Chemical Constituents, Antimicrobial and Antibiofilm Activity of Lophocereus schottii Against Uropathogenic Escherichia coli

Detalhes bibliográficos
Autor(a) principal: Roberto, Tapia-Rodríguez Melvin
Data de Publicação: 2022
Outros Autores: Max, Vidal-Gutiérrez, Alejandra, González-Ortiz, Enrique, Robles-Zepeda Ramón, Wagner, Vilegas [UNESP], Fernando, Ayala-Zavala Jesús, Norma, Velázquez-Guadarrama, Heriberto, Torres-Moreno, César, López-Romero Julio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1080/22311866.2022.2163690
http://hdl.handle.net/11449/246859
Resumo: The objective of the present study was to determine the chemical profile, and the antimicrobial and antibiofilm effect of the Lophocereus schottii extract against uropathogenic Escherichia coli. A methanolic extract of L. schottii was analyzed by mass spectrometry (ESI-IT-MSn and APCI-IT-MSn) to determine the profile of bioactive compounds. The antimicrobial potential was assayed by the microdilution broth method. The effect of the extract on motility was also determined. Quorum sensing systems were evaluated using a biosensor with Chromobacterium violaceum. The prevention of biofilm formation and biofilm remotion were analyzed by the crystal violet method. The chemical analysis allows to identify 8 phenolic compounds, 2 alkaloids and 1 triterpene, most of these reported for the first time to this plant. L. schottii extract showed inhibitory and bactericidal activity against uropathogenic E. coli (MIC= 6.5 mg/mL and CMB= 13 mg/mL). In addition, the effect of L. schottii on motility was observed, since the concentration of 6.5 mg/mL reduced this process by 60%. With regard to the inhibition of the Quorum sensing system, L. schottii (6.5 mg/mL) reduce the production of violacein in 45%. The same concentration of the extract (6.5 mg/mL) resulted the most effective to prevent biofilm formation in 65%. Regarding the biofilm remotion the higher tested concentration (3 MIC) was the most effective, removing 84% of biomass. The results showed that L. schottii has a variety of bioactive compounds and antimicrobial and antibiofilm potential against uropathogenic E. coli.
id UNSP_d624e2da9d75946710fe968cf9840e06
oai_identifier_str oai:repositorio.unesp.br:11449/246859
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Chemical Constituents, Antimicrobial and Antibiofilm Activity of Lophocereus schottii Against Uropathogenic Escherichia coliantibiofilmantimicrobialbioactive compoundsUropathogenic Escherichia coliThe objective of the present study was to determine the chemical profile, and the antimicrobial and antibiofilm effect of the Lophocereus schottii extract against uropathogenic Escherichia coli. A methanolic extract of L. schottii was analyzed by mass spectrometry (ESI-IT-MSn and APCI-IT-MSn) to determine the profile of bioactive compounds. The antimicrobial potential was assayed by the microdilution broth method. The effect of the extract on motility was also determined. Quorum sensing systems were evaluated using a biosensor with Chromobacterium violaceum. The prevention of biofilm formation and biofilm remotion were analyzed by the crystal violet method. The chemical analysis allows to identify 8 phenolic compounds, 2 alkaloids and 1 triterpene, most of these reported for the first time to this plant. L. schottii extract showed inhibitory and bactericidal activity against uropathogenic E. coli (MIC= 6.5 mg/mL and CMB= 13 mg/mL). In addition, the effect of L. schottii on motility was observed, since the concentration of 6.5 mg/mL reduced this process by 60%. With regard to the inhibition of the Quorum sensing system, L. schottii (6.5 mg/mL) reduce the production of violacein in 45%. The same concentration of the extract (6.5 mg/mL) resulted the most effective to prevent biofilm formation in 65%. Regarding the biofilm remotion the higher tested concentration (3 MIC) was the most effective, removing 84% of biomass. The results showed that L. schottii has a variety of bioactive compounds and antimicrobial and antibiofilm potential against uropathogenic E. coli.Coordinación de Tecnología de Alimentos de Origen Vegetal Centro de Investigación en Alimentación y Desarrollo, A.C. Carretera Gustavo Enrique Astiazarán Rosas No.42, SonoraDepartamento de Ciencias Químico Biológicas y Agropecuarias Universidad de Sonora, Unidad Regional Sur, Blvd. Lazaro Cárdenas 100, Colonia Francisco Villa, SonoraDepartamento de Ciencias Químico Biológicas y Agropecuarias Universidad de Sonora, Unidad Regional Norte, Avenida K S/N Col., Eleazar Ortiz CaborcaDepartamento de Ciencias Químico Biológicas Universidad de Sonora, Blvd. Luis Encinas y Rosales, SonoraUniversidade Estadual Paulista, Faculdade de Ciencias Farmaceuticas Campus de Araraquara, Rodovia Araraquara Jae, Km 01 - S/N, Campus Ville, SE o PauloDepartamento de Infectología Laboratorio de Bacteriología Hospital Infantil de México Federico GómezUniversidade Estadual Paulista, Faculdade de Ciencias Farmaceuticas Campus de Araraquara, Rodovia Araraquara Jae, Km 01 - S/N, Campus Ville, SE o PauloCentro de Investigación en Alimentación y DesarrolloUniversidad de SonoraUniversidade Estadual Paulista (UNESP)Hospital Infantil de México Federico GómezRoberto, Tapia-Rodríguez MelvinMax, Vidal-GutiérrezAlejandra, González-OrtizEnrique, Robles-Zepeda RamónWagner, Vilegas [UNESP]Fernando, Ayala-Zavala JesúsNorma, Velázquez-GuadarramaHeriberto, Torres-MorenoCésar, López-Romero Julio2023-07-29T12:52:33Z2023-07-29T12:52:33Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article461-473http://dx.doi.org/10.1080/22311866.2022.2163690Journal of Biologically Active Products from Nature, v. 12, n. 6, p. 461-473, 2022.2231-18742231-1866http://hdl.handle.net/11449/24685910.1080/22311866.2022.21636902-s2.0-85148438863Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Biologically Active Products from Natureinfo:eu-repo/semantics/openAccess2023-07-29T12:52:33Zoai:repositorio.unesp.br:11449/246859Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:47:23.199110Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Chemical Constituents, Antimicrobial and Antibiofilm Activity of Lophocereus schottii Against Uropathogenic Escherichia coli
title Chemical Constituents, Antimicrobial and Antibiofilm Activity of Lophocereus schottii Against Uropathogenic Escherichia coli
spellingShingle Chemical Constituents, Antimicrobial and Antibiofilm Activity of Lophocereus schottii Against Uropathogenic Escherichia coli
Roberto, Tapia-Rodríguez Melvin
antibiofilm
antimicrobial
bioactive compounds
Uropathogenic Escherichia coli
title_short Chemical Constituents, Antimicrobial and Antibiofilm Activity of Lophocereus schottii Against Uropathogenic Escherichia coli
title_full Chemical Constituents, Antimicrobial and Antibiofilm Activity of Lophocereus schottii Against Uropathogenic Escherichia coli
title_fullStr Chemical Constituents, Antimicrobial and Antibiofilm Activity of Lophocereus schottii Against Uropathogenic Escherichia coli
title_full_unstemmed Chemical Constituents, Antimicrobial and Antibiofilm Activity of Lophocereus schottii Against Uropathogenic Escherichia coli
title_sort Chemical Constituents, Antimicrobial and Antibiofilm Activity of Lophocereus schottii Against Uropathogenic Escherichia coli
author Roberto, Tapia-Rodríguez Melvin
author_facet Roberto, Tapia-Rodríguez Melvin
Max, Vidal-Gutiérrez
Alejandra, González-Ortiz
Enrique, Robles-Zepeda Ramón
Wagner, Vilegas [UNESP]
Fernando, Ayala-Zavala Jesús
Norma, Velázquez-Guadarrama
Heriberto, Torres-Moreno
César, López-Romero Julio
author_role author
author2 Max, Vidal-Gutiérrez
Alejandra, González-Ortiz
Enrique, Robles-Zepeda Ramón
Wagner, Vilegas [UNESP]
Fernando, Ayala-Zavala Jesús
Norma, Velázquez-Guadarrama
Heriberto, Torres-Moreno
César, López-Romero Julio
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Centro de Investigación en Alimentación y Desarrollo
Universidad de Sonora
Universidade Estadual Paulista (UNESP)
Hospital Infantil de México Federico Gómez
dc.contributor.author.fl_str_mv Roberto, Tapia-Rodríguez Melvin
Max, Vidal-Gutiérrez
Alejandra, González-Ortiz
Enrique, Robles-Zepeda Ramón
Wagner, Vilegas [UNESP]
Fernando, Ayala-Zavala Jesús
Norma, Velázquez-Guadarrama
Heriberto, Torres-Moreno
César, López-Romero Julio
dc.subject.por.fl_str_mv antibiofilm
antimicrobial
bioactive compounds
Uropathogenic Escherichia coli
topic antibiofilm
antimicrobial
bioactive compounds
Uropathogenic Escherichia coli
description The objective of the present study was to determine the chemical profile, and the antimicrobial and antibiofilm effect of the Lophocereus schottii extract against uropathogenic Escherichia coli. A methanolic extract of L. schottii was analyzed by mass spectrometry (ESI-IT-MSn and APCI-IT-MSn) to determine the profile of bioactive compounds. The antimicrobial potential was assayed by the microdilution broth method. The effect of the extract on motility was also determined. Quorum sensing systems were evaluated using a biosensor with Chromobacterium violaceum. The prevention of biofilm formation and biofilm remotion were analyzed by the crystal violet method. The chemical analysis allows to identify 8 phenolic compounds, 2 alkaloids and 1 triterpene, most of these reported for the first time to this plant. L. schottii extract showed inhibitory and bactericidal activity against uropathogenic E. coli (MIC= 6.5 mg/mL and CMB= 13 mg/mL). In addition, the effect of L. schottii on motility was observed, since the concentration of 6.5 mg/mL reduced this process by 60%. With regard to the inhibition of the Quorum sensing system, L. schottii (6.5 mg/mL) reduce the production of violacein in 45%. The same concentration of the extract (6.5 mg/mL) resulted the most effective to prevent biofilm formation in 65%. Regarding the biofilm remotion the higher tested concentration (3 MIC) was the most effective, removing 84% of biomass. The results showed that L. schottii has a variety of bioactive compounds and antimicrobial and antibiofilm potential against uropathogenic E. coli.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
2023-07-29T12:52:33Z
2023-07-29T12:52:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1080/22311866.2022.2163690
Journal of Biologically Active Products from Nature, v. 12, n. 6, p. 461-473, 2022.
2231-1874
2231-1866
http://hdl.handle.net/11449/246859
10.1080/22311866.2022.2163690
2-s2.0-85148438863
url http://dx.doi.org/10.1080/22311866.2022.2163690
http://hdl.handle.net/11449/246859
identifier_str_mv Journal of Biologically Active Products from Nature, v. 12, n. 6, p. 461-473, 2022.
2231-1874
2231-1866
10.1080/22311866.2022.2163690
2-s2.0-85148438863
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Biologically Active Products from Nature
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 461-473
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129551992094720