Glucoregulatory and anti-inflammatory activities of peptide fractions separated by electrodialysis with ultrafiltration membranes from salmon protein hydrolysate and identification of four novel glucoregulatory peptides
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/membranes11070528 http://hdl.handle.net/11449/222057 |
Resumo: | Natural bioactive peptides are suitable candidates for preventing the development of Type 2 diabetes (T2D), by reducing the various risk factors. The aim of this study was to concentrate glucoregulatory and anti-inflammatory peptides, from salmon by-products, by electrodialysis with ultrafiltration membrane (EDUF), and to identify peptides responsible for these bioactivities. Two EDUF configurations (1 and 2) were used to concentrate anionic and cationic peptides, respectively. After EDUF separation, two fractions demonstrated interesting properties: the initial fraction of the EDUF configuration 1 and the final fraction of the EDUF configuration 2 both showed biological activities to (1) increase glucose uptake in L6 muscle cells in insulin condition at 1 ng/mL (by 12% and 21%, respectively), (2) decrease hepatic glucose production in hepatic cells at 1 ng/mL in basal (17% and 16%, respectively), and insulin (25% and 34%, respectively) conditions, and (3) decrease LPS-induced inflammation in macrophages at 1 g/mL (45% and 30%, respectively). More impressive, the initial fraction of the EDUF configuration 1 (45% reduction) showed the same effect as the phenformin at 10 µM (40%), a drug used to treat T2D. Thirteen peptides were identified, chemically synthesized, and tested in-vitro for these three bioactivities. Thus, four new bioactive peptides were identified: IPVE increased glucose uptake by muscle cells, IVDI and IEGTL decreased hepatic glucose production (HGP) of insulin, whereas VAPEEHPTL decreased HGP under both basal condition and in the presence of insulin. To the best of our knowledge, this is the first time that (1) bioactive peptide fractions generated after separation by EDUF were demonstrated to be bioactive on three different criteria; all involved in the T2D, and (2) potential sequences involved in the improvement of glucose uptake and/or in the regulation of HGP were identified from a salmon protein hydrolysate. |
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Glucoregulatory and anti-inflammatory activities of peptide fractions separated by electrodialysis with ultrafiltration membranes from salmon protein hydrolysate and identification of four novel glucoregulatory peptidesAnti-inflammatory activityBioactive peptidesElectrodialysis with filtration membraneGlucose uptakeHepatic glucose productionSalmon protein hydrolysateNatural bioactive peptides are suitable candidates for preventing the development of Type 2 diabetes (T2D), by reducing the various risk factors. The aim of this study was to concentrate glucoregulatory and anti-inflammatory peptides, from salmon by-products, by electrodialysis with ultrafiltration membrane (EDUF), and to identify peptides responsible for these bioactivities. Two EDUF configurations (1 and 2) were used to concentrate anionic and cationic peptides, respectively. After EDUF separation, two fractions demonstrated interesting properties: the initial fraction of the EDUF configuration 1 and the final fraction of the EDUF configuration 2 both showed biological activities to (1) increase glucose uptake in L6 muscle cells in insulin condition at 1 ng/mL (by 12% and 21%, respectively), (2) decrease hepatic glucose production in hepatic cells at 1 ng/mL in basal (17% and 16%, respectively), and insulin (25% and 34%, respectively) conditions, and (3) decrease LPS-induced inflammation in macrophages at 1 g/mL (45% and 30%, respectively). More impressive, the initial fraction of the EDUF configuration 1 (45% reduction) showed the same effect as the phenformin at 10 µM (40%), a drug used to treat T2D. Thirteen peptides were identified, chemically synthesized, and tested in-vitro for these three bioactivities. Thus, four new bioactive peptides were identified: IPVE increased glucose uptake by muscle cells, IVDI and IEGTL decreased hepatic glucose production (HGP) of insulin, whereas VAPEEHPTL decreased HGP under both basal condition and in the presence of insulin. To the best of our knowledge, this is the first time that (1) bioactive peptide fractions generated after separation by EDUF were demonstrated to be bioactive on three different criteria; all involved in the T2D, and (2) potential sequences involved in the improvement of glucose uptake and/or in the regulation of HGP were identified from a salmon protein hydrolysate.Canadian Institutes of Health ResearchDepartment of Food Sciences and Laboratory of Food Processing and Electromembrane Processes (LTAPEM) Université LavalInstitute of Nutrition and Functional Foods (INAF) University LavalLaboratory of Biotechnology School of Applied Sciences University of Campinas (UNICAMP)Institute of Biosciences State University (UNESP)Department of Medicine Faculty of Medicine Quebec Heart and Lung Institute Cardiology Group Université Laval, 2725 Chemin Ste-FoyDepartment of Process Engineering and Applied Science Dalhousie University, P.O. Box 15000Institute of Biosciences State University (UNESP)Canadian Institutes of Health Research: FH4-129922Université LavalUniversity LavalUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (UNESP)Dalhousie UniversityHenaux, LoïcPereira, Karina Danielle [UNESP]Thibodeau, JacinthePilon, GenevièveGill, TomMarette, AndréBazinet, Laurent2022-04-28T19:42:08Z2022-04-28T19:42:08Z2021-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/membranes11070528Membranes, v. 11, n. 7, 2021.2077-0375http://hdl.handle.net/11449/22205710.3390/membranes110705282-s2.0-85111273791Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMembranesinfo:eu-repo/semantics/openAccess2022-04-28T19:42:08Zoai:repositorio.unesp.br:11449/222057Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:32:34.720118Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Glucoregulatory and anti-inflammatory activities of peptide fractions separated by electrodialysis with ultrafiltration membranes from salmon protein hydrolysate and identification of four novel glucoregulatory peptides |
title |
Glucoregulatory and anti-inflammatory activities of peptide fractions separated by electrodialysis with ultrafiltration membranes from salmon protein hydrolysate and identification of four novel glucoregulatory peptides |
spellingShingle |
Glucoregulatory and anti-inflammatory activities of peptide fractions separated by electrodialysis with ultrafiltration membranes from salmon protein hydrolysate and identification of four novel glucoregulatory peptides Henaux, Loïc Anti-inflammatory activity Bioactive peptides Electrodialysis with filtration membrane Glucose uptake Hepatic glucose production Salmon protein hydrolysate |
title_short |
Glucoregulatory and anti-inflammatory activities of peptide fractions separated by electrodialysis with ultrafiltration membranes from salmon protein hydrolysate and identification of four novel glucoregulatory peptides |
title_full |
Glucoregulatory and anti-inflammatory activities of peptide fractions separated by electrodialysis with ultrafiltration membranes from salmon protein hydrolysate and identification of four novel glucoregulatory peptides |
title_fullStr |
Glucoregulatory and anti-inflammatory activities of peptide fractions separated by electrodialysis with ultrafiltration membranes from salmon protein hydrolysate and identification of four novel glucoregulatory peptides |
title_full_unstemmed |
Glucoregulatory and anti-inflammatory activities of peptide fractions separated by electrodialysis with ultrafiltration membranes from salmon protein hydrolysate and identification of four novel glucoregulatory peptides |
title_sort |
Glucoregulatory and anti-inflammatory activities of peptide fractions separated by electrodialysis with ultrafiltration membranes from salmon protein hydrolysate and identification of four novel glucoregulatory peptides |
author |
Henaux, Loïc |
author_facet |
Henaux, Loïc Pereira, Karina Danielle [UNESP] Thibodeau, Jacinthe Pilon, Geneviève Gill, Tom Marette, André Bazinet, Laurent |
author_role |
author |
author2 |
Pereira, Karina Danielle [UNESP] Thibodeau, Jacinthe Pilon, Geneviève Gill, Tom Marette, André Bazinet, Laurent |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Université Laval University Laval Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (UNESP) Dalhousie University |
dc.contributor.author.fl_str_mv |
Henaux, Loïc Pereira, Karina Danielle [UNESP] Thibodeau, Jacinthe Pilon, Geneviève Gill, Tom Marette, André Bazinet, Laurent |
dc.subject.por.fl_str_mv |
Anti-inflammatory activity Bioactive peptides Electrodialysis with filtration membrane Glucose uptake Hepatic glucose production Salmon protein hydrolysate |
topic |
Anti-inflammatory activity Bioactive peptides Electrodialysis with filtration membrane Glucose uptake Hepatic glucose production Salmon protein hydrolysate |
description |
Natural bioactive peptides are suitable candidates for preventing the development of Type 2 diabetes (T2D), by reducing the various risk factors. The aim of this study was to concentrate glucoregulatory and anti-inflammatory peptides, from salmon by-products, by electrodialysis with ultrafiltration membrane (EDUF), and to identify peptides responsible for these bioactivities. Two EDUF configurations (1 and 2) were used to concentrate anionic and cationic peptides, respectively. After EDUF separation, two fractions demonstrated interesting properties: the initial fraction of the EDUF configuration 1 and the final fraction of the EDUF configuration 2 both showed biological activities to (1) increase glucose uptake in L6 muscle cells in insulin condition at 1 ng/mL (by 12% and 21%, respectively), (2) decrease hepatic glucose production in hepatic cells at 1 ng/mL in basal (17% and 16%, respectively), and insulin (25% and 34%, respectively) conditions, and (3) decrease LPS-induced inflammation in macrophages at 1 g/mL (45% and 30%, respectively). More impressive, the initial fraction of the EDUF configuration 1 (45% reduction) showed the same effect as the phenformin at 10 µM (40%), a drug used to treat T2D. Thirteen peptides were identified, chemically synthesized, and tested in-vitro for these three bioactivities. Thus, four new bioactive peptides were identified: IPVE increased glucose uptake by muscle cells, IVDI and IEGTL decreased hepatic glucose production (HGP) of insulin, whereas VAPEEHPTL decreased HGP under both basal condition and in the presence of insulin. To the best of our knowledge, this is the first time that (1) bioactive peptide fractions generated after separation by EDUF were demonstrated to be bioactive on three different criteria; all involved in the T2D, and (2) potential sequences involved in the improvement of glucose uptake and/or in the regulation of HGP were identified from a salmon protein hydrolysate. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07-01 2022-04-28T19:42:08Z 2022-04-28T19:42:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/membranes11070528 Membranes, v. 11, n. 7, 2021. 2077-0375 http://hdl.handle.net/11449/222057 10.3390/membranes11070528 2-s2.0-85111273791 |
url |
http://dx.doi.org/10.3390/membranes11070528 http://hdl.handle.net/11449/222057 |
identifier_str_mv |
Membranes, v. 11, n. 7, 2021. 2077-0375 10.3390/membranes11070528 2-s2.0-85111273791 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Membranes |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128944477569024 |