Occurrence and Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacterales Recovered From Chicken, Chicken Meat, and Human Infections in Sao Paulo State, Brazil

Detalhes bibliográficos
Autor(a) principal: Cardozo, Marita Vedovelli [UNESP]
Data de Publicação: 2021
Outros Autores: Liakopoulos, Apostolos, Brouwer, Michael, Kant, Arie, Pizauro, Lucas José Luduvério [UNESP], Borzi, Mariana Monezi [UNESP], Mevius, Dik, de Ávila, Fernando Antonio [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3389/fmicb.2021.628738
http://hdl.handle.net/11449/222376
Resumo: This study aimed to investigate the phylogenetic diversity and epidemiology of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae from chicken, chicken meat, and human clinical isolates in Sao Paolo, Brazil, and characterize their respective ESBL-encoding plasmids. Three hundred samples from chicken cloaca, chicken meat, and clinical isolates were phenotypically and genotypically assessed for ESBL resistance. Isolates were identified by MALDI TOF-MS and further characterized by MLST analysis and phylogenetic grouping. ESBL genes were characterized and their location was determined by I-Ceu-I-PFGE and Southern blot, conjugation, transformation, and PCR-based replicon typing experiments. Thirty-seven ESBL-producing isolates (28 E. coli and 9 K. pneumoniae) that were positive for the blaCTX–M–1 or blaCTX–M–2 gene groups were obtained. Two isolates were negative in the transformation assay, and the chromosomal location of the genes was deduced by Southern blot. The blaCTX–M genes identified were carried on plasmid replicon-types X1, HI2, N, FII-variants, I1 and R. The E. coli isolates belonged to nine sequence types, while the K. pneumoniae isolates belonged to four sequence types. The E. coli isolates belonged to phylotype classification groups A, B1, D, and F. This study demonstrated that isolates from cloacal swabs, chicken meat, and human feces had genetic diversity, with a high frequency of blaCTX–M–15 among chickens, chicken meat, and human feces. Thus, this reinforces the hypothesis that chickens, as well as their by-products, could be an important source of transmission for ESBL-producing pathogens to humans in South America.
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spelling Occurrence and Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacterales Recovered From Chicken, Chicken Meat, and Human Infections in Sao Paulo State, Brazilantibiotic resistanceextended spectrum beta lactamases (ESBLs)food chainplasmidspoultryThis study aimed to investigate the phylogenetic diversity and epidemiology of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae from chicken, chicken meat, and human clinical isolates in Sao Paolo, Brazil, and characterize their respective ESBL-encoding plasmids. Three hundred samples from chicken cloaca, chicken meat, and clinical isolates were phenotypically and genotypically assessed for ESBL resistance. Isolates were identified by MALDI TOF-MS and further characterized by MLST analysis and phylogenetic grouping. ESBL genes were characterized and their location was determined by I-Ceu-I-PFGE and Southern blot, conjugation, transformation, and PCR-based replicon typing experiments. Thirty-seven ESBL-producing isolates (28 E. coli and 9 K. pneumoniae) that were positive for the blaCTX–M–1 or blaCTX–M–2 gene groups were obtained. Two isolates were negative in the transformation assay, and the chromosomal location of the genes was deduced by Southern blot. The blaCTX–M genes identified were carried on plasmid replicon-types X1, HI2, N, FII-variants, I1 and R. The E. coli isolates belonged to nine sequence types, while the K. pneumoniae isolates belonged to four sequence types. The E. coli isolates belonged to phylotype classification groups A, B1, D, and F. This study demonstrated that isolates from cloacal swabs, chicken meat, and human feces had genetic diversity, with a high frequency of blaCTX–M–15 among chickens, chicken meat, and human feces. Thus, this reinforces the hypothesis that chickens, as well as their by-products, could be an important source of transmission for ESBL-producing pathogens to humans in South America.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Veterinary Pathology Faculty of Agricultural and Veterinary Sciences São Paulo State University (Unesp)Department of Bacteriology and Epidemiology Wageningen Bioveterinary Research Wageningen University and ResearchDepartment of Tecnology Faculty of Agricultural and Veterinary Sciences São Paulo State University (Unesp)Department of Veterinary Pathology Faculty of Agricultural and Veterinary Sciences São Paulo State University (Unesp)Department of Tecnology Faculty of Agricultural and Veterinary Sciences São Paulo State University (Unesp)FAPESP: 2013/18280-0FAPESP: 2015/10140-0Universidade Estadual Paulista (UNESP)Wageningen University and ResearchCardozo, Marita Vedovelli [UNESP]Liakopoulos, ApostolosBrouwer, MichaelKant, AriePizauro, Lucas José Luduvério [UNESP]Borzi, Mariana Monezi [UNESP]Mevius, Dikde Ávila, Fernando Antonio [UNESP]2022-04-28T19:44:17Z2022-04-28T19:44:17Z2021-06-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fmicb.2021.628738Frontiers in Microbiology, v. 12.1664-302Xhttp://hdl.handle.net/11449/22237610.3389/fmicb.2021.6287382-s2.0-85114522796Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Microbiologyinfo:eu-repo/semantics/openAccess2022-04-28T19:44:17Zoai:repositorio.unesp.br:11449/222376Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:26:30.871284Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Occurrence and Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacterales Recovered From Chicken, Chicken Meat, and Human Infections in Sao Paulo State, Brazil
title Occurrence and Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacterales Recovered From Chicken, Chicken Meat, and Human Infections in Sao Paulo State, Brazil
spellingShingle Occurrence and Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacterales Recovered From Chicken, Chicken Meat, and Human Infections in Sao Paulo State, Brazil
Cardozo, Marita Vedovelli [UNESP]
antibiotic resistance
extended spectrum beta lactamases (ESBLs)
food chain
plasmids
poultry
title_short Occurrence and Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacterales Recovered From Chicken, Chicken Meat, and Human Infections in Sao Paulo State, Brazil
title_full Occurrence and Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacterales Recovered From Chicken, Chicken Meat, and Human Infections in Sao Paulo State, Brazil
title_fullStr Occurrence and Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacterales Recovered From Chicken, Chicken Meat, and Human Infections in Sao Paulo State, Brazil
title_full_unstemmed Occurrence and Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacterales Recovered From Chicken, Chicken Meat, and Human Infections in Sao Paulo State, Brazil
title_sort Occurrence and Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacterales Recovered From Chicken, Chicken Meat, and Human Infections in Sao Paulo State, Brazil
author Cardozo, Marita Vedovelli [UNESP]
author_facet Cardozo, Marita Vedovelli [UNESP]
Liakopoulos, Apostolos
Brouwer, Michael
Kant, Arie
Pizauro, Lucas José Luduvério [UNESP]
Borzi, Mariana Monezi [UNESP]
Mevius, Dik
de Ávila, Fernando Antonio [UNESP]
author_role author
author2 Liakopoulos, Apostolos
Brouwer, Michael
Kant, Arie
Pizauro, Lucas José Luduvério [UNESP]
Borzi, Mariana Monezi [UNESP]
Mevius, Dik
de Ávila, Fernando Antonio [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Wageningen University and Research
dc.contributor.author.fl_str_mv Cardozo, Marita Vedovelli [UNESP]
Liakopoulos, Apostolos
Brouwer, Michael
Kant, Arie
Pizauro, Lucas José Luduvério [UNESP]
Borzi, Mariana Monezi [UNESP]
Mevius, Dik
de Ávila, Fernando Antonio [UNESP]
dc.subject.por.fl_str_mv antibiotic resistance
extended spectrum beta lactamases (ESBLs)
food chain
plasmids
poultry
topic antibiotic resistance
extended spectrum beta lactamases (ESBLs)
food chain
plasmids
poultry
description This study aimed to investigate the phylogenetic diversity and epidemiology of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae from chicken, chicken meat, and human clinical isolates in Sao Paolo, Brazil, and characterize their respective ESBL-encoding plasmids. Three hundred samples from chicken cloaca, chicken meat, and clinical isolates were phenotypically and genotypically assessed for ESBL resistance. Isolates were identified by MALDI TOF-MS and further characterized by MLST analysis and phylogenetic grouping. ESBL genes were characterized and their location was determined by I-Ceu-I-PFGE and Southern blot, conjugation, transformation, and PCR-based replicon typing experiments. Thirty-seven ESBL-producing isolates (28 E. coli and 9 K. pneumoniae) that were positive for the blaCTX–M–1 or blaCTX–M–2 gene groups were obtained. Two isolates were negative in the transformation assay, and the chromosomal location of the genes was deduced by Southern blot. The blaCTX–M genes identified were carried on plasmid replicon-types X1, HI2, N, FII-variants, I1 and R. The E. coli isolates belonged to nine sequence types, while the K. pneumoniae isolates belonged to four sequence types. The E. coli isolates belonged to phylotype classification groups A, B1, D, and F. This study demonstrated that isolates from cloacal swabs, chicken meat, and human feces had genetic diversity, with a high frequency of blaCTX–M–15 among chickens, chicken meat, and human feces. Thus, this reinforces the hypothesis that chickens, as well as their by-products, could be an important source of transmission for ESBL-producing pathogens to humans in South America.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-22
2022-04-28T19:44:17Z
2022-04-28T19:44:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3389/fmicb.2021.628738
Frontiers in Microbiology, v. 12.
1664-302X
http://hdl.handle.net/11449/222376
10.3389/fmicb.2021.628738
2-s2.0-85114522796
url http://dx.doi.org/10.3389/fmicb.2021.628738
http://hdl.handle.net/11449/222376
identifier_str_mv Frontiers in Microbiology, v. 12.
1664-302X
10.3389/fmicb.2021.628738
2-s2.0-85114522796
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers in Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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