Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.dental.2015.06.004 http://hdl.handle.net/11449/160864 |
Resumo: | Objectives. This study reports on the synthesis, materials characterization, antimicrobial capacity, and cytocompatibility of novel ZnO-loaded membranes for guided tissue/bone regeneration (GTR/GBR). Methods. Poly(epsilon-caprolactone) (PCL) and PCL/gelatin (PCL/GEL) were dissolved in hexafluoropropanol and loaded with ZnO at distinct concentrations: 0 (control), 5, 15, and 30 wt.%. Electrospinning was performed using optimized parameters and the fibers were characterized via scanning and transmission electron microscopies (SEM/TEM), energy dispersive X-ray spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), contact angle (CA), mechanical testing, antimicrobial activity against periodontopathogens, and cytotoxicity test using human dental pulp stem cells (hDPSCs). Data were analyzed using ANOVA and Tukey (alpha = 5%). Results. ZnO nanoparticles were successfully incorporated into the overall submicron fibers, which showed fairly good morphology and microstructure. Upon ZnO nanoparticies' incorporation, the PCL and PCL/GEL fibers became thicker and thinner, respectively. All GEL-containing membranes showed lower CA than the PCL-based membranes, which were highly hydrophobic. Overall, the mechanical properties of the membranes were reduced upon ZnO incorporation, except for PCL-based membranes containing ZnO at the 30 wt.% concentration. The presence of GEL enhanced the stretching ability of membranes under wet conditions. All ZnO-containing membranes displayed antibacterial activity against the bacteria tested, which was generally more pronounced with increased ZnO content. All membranes synthesized in this study demonstrated satisfactory cytocompatibility, although the presence of 30 wt.% ZnO led to decreased viability. Significance. Collectively, this study suggests that PCL- and PCL/GEL-based membranes containing a low content of ZnO nanoparticles can potentially function as a biologically safe antimicrobial GTR/GBR membrane. (C) 2015 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved. |
id |
UNSP_b647b8b18847ad41cbb6398e0c08c0ec |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/160864 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regenerationElectrospinningZinc oxideOral bacteriaPeriodontal regenerationPeriodontitisMembranesObjectives. This study reports on the synthesis, materials characterization, antimicrobial capacity, and cytocompatibility of novel ZnO-loaded membranes for guided tissue/bone regeneration (GTR/GBR). Methods. Poly(epsilon-caprolactone) (PCL) and PCL/gelatin (PCL/GEL) were dissolved in hexafluoropropanol and loaded with ZnO at distinct concentrations: 0 (control), 5, 15, and 30 wt.%. Electrospinning was performed using optimized parameters and the fibers were characterized via scanning and transmission electron microscopies (SEM/TEM), energy dispersive X-ray spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), contact angle (CA), mechanical testing, antimicrobial activity against periodontopathogens, and cytotoxicity test using human dental pulp stem cells (hDPSCs). Data were analyzed using ANOVA and Tukey (alpha = 5%). Results. ZnO nanoparticles were successfully incorporated into the overall submicron fibers, which showed fairly good morphology and microstructure. Upon ZnO nanoparticies' incorporation, the PCL and PCL/GEL fibers became thicker and thinner, respectively. All GEL-containing membranes showed lower CA than the PCL-based membranes, which were highly hydrophobic. Overall, the mechanical properties of the membranes were reduced upon ZnO incorporation, except for PCL-based membranes containing ZnO at the 30 wt.% concentration. The presence of GEL enhanced the stretching ability of membranes under wet conditions. All ZnO-containing membranes displayed antibacterial activity against the bacteria tested, which was generally more pronounced with increased ZnO content. All membranes synthesized in this study demonstrated satisfactory cytocompatibility, although the presence of 30 wt.% ZnO led to decreased viability. Significance. Collectively, this study suggests that PCL- and PCL/GEL-based membranes containing a low content of ZnO nanoparticles can potentially function as a biologically safe antimicrobial GTR/GBR membrane. (C) 2015 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.Indiana University School of DentistryNIH/NIDCRInternational Development Funds (IDF) Grant from Indiana University Purdue University (IUPUI/OVCR)Indiana Univ, Sch Dent, Dept Restorat Dent, Div Dent Biomat, Indianapolis, IN 46202 USAFed Univ Pelotas UFPEL, Dept Operat Dent, BR-96015560 Pelotas, RS, BrazilUniv Estadual Paulista, Grad Program Restorat Dent, Sao Jose dos Campos Dent Sch, BR-12245000 Sao Paulo, BrazilIUSD, Dept Oral Biol, Indianapolis, IN 46202 USAUniv Estadual Paulista, Grad Program Restorat Dent, Sao Jose dos Campos Dent Sch, BR-12245000 Sao Paulo, BrazilNIH/NIDCR: DE023552Elsevier B.V.Indiana UnivUniversidade Federal de Pernambuco (UFPE)Universidade Estadual Paulista (Unesp)IUSDMuenchow, Eliseu A.Albuquerque, Maria Tereza P. [UNESP]Zero, BiancaKamocki, KrzysztofPiva, EuandroGregory, Richard L.Bottino, Marco C.2018-11-26T16:17:03Z2018-11-26T16:17:03Z2015-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1038-1051application/pdfhttp://dx.doi.org/10.1016/j.dental.2015.06.004Dental Materials. Oxford: Elsevier Sci Ltd, v. 31, n. 9, p. 1038-1051, 2015.0109-5641http://hdl.handle.net/11449/16086410.1016/j.dental.2015.06.004WOS:000361842900006WOS000361842900006.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDental Materials2,106info:eu-repo/semantics/openAccess2023-10-12T06:08:28Zoai:repositorio.unesp.br:11449/160864Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:42:21.351116Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration |
title |
Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration |
spellingShingle |
Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration Muenchow, Eliseu A. Electrospinning Zinc oxide Oral bacteria Periodontal regeneration Periodontitis Membranes |
title_short |
Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration |
title_full |
Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration |
title_fullStr |
Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration |
title_full_unstemmed |
Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration |
title_sort |
Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration |
author |
Muenchow, Eliseu A. |
author_facet |
Muenchow, Eliseu A. Albuquerque, Maria Tereza P. [UNESP] Zero, Bianca Kamocki, Krzysztof Piva, Euandro Gregory, Richard L. Bottino, Marco C. |
author_role |
author |
author2 |
Albuquerque, Maria Tereza P. [UNESP] Zero, Bianca Kamocki, Krzysztof Piva, Euandro Gregory, Richard L. Bottino, Marco C. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Indiana Univ Universidade Federal de Pernambuco (UFPE) Universidade Estadual Paulista (Unesp) IUSD |
dc.contributor.author.fl_str_mv |
Muenchow, Eliseu A. Albuquerque, Maria Tereza P. [UNESP] Zero, Bianca Kamocki, Krzysztof Piva, Euandro Gregory, Richard L. Bottino, Marco C. |
dc.subject.por.fl_str_mv |
Electrospinning Zinc oxide Oral bacteria Periodontal regeneration Periodontitis Membranes |
topic |
Electrospinning Zinc oxide Oral bacteria Periodontal regeneration Periodontitis Membranes |
description |
Objectives. This study reports on the synthesis, materials characterization, antimicrobial capacity, and cytocompatibility of novel ZnO-loaded membranes for guided tissue/bone regeneration (GTR/GBR). Methods. Poly(epsilon-caprolactone) (PCL) and PCL/gelatin (PCL/GEL) were dissolved in hexafluoropropanol and loaded with ZnO at distinct concentrations: 0 (control), 5, 15, and 30 wt.%. Electrospinning was performed using optimized parameters and the fibers were characterized via scanning and transmission electron microscopies (SEM/TEM), energy dispersive X-ray spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), contact angle (CA), mechanical testing, antimicrobial activity against periodontopathogens, and cytotoxicity test using human dental pulp stem cells (hDPSCs). Data were analyzed using ANOVA and Tukey (alpha = 5%). Results. ZnO nanoparticles were successfully incorporated into the overall submicron fibers, which showed fairly good morphology and microstructure. Upon ZnO nanoparticies' incorporation, the PCL and PCL/GEL fibers became thicker and thinner, respectively. All GEL-containing membranes showed lower CA than the PCL-based membranes, which were highly hydrophobic. Overall, the mechanical properties of the membranes were reduced upon ZnO incorporation, except for PCL-based membranes containing ZnO at the 30 wt.% concentration. The presence of GEL enhanced the stretching ability of membranes under wet conditions. All ZnO-containing membranes displayed antibacterial activity against the bacteria tested, which was generally more pronounced with increased ZnO content. All membranes synthesized in this study demonstrated satisfactory cytocompatibility, although the presence of 30 wt.% ZnO led to decreased viability. Significance. Collectively, this study suggests that PCL- and PCL/GEL-based membranes containing a low content of ZnO nanoparticles can potentially function as a biologically safe antimicrobial GTR/GBR membrane. (C) 2015 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-09-01 2018-11-26T16:17:03Z 2018-11-26T16:17:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.dental.2015.06.004 Dental Materials. Oxford: Elsevier Sci Ltd, v. 31, n. 9, p. 1038-1051, 2015. 0109-5641 http://hdl.handle.net/11449/160864 10.1016/j.dental.2015.06.004 WOS:000361842900006 WOS000361842900006.pdf |
url |
http://dx.doi.org/10.1016/j.dental.2015.06.004 http://hdl.handle.net/11449/160864 |
identifier_str_mv |
Dental Materials. Oxford: Elsevier Sci Ltd, v. 31, n. 9, p. 1038-1051, 2015. 0109-5641 10.1016/j.dental.2015.06.004 WOS:000361842900006 WOS000361842900006.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Dental Materials 2,106 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1038-1051 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128405355364352 |