Chemical Constituents, Antimicrobial and Antibiofilm Activity of Lophocereus schottii Against Uropathogenic Escherichia coli
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/22311866.2022.2163690 http://hdl.handle.net/11449/246859 |
Resumo: | The objective of the present study was to determine the chemical profile, and the antimicrobial and antibiofilm effect of the Lophocereus schottii extract against uropathogenic Escherichia coli. A methanolic extract of L. schottii was analyzed by mass spectrometry (ESI-IT-MSn and APCI-IT-MSn) to determine the profile of bioactive compounds. The antimicrobial potential was assayed by the microdilution broth method. The effect of the extract on motility was also determined. Quorum sensing systems were evaluated using a biosensor with Chromobacterium violaceum. The prevention of biofilm formation and biofilm remotion were analyzed by the crystal violet method. The chemical analysis allows to identify 8 phenolic compounds, 2 alkaloids and 1 triterpene, most of these reported for the first time to this plant. L. schottii extract showed inhibitory and bactericidal activity against uropathogenic E. coli (MIC= 6.5 mg/mL and CMB= 13 mg/mL). In addition, the effect of L. schottii on motility was observed, since the concentration of 6.5 mg/mL reduced this process by 60%. With regard to the inhibition of the Quorum sensing system, L. schottii (6.5 mg/mL) reduce the production of violacein in 45%. The same concentration of the extract (6.5 mg/mL) resulted the most effective to prevent biofilm formation in 65%. Regarding the biofilm remotion the higher tested concentration (3 MIC) was the most effective, removing 84% of biomass. The results showed that L. schottii has a variety of bioactive compounds and antimicrobial and antibiofilm potential against uropathogenic E. coli. |
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Chemical Constituents, Antimicrobial and Antibiofilm Activity of Lophocereus schottii Against Uropathogenic Escherichia coliantibiofilmantimicrobialbioactive compoundsUropathogenic Escherichia coliThe objective of the present study was to determine the chemical profile, and the antimicrobial and antibiofilm effect of the Lophocereus schottii extract against uropathogenic Escherichia coli. A methanolic extract of L. schottii was analyzed by mass spectrometry (ESI-IT-MSn and APCI-IT-MSn) to determine the profile of bioactive compounds. The antimicrobial potential was assayed by the microdilution broth method. The effect of the extract on motility was also determined. Quorum sensing systems were evaluated using a biosensor with Chromobacterium violaceum. The prevention of biofilm formation and biofilm remotion were analyzed by the crystal violet method. The chemical analysis allows to identify 8 phenolic compounds, 2 alkaloids and 1 triterpene, most of these reported for the first time to this plant. L. schottii extract showed inhibitory and bactericidal activity against uropathogenic E. coli (MIC= 6.5 mg/mL and CMB= 13 mg/mL). In addition, the effect of L. schottii on motility was observed, since the concentration of 6.5 mg/mL reduced this process by 60%. With regard to the inhibition of the Quorum sensing system, L. schottii (6.5 mg/mL) reduce the production of violacein in 45%. The same concentration of the extract (6.5 mg/mL) resulted the most effective to prevent biofilm formation in 65%. Regarding the biofilm remotion the higher tested concentration (3 MIC) was the most effective, removing 84% of biomass. The results showed that L. schottii has a variety of bioactive compounds and antimicrobial and antibiofilm potential against uropathogenic E. coli.Coordinación de Tecnología de Alimentos de Origen Vegetal Centro de Investigación en Alimentación y Desarrollo, A.C. Carretera Gustavo Enrique Astiazarán Rosas No.42, SonoraDepartamento de Ciencias Químico Biológicas y Agropecuarias Universidad de Sonora, Unidad Regional Sur, Blvd. Lazaro Cárdenas 100, Colonia Francisco Villa, SonoraDepartamento de Ciencias Químico Biológicas y Agropecuarias Universidad de Sonora, Unidad Regional Norte, Avenida K S/N Col., Eleazar Ortiz CaborcaDepartamento de Ciencias Químico Biológicas Universidad de Sonora, Blvd. Luis Encinas y Rosales, SonoraUniversidade Estadual Paulista, Faculdade de Ciencias Farmaceuticas Campus de Araraquara, Rodovia Araraquara Jae, Km 01 - S/N, Campus Ville, SE o PauloDepartamento de Infectología Laboratorio de Bacteriología Hospital Infantil de México Federico GómezUniversidade Estadual Paulista, Faculdade de Ciencias Farmaceuticas Campus de Araraquara, Rodovia Araraquara Jae, Km 01 - S/N, Campus Ville, SE o PauloCentro de Investigación en Alimentación y DesarrolloUniversidad de SonoraUniversidade Estadual Paulista (UNESP)Hospital Infantil de México Federico GómezRoberto, Tapia-Rodríguez MelvinMax, Vidal-GutiérrezAlejandra, González-OrtizEnrique, Robles-Zepeda RamónWagner, Vilegas [UNESP]Fernando, Ayala-Zavala JesúsNorma, Velázquez-GuadarramaHeriberto, Torres-MorenoCésar, López-Romero Julio2023-07-29T12:52:33Z2023-07-29T12:52:33Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article461-473http://dx.doi.org/10.1080/22311866.2022.2163690Journal of Biologically Active Products from Nature, v. 12, n. 6, p. 461-473, 2022.2231-18742231-1866http://hdl.handle.net/11449/24685910.1080/22311866.2022.21636902-s2.0-85148438863Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Biologically Active Products from Natureinfo:eu-repo/semantics/openAccess2023-07-29T12:52:33Zoai:repositorio.unesp.br:11449/246859Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:47:23.199110Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Chemical Constituents, Antimicrobial and Antibiofilm Activity of Lophocereus schottii Against Uropathogenic Escherichia coli |
title |
Chemical Constituents, Antimicrobial and Antibiofilm Activity of Lophocereus schottii Against Uropathogenic Escherichia coli |
spellingShingle |
Chemical Constituents, Antimicrobial and Antibiofilm Activity of Lophocereus schottii Against Uropathogenic Escherichia coli Roberto, Tapia-Rodríguez Melvin antibiofilm antimicrobial bioactive compounds Uropathogenic Escherichia coli |
title_short |
Chemical Constituents, Antimicrobial and Antibiofilm Activity of Lophocereus schottii Against Uropathogenic Escherichia coli |
title_full |
Chemical Constituents, Antimicrobial and Antibiofilm Activity of Lophocereus schottii Against Uropathogenic Escherichia coli |
title_fullStr |
Chemical Constituents, Antimicrobial and Antibiofilm Activity of Lophocereus schottii Against Uropathogenic Escherichia coli |
title_full_unstemmed |
Chemical Constituents, Antimicrobial and Antibiofilm Activity of Lophocereus schottii Against Uropathogenic Escherichia coli |
title_sort |
Chemical Constituents, Antimicrobial and Antibiofilm Activity of Lophocereus schottii Against Uropathogenic Escherichia coli |
author |
Roberto, Tapia-Rodríguez Melvin |
author_facet |
Roberto, Tapia-Rodríguez Melvin Max, Vidal-Gutiérrez Alejandra, González-Ortiz Enrique, Robles-Zepeda Ramón Wagner, Vilegas [UNESP] Fernando, Ayala-Zavala Jesús Norma, Velázquez-Guadarrama Heriberto, Torres-Moreno César, López-Romero Julio |
author_role |
author |
author2 |
Max, Vidal-Gutiérrez Alejandra, González-Ortiz Enrique, Robles-Zepeda Ramón Wagner, Vilegas [UNESP] Fernando, Ayala-Zavala Jesús Norma, Velázquez-Guadarrama Heriberto, Torres-Moreno César, López-Romero Julio |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Centro de Investigación en Alimentación y Desarrollo Universidad de Sonora Universidade Estadual Paulista (UNESP) Hospital Infantil de México Federico Gómez |
dc.contributor.author.fl_str_mv |
Roberto, Tapia-Rodríguez Melvin Max, Vidal-Gutiérrez Alejandra, González-Ortiz Enrique, Robles-Zepeda Ramón Wagner, Vilegas [UNESP] Fernando, Ayala-Zavala Jesús Norma, Velázquez-Guadarrama Heriberto, Torres-Moreno César, López-Romero Julio |
dc.subject.por.fl_str_mv |
antibiofilm antimicrobial bioactive compounds Uropathogenic Escherichia coli |
topic |
antibiofilm antimicrobial bioactive compounds Uropathogenic Escherichia coli |
description |
The objective of the present study was to determine the chemical profile, and the antimicrobial and antibiofilm effect of the Lophocereus schottii extract against uropathogenic Escherichia coli. A methanolic extract of L. schottii was analyzed by mass spectrometry (ESI-IT-MSn and APCI-IT-MSn) to determine the profile of bioactive compounds. The antimicrobial potential was assayed by the microdilution broth method. The effect of the extract on motility was also determined. Quorum sensing systems were evaluated using a biosensor with Chromobacterium violaceum. The prevention of biofilm formation and biofilm remotion were analyzed by the crystal violet method. The chemical analysis allows to identify 8 phenolic compounds, 2 alkaloids and 1 triterpene, most of these reported for the first time to this plant. L. schottii extract showed inhibitory and bactericidal activity against uropathogenic E. coli (MIC= 6.5 mg/mL and CMB= 13 mg/mL). In addition, the effect of L. schottii on motility was observed, since the concentration of 6.5 mg/mL reduced this process by 60%. With regard to the inhibition of the Quorum sensing system, L. schottii (6.5 mg/mL) reduce the production of violacein in 45%. The same concentration of the extract (6.5 mg/mL) resulted the most effective to prevent biofilm formation in 65%. Regarding the biofilm remotion the higher tested concentration (3 MIC) was the most effective, removing 84% of biomass. The results showed that L. schottii has a variety of bioactive compounds and antimicrobial and antibiofilm potential against uropathogenic E. coli. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 2023-07-29T12:52:33Z 2023-07-29T12:52:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/22311866.2022.2163690 Journal of Biologically Active Products from Nature, v. 12, n. 6, p. 461-473, 2022. 2231-1874 2231-1866 http://hdl.handle.net/11449/246859 10.1080/22311866.2022.2163690 2-s2.0-85148438863 |
url |
http://dx.doi.org/10.1080/22311866.2022.2163690 http://hdl.handle.net/11449/246859 |
identifier_str_mv |
Journal of Biologically Active Products from Nature, v. 12, n. 6, p. 461-473, 2022. 2231-1874 2231-1866 10.1080/22311866.2022.2163690 2-s2.0-85148438863 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Biologically Active Products from Nature |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
461-473 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129551992094720 |