In Vitro Apoptotic Cell Death Assessment

Detalhes bibliográficos
Autor(a) principal: Pereira, Lilian Cristina [UNESP]
Data de Publicação: 2021
Outros Autores: de Souza, Alecsandra Oliveira, Miranda, Raul Ghiraldelli, Dorta, Daniel Junqueira [UNESP]
Tipo de documento: Capítulo de livro
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/978-1-0716-1091-6_17
http://hdl.handle.net/11449/207166
Resumo: Chemical compounds induce cytotoxicity by various mechanisms, including interference in membrane integrity, metabolism, cellular component degradation or release, and cell division. Between the classic death pathways, namely, autophagy, apoptosis, and necrosis, apoptosis have been in the focus for the last several years as an important pathway for the toxicity of different types of xenobiotics. Because of that, having the tools to evaluate it is key for understanding and explaining the toxicodynamics of different classes of substances. Here, we describe a wide array of classic assays that can be easily implemented to evaluate apoptosis induction.
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spelling In Vitro Apoptotic Cell Death AssessmentAnnexin-VApoptosisCell deathNuclear fragmentationPhosphatidilserine exposureChemical compounds induce cytotoxicity by various mechanisms, including interference in membrane integrity, metabolism, cellular component degradation or release, and cell division. Between the classic death pathways, namely, autophagy, apoptosis, and necrosis, apoptosis have been in the focus for the last several years as an important pathway for the toxicity of different types of xenobiotics. Because of that, having the tools to evaluate it is key for understanding and explaining the toxicodynamics of different classes of substances. Here, we describe a wide array of classic assays that can be easily implemented to evaluate apoptosis induction.Department of Clinical Toxicological and Bromatological Analysis Faculty of Pharmaceutical Sciences of Ribeirão Preto University of São PauloDepartment of Bioprocesses and Biotechnology Faculty of Agronomic Sciences of Botucatu São Paulo State UniversityCenter for Evaluation of Environmental Impact on Human Health (TOXICAM) BotucatuFederal Institute of Science and Technology Education of Rondônia—Campus Porto Velho CalamaFFCLRP-USP Department of Chemistry Faculty of Philosophy Sciences and Letters of Ribeirão Preto University of São PauloInstituto Nacional de Tecnologias Alternativas de Detecção Avaliação Toxicológica e Remoção de Micropututantes e Radioativos (INCT-DATREM) Unesp Instituto de Química, Caixa Postal 355, CEP: 14800-900Department of Bioprocesses and Biotechnology Faculty of Agronomic Sciences of Botucatu São Paulo State UniversityInstituto Nacional de Tecnologias Alternativas de Detecção Avaliação Toxicológica e Remoção de Micropututantes e Radioativos (INCT-DATREM) Unesp Instituto de Química, Caixa Postal 355, CEP: 14800-900Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)BotucatuFederal Institute of Science and Technology Education of Rondônia—Campus Porto Velho CalamaPereira, Lilian Cristina [UNESP]de Souza, Alecsandra OliveiraMiranda, Raul GhiraldelliDorta, Daniel Junqueira [UNESP]2021-06-25T10:50:03Z2021-06-25T10:50:03Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bookPart243-261http://dx.doi.org/10.1007/978-1-0716-1091-6_17Methods in Molecular Biology, v. 2240, p. 243-261.1940-60291064-3745http://hdl.handle.net/11449/20716610.1007/978-1-0716-1091-6_172-s2.0-85099703647Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMethods in Molecular Biologyinfo:eu-repo/semantics/openAccess2021-10-23T16:22:43Zoai:repositorio.unesp.br:11449/207166Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:04:08.237086Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv In Vitro Apoptotic Cell Death Assessment
title In Vitro Apoptotic Cell Death Assessment
spellingShingle In Vitro Apoptotic Cell Death Assessment
Pereira, Lilian Cristina [UNESP]
Annexin-V
Apoptosis
Cell death
Nuclear fragmentation
Phosphatidilserine exposure
title_short In Vitro Apoptotic Cell Death Assessment
title_full In Vitro Apoptotic Cell Death Assessment
title_fullStr In Vitro Apoptotic Cell Death Assessment
title_full_unstemmed In Vitro Apoptotic Cell Death Assessment
title_sort In Vitro Apoptotic Cell Death Assessment
author Pereira, Lilian Cristina [UNESP]
author_facet Pereira, Lilian Cristina [UNESP]
de Souza, Alecsandra Oliveira
Miranda, Raul Ghiraldelli
Dorta, Daniel Junqueira [UNESP]
author_role author
author2 de Souza, Alecsandra Oliveira
Miranda, Raul Ghiraldelli
Dorta, Daniel Junqueira [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
Botucatu
Federal Institute of Science and Technology Education of Rondônia—Campus Porto Velho Calama
dc.contributor.author.fl_str_mv Pereira, Lilian Cristina [UNESP]
de Souza, Alecsandra Oliveira
Miranda, Raul Ghiraldelli
Dorta, Daniel Junqueira [UNESP]
dc.subject.por.fl_str_mv Annexin-V
Apoptosis
Cell death
Nuclear fragmentation
Phosphatidilserine exposure
topic Annexin-V
Apoptosis
Cell death
Nuclear fragmentation
Phosphatidilserine exposure
description Chemical compounds induce cytotoxicity by various mechanisms, including interference in membrane integrity, metabolism, cellular component degradation or release, and cell division. Between the classic death pathways, namely, autophagy, apoptosis, and necrosis, apoptosis have been in the focus for the last several years as an important pathway for the toxicity of different types of xenobiotics. Because of that, having the tools to evaluate it is key for understanding and explaining the toxicodynamics of different classes of substances. Here, we describe a wide array of classic assays that can be easily implemented to evaluate apoptosis induction.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:50:03Z
2021-06-25T10:50:03Z
2021-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/bookPart
format bookPart
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/978-1-0716-1091-6_17
Methods in Molecular Biology, v. 2240, p. 243-261.
1940-6029
1064-3745
http://hdl.handle.net/11449/207166
10.1007/978-1-0716-1091-6_17
2-s2.0-85099703647
url http://dx.doi.org/10.1007/978-1-0716-1091-6_17
http://hdl.handle.net/11449/207166
identifier_str_mv Methods in Molecular Biology, v. 2240, p. 243-261.
1940-6029
1064-3745
10.1007/978-1-0716-1091-6_17
2-s2.0-85099703647
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Methods in Molecular Biology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 243-261
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129487170174976

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