Fast Determination of Short-Chain Fatty Acids and Glucose Simultaneously by Ultraviolet/Visible and Refraction Index Detectors via High-Performance Liquid Chromatography

Detalhes bibliográficos
Autor(a) principal: Serafim, Jéssica Aparecida [UNESP]
Data de Publicação: 2021
Outros Autores: Silveira, Raiza Felismino [UNESP], Vicente, Eduardo Festozo [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s12161-021-01990-w
http://hdl.handle.net/11449/205918
Resumo: A rapid analytical method for simultaneous quantification of glucose (GLC) and seven short-chain fatty acids (SCFA) was developed for detection of ruminal fermentation parameters. The formic acid (FA), lactic acid (LA), acetic acid (AA), propionic acid (PA), butyric acid (BA), isovaleric acid (IVA), valeric acid (VA), and glucose were identified and quantified by high-performance liquid chromatography (HPLC) technique, using a reverse-phase column (C-18) and simultaneous detection in UV-Vis and Refraction Index (RID) detectors, linked in series. The method was validated following the requirements of selectivity, linearity, sensitivity, limit of detection, limit of quantification, accuracy, and precision. The developed method operates in isocratic and gradient modes, providing a better SCFA separation, which allows their determination with a high accuracy and repeatability. Therefore, this study provides a fast, reliable, accurate, straightforward, and efficient alternative method for analysis of ruminal fermentation parameters by liquid chromatography using both detectors simultaneously.
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spelling Fast Determination of Short-Chain Fatty Acids and Glucose Simultaneously by Ultraviolet/Visible and Refraction Index Detectors via High-Performance Liquid ChromatographyAnalysisHPLCRIDRuminal fermentationSCFAUV-VisA rapid analytical method for simultaneous quantification of glucose (GLC) and seven short-chain fatty acids (SCFA) was developed for detection of ruminal fermentation parameters. The formic acid (FA), lactic acid (LA), acetic acid (AA), propionic acid (PA), butyric acid (BA), isovaleric acid (IVA), valeric acid (VA), and glucose were identified and quantified by high-performance liquid chromatography (HPLC) technique, using a reverse-phase column (C-18) and simultaneous detection in UV-Vis and Refraction Index (RID) detectors, linked in series. The method was validated following the requirements of selectivity, linearity, sensitivity, limit of detection, limit of quantification, accuracy, and precision. The developed method operates in isocratic and gradient modes, providing a better SCFA separation, which allows their determination with a high accuracy and repeatability. Therefore, this study provides a fast, reliable, accurate, straightforward, and efficient alternative method for analysis of ruminal fermentation parameters by liquid chromatography using both detectors simultaneously.São Paulo State University (Unesp) School of Sciences and EngineeringSão Paulo State University (Unesp) School of Agricultural and Veterinarian SciencesSão Paulo State University (Unesp) School of Sciences and EngineeringSão Paulo State University (Unesp) School of Agricultural and Veterinarian SciencesUniversidade Estadual Paulista (Unesp)Serafim, Jéssica Aparecida [UNESP]Silveira, Raiza Felismino [UNESP]Vicente, Eduardo Festozo [UNESP]2021-06-25T10:23:32Z2021-06-25T10:23:32Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s12161-021-01990-wFood Analytical Methods.1936-976X1936-9751http://hdl.handle.net/11449/20591810.1007/s12161-021-01990-w2-s2.0-85101211661Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFood Analytical Methodsinfo:eu-repo/semantics/openAccess2021-10-22T19:58:00Zoai:repositorio.unesp.br:11449/205918Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:08:11.262357Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Fast Determination of Short-Chain Fatty Acids and Glucose Simultaneously by Ultraviolet/Visible and Refraction Index Detectors via High-Performance Liquid Chromatography
title Fast Determination of Short-Chain Fatty Acids and Glucose Simultaneously by Ultraviolet/Visible and Refraction Index Detectors via High-Performance Liquid Chromatography
spellingShingle Fast Determination of Short-Chain Fatty Acids and Glucose Simultaneously by Ultraviolet/Visible and Refraction Index Detectors via High-Performance Liquid Chromatography
Serafim, Jéssica Aparecida [UNESP]
Analysis
HPLC
RID
Ruminal fermentation
SCFA
UV-Vis
title_short Fast Determination of Short-Chain Fatty Acids and Glucose Simultaneously by Ultraviolet/Visible and Refraction Index Detectors via High-Performance Liquid Chromatography
title_full Fast Determination of Short-Chain Fatty Acids and Glucose Simultaneously by Ultraviolet/Visible and Refraction Index Detectors via High-Performance Liquid Chromatography
title_fullStr Fast Determination of Short-Chain Fatty Acids and Glucose Simultaneously by Ultraviolet/Visible and Refraction Index Detectors via High-Performance Liquid Chromatography
title_full_unstemmed Fast Determination of Short-Chain Fatty Acids and Glucose Simultaneously by Ultraviolet/Visible and Refraction Index Detectors via High-Performance Liquid Chromatography
title_sort Fast Determination of Short-Chain Fatty Acids and Glucose Simultaneously by Ultraviolet/Visible and Refraction Index Detectors via High-Performance Liquid Chromatography
author Serafim, Jéssica Aparecida [UNESP]
author_facet Serafim, Jéssica Aparecida [UNESP]
Silveira, Raiza Felismino [UNESP]
Vicente, Eduardo Festozo [UNESP]
author_role author
author2 Silveira, Raiza Felismino [UNESP]
Vicente, Eduardo Festozo [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Serafim, Jéssica Aparecida [UNESP]
Silveira, Raiza Felismino [UNESP]
Vicente, Eduardo Festozo [UNESP]
dc.subject.por.fl_str_mv Analysis
HPLC
RID
Ruminal fermentation
SCFA
UV-Vis
topic Analysis
HPLC
RID
Ruminal fermentation
SCFA
UV-Vis
description A rapid analytical method for simultaneous quantification of glucose (GLC) and seven short-chain fatty acids (SCFA) was developed for detection of ruminal fermentation parameters. The formic acid (FA), lactic acid (LA), acetic acid (AA), propionic acid (PA), butyric acid (BA), isovaleric acid (IVA), valeric acid (VA), and glucose were identified and quantified by high-performance liquid chromatography (HPLC) technique, using a reverse-phase column (C-18) and simultaneous detection in UV-Vis and Refraction Index (RID) detectors, linked in series. The method was validated following the requirements of selectivity, linearity, sensitivity, limit of detection, limit of quantification, accuracy, and precision. The developed method operates in isocratic and gradient modes, providing a better SCFA separation, which allows their determination with a high accuracy and repeatability. Therefore, this study provides a fast, reliable, accurate, straightforward, and efficient alternative method for analysis of ruminal fermentation parameters by liquid chromatography using both detectors simultaneously.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:23:32Z
2021-06-25T10:23:32Z
2021-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s12161-021-01990-w
Food Analytical Methods.
1936-976X
1936-9751
http://hdl.handle.net/11449/205918
10.1007/s12161-021-01990-w
2-s2.0-85101211661
url http://dx.doi.org/10.1007/s12161-021-01990-w
http://hdl.handle.net/11449/205918
identifier_str_mv Food Analytical Methods.
1936-976X
1936-9751
10.1007/s12161-021-01990-w
2-s2.0-85101211661
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Food Analytical Methods
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128759305338880

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