Antioxidant and antiglycation activities and inhibitory action of Passiflora cincinnata on collagenase, elastase and tyrosinase: in vitro and in silico study
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bcab.2022.102464 http://hdl.handle.net/11449/242198 |
Resumo: | Passiflora species are widely used in folk medicine due to their sedative and anxiolytic activities; however, scientific studies also showed different biological activities (antioxidant, anti-inflammatory, antidiabetic and antimicrobial). The present study aimed to characterize the phytochemical profile of P. cincinnata hydroethanolic extract, evaluate its antioxidant and antiglycation activities and evaluate in silico molecular docking of its main flavonoids. Phytochemical characterization was performed by quantification of total polyphenols and flavonoids content, and High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) analysis. Antioxidant activity was determined by 2,2-Diphenyl-1-picrylhydrazyl (DPPH), Ferric reducing antioxidant power (FRAP), Nitric oxide (NO) and Thiobarbituric acid reactive species (TBARS) tests. Antiglycation activity was determined by Relative Electrophoretic Mobility (REM) and free amino groups assays. P. cincinnata presented total polyphenols (62 mg gallic acid equivalent (GAE) g−1 dry weight (DW)) and flavonoids (57 mg rutin equivalent (RE) g−1 DW), besides HPLC-MS showed the presence of different flavonoids glucoside, mainly vitexin and orientin. The extract presented antioxidant activity by DPPH (76.60%), FRAP (276.70 μM trolox equivalent (TE) g−1 DW), TBARS (80.30% inhibition) and NO (10.10 μM mL−1 nitrite formed). REM demonstrated that extract presents antiglycation activity similar to Bovine serum albumin treated with Aminoguanidine; additionally, it showed 22% free amino groups. In silico assay demonstrated that orientin exhibited the highest interaction with DNA and the best docking score with elastase and collagenase. Therefore, the present study showed the protective properties of P. cincinnata hydroethanolic extract against oxidative stress and glycation. It also showed the presence of polyphenols and flavonoids, and their interaction with DNA and enzymes. |
id |
UNSP_45c4484e9e23da9bb87b00b68e047c95 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/242198 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Antioxidant and antiglycation activities and inhibitory action of Passiflora cincinnata on collagenase, elastase and tyrosinase: in vitro and in silico studyFree radicalMolecular dockingPassion fruitProtein glycationWild fruitPassiflora species are widely used in folk medicine due to their sedative and anxiolytic activities; however, scientific studies also showed different biological activities (antioxidant, anti-inflammatory, antidiabetic and antimicrobial). The present study aimed to characterize the phytochemical profile of P. cincinnata hydroethanolic extract, evaluate its antioxidant and antiglycation activities and evaluate in silico molecular docking of its main flavonoids. Phytochemical characterization was performed by quantification of total polyphenols and flavonoids content, and High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) analysis. Antioxidant activity was determined by 2,2-Diphenyl-1-picrylhydrazyl (DPPH), Ferric reducing antioxidant power (FRAP), Nitric oxide (NO) and Thiobarbituric acid reactive species (TBARS) tests. Antiglycation activity was determined by Relative Electrophoretic Mobility (REM) and free amino groups assays. P. cincinnata presented total polyphenols (62 mg gallic acid equivalent (GAE) g−1 dry weight (DW)) and flavonoids (57 mg rutin equivalent (RE) g−1 DW), besides HPLC-MS showed the presence of different flavonoids glucoside, mainly vitexin and orientin. The extract presented antioxidant activity by DPPH (76.60%), FRAP (276.70 μM trolox equivalent (TE) g−1 DW), TBARS (80.30% inhibition) and NO (10.10 μM mL−1 nitrite formed). REM demonstrated that extract presents antiglycation activity similar to Bovine serum albumin treated with Aminoguanidine; additionally, it showed 22% free amino groups. In silico assay demonstrated that orientin exhibited the highest interaction with DNA and the best docking score with elastase and collagenase. Therefore, the present study showed the protective properties of P. cincinnata hydroethanolic extract against oxidative stress and glycation. It also showed the presence of polyphenols and flavonoids, and their interaction with DNA and enzymes.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)São Paulo State University (UNESP) Institute of Chemistry, São PauloSão Paulo State University (UNESP) Departament of Chemistry Faculty of Scienzces, São PauloFundação Educacional do Município de Assis (FEMA), São PauloLaboratory of Molecular Modeling Institute of Biotechnology Federal University of Uberlândia, Minas GeraisSão Paulo State University (UNESP) School of Sciences Humanities and Languages Department of Biotechnology Laboratory of Herbal Medicine and Natural Products, São PauloSão Paulo State University (UNESP) Institute of Chemistry, São PauloSão Paulo State University (UNESP) Departament of Chemistry Faculty of Scienzces, São PauloSão Paulo State University (UNESP) School of Sciences Humanities and Languages Department of Biotechnology Laboratory of Herbal Medicine and Natural Products, São PauloUniversidade Estadual Paulista (UNESP)Fundação Educacional do Município de Assis (FEMA)Universidade Federal de Uberlândia (UFU)da Costa Gomes, Amanda [UNESP]Figueiredo, Célia Cristina Malaguti [UNESP]Granero, Filipe Oliveira [UNESP]Junior, João Luiz Bronzel [UNESP]Ximenes, Valdecir Farias [UNESP]Silva, Luciana PereiraNicolau-Junior, Nilsonda Silva, Regildo Márcio Gonçalves [UNESP]2023-03-02T11:50:43Z2023-03-02T11:50:43Z2022-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.bcab.2022.102464Biocatalysis and Agricultural Biotechnology, v. 44.1878-8181http://hdl.handle.net/11449/24219810.1016/j.bcab.2022.1024642-s2.0-85136644221Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiocatalysis and Agricultural Biotechnologyinfo:eu-repo/semantics/openAccess2024-04-29T18:17:21Zoai:repositorio.unesp.br:11449/242198Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-29T18:17:21Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antioxidant and antiglycation activities and inhibitory action of Passiflora cincinnata on collagenase, elastase and tyrosinase: in vitro and in silico study |
title |
Antioxidant and antiglycation activities and inhibitory action of Passiflora cincinnata on collagenase, elastase and tyrosinase: in vitro and in silico study |
spellingShingle |
Antioxidant and antiglycation activities and inhibitory action of Passiflora cincinnata on collagenase, elastase and tyrosinase: in vitro and in silico study da Costa Gomes, Amanda [UNESP] Free radical Molecular docking Passion fruit Protein glycation Wild fruit |
title_short |
Antioxidant and antiglycation activities and inhibitory action of Passiflora cincinnata on collagenase, elastase and tyrosinase: in vitro and in silico study |
title_full |
Antioxidant and antiglycation activities and inhibitory action of Passiflora cincinnata on collagenase, elastase and tyrosinase: in vitro and in silico study |
title_fullStr |
Antioxidant and antiglycation activities and inhibitory action of Passiflora cincinnata on collagenase, elastase and tyrosinase: in vitro and in silico study |
title_full_unstemmed |
Antioxidant and antiglycation activities and inhibitory action of Passiflora cincinnata on collagenase, elastase and tyrosinase: in vitro and in silico study |
title_sort |
Antioxidant and antiglycation activities and inhibitory action of Passiflora cincinnata on collagenase, elastase and tyrosinase: in vitro and in silico study |
author |
da Costa Gomes, Amanda [UNESP] |
author_facet |
da Costa Gomes, Amanda [UNESP] Figueiredo, Célia Cristina Malaguti [UNESP] Granero, Filipe Oliveira [UNESP] Junior, João Luiz Bronzel [UNESP] Ximenes, Valdecir Farias [UNESP] Silva, Luciana Pereira Nicolau-Junior, Nilson da Silva, Regildo Márcio Gonçalves [UNESP] |
author_role |
author |
author2 |
Figueiredo, Célia Cristina Malaguti [UNESP] Granero, Filipe Oliveira [UNESP] Junior, João Luiz Bronzel [UNESP] Ximenes, Valdecir Farias [UNESP] Silva, Luciana Pereira Nicolau-Junior, Nilson da Silva, Regildo Márcio Gonçalves [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Fundação Educacional do Município de Assis (FEMA) Universidade Federal de Uberlândia (UFU) |
dc.contributor.author.fl_str_mv |
da Costa Gomes, Amanda [UNESP] Figueiredo, Célia Cristina Malaguti [UNESP] Granero, Filipe Oliveira [UNESP] Junior, João Luiz Bronzel [UNESP] Ximenes, Valdecir Farias [UNESP] Silva, Luciana Pereira Nicolau-Junior, Nilson da Silva, Regildo Márcio Gonçalves [UNESP] |
dc.subject.por.fl_str_mv |
Free radical Molecular docking Passion fruit Protein glycation Wild fruit |
topic |
Free radical Molecular docking Passion fruit Protein glycation Wild fruit |
description |
Passiflora species are widely used in folk medicine due to their sedative and anxiolytic activities; however, scientific studies also showed different biological activities (antioxidant, anti-inflammatory, antidiabetic and antimicrobial). The present study aimed to characterize the phytochemical profile of P. cincinnata hydroethanolic extract, evaluate its antioxidant and antiglycation activities and evaluate in silico molecular docking of its main flavonoids. Phytochemical characterization was performed by quantification of total polyphenols and flavonoids content, and High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) analysis. Antioxidant activity was determined by 2,2-Diphenyl-1-picrylhydrazyl (DPPH), Ferric reducing antioxidant power (FRAP), Nitric oxide (NO) and Thiobarbituric acid reactive species (TBARS) tests. Antiglycation activity was determined by Relative Electrophoretic Mobility (REM) and free amino groups assays. P. cincinnata presented total polyphenols (62 mg gallic acid equivalent (GAE) g−1 dry weight (DW)) and flavonoids (57 mg rutin equivalent (RE) g−1 DW), besides HPLC-MS showed the presence of different flavonoids glucoside, mainly vitexin and orientin. The extract presented antioxidant activity by DPPH (76.60%), FRAP (276.70 μM trolox equivalent (TE) g−1 DW), TBARS (80.30% inhibition) and NO (10.10 μM mL−1 nitrite formed). REM demonstrated that extract presents antiglycation activity similar to Bovine serum albumin treated with Aminoguanidine; additionally, it showed 22% free amino groups. In silico assay demonstrated that orientin exhibited the highest interaction with DNA and the best docking score with elastase and collagenase. Therefore, the present study showed the protective properties of P. cincinnata hydroethanolic extract against oxidative stress and glycation. It also showed the presence of polyphenols and flavonoids, and their interaction with DNA and enzymes. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-09-01 2023-03-02T11:50:43Z 2023-03-02T11:50:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bcab.2022.102464 Biocatalysis and Agricultural Biotechnology, v. 44. 1878-8181 http://hdl.handle.net/11449/242198 10.1016/j.bcab.2022.102464 2-s2.0-85136644221 |
url |
http://dx.doi.org/10.1016/j.bcab.2022.102464 http://hdl.handle.net/11449/242198 |
identifier_str_mv |
Biocatalysis and Agricultural Biotechnology, v. 44. 1878-8181 10.1016/j.bcab.2022.102464 2-s2.0-85136644221 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biocatalysis and Agricultural Biotechnology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965548436520960 |