Modulatory effects of guarana (Paullinia cupana) on adipogenesis
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/nu9060635 http://hdl.handle.net/11449/174781 |
Resumo: | Guarana (Paullinia cupana) is a plant originated in Brazil that presents a beneficial effect on body weight control and metabolic alterations. The aim of this study was to evaluate the effects of guarana on genes and miRNAs related to adipogenesis in 3T3L1 cells. The anti-adipogenic effect of guarana was evaluated by Oil Red-O staining. Gene and miRNA expression levels were determined by real time PCR. The Cebpα and β-catenin nuclear translocation were evaluated using immunocytochemistry. Our data indicated that the triglyceride-reducing effect of guarana was dose-dependent from 100 to 300 µg/mL (-12%, -20%, -24% and -40%, respectively, p > 0.0001). An up-regulation of the anti-adipogenic genes Wnt10b, Wnt3a, Wnt1, Gata3 and Dlk1 and a down-regulation of pro-adipogenic genes Cebpα, PparΓ and Creb1 were also observed. Furthermore, guarana repressed mmu-miR-27b-3p, mmu-miR-34b-5p and mmu-miR-760-5p, that contributed for up-regulation of their molecular targets Wnt3a, Wnt1 and Wnt10b. Additionally, cells treated with guarana presented an increase on β-catenin nuclear translocation (p > 0.0018). In summary, our data indicate that guarana has an anti-adipogenic potential due to its ability to modulate miRNAs and genes related to this process. Together our data demonstrate the important role of guarana as a putative therapeutic agent. |
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Modulatory effects of guarana (Paullinia cupana) on adipogenesis3t3L1AdipogenesisGuarana (paullinia cupana)MiRNAObesityWnt pathwayGuarana (Paullinia cupana) is a plant originated in Brazil that presents a beneficial effect on body weight control and metabolic alterations. The aim of this study was to evaluate the effects of guarana on genes and miRNAs related to adipogenesis in 3T3L1 cells. The anti-adipogenic effect of guarana was evaluated by Oil Red-O staining. Gene and miRNA expression levels were determined by real time PCR. The Cebpα and β-catenin nuclear translocation were evaluated using immunocytochemistry. Our data indicated that the triglyceride-reducing effect of guarana was dose-dependent from 100 to 300 µg/mL (-12%, -20%, -24% and -40%, respectively, p > 0.0001). An up-regulation of the anti-adipogenic genes Wnt10b, Wnt3a, Wnt1, Gata3 and Dlk1 and a down-regulation of pro-adipogenic genes Cebpα, PparΓ and Creb1 were also observed. Furthermore, guarana repressed mmu-miR-27b-3p, mmu-miR-34b-5p and mmu-miR-760-5p, that contributed for up-regulation of their molecular targets Wnt3a, Wnt1 and Wnt10b. Additionally, cells treated with guarana presented an increase on β-catenin nuclear translocation (p > 0.0018). In summary, our data indicate that guarana has an anti-adipogenic potential due to its ability to modulate miRNAs and genes related to this process. Together our data demonstrate the important role of guarana as a putative therapeutic agent.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Laboratory of Immunopharmacology and Molecular Biology Clinical Pharmacology and Gastroenterology Unit Sao Francisco University Medical SchoolLaboratory of Bioprospection and Natural products (LBPN) UNESP-São Paulo State University/Coastal Campus of Sao Vicente Pça Infante Dom Henrique S/NLaboratory of Bioprospection and Natural products (LBPN) UNESP-São Paulo State University/Coastal Campus of Sao Vicente Pça Infante Dom Henrique S/NSao Francisco University Medical SchoolUniversidade Estadual Paulista (Unesp)Da Silva Lima, NatáliaDe Paula Numata, EricaDe Souza Mesquita, Leonardo Mendes [UNESP]Dias, Pollyana Hammoud [UNESP]Vilegas, Wagner [UNESP]Gambero, AlessandraRibeiro, Marcelo Lima2018-12-11T17:12:49Z2018-12-11T17:12:49Z2017-06-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.3390/nu9060635Nutrients, v. 9, n. 6, 2017.2072-6643http://hdl.handle.net/11449/17478110.3390/nu90606352-s2.0-850210527422-s2.0-85021052742.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNutrients1,557info:eu-repo/semantics/openAccess2023-12-17T06:17:50Zoai:repositorio.unesp.br:11449/174781Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-17T06:17:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Modulatory effects of guarana (Paullinia cupana) on adipogenesis |
title |
Modulatory effects of guarana (Paullinia cupana) on adipogenesis |
spellingShingle |
Modulatory effects of guarana (Paullinia cupana) on adipogenesis Da Silva Lima, Natália 3t3L1 Adipogenesis Guarana (paullinia cupana) MiRNA Obesity Wnt pathway |
title_short |
Modulatory effects of guarana (Paullinia cupana) on adipogenesis |
title_full |
Modulatory effects of guarana (Paullinia cupana) on adipogenesis |
title_fullStr |
Modulatory effects of guarana (Paullinia cupana) on adipogenesis |
title_full_unstemmed |
Modulatory effects of guarana (Paullinia cupana) on adipogenesis |
title_sort |
Modulatory effects of guarana (Paullinia cupana) on adipogenesis |
author |
Da Silva Lima, Natália |
author_facet |
Da Silva Lima, Natália De Paula Numata, Erica De Souza Mesquita, Leonardo Mendes [UNESP] Dias, Pollyana Hammoud [UNESP] Vilegas, Wagner [UNESP] Gambero, Alessandra Ribeiro, Marcelo Lima |
author_role |
author |
author2 |
De Paula Numata, Erica De Souza Mesquita, Leonardo Mendes [UNESP] Dias, Pollyana Hammoud [UNESP] Vilegas, Wagner [UNESP] Gambero, Alessandra Ribeiro, Marcelo Lima |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Sao Francisco University Medical School Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Da Silva Lima, Natália De Paula Numata, Erica De Souza Mesquita, Leonardo Mendes [UNESP] Dias, Pollyana Hammoud [UNESP] Vilegas, Wagner [UNESP] Gambero, Alessandra Ribeiro, Marcelo Lima |
dc.subject.por.fl_str_mv |
3t3L1 Adipogenesis Guarana (paullinia cupana) MiRNA Obesity Wnt pathway |
topic |
3t3L1 Adipogenesis Guarana (paullinia cupana) MiRNA Obesity Wnt pathway |
description |
Guarana (Paullinia cupana) is a plant originated in Brazil that presents a beneficial effect on body weight control and metabolic alterations. The aim of this study was to evaluate the effects of guarana on genes and miRNAs related to adipogenesis in 3T3L1 cells. The anti-adipogenic effect of guarana was evaluated by Oil Red-O staining. Gene and miRNA expression levels were determined by real time PCR. The Cebpα and β-catenin nuclear translocation were evaluated using immunocytochemistry. Our data indicated that the triglyceride-reducing effect of guarana was dose-dependent from 100 to 300 µg/mL (-12%, -20%, -24% and -40%, respectively, p > 0.0001). An up-regulation of the anti-adipogenic genes Wnt10b, Wnt3a, Wnt1, Gata3 and Dlk1 and a down-regulation of pro-adipogenic genes Cebpα, PparΓ and Creb1 were also observed. Furthermore, guarana repressed mmu-miR-27b-3p, mmu-miR-34b-5p and mmu-miR-760-5p, that contributed for up-regulation of their molecular targets Wnt3a, Wnt1 and Wnt10b. Additionally, cells treated with guarana presented an increase on β-catenin nuclear translocation (p > 0.0018). In summary, our data indicate that guarana has an anti-adipogenic potential due to its ability to modulate miRNAs and genes related to this process. Together our data demonstrate the important role of guarana as a putative therapeutic agent. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06-20 2018-12-11T17:12:49Z 2018-12-11T17:12:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/nu9060635 Nutrients, v. 9, n. 6, 2017. 2072-6643 http://hdl.handle.net/11449/174781 10.3390/nu9060635 2-s2.0-85021052742 2-s2.0-85021052742.pdf |
url |
http://dx.doi.org/10.3390/nu9060635 http://hdl.handle.net/11449/174781 |
identifier_str_mv |
Nutrients, v. 9, n. 6, 2017. 2072-6643 10.3390/nu9060635 2-s2.0-85021052742 2-s2.0-85021052742.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Nutrients 1,557 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1803046986480353280 |