Separation of bioactive peptides by electrodialysis with ultrafiltration membranes: membrane characteristics, ex-situ and in-situ digestion and their impact on peptide migration

Detalhes bibliográficos
Autor(a) principal: Manenda, Mahder Seifu
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/10410
Resumo: Dissertation presented to Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa for obtaining the master degree in Membrane Engineering
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spelling Separation of bioactive peptides by electrodialysis with ultrafiltration membranes: membrane characteristics, ex-situ and in-situ digestion and their impact on peptide migrationHydrolysisBioactive peptidesPeptide migrationElectrodialysisUltrafiltrationNutraceuticalsDissertation presented to Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa for obtaining the master degree in Membrane EngineeringTrypsic hydrolysis of whey protein isolate was performed simultaneously with (in-situ) and before (ex-situ) fractionation by electrodialysis with ultrafiltration membrane (EDUF) to obtain bioactive peptides. Two ultrafiltration membrane (UFM) materials, PES and PVDF, were used for a 120 minute EDUF fractionation of the hydrolysate. The two membranes showed similar zeta potential measurements at the pH of operation, 7.8, but had significantly different conductivity which decreased significantly after use in EDUF. Peptide migration to anionic (A-RC) and cationic (C+RC) peptide recovery compartment was strongly dependent on the electrical conductivity of the UFMs than their types or the digestion strategy used. For UFMs with close values of conductivity, peptide migration to the A-RC was observed to be higher with in-situ digestion while peptide migration to the C+RC was higher in an ex-situ digestion. When the two membrane types, PES and PVDF, have closer values of conductivities, PVDF was observed to exhibit more migration than PES. Peptide migration to the C+RC varied from 16.56 ± 5.36 μg/mL to 103.10 ± 2.76 μg/mL for PVDF membrane with significantly different conductivities: 2.73 ± 0.32 mS/cm and 5.47 ± 0.56 mS/cm, respectively. Peptide migration to the A-RC varied from 4.41 ± 0.86 μg/mL to 49.65 ± 6.13 μg/ml for PVDF membrane with significantly different conductivities: 3.163 ± 0.12 mS/cm and 5.23 ± 0.04 mS/cm, respectively. HPLC-MS studies showed 23 major peaks that were generated on whey protein isolate digestion by trypsin. 9 of these peaks migrated to the A-RC while 3 migrated to the C+RC. Among the anionic peptides 3 peptides are known to have hypocholesterolemic effect, 1 is antibacterial and 1 is antihypertensive. Among the cationic peptides 1 is antihypertensive. EDUF appears to be a powerful new technique that can fractionate bioactive peptides in terms of mass and charge.The EM3E Master is an Education Programme supported by the European Commission, the European Membrane Society (EMS), the European Membrane House (EMH), and a large international network of industrial companies, research centres and universitiesFaculdade de Ciências e TecnologiaBazinet, LaurentCoelhoso, IsabelCrespo, JoãoRUNManenda, Mahder Seifu2013-09-20T10:57:00Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/10410enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:44:10Zoai:run.unl.pt:10362/10410Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:19:27.579844Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Separation of bioactive peptides by electrodialysis with ultrafiltration membranes: membrane characteristics, ex-situ and in-situ digestion and their impact on peptide migration
title Separation of bioactive peptides by electrodialysis with ultrafiltration membranes: membrane characteristics, ex-situ and in-situ digestion and their impact on peptide migration
spellingShingle Separation of bioactive peptides by electrodialysis with ultrafiltration membranes: membrane characteristics, ex-situ and in-situ digestion and their impact on peptide migration
Manenda, Mahder Seifu
Hydrolysis
Bioactive peptides
Peptide migration
Electrodialysis
Ultrafiltration
Nutraceuticals
title_short Separation of bioactive peptides by electrodialysis with ultrafiltration membranes: membrane characteristics, ex-situ and in-situ digestion and their impact on peptide migration
title_full Separation of bioactive peptides by electrodialysis with ultrafiltration membranes: membrane characteristics, ex-situ and in-situ digestion and their impact on peptide migration
title_fullStr Separation of bioactive peptides by electrodialysis with ultrafiltration membranes: membrane characteristics, ex-situ and in-situ digestion and their impact on peptide migration
title_full_unstemmed Separation of bioactive peptides by electrodialysis with ultrafiltration membranes: membrane characteristics, ex-situ and in-situ digestion and their impact on peptide migration
title_sort Separation of bioactive peptides by electrodialysis with ultrafiltration membranes: membrane characteristics, ex-situ and in-situ digestion and their impact on peptide migration
author Manenda, Mahder Seifu
author_facet Manenda, Mahder Seifu
author_role author
dc.contributor.none.fl_str_mv Bazinet, Laurent
Coelhoso, Isabel
Crespo, João
RUN
dc.contributor.author.fl_str_mv Manenda, Mahder Seifu
dc.subject.por.fl_str_mv Hydrolysis
Bioactive peptides
Peptide migration
Electrodialysis
Ultrafiltration
Nutraceuticals
topic Hydrolysis
Bioactive peptides
Peptide migration
Electrodialysis
Ultrafiltration
Nutraceuticals
description Dissertation presented to Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa for obtaining the master degree in Membrane Engineering
publishDate 2013
dc.date.none.fl_str_mv 2013-09-20T10:57:00Z
2013
2013-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/10410
url http://hdl.handle.net/10362/10410
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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