Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00784-015-1671-5 http://hdl.handle.net/11449/159162 |
Resumo: | This study aims to synthesize and characterize biodegradable polymer-based matrices loaded with CaO nanoparticles for osteomyelitis treatment and bone tissue engineering. Poly(epsilon-caprolactone) (PCL) and PCL/gelatin (1:1, w/w) solutions containing CaO nanoparticles were electrospun into fibrous matrices. Scanning (SEM) and transmission (TEM) electron microscopy, Fourier transformed infrared (FTIR), energy dispersive X-ray spectroscopy (EDS), contact angle (CA), tensile testing, and antibacterial activity (agar diffusion assay) against Staphylococcus aureus were performed. Osteoprecursor cell (MC3T3-E1) response (i.e., viability and alkaline phosphatase expression/ALP) and infiltration into the matrices were evaluated. CaO nanoparticles were successfully incorporated into the fibers, with the median fiber diameter decreasing after CaO incorporation. The CA decreased with the addition of CaO, and the presence of gelatin made the matrix very hydrophilic (CA = 0A degrees). Increasing CaO concentrations progressively reduced the mechanical properties (p aecurrency sign 0.030). CaO-loaded matrices did not display consistent antibacterial activity. MC3T3-E1 cell viability demonstrated the highest levels for CaO-loaded matrices containing gelatin after 7 days in culture. An increased ALP expression was consistently seen for PCL/CaO matrices when compared to PCL and gelatin-containing counterparts. Despite inconsistent antibacterial activity, CaO nanoparticles can be effectively loaded into PCL or PCL/gelatin fibers without negatively affecting the overall performance of the matrices. More importantly, CaO incorporation enhanced cell viability as well as differentiation capacity, as demonstrated by an increased ALP expression. CaO-loaded electrospun matrices show potential for applications in bone tissue engineering. |
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Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineeringCaONanofibersOsteomyelitisElectrospinningBacteriaMC3T3-E1This study aims to synthesize and characterize biodegradable polymer-based matrices loaded with CaO nanoparticles for osteomyelitis treatment and bone tissue engineering. Poly(epsilon-caprolactone) (PCL) and PCL/gelatin (1:1, w/w) solutions containing CaO nanoparticles were electrospun into fibrous matrices. Scanning (SEM) and transmission (TEM) electron microscopy, Fourier transformed infrared (FTIR), energy dispersive X-ray spectroscopy (EDS), contact angle (CA), tensile testing, and antibacterial activity (agar diffusion assay) against Staphylococcus aureus were performed. Osteoprecursor cell (MC3T3-E1) response (i.e., viability and alkaline phosphatase expression/ALP) and infiltration into the matrices were evaluated. CaO nanoparticles were successfully incorporated into the fibers, with the median fiber diameter decreasing after CaO incorporation. The CA decreased with the addition of CaO, and the presence of gelatin made the matrix very hydrophilic (CA = 0A degrees). Increasing CaO concentrations progressively reduced the mechanical properties (p aecurrency sign 0.030). CaO-loaded matrices did not display consistent antibacterial activity. MC3T3-E1 cell viability demonstrated the highest levels for CaO-loaded matrices containing gelatin after 7 days in culture. An increased ALP expression was consistently seen for PCL/CaO matrices when compared to PCL and gelatin-containing counterparts. Despite inconsistent antibacterial activity, CaO nanoparticles can be effectively loaded into PCL or PCL/gelatin fibers without negatively affecting the overall performance of the matrices. More importantly, CaO incorporation enhanced cell viability as well as differentiation capacity, as demonstrated by an increased ALP expression. CaO-loaded electrospun matrices show potential for applications in bone tissue engineering.International Development Funds (IDF) Grant from Indiana University Purdue University (IUPUI/OVCR)IU School of DentistryNIH-NIDCRIndiana Clinical and Translational Sciences InstituteIndiana Univ, Sch Dent, Dept Biomed & Appl Sci, Div Dent Biomat, 1121 W Michigan St, Indianapolis, IN 46202 USAFed Univ Pelotas UFPel, Sch Dent, BR-96065560 Pelotas, RS, BrazilUniv Estadual Paulista, Grad Program Dent, Sao Jose dos Campos Dent Sch, BR-12245000 Sao Paulo, BrazilUniv Estadual Paulista, Grad Program Dent, Sao Jose dos Campos Dent Sch, BR-12245000 Sao Paulo, BrazilNIH-NIDCR: DE023552Indiana Clinical and Translational Sciences Institute: UL1 TR001108SpringerIndiana UnivUniversidade Federal de Pernambuco (UFPE)Universidade Estadual Paulista (Unesp)Munchow, Eliseu A.Pankajakshan, DivyaAlbuquerque, Maria T. P. [UNESP]Kamocki, KrzysztofPiva, EvandroGregory, Richard L.Bottino, Marco C.2018-11-26T15:31:38Z2018-11-26T15:31:38Z2016-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1921-1933application/pdfhttp://dx.doi.org/10.1007/s00784-015-1671-5Clinical Oral Investigations. Heidelberg: Springer Heidelberg, v. 20, n. 8, p. 1921-1933, 2016.1432-6981http://hdl.handle.net/11449/15916210.1007/s00784-015-1671-5WOS:000386501800008WOS000386501800008.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigations0,986info:eu-repo/semantics/openAccess2023-10-06T06:03:41Zoai:repositorio.unesp.br:11449/159162Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:09:13.848759Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering |
title |
Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering |
spellingShingle |
Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering Munchow, Eliseu A. CaO Nanofibers Osteomyelitis Electrospinning Bacteria MC3T3-E1 |
title_short |
Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering |
title_full |
Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering |
title_fullStr |
Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering |
title_full_unstemmed |
Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering |
title_sort |
Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering |
author |
Munchow, Eliseu A. |
author_facet |
Munchow, Eliseu A. Pankajakshan, Divya Albuquerque, Maria T. P. [UNESP] Kamocki, Krzysztof Piva, Evandro Gregory, Richard L. Bottino, Marco C. |
author_role |
author |
author2 |
Pankajakshan, Divya Albuquerque, Maria T. P. [UNESP] Kamocki, Krzysztof Piva, Evandro Gregory, Richard L. Bottino, Marco C. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Indiana Univ Universidade Federal de Pernambuco (UFPE) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Munchow, Eliseu A. Pankajakshan, Divya Albuquerque, Maria T. P. [UNESP] Kamocki, Krzysztof Piva, Evandro Gregory, Richard L. Bottino, Marco C. |
dc.subject.por.fl_str_mv |
CaO Nanofibers Osteomyelitis Electrospinning Bacteria MC3T3-E1 |
topic |
CaO Nanofibers Osteomyelitis Electrospinning Bacteria MC3T3-E1 |
description |
This study aims to synthesize and characterize biodegradable polymer-based matrices loaded with CaO nanoparticles for osteomyelitis treatment and bone tissue engineering. Poly(epsilon-caprolactone) (PCL) and PCL/gelatin (1:1, w/w) solutions containing CaO nanoparticles were electrospun into fibrous matrices. Scanning (SEM) and transmission (TEM) electron microscopy, Fourier transformed infrared (FTIR), energy dispersive X-ray spectroscopy (EDS), contact angle (CA), tensile testing, and antibacterial activity (agar diffusion assay) against Staphylococcus aureus were performed. Osteoprecursor cell (MC3T3-E1) response (i.e., viability and alkaline phosphatase expression/ALP) and infiltration into the matrices were evaluated. CaO nanoparticles were successfully incorporated into the fibers, with the median fiber diameter decreasing after CaO incorporation. The CA decreased with the addition of CaO, and the presence of gelatin made the matrix very hydrophilic (CA = 0A degrees). Increasing CaO concentrations progressively reduced the mechanical properties (p aecurrency sign 0.030). CaO-loaded matrices did not display consistent antibacterial activity. MC3T3-E1 cell viability demonstrated the highest levels for CaO-loaded matrices containing gelatin after 7 days in culture. An increased ALP expression was consistently seen for PCL/CaO matrices when compared to PCL and gelatin-containing counterparts. Despite inconsistent antibacterial activity, CaO nanoparticles can be effectively loaded into PCL or PCL/gelatin fibers without negatively affecting the overall performance of the matrices. More importantly, CaO incorporation enhanced cell viability as well as differentiation capacity, as demonstrated by an increased ALP expression. CaO-loaded electrospun matrices show potential for applications in bone tissue engineering. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11-01 2018-11-26T15:31:38Z 2018-11-26T15:31:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00784-015-1671-5 Clinical Oral Investigations. Heidelberg: Springer Heidelberg, v. 20, n. 8, p. 1921-1933, 2016. 1432-6981 http://hdl.handle.net/11449/159162 10.1007/s00784-015-1671-5 WOS:000386501800008 WOS000386501800008.pdf |
url |
http://dx.doi.org/10.1007/s00784-015-1671-5 http://hdl.handle.net/11449/159162 |
identifier_str_mv |
Clinical Oral Investigations. Heidelberg: Springer Heidelberg, v. 20, n. 8, p. 1921-1933, 2016. 1432-6981 10.1007/s00784-015-1671-5 WOS:000386501800008 WOS000386501800008.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Oral Investigations 0,986 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1921-1933 application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128323041099776 |