Anticardiolipin antibodies from syphilis and systemic lupus erythematosus induce leakage in cardiolipin vesicles

Detalhes bibliográficos
Autor(a) principal: Cremião, M. P.D.
Data de Publicação: 1996
Outros Autores: Ceili, C. M., Chaimovich, H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/230892
Resumo: Anticardiolipin antibodies from sera of patients with systemic lupus erythematosus or syphilis induced leakage of entrapped carboxyfluorescein (CF) from cardiolipin (CL)/phosphatidylcholine(PC) vesicles prepared by sonication of equimolar mixtures of CL:PC. The sera dilution used here was 1:7500. IgG (5-20 μg/ml) from the same sera, not containing B2GPI, also produced a concentration-dependent leak. Vesicle leakage was inhibited by salt and was not detected with vesicles prepared exclusively with phosphatidylcholine. The demonstration of antibody-induced vesicle leakage offers a convenient system to investigate the mechanism of antibody-lipid binding as well as a potential diagnostic tool.
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spelling Anticardiolipin antibodies from syphilis and systemic lupus erythematosus induce leakage in cardiolipin vesiclesAnticardiolipin antibodiesCarboxyfluoresceinCardiolipinSyphilisSystemic lupus erythematosusVesiclesAnticardiolipin antibodies from sera of patients with systemic lupus erythematosus or syphilis induced leakage of entrapped carboxyfluorescein (CF) from cardiolipin (CL)/phosphatidylcholine(PC) vesicles prepared by sonication of equimolar mixtures of CL:PC. The sera dilution used here was 1:7500. IgG (5-20 μg/ml) from the same sera, not containing B2GPI, also produced a concentration-dependent leak. Vesicle leakage was inhibited by salt and was not detected with vesicles prepared exclusively with phosphatidylcholine. The demonstration of antibody-induced vesicle leakage offers a convenient system to investigate the mechanism of antibody-lipid binding as well as a potential diagnostic tool.Departamento de Fármacos Fac. de Ciências Farmaceuticas Universidade Estadual Pautista, 014801-902 Araraquara, SPDepartamento de Bioquímica Institute de Química Universidade de São Paulo, 05599-970 São Paulo, SPDepartamento de Bioquímica Institute de Química Universidade de São Paulo, Av Prof. Lineu Prestes, 748, 05599-970 São Paulo, SPUniversidade Estadual PautistaUniversidade de São Paulo (USP)Cremião, M. P.D.Ceili, C. M.Chaimovich, H.2022-04-29T08:42:29Z2022-04-29T08:42:29Z1996-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article489-494Brazilian Journal of Medical and Biological Research, v. 29, n. 4, p. 489-494, 1996.0100-879Xhttp://hdl.handle.net/11449/2308922-s2.0-0030124156Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccess2024-06-24T13:46:01Zoai:repositorio.unesp.br:11449/230892Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-24T13:46:01Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Anticardiolipin antibodies from syphilis and systemic lupus erythematosus induce leakage in cardiolipin vesicles
title Anticardiolipin antibodies from syphilis and systemic lupus erythematosus induce leakage in cardiolipin vesicles
spellingShingle Anticardiolipin antibodies from syphilis and systemic lupus erythematosus induce leakage in cardiolipin vesicles
Cremião, M. P.D.
Anticardiolipin antibodies
Carboxyfluorescein
Cardiolipin
Syphilis
Systemic lupus erythematosus
Vesicles
title_short Anticardiolipin antibodies from syphilis and systemic lupus erythematosus induce leakage in cardiolipin vesicles
title_full Anticardiolipin antibodies from syphilis and systemic lupus erythematosus induce leakage in cardiolipin vesicles
title_fullStr Anticardiolipin antibodies from syphilis and systemic lupus erythematosus induce leakage in cardiolipin vesicles
title_full_unstemmed Anticardiolipin antibodies from syphilis and systemic lupus erythematosus induce leakage in cardiolipin vesicles
title_sort Anticardiolipin antibodies from syphilis and systemic lupus erythematosus induce leakage in cardiolipin vesicles
author Cremião, M. P.D.
author_facet Cremião, M. P.D.
Ceili, C. M.
Chaimovich, H.
author_role author
author2 Ceili, C. M.
Chaimovich, H.
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Pautista
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Cremião, M. P.D.
Ceili, C. M.
Chaimovich, H.
dc.subject.por.fl_str_mv Anticardiolipin antibodies
Carboxyfluorescein
Cardiolipin
Syphilis
Systemic lupus erythematosus
Vesicles
topic Anticardiolipin antibodies
Carboxyfluorescein
Cardiolipin
Syphilis
Systemic lupus erythematosus
Vesicles
description Anticardiolipin antibodies from sera of patients with systemic lupus erythematosus or syphilis induced leakage of entrapped carboxyfluorescein (CF) from cardiolipin (CL)/phosphatidylcholine(PC) vesicles prepared by sonication of equimolar mixtures of CL:PC. The sera dilution used here was 1:7500. IgG (5-20 μg/ml) from the same sera, not containing B2GPI, also produced a concentration-dependent leak. Vesicle leakage was inhibited by salt and was not detected with vesicles prepared exclusively with phosphatidylcholine. The demonstration of antibody-induced vesicle leakage offers a convenient system to investigate the mechanism of antibody-lipid binding as well as a potential diagnostic tool.
publishDate 1996
dc.date.none.fl_str_mv 1996-12-01
2022-04-29T08:42:29Z
2022-04-29T08:42:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Brazilian Journal of Medical and Biological Research, v. 29, n. 4, p. 489-494, 1996.
0100-879X
http://hdl.handle.net/11449/230892
2-s2.0-0030124156
identifier_str_mv Brazilian Journal of Medical and Biological Research, v. 29, n. 4, p. 489-494, 1996.
0100-879X
2-s2.0-0030124156
url http://hdl.handle.net/11449/230892
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Medical and Biological Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 489-494
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1803045444444487680

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