Two Novel Mutations Associated with Polymyxin-B Resistance in a Pandemic Lineage of Uropathogenic Escherichia coli of the Sequence Type 69

Detalhes bibliográficos
Autor(a) principal: Dos Santos, Carla Adriana
Data de Publicação: 2021
Outros Autores: Hernandes, Rodrigo Tavanelli [UNESP], Cunha, Marcos Paulo Vieira, Nagamori, Filipe Onishi, Gonçalves, Claudia Regina, Sacchi, Claudio Tavares, Tiba-Casas, Monique Ribeiro, Camargo, Carlos Henrique
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1159/000517817
http://hdl.handle.net/11449/233382
Resumo: Background: Uropathogenic Escherichia coli (UPEC) are frequent pathogens worldwide, impacting on the morbidity and economic costs associated with antimicrobial treatment. Objectives: We report two novel mutations associated with polymyxin-B resistance in an UPEC isolate collected in 2019. Methods: Isolate was submitted to antimicrobial susceptibility testing including broth microdilution for polymyxin B. Whole genome was sequenced and analyzed. Results: Polymyxin-B total inhibition occurred at 16 mg/L (resistant). UPEC isolate was assigned to the phylogroup D, serotype O117:H4, and Sequence Type 69. mcr genes were not detected, but two novel mutations in the pmrA/basS (A80S) and pmrB/basR (D149N) genes were identified. Conclusions: The occurrence of non-mcr polymyxin resistance in E. coli from extraintestinal infections underscores the need of a continuous surveillance of this evolving pathogen.
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spelling Two Novel Mutations Associated with Polymyxin-B Resistance in a Pandemic Lineage of Uropathogenic Escherichia coli of the Sequence Type 69Antimicrobial resistanceEscherichia coliPolymyxin BUropathogenic Escherichia coliWhole-genome sequencingBackground: Uropathogenic Escherichia coli (UPEC) are frequent pathogens worldwide, impacting on the morbidity and economic costs associated with antimicrobial treatment. Objectives: We report two novel mutations associated with polymyxin-B resistance in an UPEC isolate collected in 2019. Methods: Isolate was submitted to antimicrobial susceptibility testing including broth microdilution for polymyxin B. Whole genome was sequenced and analyzed. Results: Polymyxin-B total inhibition occurred at 16 mg/L (resistant). UPEC isolate was assigned to the phylogroup D, serotype O117:H4, and Sequence Type 69. mcr genes were not detected, but two novel mutations in the pmrA/basS (A80S) and pmrB/basR (D149N) genes were identified. Conclusions: The occurrence of non-mcr polymyxin resistance in E. coli from extraintestinal infections underscores the need of a continuous surveillance of this evolving pathogen.Instituto Adolfo LutzInstituto de Biociências de Botucatu Universidade Estadual Paulista júlio de Mesquita FilhoFaculdade de Medicina Veterinária e Zootecnia Universidade de São PauloInstituto de Biociências de Botucatu Universidade Estadual Paulista júlio de Mesquita FilhoInstituto Adolfo LutzUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Dos Santos, Carla AdrianaHernandes, Rodrigo Tavanelli [UNESP]Cunha, Marcos Paulo VieiraNagamori, Filipe OnishiGonçalves, Claudia ReginaSacchi, Claudio TavaresTiba-Casas, Monique RibeiroCamargo, Carlos Henrique2022-05-01T08:15:13Z2022-05-01T08:15:13Z2021-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article92-98http://dx.doi.org/10.1159/000517817Chemotherapy, v. 66, n. 3, p. 92-98, 2021.1421-97940009-3157http://hdl.handle.net/11449/23338210.1159/0005178172-s2.0-85112397154Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemotherapyinfo:eu-repo/semantics/openAccess2022-05-01T08:15:13Zoai:repositorio.unesp.br:11449/233382Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:30:33.500443Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Two Novel Mutations Associated with Polymyxin-B Resistance in a Pandemic Lineage of Uropathogenic Escherichia coli of the Sequence Type 69
title Two Novel Mutations Associated with Polymyxin-B Resistance in a Pandemic Lineage of Uropathogenic Escherichia coli of the Sequence Type 69
spellingShingle Two Novel Mutations Associated with Polymyxin-B Resistance in a Pandemic Lineage of Uropathogenic Escherichia coli of the Sequence Type 69
Dos Santos, Carla Adriana
Antimicrobial resistance
Escherichia coli
Polymyxin B
Uropathogenic Escherichia coli
Whole-genome sequencing
title_short Two Novel Mutations Associated with Polymyxin-B Resistance in a Pandemic Lineage of Uropathogenic Escherichia coli of the Sequence Type 69
title_full Two Novel Mutations Associated with Polymyxin-B Resistance in a Pandemic Lineage of Uropathogenic Escherichia coli of the Sequence Type 69
title_fullStr Two Novel Mutations Associated with Polymyxin-B Resistance in a Pandemic Lineage of Uropathogenic Escherichia coli of the Sequence Type 69
title_full_unstemmed Two Novel Mutations Associated with Polymyxin-B Resistance in a Pandemic Lineage of Uropathogenic Escherichia coli of the Sequence Type 69
title_sort Two Novel Mutations Associated with Polymyxin-B Resistance in a Pandemic Lineage of Uropathogenic Escherichia coli of the Sequence Type 69
author Dos Santos, Carla Adriana
author_facet Dos Santos, Carla Adriana
Hernandes, Rodrigo Tavanelli [UNESP]
Cunha, Marcos Paulo Vieira
Nagamori, Filipe Onishi
Gonçalves, Claudia Regina
Sacchi, Claudio Tavares
Tiba-Casas, Monique Ribeiro
Camargo, Carlos Henrique
author_role author
author2 Hernandes, Rodrigo Tavanelli [UNESP]
Cunha, Marcos Paulo Vieira
Nagamori, Filipe Onishi
Gonçalves, Claudia Regina
Sacchi, Claudio Tavares
Tiba-Casas, Monique Ribeiro
Camargo, Carlos Henrique
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto Adolfo Lutz
Universidade Estadual Paulista (UNESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Dos Santos, Carla Adriana
Hernandes, Rodrigo Tavanelli [UNESP]
Cunha, Marcos Paulo Vieira
Nagamori, Filipe Onishi
Gonçalves, Claudia Regina
Sacchi, Claudio Tavares
Tiba-Casas, Monique Ribeiro
Camargo, Carlos Henrique
dc.subject.por.fl_str_mv Antimicrobial resistance
Escherichia coli
Polymyxin B
Uropathogenic Escherichia coli
Whole-genome sequencing
topic Antimicrobial resistance
Escherichia coli
Polymyxin B
Uropathogenic Escherichia coli
Whole-genome sequencing
description Background: Uropathogenic Escherichia coli (UPEC) are frequent pathogens worldwide, impacting on the morbidity and economic costs associated with antimicrobial treatment. Objectives: We report two novel mutations associated with polymyxin-B resistance in an UPEC isolate collected in 2019. Methods: Isolate was submitted to antimicrobial susceptibility testing including broth microdilution for polymyxin B. Whole genome was sequenced and analyzed. Results: Polymyxin-B total inhibition occurred at 16 mg/L (resistant). UPEC isolate was assigned to the phylogroup D, serotype O117:H4, and Sequence Type 69. mcr genes were not detected, but two novel mutations in the pmrA/basS (A80S) and pmrB/basR (D149N) genes were identified. Conclusions: The occurrence of non-mcr polymyxin resistance in E. coli from extraintestinal infections underscores the need of a continuous surveillance of this evolving pathogen.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-01
2022-05-01T08:15:13Z
2022-05-01T08:15:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1159/000517817
Chemotherapy, v. 66, n. 3, p. 92-98, 2021.
1421-9794
0009-3157
http://hdl.handle.net/11449/233382
10.1159/000517817
2-s2.0-85112397154
url http://dx.doi.org/10.1159/000517817
http://hdl.handle.net/11449/233382
identifier_str_mv Chemotherapy, v. 66, n. 3, p. 92-98, 2021.
1421-9794
0009-3157
10.1159/000517817
2-s2.0-85112397154
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Chemotherapy
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 92-98
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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