Melatonin decreases estrogen receptor binding to estrogen response elements sites on the oct4 gene in human breast cancer stem cells
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.18632/genesandcancer.107 http://hdl.handle.net/11449/231390 |
Resumo: | Cancer stem cells (CSCs) pose a challenge in cancer treatment, as these cells can drive tumor growth and are resistant to chemotherapy. Melatonin exerts its oncostatic effects through the estrogen receptor (ER) pathway in cancer cells, however its action in CSCs is unclear. Here, we evaluated the effect of melatonin on the regulation of the transcription factor OCT4 (Octamer Binding 4) by estrogen receptor alpha (ERα) in breast cancer stem cells (BCSCs). The cells were grown as a cell suspension or as anchorage independent growth, for the mammospheres growth, representing the CSCs population and treated with 10 nM estrogen (E2) or 10 µM of the environmental estrogen Bisphenol A (BPA) and 1 mM of melatonin. At the end, the cell growth as well as OCT4 and ERα expression and the binding activity of ERα to the OCT4 was assessed. The increase in number and size of mammospheres induced by E2 or BPA was reduced by melatonin treatment. Furthermore, binding of the ERα to OCT4 was reduced, accompanied by a reduction of OCT4 and ERα expression. Thus, melatonin treatment is effective against proliferation of BCSCs in vitro and impacts the ER pathway, demonstrating its potential therapeutic use in breast cancer. |
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Melatonin decreases estrogen receptor binding to estrogen response elements sites on the oct4 gene in human breast cancer stem cellsChromatin immunoprecipitationEstrogen receptorMammospheresMelatoninThree-dimensional growthCancer stem cells (CSCs) pose a challenge in cancer treatment, as these cells can drive tumor growth and are resistant to chemotherapy. Melatonin exerts its oncostatic effects through the estrogen receptor (ER) pathway in cancer cells, however its action in CSCs is unclear. Here, we evaluated the effect of melatonin on the regulation of the transcription factor OCT4 (Octamer Binding 4) by estrogen receptor alpha (ERα) in breast cancer stem cells (BCSCs). The cells were grown as a cell suspension or as anchorage independent growth, for the mammospheres growth, representing the CSCs population and treated with 10 nM estrogen (E2) or 10 µM of the environmental estrogen Bisphenol A (BPA) and 1 mM of melatonin. At the end, the cell growth as well as OCT4 and ERα expression and the binding activity of ERα to the OCT4 was assessed. The increase in number and size of mammospheres induced by E2 or BPA was reduced by melatonin treatment. Furthermore, binding of the ERα to OCT4 was reduced, accompanied by a reduction of OCT4 and ERα expression. Thus, melatonin treatment is effective against proliferation of BCSCs in vitro and impacts the ER pathway, demonstrating its potential therapeutic use in breast cancer.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Biology Universidade Estadual Paulista “Júlio de Mesquita Filho”Department of Biochemistry and Molecular Biology Michigan State UniversityDepartment of Pediatrics and Human Development Michigan State UniversityDepartment of Molecular Biology Faculdade de Medicina de São José do Rio PretoDepartment of Biology Universidade Estadual Paulista “Júlio de Mesquita Filho”Universidade Estadual Paulista (UNESP)Michigan State UniversityFaculdade de Medicina de São José do Rio PretoLopes, Juliana [UNESP]Arnosti, DavidTrosko, James E.Tai, Mei-HuiZuccari, Debora [UNESP]2022-04-29T08:45:03Z2022-04-29T08:45:03Z2016-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article209-217http://dx.doi.org/10.18632/genesandcancer.107Genes and Cancer, v. 7, n. 5-6, p. 209-217, 2016.1947-60271947-6019http://hdl.handle.net/11449/23139010.18632/genesandcancer.1072-s2.0-85006646858Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenes and Cancerinfo:eu-repo/semantics/openAccess2022-04-29T08:45:03Zoai:repositorio.unesp.br:11449/231390Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:22:44.828526Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Melatonin decreases estrogen receptor binding to estrogen response elements sites on the oct4 gene in human breast cancer stem cells |
title |
Melatonin decreases estrogen receptor binding to estrogen response elements sites on the oct4 gene in human breast cancer stem cells |
spellingShingle |
Melatonin decreases estrogen receptor binding to estrogen response elements sites on the oct4 gene in human breast cancer stem cells Lopes, Juliana [UNESP] Chromatin immunoprecipitation Estrogen receptor Mammospheres Melatonin Three-dimensional growth |
title_short |
Melatonin decreases estrogen receptor binding to estrogen response elements sites on the oct4 gene in human breast cancer stem cells |
title_full |
Melatonin decreases estrogen receptor binding to estrogen response elements sites on the oct4 gene in human breast cancer stem cells |
title_fullStr |
Melatonin decreases estrogen receptor binding to estrogen response elements sites on the oct4 gene in human breast cancer stem cells |
title_full_unstemmed |
Melatonin decreases estrogen receptor binding to estrogen response elements sites on the oct4 gene in human breast cancer stem cells |
title_sort |
Melatonin decreases estrogen receptor binding to estrogen response elements sites on the oct4 gene in human breast cancer stem cells |
author |
Lopes, Juliana [UNESP] |
author_facet |
Lopes, Juliana [UNESP] Arnosti, David Trosko, James E. Tai, Mei-Hui Zuccari, Debora [UNESP] |
author_role |
author |
author2 |
Arnosti, David Trosko, James E. Tai, Mei-Hui Zuccari, Debora [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Michigan State University Faculdade de Medicina de São José do Rio Preto |
dc.contributor.author.fl_str_mv |
Lopes, Juliana [UNESP] Arnosti, David Trosko, James E. Tai, Mei-Hui Zuccari, Debora [UNESP] |
dc.subject.por.fl_str_mv |
Chromatin immunoprecipitation Estrogen receptor Mammospheres Melatonin Three-dimensional growth |
topic |
Chromatin immunoprecipitation Estrogen receptor Mammospheres Melatonin Three-dimensional growth |
description |
Cancer stem cells (CSCs) pose a challenge in cancer treatment, as these cells can drive tumor growth and are resistant to chemotherapy. Melatonin exerts its oncostatic effects through the estrogen receptor (ER) pathway in cancer cells, however its action in CSCs is unclear. Here, we evaluated the effect of melatonin on the regulation of the transcription factor OCT4 (Octamer Binding 4) by estrogen receptor alpha (ERα) in breast cancer stem cells (BCSCs). The cells were grown as a cell suspension or as anchorage independent growth, for the mammospheres growth, representing the CSCs population and treated with 10 nM estrogen (E2) or 10 µM of the environmental estrogen Bisphenol A (BPA) and 1 mM of melatonin. At the end, the cell growth as well as OCT4 and ERα expression and the binding activity of ERα to the OCT4 was assessed. The increase in number and size of mammospheres induced by E2 or BPA was reduced by melatonin treatment. Furthermore, binding of the ERα to OCT4 was reduced, accompanied by a reduction of OCT4 and ERα expression. Thus, melatonin treatment is effective against proliferation of BCSCs in vitro and impacts the ER pathway, demonstrating its potential therapeutic use in breast cancer. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-05-01 2022-04-29T08:45:03Z 2022-04-29T08:45:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.18632/genesandcancer.107 Genes and Cancer, v. 7, n. 5-6, p. 209-217, 2016. 1947-6027 1947-6019 http://hdl.handle.net/11449/231390 10.18632/genesandcancer.107 2-s2.0-85006646858 |
url |
http://dx.doi.org/10.18632/genesandcancer.107 http://hdl.handle.net/11449/231390 |
identifier_str_mv |
Genes and Cancer, v. 7, n. 5-6, p. 209-217, 2016. 1947-6027 1947-6019 10.18632/genesandcancer.107 2-s2.0-85006646858 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genes and Cancer |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
209-217 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128641470562304 |