Tobacco Smoke Induces Ventricular Remodeling Associated with an Increase in NADPH Oxidase Activity
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1159/000327957 http://hdl.handle.net/11449/18153 |
Resumo: | Background: Recent studies have assessed the direct effects of smoking on cardiac remodeling and function. However, the mechanisms of these alterations remain unknown. The aim of this study was to investigate de role of cardiac NADPH oxidase and antioxidant enzyme system on ventricular remodeling induced by tobacco smoke. Methods: Male Wistar rats that weighed 200-230 g were divided into a control group (C) and an experimental group that was exposed to tobacco smoke for a period of two months (ETS). After the two-month exposure period, morphological, biochemical and functional analyses were performed. Results: The myocyte cross-sectional area and left ventricle end-diastolic dimension was increased 16.2% and 33.7%, respectively, in the ETS group. The interstitial collagen volume fraction was also higher in ETS group compared to the controls. In addition to these morphological changes, the ejection fraction and fractional shortening were decreased in the ETS group. Importantly, these alterations were related to augmented heart oxidative stress, which was characterized by an increase in NADPH oxidase activity, increased levels of lipid hydroperoxide and depletion of antioxidant enzymes (e.g., catalase, superoxide dismutase and glutathione peroxidase). In addition, cardiac levels of IFN-gamma, TNF-alpha and IL-10 were not different between the groups. Conclusion: Cardiac alterations that are induced by smoking are associated with increased NADPH oxidase activity, suggesting that this pathway plays a role in the ventricular remodeling induced by exposure to tobacco smoke. Copyright (C) 2011 S. Karger AG, Basel |
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Repositório Institucional da UNESP |
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Tobacco Smoke Induces Ventricular Remodeling Associated with an Increase in NADPH Oxidase ActivityOxidative stressTobacco smoke exposureCardiac remodelingBackground: Recent studies have assessed the direct effects of smoking on cardiac remodeling and function. However, the mechanisms of these alterations remain unknown. The aim of this study was to investigate de role of cardiac NADPH oxidase and antioxidant enzyme system on ventricular remodeling induced by tobacco smoke. Methods: Male Wistar rats that weighed 200-230 g were divided into a control group (C) and an experimental group that was exposed to tobacco smoke for a period of two months (ETS). After the two-month exposure period, morphological, biochemical and functional analyses were performed. Results: The myocyte cross-sectional area and left ventricle end-diastolic dimension was increased 16.2% and 33.7%, respectively, in the ETS group. The interstitial collagen volume fraction was also higher in ETS group compared to the controls. In addition to these morphological changes, the ejection fraction and fractional shortening were decreased in the ETS group. Importantly, these alterations were related to augmented heart oxidative stress, which was characterized by an increase in NADPH oxidase activity, increased levels of lipid hydroperoxide and depletion of antioxidant enzymes (e.g., catalase, superoxide dismutase and glutathione peroxidase). In addition, cardiac levels of IFN-gamma, TNF-alpha and IL-10 were not different between the groups. Conclusion: Cardiac alterations that are induced by smoking are associated with increased NADPH oxidase activity, suggesting that this pathway plays a role in the ventricular remodeling induced by exposure to tobacco smoke. Copyright (C) 2011 S. Karger AG, BaselCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Paulista, Botucatu Med Sch, Dept Internal Med, Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biol Sci, Dept Chem & Biochem, Botucatu, SP, BrazilUniv São Paulo, Sch Med, Heart Inst InCor, Vasc Biol Lab, São Paulo, BrazilSão Paulo State Univ, Inst Biosci, Dept Microbiol & Immunol, Botucatu, SP, BrazilUniv Estadual Paulista, Botucatu Med Sch, Dept Internal Med, Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biol Sci, Dept Chem & Biochem, Botucatu, SP, BrazilSão Paulo State Univ, Inst Biosci, Dept Microbiol & Immunol, Botucatu, SP, BrazilKargerUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Rafacho, Bruna P. M. [UNESP]Gaiolla, Paula Schmidt Azevedo [UNESP]Polegato, Bertha Furlan [UNESP]Fernandes, Ana Angelica Henrique [UNESP]Bertoline, Maria A.Fernandes, Denise C.Chiuso-Minicucci, Fernanda [UNESP]Roscani, Meliza Goi [UNESP]dos Santos, Priscila P. [UNESP]Matsubara, Luiz Shiguero [UNESP]Matsubara, Beatriz Bojikian [UNESP]Laurindo, Francisco R. M.Paiva, Sergio Alberto Rupp de [UNESP]Zornoff, Leonardo Antonio Mamede [UNESP]Minicucci, Marcos Ferreira [UNESP]2014-05-20T13:50:52Z2014-05-20T13:50:52Z2011-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article305-312application/pdfhttp://dx.doi.org/10.1159/000327957Cellular Physiology and Biochemistry. Basel: Karger, v. 27, n. 3-4, p. 305-312, 2011.1015-8987http://hdl.handle.net/11449/1815310.1159/000327957WOS:000290594900015WOS000290594900015.pdf630983513799876669909771223407955016839015394547121314080140264774387040344716730000-0002-5843-6232Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCellular Physiology and Biochemistry5.5001,561info:eu-repo/semantics/openAccess2024-08-14T17:36:53Zoai:repositorio.unesp.br:11449/18153Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:36:53Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Tobacco Smoke Induces Ventricular Remodeling Associated with an Increase in NADPH Oxidase Activity |
title |
Tobacco Smoke Induces Ventricular Remodeling Associated with an Increase in NADPH Oxidase Activity |
spellingShingle |
Tobacco Smoke Induces Ventricular Remodeling Associated with an Increase in NADPH Oxidase Activity Rafacho, Bruna P. M. [UNESP] Oxidative stress Tobacco smoke exposure Cardiac remodeling |
title_short |
Tobacco Smoke Induces Ventricular Remodeling Associated with an Increase in NADPH Oxidase Activity |
title_full |
Tobacco Smoke Induces Ventricular Remodeling Associated with an Increase in NADPH Oxidase Activity |
title_fullStr |
Tobacco Smoke Induces Ventricular Remodeling Associated with an Increase in NADPH Oxidase Activity |
title_full_unstemmed |
Tobacco Smoke Induces Ventricular Remodeling Associated with an Increase in NADPH Oxidase Activity |
title_sort |
Tobacco Smoke Induces Ventricular Remodeling Associated with an Increase in NADPH Oxidase Activity |
author |
Rafacho, Bruna P. M. [UNESP] |
author_facet |
Rafacho, Bruna P. M. [UNESP] Gaiolla, Paula Schmidt Azevedo [UNESP] Polegato, Bertha Furlan [UNESP] Fernandes, Ana Angelica Henrique [UNESP] Bertoline, Maria A. Fernandes, Denise C. Chiuso-Minicucci, Fernanda [UNESP] Roscani, Meliza Goi [UNESP] dos Santos, Priscila P. [UNESP] Matsubara, Luiz Shiguero [UNESP] Matsubara, Beatriz Bojikian [UNESP] Laurindo, Francisco R. M. Paiva, Sergio Alberto Rupp de [UNESP] Zornoff, Leonardo Antonio Mamede [UNESP] Minicucci, Marcos Ferreira [UNESP] |
author_role |
author |
author2 |
Gaiolla, Paula Schmidt Azevedo [UNESP] Polegato, Bertha Furlan [UNESP] Fernandes, Ana Angelica Henrique [UNESP] Bertoline, Maria A. Fernandes, Denise C. Chiuso-Minicucci, Fernanda [UNESP] Roscani, Meliza Goi [UNESP] dos Santos, Priscila P. [UNESP] Matsubara, Luiz Shiguero [UNESP] Matsubara, Beatriz Bojikian [UNESP] Laurindo, Francisco R. M. Paiva, Sergio Alberto Rupp de [UNESP] Zornoff, Leonardo Antonio Mamede [UNESP] Minicucci, Marcos Ferreira [UNESP] |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Rafacho, Bruna P. M. [UNESP] Gaiolla, Paula Schmidt Azevedo [UNESP] Polegato, Bertha Furlan [UNESP] Fernandes, Ana Angelica Henrique [UNESP] Bertoline, Maria A. Fernandes, Denise C. Chiuso-Minicucci, Fernanda [UNESP] Roscani, Meliza Goi [UNESP] dos Santos, Priscila P. [UNESP] Matsubara, Luiz Shiguero [UNESP] Matsubara, Beatriz Bojikian [UNESP] Laurindo, Francisco R. M. Paiva, Sergio Alberto Rupp de [UNESP] Zornoff, Leonardo Antonio Mamede [UNESP] Minicucci, Marcos Ferreira [UNESP] |
dc.subject.por.fl_str_mv |
Oxidative stress Tobacco smoke exposure Cardiac remodeling |
topic |
Oxidative stress Tobacco smoke exposure Cardiac remodeling |
description |
Background: Recent studies have assessed the direct effects of smoking on cardiac remodeling and function. However, the mechanisms of these alterations remain unknown. The aim of this study was to investigate de role of cardiac NADPH oxidase and antioxidant enzyme system on ventricular remodeling induced by tobacco smoke. Methods: Male Wistar rats that weighed 200-230 g were divided into a control group (C) and an experimental group that was exposed to tobacco smoke for a period of two months (ETS). After the two-month exposure period, morphological, biochemical and functional analyses were performed. Results: The myocyte cross-sectional area and left ventricle end-diastolic dimension was increased 16.2% and 33.7%, respectively, in the ETS group. The interstitial collagen volume fraction was also higher in ETS group compared to the controls. In addition to these morphological changes, the ejection fraction and fractional shortening were decreased in the ETS group. Importantly, these alterations were related to augmented heart oxidative stress, which was characterized by an increase in NADPH oxidase activity, increased levels of lipid hydroperoxide and depletion of antioxidant enzymes (e.g., catalase, superoxide dismutase and glutathione peroxidase). In addition, cardiac levels of IFN-gamma, TNF-alpha and IL-10 were not different between the groups. Conclusion: Cardiac alterations that are induced by smoking are associated with increased NADPH oxidase activity, suggesting that this pathway plays a role in the ventricular remodeling induced by exposure to tobacco smoke. Copyright (C) 2011 S. Karger AG, Basel |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-01 2014-05-20T13:50:52Z 2014-05-20T13:50:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1159/000327957 Cellular Physiology and Biochemistry. Basel: Karger, v. 27, n. 3-4, p. 305-312, 2011. 1015-8987 http://hdl.handle.net/11449/18153 10.1159/000327957 WOS:000290594900015 WOS000290594900015.pdf 6309835137998766 6990977122340795 5016839015394547 1213140801402647 7438704034471673 0000-0002-5843-6232 |
url |
http://dx.doi.org/10.1159/000327957 http://hdl.handle.net/11449/18153 |
identifier_str_mv |
Cellular Physiology and Biochemistry. Basel: Karger, v. 27, n. 3-4, p. 305-312, 2011. 1015-8987 10.1159/000327957 WOS:000290594900015 WOS000290594900015.pdf 6309835137998766 6990977122340795 5016839015394547 1213140801402647 7438704034471673 0000-0002-5843-6232 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cellular Physiology and Biochemistry 5.500 1,561 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
305-312 application/pdf |
dc.publisher.none.fl_str_mv |
Karger |
publisher.none.fl_str_mv |
Karger |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128206610366464 |