Nanostructured screen-printed electrodes modified with self-assembled monolayers for determination of metronidazole in different matrices
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.5935/0103-5053.20140170 http://hdl.handle.net/11449/114542 |
Resumo: | This paper describes the development of a sensor for the determination of metronidazole using a simple method involving the creation of nanoporous structures on gold electrodes followed by modification with self-assembled monolayers (SAMs) of thiols. The initial formation of oxidized gold species at pH 7.0 was followed by potentiodynamic reduction in order to obtain a nanostructured surface that was then modified with a SAM of cysteine. The sensor was characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and electrochemical techniques. Quantification of metronidazole was performed using optimized square wave voltammetry. Under the best analytical conditions, the sensor operated at -0.36 V showed a linear range of 50-300 µmol L-1 and a limit of detection (LOD) of 2.6 µmol L-1. Evaluation of selectivity demonstrated that the electrode provided higher sensitivity towards nitroimidazoles. The sensor responses showed that it could be used for detection of metronidazole in different types of matrix. |
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Repositório Institucional da UNESP |
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2946 |
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Nanostructured screen-printed electrodes modified with self-assembled monolayers for determination of metronidazole in different matricesself-assembled monolayerscysteinemetronidazolenanostructured gold electrodeThis paper describes the development of a sensor for the determination of metronidazole using a simple method involving the creation of nanoporous structures on gold electrodes followed by modification with self-assembled monolayers (SAMs) of thiols. The initial formation of oxidized gold species at pH 7.0 was followed by potentiodynamic reduction in order to obtain a nanostructured surface that was then modified with a SAM of cysteine. The sensor was characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and electrochemical techniques. Quantification of metronidazole was performed using optimized square wave voltammetry. Under the best analytical conditions, the sensor operated at -0.36 V showed a linear range of 50-300 µmol L-1 and a limit of detection (LOD) of 2.6 µmol L-1. Evaluation of selectivity demonstrated that the electrode provided higher sensitivity towards nitroimidazoles. The sensor responses showed that it could be used for detection of metronidazole in different types of matrix.Neste trabalho é descrito o desenvolvimento de um sensor para detecção de metronidazol, usando um método simples para preparar estruturas nanoporosas de ouro em eletrodos impressos comerciais modificados com monocamadas autoarranjadas de tiol (SAMs). O processo envolve a formação inicial de espécies de ouro oxidadas a pH 7,0, e posteriormente a redução potenciodinâmica para a formação da superficie nanoestruturada, que a seguir foi modificada com cisteína para obtenção do sensor, o qual foi posteriormente caracterizado utilizando microscópio eletrônico de varredura (SEM), microscópio de forca atômica (AFM) e técnicas eletroquímicas. Para a quantificação de metronidazol foi otimizada a voltametria de onda quadrada (SWV). Nas melhores condições de análise o sensor mostrou em -0,36 V uma faixa linear entre 50 e 300 µmol L-1 e limite de detecção (LOD) = 2,6 µmol L-1. No estudo de seletividade, o eletrodo mostrou mais alta sensibilidade para os nitroimidazois. A resposta do sensor indica ser viável para a detecção deste fármaco em diferentes tipos de matrizes.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidad Nacional de Ingeniería Facultad de CienciasUniversidad de Lima Facultad de Ingeniería IndustrialUniversidade Estadual Paulista (UNESP) Instituto de Química Departamento de Química AnalíticaUniversidade Estadual Paulista (UNESP) Instituto de Química Departamento de Química AnalíticaSociedade Brasileira de QuímicaUniversidad Nacional de Ingeniería Facultad de CienciasUniversidad de Lima Facultad de Ingeniería IndustrialUniversidade Estadual Paulista (Unesp)Huayhuas-chipana, Bryan C. [UNESP]Gomero, Juan C. M.Sotomayor, Maria Del Pilar Taboada [UNESP]2015-02-02T12:39:37Z2015-02-02T12:39:37Z2014-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1737-1745application/pdfhttp://dx.doi.org/10.5935/0103-5053.20140170Journal of the Brazilian Chemical Society. Sociedade Brasileira de Química, v. 25, n. 9, p. 1737-1745, 2014.0103-5053http://hdl.handle.net/11449/11454210.5935/0103-5053.20140170S0103-50532014000900026S0103-50532014000900026.pdf2933194342093387SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of the Brazilian Chemical Society1.4440,357info:eu-repo/semantics/openAccess2023-11-18T06:16:02Zoai:repositorio.unesp.br:11449/114542Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:05:10.815090Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Nanostructured screen-printed electrodes modified with self-assembled monolayers for determination of metronidazole in different matrices |
title |
Nanostructured screen-printed electrodes modified with self-assembled monolayers for determination of metronidazole in different matrices |
spellingShingle |
Nanostructured screen-printed electrodes modified with self-assembled monolayers for determination of metronidazole in different matrices Huayhuas-chipana, Bryan C. [UNESP] self-assembled monolayers cysteine metronidazole nanostructured gold electrode |
title_short |
Nanostructured screen-printed electrodes modified with self-assembled monolayers for determination of metronidazole in different matrices |
title_full |
Nanostructured screen-printed electrodes modified with self-assembled monolayers for determination of metronidazole in different matrices |
title_fullStr |
Nanostructured screen-printed electrodes modified with self-assembled monolayers for determination of metronidazole in different matrices |
title_full_unstemmed |
Nanostructured screen-printed electrodes modified with self-assembled monolayers for determination of metronidazole in different matrices |
title_sort |
Nanostructured screen-printed electrodes modified with self-assembled monolayers for determination of metronidazole in different matrices |
author |
Huayhuas-chipana, Bryan C. [UNESP] |
author_facet |
Huayhuas-chipana, Bryan C. [UNESP] Gomero, Juan C. M. Sotomayor, Maria Del Pilar Taboada [UNESP] |
author_role |
author |
author2 |
Gomero, Juan C. M. Sotomayor, Maria Del Pilar Taboada [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidad Nacional de Ingeniería Facultad de Ciencias Universidad de Lima Facultad de Ingeniería Industrial Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Huayhuas-chipana, Bryan C. [UNESP] Gomero, Juan C. M. Sotomayor, Maria Del Pilar Taboada [UNESP] |
dc.subject.por.fl_str_mv |
self-assembled monolayers cysteine metronidazole nanostructured gold electrode |
topic |
self-assembled monolayers cysteine metronidazole nanostructured gold electrode |
description |
This paper describes the development of a sensor for the determination of metronidazole using a simple method involving the creation of nanoporous structures on gold electrodes followed by modification with self-assembled monolayers (SAMs) of thiols. The initial formation of oxidized gold species at pH 7.0 was followed by potentiodynamic reduction in order to obtain a nanostructured surface that was then modified with a SAM of cysteine. The sensor was characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), and electrochemical techniques. Quantification of metronidazole was performed using optimized square wave voltammetry. Under the best analytical conditions, the sensor operated at -0.36 V showed a linear range of 50-300 µmol L-1 and a limit of detection (LOD) of 2.6 µmol L-1. Evaluation of selectivity demonstrated that the electrode provided higher sensitivity towards nitroimidazoles. The sensor responses showed that it could be used for detection of metronidazole in different types of matrix. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09-01 2015-02-02T12:39:37Z 2015-02-02T12:39:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.5935/0103-5053.20140170 Journal of the Brazilian Chemical Society. Sociedade Brasileira de Química, v. 25, n. 9, p. 1737-1745, 2014. 0103-5053 http://hdl.handle.net/11449/114542 10.5935/0103-5053.20140170 S0103-50532014000900026 S0103-50532014000900026.pdf 2933194342093387 |
url |
http://dx.doi.org/10.5935/0103-5053.20140170 http://hdl.handle.net/11449/114542 |
identifier_str_mv |
Journal of the Brazilian Chemical Society. Sociedade Brasileira de Química, v. 25, n. 9, p. 1737-1745, 2014. 0103-5053 10.5935/0103-5053.20140170 S0103-50532014000900026 S0103-50532014000900026.pdf 2933194342093387 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of the Brazilian Chemical Society 1.444 0,357 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1737-1745 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128892330835968 |