Seroepidemiological analysis of toxoplasmosis in college students
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1678-9199-21-1 http://hdl.handle.net/11449/127267 |
Resumo: | Background Toxoplasmosis is a zoonosis caused by an obligate intracellular parasite, Toxoplasma gondii, which affects warm-blooded animals including humans. Its prevalence rates usually vary in different regions of the planet. Methods In this study, an analysis of the seroprevalence of toxoplasmosis among Brazilian students was proposed by means of IgG specific antibodies detection. The presence of anti-Toxoplasma gondiiantibodies by indirect fluorescent antibody test (IFAT) was also evaluated in order to compare it with enzyme-linked immunosorbent assay (ELISA) and to assess the use of 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) and o-phenylenediamine dihydrochloride chromogens. Results The IFAT method showed a seroprevalence of 22.3%. These results were similar to those obtained by ELISA (24.1%). The seroprevalence was directly estimated from the IgG avidity, which showed that in a sample of 112 students, three of them had acute infection, an incidence of 1.6% in the studied population. Conclusion In this study, the use of different chromogenic substrates in immunoenzymatic ELISA assays did not display different sensitivity in the detection of T. gondii-reagent serum. The extrapolation of results to this population must be carefully considered, since the investigation was conducted on a reduced sample. However, it allows us to emphasize the importance of careful and well prepared studies to identify risk factors for toxoplasmosis, to adopt preventive measures and to offer guidance to at-risk populations about the disease. |
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Seroepidemiological analysis of toxoplasmosis in college studentsToxoplasma gondiiToxoplasmosisEnzyme-linked immunosorbent assayIndirect fluorescent antibody testSeroprevalenceEpidemiologyBackground Toxoplasmosis is a zoonosis caused by an obligate intracellular parasite, Toxoplasma gondii, which affects warm-blooded animals including humans. Its prevalence rates usually vary in different regions of the planet. Methods In this study, an analysis of the seroprevalence of toxoplasmosis among Brazilian students was proposed by means of IgG specific antibodies detection. The presence of anti-Toxoplasma gondiiantibodies by indirect fluorescent antibody test (IFAT) was also evaluated in order to compare it with enzyme-linked immunosorbent assay (ELISA) and to assess the use of 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) and o-phenylenediamine dihydrochloride chromogens. Results The IFAT method showed a seroprevalence of 22.3%. These results were similar to those obtained by ELISA (24.1%). The seroprevalence was directly estimated from the IgG avidity, which showed that in a sample of 112 students, three of them had acute infection, an incidence of 1.6% in the studied population. Conclusion In this study, the use of different chromogenic substrates in immunoenzymatic ELISA assays did not display different sensitivity in the detection of T. gondii-reagent serum. The extrapolation of results to this population must be carefully considered, since the investigation was conducted on a reduced sample. However, it allows us to emphasize the importance of careful and well prepared studies to identify risk factors for toxoplasmosis, to adopt preventive measures and to offer guidance to at-risk populations about the disease.UNESP Department of Biological SciencesLondrina State University Department of Veterinary MedicineUniversity of São Paulo Institute for Technological ResearchUniversity of São Paulo Institute of Tropical Medicine of São PauloUniversity of São Paulo Ribeirão Preto Medical School Department of GeneticsUniversidade Estadual Paulista Laboratório de Genética e Terapia Celular Departamento de Ciências BiológicasUNESP Department of Biological SciencesUniversidade Estadual Paulista Laboratório de Genética e Terapia Celular Departamento de Ciências BiológicasCentro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESPUniversidade Estadual Paulista (Unesp)Londrina State University Department of Veterinary MedicineUniversity of São Paulo Institute for Technological ResearchUniversity of São Paulo Institute of Tropical Medicine of São PauloUniversity of São Paulo Ribeirão Preto Medical School Department of GeneticsRodrigues, Jaqueline PolizeliFrei, FernandoNavarro, Italmar TeodoricoSilva, Luciana PereiraMarcelino, Monica YonashiroAndrade-Junior, Heitor Franco deFaria, Carolina Arruda deSantos, MarisleneRibeiro-Paes, João Tadeu2015-08-21T17:54:46Z2015-08-21T17:54:46Z2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-7application/pdfhttp://dx.doi.org/10.1186/1678-9199-21-1Journal of Venomous Animals and Toxins including Tropical Diseases. Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP, v. 21, p. 1-7, 2015.1678-9199http://hdl.handle.net/11449/12726710.1186/1678-9199-21-1S1678-91992015000100311S1678-91992015000100311.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins including Tropical Diseases1.7820,573info:eu-repo/semantics/openAccess2023-10-06T06:01:24Zoai:repositorio.unesp.br:11449/127267Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:07:45.547366Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Seroepidemiological analysis of toxoplasmosis in college students |
title |
Seroepidemiological analysis of toxoplasmosis in college students |
spellingShingle |
Seroepidemiological analysis of toxoplasmosis in college students Rodrigues, Jaqueline Polizeli Toxoplasma gondii Toxoplasmosis Enzyme-linked immunosorbent assay Indirect fluorescent antibody test Seroprevalence Epidemiology |
title_short |
Seroepidemiological analysis of toxoplasmosis in college students |
title_full |
Seroepidemiological analysis of toxoplasmosis in college students |
title_fullStr |
Seroepidemiological analysis of toxoplasmosis in college students |
title_full_unstemmed |
Seroepidemiological analysis of toxoplasmosis in college students |
title_sort |
Seroepidemiological analysis of toxoplasmosis in college students |
author |
Rodrigues, Jaqueline Polizeli |
author_facet |
Rodrigues, Jaqueline Polizeli Frei, Fernando Navarro, Italmar Teodorico Silva, Luciana Pereira Marcelino, Monica Yonashiro Andrade-Junior, Heitor Franco de Faria, Carolina Arruda de Santos, Marislene Ribeiro-Paes, João Tadeu |
author_role |
author |
author2 |
Frei, Fernando Navarro, Italmar Teodorico Silva, Luciana Pereira Marcelino, Monica Yonashiro Andrade-Junior, Heitor Franco de Faria, Carolina Arruda de Santos, Marislene Ribeiro-Paes, João Tadeu |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Londrina State University Department of Veterinary Medicine University of São Paulo Institute for Technological Research University of São Paulo Institute of Tropical Medicine of São Paulo University of São Paulo Ribeirão Preto Medical School Department of Genetics |
dc.contributor.author.fl_str_mv |
Rodrigues, Jaqueline Polizeli Frei, Fernando Navarro, Italmar Teodorico Silva, Luciana Pereira Marcelino, Monica Yonashiro Andrade-Junior, Heitor Franco de Faria, Carolina Arruda de Santos, Marislene Ribeiro-Paes, João Tadeu |
dc.subject.por.fl_str_mv |
Toxoplasma gondii Toxoplasmosis Enzyme-linked immunosorbent assay Indirect fluorescent antibody test Seroprevalence Epidemiology |
topic |
Toxoplasma gondii Toxoplasmosis Enzyme-linked immunosorbent assay Indirect fluorescent antibody test Seroprevalence Epidemiology |
description |
Background Toxoplasmosis is a zoonosis caused by an obligate intracellular parasite, Toxoplasma gondii, which affects warm-blooded animals including humans. Its prevalence rates usually vary in different regions of the planet. Methods In this study, an analysis of the seroprevalence of toxoplasmosis among Brazilian students was proposed by means of IgG specific antibodies detection. The presence of anti-Toxoplasma gondiiantibodies by indirect fluorescent antibody test (IFAT) was also evaluated in order to compare it with enzyme-linked immunosorbent assay (ELISA) and to assess the use of 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) and o-phenylenediamine dihydrochloride chromogens. Results The IFAT method showed a seroprevalence of 22.3%. These results were similar to those obtained by ELISA (24.1%). The seroprevalence was directly estimated from the IgG avidity, which showed that in a sample of 112 students, three of them had acute infection, an incidence of 1.6% in the studied population. Conclusion In this study, the use of different chromogenic substrates in immunoenzymatic ELISA assays did not display different sensitivity in the detection of T. gondii-reagent serum. The extrapolation of results to this population must be carefully considered, since the investigation was conducted on a reduced sample. However, it allows us to emphasize the importance of careful and well prepared studies to identify risk factors for toxoplasmosis, to adopt preventive measures and to offer guidance to at-risk populations about the disease. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-08-21T17:54:46Z 2015-08-21T17:54:46Z 2015 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1678-9199-21-1 Journal of Venomous Animals and Toxins including Tropical Diseases. Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP, v. 21, p. 1-7, 2015. 1678-9199 http://hdl.handle.net/11449/127267 10.1186/1678-9199-21-1 S1678-91992015000100311 S1678-91992015000100311.pdf |
url |
http://dx.doi.org/10.1186/1678-9199-21-1 http://hdl.handle.net/11449/127267 |
identifier_str_mv |
Journal of Venomous Animals and Toxins including Tropical Diseases. Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP, v. 21, p. 1-7, 2015. 1678-9199 10.1186/1678-9199-21-1 S1678-91992015000100311 S1678-91992015000100311.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Venomous Animals and Toxins including Tropical Diseases 1.782 0,573 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-7 application/pdf |
dc.publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP |
publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128319225331712 |