Tobacco Smoke Induces Ventricular Remodeling Associated with an Increase in NADPH Oxidase Activity

Detalhes bibliográficos
Autor(a) principal: Rafacho, Bruna P. M. [UNESP]
Data de Publicação: 2011
Outros Autores: Gaiolla, Paula Schmidt Azevedo [UNESP], Polegato, Bertha Furlan [UNESP], Fernandes, Ana Angelica Henrique [UNESP], Bertoline, Maria A., Fernandes, Denise C., Chiuso-Minicucci, Fernanda [UNESP], Roscani, Meliza Goi [UNESP], dos Santos, Priscila P. [UNESP], Matsubara, Luiz Shiguero [UNESP], Matsubara, Beatriz Bojikian [UNESP], Laurindo, Francisco R. M., Paiva, Sergio Alberto Rupp de [UNESP], Zornoff, Leonardo Antonio Mamede [UNESP], Minicucci, Marcos Ferreira [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1159/000327957
http://hdl.handle.net/11449/18153
Resumo: Background: Recent studies have assessed the direct effects of smoking on cardiac remodeling and function. However, the mechanisms of these alterations remain unknown. The aim of this study was to investigate de role of cardiac NADPH oxidase and antioxidant enzyme system on ventricular remodeling induced by tobacco smoke. Methods: Male Wistar rats that weighed 200-230 g were divided into a control group (C) and an experimental group that was exposed to tobacco smoke for a period of two months (ETS). After the two-month exposure period, morphological, biochemical and functional analyses were performed. Results: The myocyte cross-sectional area and left ventricle end-diastolic dimension was increased 16.2% and 33.7%, respectively, in the ETS group. The interstitial collagen volume fraction was also higher in ETS group compared to the controls. In addition to these morphological changes, the ejection fraction and fractional shortening were decreased in the ETS group. Importantly, these alterations were related to augmented heart oxidative stress, which was characterized by an increase in NADPH oxidase activity, increased levels of lipid hydroperoxide and depletion of antioxidant enzymes (e.g., catalase, superoxide dismutase and glutathione peroxidase). In addition, cardiac levels of IFN-gamma, TNF-alpha and IL-10 were not different between the groups. Conclusion: Cardiac alterations that are induced by smoking are associated with increased NADPH oxidase activity, suggesting that this pathway plays a role in the ventricular remodeling induced by exposure to tobacco smoke. Copyright (C) 2011 S. Karger AG, Basel
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spelling Tobacco Smoke Induces Ventricular Remodeling Associated with an Increase in NADPH Oxidase ActivityOxidative stressTobacco smoke exposureCardiac remodelingBackground: Recent studies have assessed the direct effects of smoking on cardiac remodeling and function. However, the mechanisms of these alterations remain unknown. The aim of this study was to investigate de role of cardiac NADPH oxidase and antioxidant enzyme system on ventricular remodeling induced by tobacco smoke. Methods: Male Wistar rats that weighed 200-230 g were divided into a control group (C) and an experimental group that was exposed to tobacco smoke for a period of two months (ETS). After the two-month exposure period, morphological, biochemical and functional analyses were performed. Results: The myocyte cross-sectional area and left ventricle end-diastolic dimension was increased 16.2% and 33.7%, respectively, in the ETS group. The interstitial collagen volume fraction was also higher in ETS group compared to the controls. In addition to these morphological changes, the ejection fraction and fractional shortening were decreased in the ETS group. Importantly, these alterations were related to augmented heart oxidative stress, which was characterized by an increase in NADPH oxidase activity, increased levels of lipid hydroperoxide and depletion of antioxidant enzymes (e.g., catalase, superoxide dismutase and glutathione peroxidase). In addition, cardiac levels of IFN-gamma, TNF-alpha and IL-10 were not different between the groups. Conclusion: Cardiac alterations that are induced by smoking are associated with increased NADPH oxidase activity, suggesting that this pathway plays a role in the ventricular remodeling induced by exposure to tobacco smoke. Copyright (C) 2011 S. Karger AG, BaselCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Paulista, Botucatu Med Sch, Dept Internal Med, Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biol Sci, Dept Chem & Biochem, Botucatu, SP, BrazilUniv São Paulo, Sch Med, Heart Inst InCor, Vasc Biol Lab, São Paulo, BrazilSão Paulo State Univ, Inst Biosci, Dept Microbiol & Immunol, Botucatu, SP, BrazilUniv Estadual Paulista, Botucatu Med Sch, Dept Internal Med, Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biol Sci, Dept Chem & Biochem, Botucatu, SP, BrazilSão Paulo State Univ, Inst Biosci, Dept Microbiol & Immunol, Botucatu, SP, BrazilKargerUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Rafacho, Bruna P. M. [UNESP]Gaiolla, Paula Schmidt Azevedo [UNESP]Polegato, Bertha Furlan [UNESP]Fernandes, Ana Angelica Henrique [UNESP]Bertoline, Maria A.Fernandes, Denise C.Chiuso-Minicucci, Fernanda [UNESP]Roscani, Meliza Goi [UNESP]dos Santos, Priscila P. [UNESP]Matsubara, Luiz Shiguero [UNESP]Matsubara, Beatriz Bojikian [UNESP]Laurindo, Francisco R. M.Paiva, Sergio Alberto Rupp de [UNESP]Zornoff, Leonardo Antonio Mamede [UNESP]Minicucci, Marcos Ferreira [UNESP]2014-05-20T13:50:52Z2014-05-20T13:50:52Z2011-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article305-312application/pdfhttp://dx.doi.org/10.1159/000327957Cellular Physiology and Biochemistry. Basel: Karger, v. 27, n. 3-4, p. 305-312, 2011.1015-8987http://hdl.handle.net/11449/1815310.1159/000327957WOS:000290594900015WOS000290594900015.pdf630983513799876669909771223407955016839015394547121314080140264774387040344716730000-0002-5843-6232Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCellular Physiology and Biochemistry5.5001,561info:eu-repo/semantics/openAccess2024-08-14T17:36:53Zoai:repositorio.unesp.br:11449/18153Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:36:53Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Tobacco Smoke Induces Ventricular Remodeling Associated with an Increase in NADPH Oxidase Activity
title Tobacco Smoke Induces Ventricular Remodeling Associated with an Increase in NADPH Oxidase Activity
spellingShingle Tobacco Smoke Induces Ventricular Remodeling Associated with an Increase in NADPH Oxidase Activity
Rafacho, Bruna P. M. [UNESP]
Oxidative stress
Tobacco smoke exposure
Cardiac remodeling
title_short Tobacco Smoke Induces Ventricular Remodeling Associated with an Increase in NADPH Oxidase Activity
title_full Tobacco Smoke Induces Ventricular Remodeling Associated with an Increase in NADPH Oxidase Activity
title_fullStr Tobacco Smoke Induces Ventricular Remodeling Associated with an Increase in NADPH Oxidase Activity
title_full_unstemmed Tobacco Smoke Induces Ventricular Remodeling Associated with an Increase in NADPH Oxidase Activity
title_sort Tobacco Smoke Induces Ventricular Remodeling Associated with an Increase in NADPH Oxidase Activity
author Rafacho, Bruna P. M. [UNESP]
author_facet Rafacho, Bruna P. M. [UNESP]
Gaiolla, Paula Schmidt Azevedo [UNESP]
Polegato, Bertha Furlan [UNESP]
Fernandes, Ana Angelica Henrique [UNESP]
Bertoline, Maria A.
Fernandes, Denise C.
Chiuso-Minicucci, Fernanda [UNESP]
Roscani, Meliza Goi [UNESP]
dos Santos, Priscila P. [UNESP]
Matsubara, Luiz Shiguero [UNESP]
Matsubara, Beatriz Bojikian [UNESP]
Laurindo, Francisco R. M.
Paiva, Sergio Alberto Rupp de [UNESP]
Zornoff, Leonardo Antonio Mamede [UNESP]
Minicucci, Marcos Ferreira [UNESP]
author_role author
author2 Gaiolla, Paula Schmidt Azevedo [UNESP]
Polegato, Bertha Furlan [UNESP]
Fernandes, Ana Angelica Henrique [UNESP]
Bertoline, Maria A.
Fernandes, Denise C.
Chiuso-Minicucci, Fernanda [UNESP]
Roscani, Meliza Goi [UNESP]
dos Santos, Priscila P. [UNESP]
Matsubara, Luiz Shiguero [UNESP]
Matsubara, Beatriz Bojikian [UNESP]
Laurindo, Francisco R. M.
Paiva, Sergio Alberto Rupp de [UNESP]
Zornoff, Leonardo Antonio Mamede [UNESP]
Minicucci, Marcos Ferreira [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Rafacho, Bruna P. M. [UNESP]
Gaiolla, Paula Schmidt Azevedo [UNESP]
Polegato, Bertha Furlan [UNESP]
Fernandes, Ana Angelica Henrique [UNESP]
Bertoline, Maria A.
Fernandes, Denise C.
Chiuso-Minicucci, Fernanda [UNESP]
Roscani, Meliza Goi [UNESP]
dos Santos, Priscila P. [UNESP]
Matsubara, Luiz Shiguero [UNESP]
Matsubara, Beatriz Bojikian [UNESP]
Laurindo, Francisco R. M.
Paiva, Sergio Alberto Rupp de [UNESP]
Zornoff, Leonardo Antonio Mamede [UNESP]
Minicucci, Marcos Ferreira [UNESP]
dc.subject.por.fl_str_mv Oxidative stress
Tobacco smoke exposure
Cardiac remodeling
topic Oxidative stress
Tobacco smoke exposure
Cardiac remodeling
description Background: Recent studies have assessed the direct effects of smoking on cardiac remodeling and function. However, the mechanisms of these alterations remain unknown. The aim of this study was to investigate de role of cardiac NADPH oxidase and antioxidant enzyme system on ventricular remodeling induced by tobacco smoke. Methods: Male Wistar rats that weighed 200-230 g were divided into a control group (C) and an experimental group that was exposed to tobacco smoke for a period of two months (ETS). After the two-month exposure period, morphological, biochemical and functional analyses were performed. Results: The myocyte cross-sectional area and left ventricle end-diastolic dimension was increased 16.2% and 33.7%, respectively, in the ETS group. The interstitial collagen volume fraction was also higher in ETS group compared to the controls. In addition to these morphological changes, the ejection fraction and fractional shortening were decreased in the ETS group. Importantly, these alterations were related to augmented heart oxidative stress, which was characterized by an increase in NADPH oxidase activity, increased levels of lipid hydroperoxide and depletion of antioxidant enzymes (e.g., catalase, superoxide dismutase and glutathione peroxidase). In addition, cardiac levels of IFN-gamma, TNF-alpha and IL-10 were not different between the groups. Conclusion: Cardiac alterations that are induced by smoking are associated with increased NADPH oxidase activity, suggesting that this pathway plays a role in the ventricular remodeling induced by exposure to tobacco smoke. Copyright (C) 2011 S. Karger AG, Basel
publishDate 2011
dc.date.none.fl_str_mv 2011-01-01
2014-05-20T13:50:52Z
2014-05-20T13:50:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1159/000327957
Cellular Physiology and Biochemistry. Basel: Karger, v. 27, n. 3-4, p. 305-312, 2011.
1015-8987
http://hdl.handle.net/11449/18153
10.1159/000327957
WOS:000290594900015
WOS000290594900015.pdf
6309835137998766
6990977122340795
5016839015394547
1213140801402647
7438704034471673
0000-0002-5843-6232
url http://dx.doi.org/10.1159/000327957
http://hdl.handle.net/11449/18153
identifier_str_mv Cellular Physiology and Biochemistry. Basel: Karger, v. 27, n. 3-4, p. 305-312, 2011.
1015-8987
10.1159/000327957
WOS:000290594900015
WOS000290594900015.pdf
6309835137998766
6990977122340795
5016839015394547
1213140801402647
7438704034471673
0000-0002-5843-6232
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cellular Physiology and Biochemistry
5.500
1,561
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 305-312
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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