RANK/RANKL/OPG expression in rapid maxillary expansion
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0103-6440201601116 http://hdl.handle.net/11449/179076 |
Resumo: | The aim of this study was to evaluate osteoclastogenesis signaling in midpalatal suture after rapid maxillary expansion (RME) in rats. Thirty male Wistar rats were randomly assigned to two groups with 15 animals each: control (C) and RME group. RME was performed by inserting a 1.5-mm-thick circular metal ring between the maxillary incisors. The animals were euthanized at 3, 7 and 10 days after RME. qRT-PCR was used to evaluate expression of Tnfsf11 (RANKL), Tnfrsf11a (RANK) and Tnfrsf11b (OPG). Data were submitted to statistical analysis using two-way ANOVA followed by Tukey test (α=0.05). There was an upregulation of RANK and RANKL genes at 7 and 10 days and an upregulation of the OPG gene at 3 and 7 days of healing. Interestingly, an increased in expression of all genes was observed over time in both RME and C groups. The RANKL/OPG ratio showed an increased signaling favoring bone resorption on RME compared to C at 3 and 7 days. Signaling against bone resorption was observed, as well as an upregulation of OPG gene expression in RME group, compared to C group at 10 days. The results of this study concluded that the RANK, RANK-L and OPG system participates in bone remodeling after RME. |
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Repositório Institucional da UNESP |
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spelling |
RANK/RANKL/OPG expression in rapid maxillary expansionBoneOPGRANKRANK-LRapid maxillary expansionThe aim of this study was to evaluate osteoclastogenesis signaling in midpalatal suture after rapid maxillary expansion (RME) in rats. Thirty male Wistar rats were randomly assigned to two groups with 15 animals each: control (C) and RME group. RME was performed by inserting a 1.5-mm-thick circular metal ring between the maxillary incisors. The animals were euthanized at 3, 7 and 10 days after RME. qRT-PCR was used to evaluate expression of Tnfsf11 (RANKL), Tnfrsf11a (RANK) and Tnfrsf11b (OPG). Data were submitted to statistical analysis using two-way ANOVA followed by Tukey test (α=0.05). There was an upregulation of RANK and RANKL genes at 7 and 10 days and an upregulation of the OPG gene at 3 and 7 days of healing. Interestingly, an increased in expression of all genes was observed over time in both RME and C groups. The RANKL/OPG ratio showed an increased signaling favoring bone resorption on RME compared to C at 3 and 7 days. Signaling against bone resorption was observed, as well as an upregulation of OPG gene expression in RME group, compared to C group at 10 days. The results of this study concluded that the RANK, RANK-L and OPG system participates in bone remodeling after RME.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Pediatric Clinics School of Dentistry of Ribeirão Preto USP - Universidade de São PauloDepartment of Histology School of Pharmacy of Ribeirão Preto USP - Universidade de São PauloDepartment of Histology UNESP - Universidade Estadual PaulistaDepartment of Histology UNESP - Universidade Estadual PaulistaUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Arnez, Maya Fernanda ManfrinRibeiro, Larissa Soares NogueiraBarretto, Gabriel DessottiMonteiro, Patrícia MariaErvolino, Edilson [UNESP]Stuani, Maria Bernadete Sasso2018-12-11T17:33:27Z2018-12-11T17:33:27Z2017-08-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article296-300application/pdfhttp://dx.doi.org/10.1590/0103-6440201601116Brazilian Dental Journal, v. 28, n. 3, p. 296-300, 2017.1806-47600103-6440http://hdl.handle.net/11449/17907610.1590/0103-6440201601116S0103-644020170003002962-s2.0-85026797884S0103-64402017000300296.pdf44080955173468460000-0003-4859-0583Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Dental Journal0,476info:eu-repo/semantics/openAccess2023-12-11T06:12:07Zoai:repositorio.unesp.br:11449/179076Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-11T06:12:07Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
RANK/RANKL/OPG expression in rapid maxillary expansion |
title |
RANK/RANKL/OPG expression in rapid maxillary expansion |
spellingShingle |
RANK/RANKL/OPG expression in rapid maxillary expansion Arnez, Maya Fernanda Manfrin Bone OPG RANK RANK-L Rapid maxillary expansion |
title_short |
RANK/RANKL/OPG expression in rapid maxillary expansion |
title_full |
RANK/RANKL/OPG expression in rapid maxillary expansion |
title_fullStr |
RANK/RANKL/OPG expression in rapid maxillary expansion |
title_full_unstemmed |
RANK/RANKL/OPG expression in rapid maxillary expansion |
title_sort |
RANK/RANKL/OPG expression in rapid maxillary expansion |
author |
Arnez, Maya Fernanda Manfrin |
author_facet |
Arnez, Maya Fernanda Manfrin Ribeiro, Larissa Soares Nogueira Barretto, Gabriel Dessotti Monteiro, Patrícia Maria Ervolino, Edilson [UNESP] Stuani, Maria Bernadete Sasso |
author_role |
author |
author2 |
Ribeiro, Larissa Soares Nogueira Barretto, Gabriel Dessotti Monteiro, Patrícia Maria Ervolino, Edilson [UNESP] Stuani, Maria Bernadete Sasso |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Arnez, Maya Fernanda Manfrin Ribeiro, Larissa Soares Nogueira Barretto, Gabriel Dessotti Monteiro, Patrícia Maria Ervolino, Edilson [UNESP] Stuani, Maria Bernadete Sasso |
dc.subject.por.fl_str_mv |
Bone OPG RANK RANK-L Rapid maxillary expansion |
topic |
Bone OPG RANK RANK-L Rapid maxillary expansion |
description |
The aim of this study was to evaluate osteoclastogenesis signaling in midpalatal suture after rapid maxillary expansion (RME) in rats. Thirty male Wistar rats were randomly assigned to two groups with 15 animals each: control (C) and RME group. RME was performed by inserting a 1.5-mm-thick circular metal ring between the maxillary incisors. The animals were euthanized at 3, 7 and 10 days after RME. qRT-PCR was used to evaluate expression of Tnfsf11 (RANKL), Tnfrsf11a (RANK) and Tnfrsf11b (OPG). Data were submitted to statistical analysis using two-way ANOVA followed by Tukey test (α=0.05). There was an upregulation of RANK and RANKL genes at 7 and 10 days and an upregulation of the OPG gene at 3 and 7 days of healing. Interestingly, an increased in expression of all genes was observed over time in both RME and C groups. The RANKL/OPG ratio showed an increased signaling favoring bone resorption on RME compared to C at 3 and 7 days. Signaling against bone resorption was observed, as well as an upregulation of OPG gene expression in RME group, compared to C group at 10 days. The results of this study concluded that the RANK, RANK-L and OPG system participates in bone remodeling after RME. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-02 2018-12-11T17:33:27Z 2018-12-11T17:33:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0103-6440201601116 Brazilian Dental Journal, v. 28, n. 3, p. 296-300, 2017. 1806-4760 0103-6440 http://hdl.handle.net/11449/179076 10.1590/0103-6440201601116 S0103-64402017000300296 2-s2.0-85026797884 S0103-64402017000300296.pdf 4408095517346846 0000-0003-4859-0583 |
url |
http://dx.doi.org/10.1590/0103-6440201601116 http://hdl.handle.net/11449/179076 |
identifier_str_mv |
Brazilian Dental Journal, v. 28, n. 3, p. 296-300, 2017. 1806-4760 0103-6440 10.1590/0103-6440201601116 S0103-64402017000300296 2-s2.0-85026797884 S0103-64402017000300296.pdf 4408095517346846 0000-0003-4859-0583 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Dental Journal 0,476 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
296-300 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965242789199872 |