Estudo da enxertia hipocotiledonar do maracujazeiro-amarelo sobre dois porta-enxertos, através de microscopia eletrônica de varredura
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/231956 |
Resumo: | The objective of this work was to verify how much time after the accomplishment of the hypocotyledonary grafting occur the weld or the adhesion between graft and rootstock, and also the formation of the callus bridge, verified for the total fulfilling of the slot for secondary meristematic tissue (callus). It was produced 56 seedlings of yellow passion fruit (P. edulis Sims f. flavicarpa Deg.) by the hypocotyledonary grafting in full crack in the top on two rootstocks (P. edulis Sims f. flavicarpa Deg. e P. alata Dryander). On the 0, 3, 6, 9, 12, 15 and 18 days after grafting, it was collected the region of the grafting of four seedlings of each combination. The collected material was fixed in a solution of glutaraldehyde at 3%, post-fixed in osmium tetroxide at 2%, dehydrated in a series of ethyl alcohol (30, 40, 50, 60, 70, 80, 90, 100%), led to the drier of critical point (CO2), set up, metalized with gold - palladium (35 nm) and, finally, observed and eletromicrographed in a scanning electronic microscope JEOL JSM 5410 (operated at 15 kV). It was discovered that, six days after grafting, the welding for the rootstock P. alata had already occurred, which was only evidenced by P. edulis f. flavicarpa on the ninth days. Also, on the ninth day, it was observed on both rootstocks, a complete callus bridge formation, indicating that, from this point, you can start the acclimation process of the seedling, to take it to an environment of lower humidity. |
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Estudo da enxertia hipocotiledonar do maracujazeiro-amarelo sobre dois porta-enxertos, através de microscopia eletrônica de varreduraStudy of hypocotyledonary grafting of the passion fruit plant on two rootstocks, through scanning electronic microscopyCompatibilityElectromicrographicPassiflora spp.Sweet passion fruit plantVegetative propagationThe objective of this work was to verify how much time after the accomplishment of the hypocotyledonary grafting occur the weld or the adhesion between graft and rootstock, and also the formation of the callus bridge, verified for the total fulfilling of the slot for secondary meristematic tissue (callus). It was produced 56 seedlings of yellow passion fruit (P. edulis Sims f. flavicarpa Deg.) by the hypocotyledonary grafting in full crack in the top on two rootstocks (P. edulis Sims f. flavicarpa Deg. e P. alata Dryander). On the 0, 3, 6, 9, 12, 15 and 18 days after grafting, it was collected the region of the grafting of four seedlings of each combination. The collected material was fixed in a solution of glutaraldehyde at 3%, post-fixed in osmium tetroxide at 2%, dehydrated in a series of ethyl alcohol (30, 40, 50, 60, 70, 80, 90, 100%), led to the drier of critical point (CO2), set up, metalized with gold - palladium (35 nm) and, finally, observed and eletromicrographed in a scanning electronic microscope JEOL JSM 5410 (operated at 15 kV). It was discovered that, six days after grafting, the welding for the rootstock P. alata had already occurred, which was only evidenced by P. edulis f. flavicarpa on the ninth days. Also, on the ninth day, it was observed on both rootstocks, a complete callus bridge formation, indicating that, from this point, you can start the acclimation process of the seedling, to take it to an environment of lower humidity.Embrapa Acre, Rodovia BR 364 km 14, Zona Rural, Cep 69901180 Rio Branco-ACUnesp/Fcav Via de acesso Paulo Donato Castellane, Cep 14884-900 Jaboticabal-SPUnesp/Fcav Via de acesso Paulo Donato Castellane, Cep 14884-900 Jaboticabal-SPEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual Paulista (UNESP)Filho, Geraldo Costa NogueiraRoncatto, GivanildoRuggiero, Carlos [UNESP]de Oliveira, João Carlos [UNESP]Malheiros, Euclides Braga [UNESP]2022-04-29T08:48:20Z2022-04-29T08:48:20Z2010-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article40Revista Brasileira de Fruticultura, v. 32, n. 2, p. 40-, 2010.0100-2945http://hdl.handle.net/11449/2319562-s2.0-77957917248Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporRevista Brasileira de Fruticulturainfo:eu-repo/semantics/openAccess2024-06-07T13:56:41Zoai:repositorio.unesp.br:11449/231956Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:29:59.577186Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Estudo da enxertia hipocotiledonar do maracujazeiro-amarelo sobre dois porta-enxertos, através de microscopia eletrônica de varredura Study of hypocotyledonary grafting of the passion fruit plant on two rootstocks, through scanning electronic microscopy |
title |
Estudo da enxertia hipocotiledonar do maracujazeiro-amarelo sobre dois porta-enxertos, através de microscopia eletrônica de varredura |
spellingShingle |
Estudo da enxertia hipocotiledonar do maracujazeiro-amarelo sobre dois porta-enxertos, através de microscopia eletrônica de varredura Filho, Geraldo Costa Nogueira Compatibility Electromicrographic Passiflora spp. Sweet passion fruit plant Vegetative propagation |
title_short |
Estudo da enxertia hipocotiledonar do maracujazeiro-amarelo sobre dois porta-enxertos, através de microscopia eletrônica de varredura |
title_full |
Estudo da enxertia hipocotiledonar do maracujazeiro-amarelo sobre dois porta-enxertos, através de microscopia eletrônica de varredura |
title_fullStr |
Estudo da enxertia hipocotiledonar do maracujazeiro-amarelo sobre dois porta-enxertos, através de microscopia eletrônica de varredura |
title_full_unstemmed |
Estudo da enxertia hipocotiledonar do maracujazeiro-amarelo sobre dois porta-enxertos, através de microscopia eletrônica de varredura |
title_sort |
Estudo da enxertia hipocotiledonar do maracujazeiro-amarelo sobre dois porta-enxertos, através de microscopia eletrônica de varredura |
author |
Filho, Geraldo Costa Nogueira |
author_facet |
Filho, Geraldo Costa Nogueira Roncatto, Givanildo Ruggiero, Carlos [UNESP] de Oliveira, João Carlos [UNESP] Malheiros, Euclides Braga [UNESP] |
author_role |
author |
author2 |
Roncatto, Givanildo Ruggiero, Carlos [UNESP] de Oliveira, João Carlos [UNESP] Malheiros, Euclides Braga [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Filho, Geraldo Costa Nogueira Roncatto, Givanildo Ruggiero, Carlos [UNESP] de Oliveira, João Carlos [UNESP] Malheiros, Euclides Braga [UNESP] |
dc.subject.por.fl_str_mv |
Compatibility Electromicrographic Passiflora spp. Sweet passion fruit plant Vegetative propagation |
topic |
Compatibility Electromicrographic Passiflora spp. Sweet passion fruit plant Vegetative propagation |
description |
The objective of this work was to verify how much time after the accomplishment of the hypocotyledonary grafting occur the weld or the adhesion between graft and rootstock, and also the formation of the callus bridge, verified for the total fulfilling of the slot for secondary meristematic tissue (callus). It was produced 56 seedlings of yellow passion fruit (P. edulis Sims f. flavicarpa Deg.) by the hypocotyledonary grafting in full crack in the top on two rootstocks (P. edulis Sims f. flavicarpa Deg. e P. alata Dryander). On the 0, 3, 6, 9, 12, 15 and 18 days after grafting, it was collected the region of the grafting of four seedlings of each combination. The collected material was fixed in a solution of glutaraldehyde at 3%, post-fixed in osmium tetroxide at 2%, dehydrated in a series of ethyl alcohol (30, 40, 50, 60, 70, 80, 90, 100%), led to the drier of critical point (CO2), set up, metalized with gold - palladium (35 nm) and, finally, observed and eletromicrographed in a scanning electronic microscope JEOL JSM 5410 (operated at 15 kV). It was discovered that, six days after grafting, the welding for the rootstock P. alata had already occurred, which was only evidenced by P. edulis f. flavicarpa on the ninth days. Also, on the ninth day, it was observed on both rootstocks, a complete callus bridge formation, indicating that, from this point, you can start the acclimation process of the seedling, to take it to an environment of lower humidity. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-06-01 2022-04-29T08:48:20Z 2022-04-29T08:48:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Revista Brasileira de Fruticultura, v. 32, n. 2, p. 40-, 2010. 0100-2945 http://hdl.handle.net/11449/231956 2-s2.0-77957917248 |
identifier_str_mv |
Revista Brasileira de Fruticultura, v. 32, n. 2, p. 40-, 2010. 0100-2945 2-s2.0-77957917248 |
url |
http://hdl.handle.net/11449/231956 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Revista Brasileira de Fruticultura |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
40 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129210341916672 |