Seroepidemiological analysis of toxoplasmosis in college students

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Jaqueline Polizeli
Data de Publicação: 2015
Outros Autores: Frei, Fernando, Navarro, Italmar Teodorico, Silva, Luciana Pereira, Marcelino, Monica Yonashiro, Andrade-Junior, Heitor Franco de, Faria, Carolina Arruda de, Santos, Marislene, Ribeiro-Paes, João Tadeu
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1186/1678-9199-21-1
http://hdl.handle.net/11449/127267
Resumo: Background Toxoplasmosis is a zoonosis caused by an obligate intracellular parasite, Toxoplasma gondii, which affects warm-blooded animals including humans. Its prevalence rates usually vary in different regions of the planet. Methods In this study, an analysis of the seroprevalence of toxoplasmosis among Brazilian students was proposed by means of IgG specific antibodies detection. The presence of anti-Toxoplasma gondiiantibodies by indirect fluorescent antibody test (IFAT) was also evaluated in order to compare it with enzyme-linked immunosorbent assay (ELISA) and to assess the use of 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) and o-phenylenediamine dihydrochloride chromogens. Results The IFAT method showed a seroprevalence of 22.3%. These results were similar to those obtained by ELISA (24.1%). The seroprevalence was directly estimated from the IgG avidity, which showed that in a sample of 112 students, three of them had acute infection, an incidence of 1.6% in the studied population. Conclusion In this study, the use of different chromogenic substrates in immunoenzymatic ELISA assays did not display different sensitivity in the detection of T. gondii-reagent serum. The extrapolation of results to this population must be carefully considered, since the investigation was conducted on a reduced sample. However, it allows us to emphasize the importance of careful and well prepared studies to identify risk factors for toxoplasmosis, to adopt preventive measures and to offer guidance to at-risk populations about the disease.
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spelling Seroepidemiological analysis of toxoplasmosis in college studentsToxoplasma gondiiToxoplasmosisEnzyme-linked immunosorbent assayIndirect fluorescent antibody testSeroprevalenceEpidemiologyBackground Toxoplasmosis is a zoonosis caused by an obligate intracellular parasite, Toxoplasma gondii, which affects warm-blooded animals including humans. Its prevalence rates usually vary in different regions of the planet. Methods In this study, an analysis of the seroprevalence of toxoplasmosis among Brazilian students was proposed by means of IgG specific antibodies detection. The presence of anti-Toxoplasma gondiiantibodies by indirect fluorescent antibody test (IFAT) was also evaluated in order to compare it with enzyme-linked immunosorbent assay (ELISA) and to assess the use of 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) and o-phenylenediamine dihydrochloride chromogens. Results The IFAT method showed a seroprevalence of 22.3%. These results were similar to those obtained by ELISA (24.1%). The seroprevalence was directly estimated from the IgG avidity, which showed that in a sample of 112 students, three of them had acute infection, an incidence of 1.6% in the studied population. Conclusion In this study, the use of different chromogenic substrates in immunoenzymatic ELISA assays did not display different sensitivity in the detection of T. gondii-reagent serum. The extrapolation of results to this population must be carefully considered, since the investigation was conducted on a reduced sample. However, it allows us to emphasize the importance of careful and well prepared studies to identify risk factors for toxoplasmosis, to adopt preventive measures and to offer guidance to at-risk populations about the disease.UNESP Department of Biological SciencesLondrina State University Department of Veterinary MedicineUniversity of São Paulo Institute for Technological ResearchUniversity of São Paulo Institute of Tropical Medicine of São PauloUniversity of São Paulo Ribeirão Preto Medical School Department of GeneticsUniversidade Estadual Paulista Laboratório de Genética e Terapia Celular Departamento de Ciências BiológicasUNESP Department of Biological SciencesUniversidade Estadual Paulista Laboratório de Genética e Terapia Celular Departamento de Ciências BiológicasCentro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESPUniversidade Estadual Paulista (Unesp)Londrina State University Department of Veterinary MedicineUniversity of São Paulo Institute for Technological ResearchUniversity of São Paulo Institute of Tropical Medicine of São PauloUniversity of São Paulo Ribeirão Preto Medical School Department of GeneticsRodrigues, Jaqueline PolizeliFrei, FernandoNavarro, Italmar TeodoricoSilva, Luciana PereiraMarcelino, Monica YonashiroAndrade-Junior, Heitor Franco deFaria, Carolina Arruda deSantos, MarisleneRibeiro-Paes, João Tadeu2015-08-21T17:54:46Z2015-08-21T17:54:46Z2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-7application/pdfhttp://dx.doi.org/10.1186/1678-9199-21-1Journal of Venomous Animals and Toxins including Tropical Diseases. Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP, v. 21, p. 1-7, 2015.1678-9199http://hdl.handle.net/11449/12726710.1186/1678-9199-21-1S1678-91992015000100311S1678-91992015000100311.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins including Tropical Diseases1.7820,573info:eu-repo/semantics/openAccess2023-10-06T06:01:24Zoai:repositorio.unesp.br:11449/127267Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:07:45.547366Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Seroepidemiological analysis of toxoplasmosis in college students
title Seroepidemiological analysis of toxoplasmosis in college students
spellingShingle Seroepidemiological analysis of toxoplasmosis in college students
Rodrigues, Jaqueline Polizeli
Toxoplasma gondii
Toxoplasmosis
Enzyme-linked immunosorbent assay
Indirect fluorescent antibody test
Seroprevalence
Epidemiology
title_short Seroepidemiological analysis of toxoplasmosis in college students
title_full Seroepidemiological analysis of toxoplasmosis in college students
title_fullStr Seroepidemiological analysis of toxoplasmosis in college students
title_full_unstemmed Seroepidemiological analysis of toxoplasmosis in college students
title_sort Seroepidemiological analysis of toxoplasmosis in college students
author Rodrigues, Jaqueline Polizeli
author_facet Rodrigues, Jaqueline Polizeli
Frei, Fernando
Navarro, Italmar Teodorico
Silva, Luciana Pereira
Marcelino, Monica Yonashiro
Andrade-Junior, Heitor Franco de
Faria, Carolina Arruda de
Santos, Marislene
Ribeiro-Paes, João Tadeu
author_role author
author2 Frei, Fernando
Navarro, Italmar Teodorico
Silva, Luciana Pereira
Marcelino, Monica Yonashiro
Andrade-Junior, Heitor Franco de
Faria, Carolina Arruda de
Santos, Marislene
Ribeiro-Paes, João Tadeu
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Londrina State University Department of Veterinary Medicine
University of São Paulo Institute for Technological Research
University of São Paulo Institute of Tropical Medicine of São Paulo
University of São Paulo Ribeirão Preto Medical School Department of Genetics
dc.contributor.author.fl_str_mv Rodrigues, Jaqueline Polizeli
Frei, Fernando
Navarro, Italmar Teodorico
Silva, Luciana Pereira
Marcelino, Monica Yonashiro
Andrade-Junior, Heitor Franco de
Faria, Carolina Arruda de
Santos, Marislene
Ribeiro-Paes, João Tadeu
dc.subject.por.fl_str_mv Toxoplasma gondii
Toxoplasmosis
Enzyme-linked immunosorbent assay
Indirect fluorescent antibody test
Seroprevalence
Epidemiology
topic Toxoplasma gondii
Toxoplasmosis
Enzyme-linked immunosorbent assay
Indirect fluorescent antibody test
Seroprevalence
Epidemiology
description Background Toxoplasmosis is a zoonosis caused by an obligate intracellular parasite, Toxoplasma gondii, which affects warm-blooded animals including humans. Its prevalence rates usually vary in different regions of the planet. Methods In this study, an analysis of the seroprevalence of toxoplasmosis among Brazilian students was proposed by means of IgG specific antibodies detection. The presence of anti-Toxoplasma gondiiantibodies by indirect fluorescent antibody test (IFAT) was also evaluated in order to compare it with enzyme-linked immunosorbent assay (ELISA) and to assess the use of 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) and o-phenylenediamine dihydrochloride chromogens. Results The IFAT method showed a seroprevalence of 22.3%. These results were similar to those obtained by ELISA (24.1%). The seroprevalence was directly estimated from the IgG avidity, which showed that in a sample of 112 students, three of them had acute infection, an incidence of 1.6% in the studied population. Conclusion In this study, the use of different chromogenic substrates in immunoenzymatic ELISA assays did not display different sensitivity in the detection of T. gondii-reagent serum. The extrapolation of results to this population must be carefully considered, since the investigation was conducted on a reduced sample. However, it allows us to emphasize the importance of careful and well prepared studies to identify risk factors for toxoplasmosis, to adopt preventive measures and to offer guidance to at-risk populations about the disease.
publishDate 2015
dc.date.none.fl_str_mv 2015-08-21T17:54:46Z
2015-08-21T17:54:46Z
2015
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/1678-9199-21-1
Journal of Venomous Animals and Toxins including Tropical Diseases. Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP, v. 21, p. 1-7, 2015.
1678-9199
http://hdl.handle.net/11449/127267
10.1186/1678-9199-21-1
S1678-91992015000100311
S1678-91992015000100311.pdf
url http://dx.doi.org/10.1186/1678-9199-21-1
http://hdl.handle.net/11449/127267
identifier_str_mv Journal of Venomous Animals and Toxins including Tropical Diseases. Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP, v. 21, p. 1-7, 2015.
1678-9199
10.1186/1678-9199-21-1
S1678-91992015000100311
S1678-91992015000100311.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Venomous Animals and Toxins including Tropical Diseases
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dc.format.none.fl_str_mv 1-7
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dc.publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP
publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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