cell culture
title_full_unstemmed HER2/p53 ratios among canine mammary tumours and respective derived cell culture
title_sort HER2/p53 ratios among canine mammary tumours and respective derived cell culture
author Sakamoto, Silmara Sanae
author_facet Sakamoto, Silmara Sanae
Panegossi, Letícia Colin [UNESP]
De Carvalho, Roberto Gameiro [UNESP]
Cardoso, Tereza Cristina [UNESP]
author_role author
author2 Panegossi, Letícia Colin [UNESP]
De Carvalho, Roberto Gameiro [UNESP]
Cardoso, Tereza Cristina [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Catholic University of Tocantins (FACTO)
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Sakamoto, Silmara Sanae
Panegossi, Letícia Colin [UNESP]
De Carvalho, Roberto Gameiro [UNESP]
Cardoso, Tereza Cristina [UNESP]
dc.subject.por.fl_str_mv cancer markers
cell phenotype
In vitro culture
topic cancer markers
cell phenotype
In vitro culture
description In this study, the expression of p53 and human epidermal growth factor receptor 2 (HER2) proteins were investigated in different canine mammary tumors (CMT) and cell cultures derived from them, for the purpose of determining neoplastic behavior in vitro. For this purpose, immunohistochemistry and immunocytochemistry were performed and results were compared according to histological diagnose. CMT were collected and divided into two halves, one for histological and immunohistochemistry analyses and another for cell cultures. Cell markers p53 and HER2 proteins were detected by immunohistochemistry in the original tumors and immunocytochemistry in CMT-derived cell lines. A total of CMT (n = 17) were classified and divided into benign mixed tumor (BMT), complex carcinoma (CC), simple carcinoma (SC), and squamous cell carcinoma (SCC). Low expression of p53 was verified in BMT and CC, averagely in SC and undetectable in SCC. In vitro, there was an increase in p53 expression by cultured cells. However, HER2 protein expression was not significant in BMT, CC and SCC. In contrast, MCT-derived cells were considered HER2-positivein all MCT types. In conclusion, the in vitro model is essential for the study of neoplastic behavior and understanding of the carcinogenic mechanisms that occur in vivo.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
2023-07-29T12:53:00Z
2023-07-29T12:53:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.29327/multiscience.2022001
Multidisciplinary Science Journal, v. 4, n. 1, 2022.
2675-1240
http://hdl.handle.net/11449/246874
10.29327/multiscience.2022001
2-s2.0-85148618203
url http://dx.doi.org/10.29327/multiscience.2022001
http://hdl.handle.net/11449/246874
identifier_str_mv Multidisciplinary Science Journal, v. 4, n. 1, 2022.
2675-1240
10.29327/multiscience.2022001
2-s2.0-85148618203
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Multidisciplinary Science Journal
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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