Two Novel Mutations Associated with Polymyxin-B Resistance in a Pandemic Lineage of Uropathogenic Escherichia coli of the Sequence Type 69
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1159/000517817 http://hdl.handle.net/11449/233382 |
Resumo: | Background: Uropathogenic Escherichia coli (UPEC) are frequent pathogens worldwide, impacting on the morbidity and economic costs associated with antimicrobial treatment. Objectives: We report two novel mutations associated with polymyxin-B resistance in an UPEC isolate collected in 2019. Methods: Isolate was submitted to antimicrobial susceptibility testing including broth microdilution for polymyxin B. Whole genome was sequenced and analyzed. Results: Polymyxin-B total inhibition occurred at 16 mg/L (resistant). UPEC isolate was assigned to the phylogroup D, serotype O117:H4, and Sequence Type 69. mcr genes were not detected, but two novel mutations in the pmrA/basS (A80S) and pmrB/basR (D149N) genes were identified. Conclusions: The occurrence of non-mcr polymyxin resistance in E. coli from extraintestinal infections underscores the need of a continuous surveillance of this evolving pathogen. |
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Repositório Institucional da UNESP |
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spelling |
Two Novel Mutations Associated with Polymyxin-B Resistance in a Pandemic Lineage of Uropathogenic Escherichia coli of the Sequence Type 69Antimicrobial resistanceEscherichia coliPolymyxin BUropathogenic Escherichia coliWhole-genome sequencingBackground: Uropathogenic Escherichia coli (UPEC) are frequent pathogens worldwide, impacting on the morbidity and economic costs associated with antimicrobial treatment. Objectives: We report two novel mutations associated with polymyxin-B resistance in an UPEC isolate collected in 2019. Methods: Isolate was submitted to antimicrobial susceptibility testing including broth microdilution for polymyxin B. Whole genome was sequenced and analyzed. Results: Polymyxin-B total inhibition occurred at 16 mg/L (resistant). UPEC isolate was assigned to the phylogroup D, serotype O117:H4, and Sequence Type 69. mcr genes were not detected, but two novel mutations in the pmrA/basS (A80S) and pmrB/basR (D149N) genes were identified. Conclusions: The occurrence of non-mcr polymyxin resistance in E. coli from extraintestinal infections underscores the need of a continuous surveillance of this evolving pathogen.Instituto Adolfo LutzInstituto de Biociências de Botucatu Universidade Estadual Paulista júlio de Mesquita FilhoFaculdade de Medicina Veterinária e Zootecnia Universidade de São PauloInstituto de Biociências de Botucatu Universidade Estadual Paulista júlio de Mesquita FilhoInstituto Adolfo LutzUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Dos Santos, Carla AdrianaHernandes, Rodrigo Tavanelli [UNESP]Cunha, Marcos Paulo VieiraNagamori, Filipe OnishiGonçalves, Claudia ReginaSacchi, Claudio TavaresTiba-Casas, Monique RibeiroCamargo, Carlos Henrique2022-05-01T08:15:13Z2022-05-01T08:15:13Z2021-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article92-98http://dx.doi.org/10.1159/000517817Chemotherapy, v. 66, n. 3, p. 92-98, 2021.1421-97940009-3157http://hdl.handle.net/11449/23338210.1159/0005178172-s2.0-85112397154Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemotherapyinfo:eu-repo/semantics/openAccess2022-05-01T08:15:13Zoai:repositorio.unesp.br:11449/233382Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:30:33.500443Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Two Novel Mutations Associated with Polymyxin-B Resistance in a Pandemic Lineage of Uropathogenic Escherichia coli of the Sequence Type 69 |
title |
Two Novel Mutations Associated with Polymyxin-B Resistance in a Pandemic Lineage of Uropathogenic Escherichia coli of the Sequence Type 69 |
spellingShingle |
Two Novel Mutations Associated with Polymyxin-B Resistance in a Pandemic Lineage of Uropathogenic Escherichia coli of the Sequence Type 69 Dos Santos, Carla Adriana Antimicrobial resistance Escherichia coli Polymyxin B Uropathogenic Escherichia coli Whole-genome sequencing |
title_short |
Two Novel Mutations Associated with Polymyxin-B Resistance in a Pandemic Lineage of Uropathogenic Escherichia coli of the Sequence Type 69 |
title_full |
Two Novel Mutations Associated with Polymyxin-B Resistance in a Pandemic Lineage of Uropathogenic Escherichia coli of the Sequence Type 69 |
title_fullStr |
Two Novel Mutations Associated with Polymyxin-B Resistance in a Pandemic Lineage of Uropathogenic Escherichia coli of the Sequence Type 69 |
title_full_unstemmed |
Two Novel Mutations Associated with Polymyxin-B Resistance in a Pandemic Lineage of Uropathogenic Escherichia coli of the Sequence Type 69 |
title_sort |
Two Novel Mutations Associated with Polymyxin-B Resistance in a Pandemic Lineage of Uropathogenic Escherichia coli of the Sequence Type 69 |
author |
Dos Santos, Carla Adriana |
author_facet |
Dos Santos, Carla Adriana Hernandes, Rodrigo Tavanelli [UNESP] Cunha, Marcos Paulo Vieira Nagamori, Filipe Onishi Gonçalves, Claudia Regina Sacchi, Claudio Tavares Tiba-Casas, Monique Ribeiro Camargo, Carlos Henrique |
author_role |
author |
author2 |
Hernandes, Rodrigo Tavanelli [UNESP] Cunha, Marcos Paulo Vieira Nagamori, Filipe Onishi Gonçalves, Claudia Regina Sacchi, Claudio Tavares Tiba-Casas, Monique Ribeiro Camargo, Carlos Henrique |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Instituto Adolfo Lutz Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Dos Santos, Carla Adriana Hernandes, Rodrigo Tavanelli [UNESP] Cunha, Marcos Paulo Vieira Nagamori, Filipe Onishi Gonçalves, Claudia Regina Sacchi, Claudio Tavares Tiba-Casas, Monique Ribeiro Camargo, Carlos Henrique |
dc.subject.por.fl_str_mv |
Antimicrobial resistance Escherichia coli Polymyxin B Uropathogenic Escherichia coli Whole-genome sequencing |
topic |
Antimicrobial resistance Escherichia coli Polymyxin B Uropathogenic Escherichia coli Whole-genome sequencing |
description |
Background: Uropathogenic Escherichia coli (UPEC) are frequent pathogens worldwide, impacting on the morbidity and economic costs associated with antimicrobial treatment. Objectives: We report two novel mutations associated with polymyxin-B resistance in an UPEC isolate collected in 2019. Methods: Isolate was submitted to antimicrobial susceptibility testing including broth microdilution for polymyxin B. Whole genome was sequenced and analyzed. Results: Polymyxin-B total inhibition occurred at 16 mg/L (resistant). UPEC isolate was assigned to the phylogroup D, serotype O117:H4, and Sequence Type 69. mcr genes were not detected, but two novel mutations in the pmrA/basS (A80S) and pmrB/basR (D149N) genes were identified. Conclusions: The occurrence of non-mcr polymyxin resistance in E. coli from extraintestinal infections underscores the need of a continuous surveillance of this evolving pathogen. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-01 2022-05-01T08:15:13Z 2022-05-01T08:15:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1159/000517817 Chemotherapy, v. 66, n. 3, p. 92-98, 2021. 1421-9794 0009-3157 http://hdl.handle.net/11449/233382 10.1159/000517817 2-s2.0-85112397154 |
url |
http://dx.doi.org/10.1159/000517817 http://hdl.handle.net/11449/233382 |
identifier_str_mv |
Chemotherapy, v. 66, n. 3, p. 92-98, 2021. 1421-9794 0009-3157 10.1159/000517817 2-s2.0-85112397154 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Chemotherapy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
92-98 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129078702637056 |