The effect of halogen bulb and light-emitting diode light curing units on temperature increase and fibroblast viability [version 1; peer review: 1 approved, 1 approved with reservations]
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.12688/F1000RESEARCH.25456.1 http://hdl.handle.net/11449/229737 |
Resumo: | produced by halogen bulb (HAL) and light-emitting diode (LED) light curing units (LCUs) by irradiating dentin discs (0.5 mm and 1 mm thickness), and to evaluate their cytotoxic effects on fibroblast culture in the presence of dentin discs due to the increasing demand on resincomposite restorations and teeth bleaching for esthetic purposes. total of 20 bovine incisors were used to obtain dentin discs and divided into four experimental groups (n=10): HAL0.5: intensity of 470 mW/cm2 over a dentin disc of 0.5 mm; LED0.5: irradiation with LED Optilight Max (GNATUS-Ribeirão Preto, SP, Brazil) at an intensity of 1200 mW/cm2 over a dentin disc of 0.5 mm; HAL1: irradiation as in HAL0.5 but over a dentin disc of 1 mm; LED1: irradiation as in LED0.5 but over a dentin disc of 1 mm. The temperature increase was measured using a digital thermometer and the cytotoxicity was evaluated using an MTT assay with a mouse fibroblast cell line (L929). Parametric Data were analyzed by ANOVA and Tukey and non-parametric data were analyzed by Kruskal Wallis with Conover-Iman for non-parametric data (all with α=0.05). Results: A significant statistical difference was found between the groups HAL0.5 and HAL1 and both were different of LED0.5 and LED1 which presented higher temperature. All the experimental groups were different of the control group (without irradiation), and promoted reduction of cellular viability. Conclusions: HAL LCU promoted a lower temperature change in the to LED, regardless of the dentin thickness (0.5-1 mm). Both HAL and LED LCUs decreased fibroblast viability; however, LED promoted more significant cytotoxic effects. |
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The effect of halogen bulb and light-emitting diode light curing units on temperature increase and fibroblast viability [version 1; peer review: 1 approved, 1 approved with reservations]Halogen lightLight-emiting-diodemouse fibroblaststemperature increaseproduced by halogen bulb (HAL) and light-emitting diode (LED) light curing units (LCUs) by irradiating dentin discs (0.5 mm and 1 mm thickness), and to evaluate their cytotoxic effects on fibroblast culture in the presence of dentin discs due to the increasing demand on resincomposite restorations and teeth bleaching for esthetic purposes. total of 20 bovine incisors were used to obtain dentin discs and divided into four experimental groups (n=10): HAL0.5: intensity of 470 mW/cm2 over a dentin disc of 0.5 mm; LED0.5: irradiation with LED Optilight Max (GNATUS-Ribeirão Preto, SP, Brazil) at an intensity of 1200 mW/cm2 over a dentin disc of 0.5 mm; HAL1: irradiation as in HAL0.5 but over a dentin disc of 1 mm; LED1: irradiation as in LED0.5 but over a dentin disc of 1 mm. The temperature increase was measured using a digital thermometer and the cytotoxicity was evaluated using an MTT assay with a mouse fibroblast cell line (L929). Parametric Data were analyzed by ANOVA and Tukey and non-parametric data were analyzed by Kruskal Wallis with Conover-Iman for non-parametric data (all with α=0.05). Results: A significant statistical difference was found between the groups HAL0.5 and HAL1 and both were different of LED0.5 and LED1 which presented higher temperature. All the experimental groups were different of the control group (without irradiation), and promoted reduction of cellular viability. Conclusions: HAL LCU promoted a lower temperature change in the to LED, regardless of the dentin thickness (0.5-1 mm). Both HAL and LED LCUs decreased fibroblast viability; however, LED promoted more significant cytotoxic effects.Department of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and Technology, 12245000Taubaté Institute of Higher Education (ITES), Taubaté,12090000Department of Restorative Dentistry São Paulo State University (Unesp) Institute of Science and Technology São José dos Campos, 12245000Department of Enviormental São Paulo State University (Unesp) Institute of Science and Technology, 12247016Department of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and Technology, 12245000Department of Restorative Dentistry São Paulo State University (Unesp) Institute of Science and Technology São José dos Campos, 12245000Department of Enviormental São Paulo State University (Unesp) Institute of Science and Technology, 12247016Universidade Estadual Paulista (UNESP)Taubaté Institute of Higher Education (ITES)Trava, Georgia Memari [UNESP]Santos, Juliane Almeida [UNESP]Ramos, Lucas Paula [UNESP]Santos, Pamela Beatriz Rosário Estevam dos [UNESP]Hasna, Amjad Abu [UNESP]Yui, Karen Cristina [UNESP]Bressane, Adriano [UNESP]de Oliveira, Luciane Dias [UNESP]Spalding, Marianne [UNESP]2022-04-29T08:35:34Z2022-04-29T08:35:34Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-13http://dx.doi.org/10.12688/F1000RESEARCH.25456.1F1000Research, v. 9, p. 1-13.1759-796X2046-1402http://hdl.handle.net/11449/22973710.12688/F1000RESEARCH.25456.12-s2.0-85117351042Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengF1000Researchinfo:eu-repo/semantics/openAccess2022-04-29T08:35:34Zoai:repositorio.unesp.br:11449/229737Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:03:50.517682Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The effect of halogen bulb and light-emitting diode light curing units on temperature increase and fibroblast viability [version 1; peer review: 1 approved, 1 approved with reservations] |
title |
The effect of halogen bulb and light-emitting diode light curing units on temperature increase and fibroblast viability [version 1; peer review: 1 approved, 1 approved with reservations] |
spellingShingle |
The effect of halogen bulb and light-emitting diode light curing units on temperature increase and fibroblast viability [version 1; peer review: 1 approved, 1 approved with reservations] Trava, Georgia Memari [UNESP] Halogen light Light-emiting-diode mouse fibroblasts temperature increase |
title_short |
The effect of halogen bulb and light-emitting diode light curing units on temperature increase and fibroblast viability [version 1; peer review: 1 approved, 1 approved with reservations] |
title_full |
The effect of halogen bulb and light-emitting diode light curing units on temperature increase and fibroblast viability [version 1; peer review: 1 approved, 1 approved with reservations] |
title_fullStr |
The effect of halogen bulb and light-emitting diode light curing units on temperature increase and fibroblast viability [version 1; peer review: 1 approved, 1 approved with reservations] |
title_full_unstemmed |
The effect of halogen bulb and light-emitting diode light curing units on temperature increase and fibroblast viability [version 1; peer review: 1 approved, 1 approved with reservations] |
title_sort |
The effect of halogen bulb and light-emitting diode light curing units on temperature increase and fibroblast viability [version 1; peer review: 1 approved, 1 approved with reservations] |
author |
Trava, Georgia Memari [UNESP] |
author_facet |
Trava, Georgia Memari [UNESP] Santos, Juliane Almeida [UNESP] Ramos, Lucas Paula [UNESP] Santos, Pamela Beatriz Rosário Estevam dos [UNESP] Hasna, Amjad Abu [UNESP] Yui, Karen Cristina [UNESP] Bressane, Adriano [UNESP] de Oliveira, Luciane Dias [UNESP] Spalding, Marianne [UNESP] |
author_role |
author |
author2 |
Santos, Juliane Almeida [UNESP] Ramos, Lucas Paula [UNESP] Santos, Pamela Beatriz Rosário Estevam dos [UNESP] Hasna, Amjad Abu [UNESP] Yui, Karen Cristina [UNESP] Bressane, Adriano [UNESP] de Oliveira, Luciane Dias [UNESP] Spalding, Marianne [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Taubaté Institute of Higher Education (ITES) |
dc.contributor.author.fl_str_mv |
Trava, Georgia Memari [UNESP] Santos, Juliane Almeida [UNESP] Ramos, Lucas Paula [UNESP] Santos, Pamela Beatriz Rosário Estevam dos [UNESP] Hasna, Amjad Abu [UNESP] Yui, Karen Cristina [UNESP] Bressane, Adriano [UNESP] de Oliveira, Luciane Dias [UNESP] Spalding, Marianne [UNESP] |
dc.subject.por.fl_str_mv |
Halogen light Light-emiting-diode mouse fibroblasts temperature increase |
topic |
Halogen light Light-emiting-diode mouse fibroblasts temperature increase |
description |
produced by halogen bulb (HAL) and light-emitting diode (LED) light curing units (LCUs) by irradiating dentin discs (0.5 mm and 1 mm thickness), and to evaluate their cytotoxic effects on fibroblast culture in the presence of dentin discs due to the increasing demand on resincomposite restorations and teeth bleaching for esthetic purposes. total of 20 bovine incisors were used to obtain dentin discs and divided into four experimental groups (n=10): HAL0.5: intensity of 470 mW/cm2 over a dentin disc of 0.5 mm; LED0.5: irradiation with LED Optilight Max (GNATUS-Ribeirão Preto, SP, Brazil) at an intensity of 1200 mW/cm2 over a dentin disc of 0.5 mm; HAL1: irradiation as in HAL0.5 but over a dentin disc of 1 mm; LED1: irradiation as in LED0.5 but over a dentin disc of 1 mm. The temperature increase was measured using a digital thermometer and the cytotoxicity was evaluated using an MTT assay with a mouse fibroblast cell line (L929). Parametric Data were analyzed by ANOVA and Tukey and non-parametric data were analyzed by Kruskal Wallis with Conover-Iman for non-parametric data (all with α=0.05). Results: A significant statistical difference was found between the groups HAL0.5 and HAL1 and both were different of LED0.5 and LED1 which presented higher temperature. All the experimental groups were different of the control group (without irradiation), and promoted reduction of cellular viability. Conclusions: HAL LCU promoted a lower temperature change in the to LED, regardless of the dentin thickness (0.5-1 mm). Both HAL and LED LCUs decreased fibroblast viability; however, LED promoted more significant cytotoxic effects. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2022-04-29T08:35:34Z 2022-04-29T08:35:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.12688/F1000RESEARCH.25456.1 F1000Research, v. 9, p. 1-13. 1759-796X 2046-1402 http://hdl.handle.net/11449/229737 10.12688/F1000RESEARCH.25456.1 2-s2.0-85117351042 |
url |
http://dx.doi.org/10.12688/F1000RESEARCH.25456.1 http://hdl.handle.net/11449/229737 |
identifier_str_mv |
F1000Research, v. 9, p. 1-13. 1759-796X 2046-1402 10.12688/F1000RESEARCH.25456.1 2-s2.0-85117351042 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
F1000Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-13 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129579094638592 |