Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering

Detalhes bibliográficos
Autor(a) principal: Munchow, Eliseu A.
Data de Publicação: 2016
Outros Autores: Pankajakshan, Divya, Albuquerque, Maria T. P. [UNESP], Kamocki, Krzysztof, Piva, Evandro, Gregory, Richard L., Bottino, Marco C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00784-015-1671-5
http://hdl.handle.net/11449/159162
Resumo: This study aims to synthesize and characterize biodegradable polymer-based matrices loaded with CaO nanoparticles for osteomyelitis treatment and bone tissue engineering. Poly(epsilon-caprolactone) (PCL) and PCL/gelatin (1:1, w/w) solutions containing CaO nanoparticles were electrospun into fibrous matrices. Scanning (SEM) and transmission (TEM) electron microscopy, Fourier transformed infrared (FTIR), energy dispersive X-ray spectroscopy (EDS), contact angle (CA), tensile testing, and antibacterial activity (agar diffusion assay) against Staphylococcus aureus were performed. Osteoprecursor cell (MC3T3-E1) response (i.e., viability and alkaline phosphatase expression/ALP) and infiltration into the matrices were evaluated. CaO nanoparticles were successfully incorporated into the fibers, with the median fiber diameter decreasing after CaO incorporation. The CA decreased with the addition of CaO, and the presence of gelatin made the matrix very hydrophilic (CA = 0A degrees). Increasing CaO concentrations progressively reduced the mechanical properties (p aecurrency sign 0.030). CaO-loaded matrices did not display consistent antibacterial activity. MC3T3-E1 cell viability demonstrated the highest levels for CaO-loaded matrices containing gelatin after 7 days in culture. An increased ALP expression was consistently seen for PCL/CaO matrices when compared to PCL and gelatin-containing counterparts. Despite inconsistent antibacterial activity, CaO nanoparticles can be effectively loaded into PCL or PCL/gelatin fibers without negatively affecting the overall performance of the matrices. More importantly, CaO incorporation enhanced cell viability as well as differentiation capacity, as demonstrated by an increased ALP expression. CaO-loaded electrospun matrices show potential for applications in bone tissue engineering.
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spelling Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineeringCaONanofibersOsteomyelitisElectrospinningBacteriaMC3T3-E1This study aims to synthesize and characterize biodegradable polymer-based matrices loaded with CaO nanoparticles for osteomyelitis treatment and bone tissue engineering. Poly(epsilon-caprolactone) (PCL) and PCL/gelatin (1:1, w/w) solutions containing CaO nanoparticles were electrospun into fibrous matrices. Scanning (SEM) and transmission (TEM) electron microscopy, Fourier transformed infrared (FTIR), energy dispersive X-ray spectroscopy (EDS), contact angle (CA), tensile testing, and antibacterial activity (agar diffusion assay) against Staphylococcus aureus were performed. Osteoprecursor cell (MC3T3-E1) response (i.e., viability and alkaline phosphatase expression/ALP) and infiltration into the matrices were evaluated. CaO nanoparticles were successfully incorporated into the fibers, with the median fiber diameter decreasing after CaO incorporation. The CA decreased with the addition of CaO, and the presence of gelatin made the matrix very hydrophilic (CA = 0A degrees). Increasing CaO concentrations progressively reduced the mechanical properties (p aecurrency sign 0.030). CaO-loaded matrices did not display consistent antibacterial activity. MC3T3-E1 cell viability demonstrated the highest levels for CaO-loaded matrices containing gelatin after 7 days in culture. An increased ALP expression was consistently seen for PCL/CaO matrices when compared to PCL and gelatin-containing counterparts. Despite inconsistent antibacterial activity, CaO nanoparticles can be effectively loaded into PCL or PCL/gelatin fibers without negatively affecting the overall performance of the matrices. More importantly, CaO incorporation enhanced cell viability as well as differentiation capacity, as demonstrated by an increased ALP expression. CaO-loaded electrospun matrices show potential for applications in bone tissue engineering.International Development Funds (IDF) Grant from Indiana University Purdue University (IUPUI/OVCR)IU School of DentistryNIH-NIDCRIndiana Clinical and Translational Sciences InstituteIndiana Univ, Sch Dent, Dept Biomed & Appl Sci, Div Dent Biomat, 1121 W Michigan St, Indianapolis, IN 46202 USAFed Univ Pelotas UFPel, Sch Dent, BR-96065560 Pelotas, RS, BrazilUniv Estadual Paulista, Grad Program Dent, Sao Jose dos Campos Dent Sch, BR-12245000 Sao Paulo, BrazilUniv Estadual Paulista, Grad Program Dent, Sao Jose dos Campos Dent Sch, BR-12245000 Sao Paulo, BrazilNIH-NIDCR: DE023552Indiana Clinical and Translational Sciences Institute: UL1 TR001108SpringerIndiana UnivUniversidade Federal de Pernambuco (UFPE)Universidade Estadual Paulista (Unesp)Munchow, Eliseu A.Pankajakshan, DivyaAlbuquerque, Maria T. P. [UNESP]Kamocki, KrzysztofPiva, EvandroGregory, Richard L.Bottino, Marco C.2018-11-26T15:31:38Z2018-11-26T15:31:38Z2016-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1921-1933application/pdfhttp://dx.doi.org/10.1007/s00784-015-1671-5Clinical Oral Investigations. Heidelberg: Springer Heidelberg, v. 20, n. 8, p. 1921-1933, 2016.1432-6981http://hdl.handle.net/11449/15916210.1007/s00784-015-1671-5WOS:000386501800008WOS000386501800008.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigations0,986info:eu-repo/semantics/openAccess2023-10-06T06:03:41Zoai:repositorio.unesp.br:11449/159162Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:09:13.848759Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering
title Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering
spellingShingle Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering
Munchow, Eliseu A.
CaO
Nanofibers
Osteomyelitis
Electrospinning
Bacteria
MC3T3-E1
title_short Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering
title_full Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering
title_fullStr Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering
title_full_unstemmed Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering
title_sort Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering
author Munchow, Eliseu A.
author_facet Munchow, Eliseu A.
Pankajakshan, Divya
Albuquerque, Maria T. P. [UNESP]
Kamocki, Krzysztof
Piva, Evandro
Gregory, Richard L.
Bottino, Marco C.
author_role author
author2 Pankajakshan, Divya
Albuquerque, Maria T. P. [UNESP]
Kamocki, Krzysztof
Piva, Evandro
Gregory, Richard L.
Bottino, Marco C.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Indiana Univ
Universidade Federal de Pernambuco (UFPE)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Munchow, Eliseu A.
Pankajakshan, Divya
Albuquerque, Maria T. P. [UNESP]
Kamocki, Krzysztof
Piva, Evandro
Gregory, Richard L.
Bottino, Marco C.
dc.subject.por.fl_str_mv CaO
Nanofibers
Osteomyelitis
Electrospinning
Bacteria
MC3T3-E1
topic CaO
Nanofibers
Osteomyelitis
Electrospinning
Bacteria
MC3T3-E1
description This study aims to synthesize and characterize biodegradable polymer-based matrices loaded with CaO nanoparticles for osteomyelitis treatment and bone tissue engineering. Poly(epsilon-caprolactone) (PCL) and PCL/gelatin (1:1, w/w) solutions containing CaO nanoparticles were electrospun into fibrous matrices. Scanning (SEM) and transmission (TEM) electron microscopy, Fourier transformed infrared (FTIR), energy dispersive X-ray spectroscopy (EDS), contact angle (CA), tensile testing, and antibacterial activity (agar diffusion assay) against Staphylococcus aureus were performed. Osteoprecursor cell (MC3T3-E1) response (i.e., viability and alkaline phosphatase expression/ALP) and infiltration into the matrices were evaluated. CaO nanoparticles were successfully incorporated into the fibers, with the median fiber diameter decreasing after CaO incorporation. The CA decreased with the addition of CaO, and the presence of gelatin made the matrix very hydrophilic (CA = 0A degrees). Increasing CaO concentrations progressively reduced the mechanical properties (p aecurrency sign 0.030). CaO-loaded matrices did not display consistent antibacterial activity. MC3T3-E1 cell viability demonstrated the highest levels for CaO-loaded matrices containing gelatin after 7 days in culture. An increased ALP expression was consistently seen for PCL/CaO matrices when compared to PCL and gelatin-containing counterparts. Despite inconsistent antibacterial activity, CaO nanoparticles can be effectively loaded into PCL or PCL/gelatin fibers without negatively affecting the overall performance of the matrices. More importantly, CaO incorporation enhanced cell viability as well as differentiation capacity, as demonstrated by an increased ALP expression. CaO-loaded electrospun matrices show potential for applications in bone tissue engineering.
publishDate 2016
dc.date.none.fl_str_mv 2016-11-01
2018-11-26T15:31:38Z
2018-11-26T15:31:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00784-015-1671-5
Clinical Oral Investigations. Heidelberg: Springer Heidelberg, v. 20, n. 8, p. 1921-1933, 2016.
1432-6981
http://hdl.handle.net/11449/159162
10.1007/s00784-015-1671-5
WOS:000386501800008
WOS000386501800008.pdf
url http://dx.doi.org/10.1007/s00784-015-1671-5
http://hdl.handle.net/11449/159162
identifier_str_mv Clinical Oral Investigations. Heidelberg: Springer Heidelberg, v. 20, n. 8, p. 1921-1933, 2016.
1432-6981
10.1007/s00784-015-1671-5
WOS:000386501800008
WOS000386501800008.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Clinical Oral Investigations
0,986
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1921-1933
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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