Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration

Detalhes bibliográficos
Autor(a) principal: Muenchow, Eliseu A.
Data de Publicação: 2015
Outros Autores: Albuquerque, Maria Tereza P. [UNESP], Zero, Bianca, Kamocki, Krzysztof, Piva, Euandro, Gregory, Richard L., Bottino, Marco C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.dental.2015.06.004
http://hdl.handle.net/11449/160864
Resumo: Objectives. This study reports on the synthesis, materials characterization, antimicrobial capacity, and cytocompatibility of novel ZnO-loaded membranes for guided tissue/bone regeneration (GTR/GBR). Methods. Poly(epsilon-caprolactone) (PCL) and PCL/gelatin (PCL/GEL) were dissolved in hexafluoropropanol and loaded with ZnO at distinct concentrations: 0 (control), 5, 15, and 30 wt.%. Electrospinning was performed using optimized parameters and the fibers were characterized via scanning and transmission electron microscopies (SEM/TEM), energy dispersive X-ray spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), contact angle (CA), mechanical testing, antimicrobial activity against periodontopathogens, and cytotoxicity test using human dental pulp stem cells (hDPSCs). Data were analyzed using ANOVA and Tukey (alpha = 5%). Results. ZnO nanoparticles were successfully incorporated into the overall submicron fibers, which showed fairly good morphology and microstructure. Upon ZnO nanoparticies' incorporation, the PCL and PCL/GEL fibers became thicker and thinner, respectively. All GEL-containing membranes showed lower CA than the PCL-based membranes, which were highly hydrophobic. Overall, the mechanical properties of the membranes were reduced upon ZnO incorporation, except for PCL-based membranes containing ZnO at the 30 wt.% concentration. The presence of GEL enhanced the stretching ability of membranes under wet conditions. All ZnO-containing membranes displayed antibacterial activity against the bacteria tested, which was generally more pronounced with increased ZnO content. All membranes synthesized in this study demonstrated satisfactory cytocompatibility, although the presence of 30 wt.% ZnO led to decreased viability. Significance. Collectively, this study suggests that PCL- and PCL/GEL-based membranes containing a low content of ZnO nanoparticles can potentially function as a biologically safe antimicrobial GTR/GBR membrane. (C) 2015 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
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spelling Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regenerationElectrospinningZinc oxideOral bacteriaPeriodontal regenerationPeriodontitisMembranesObjectives. This study reports on the synthesis, materials characterization, antimicrobial capacity, and cytocompatibility of novel ZnO-loaded membranes for guided tissue/bone regeneration (GTR/GBR). Methods. Poly(epsilon-caprolactone) (PCL) and PCL/gelatin (PCL/GEL) were dissolved in hexafluoropropanol and loaded with ZnO at distinct concentrations: 0 (control), 5, 15, and 30 wt.%. Electrospinning was performed using optimized parameters and the fibers were characterized via scanning and transmission electron microscopies (SEM/TEM), energy dispersive X-ray spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), contact angle (CA), mechanical testing, antimicrobial activity against periodontopathogens, and cytotoxicity test using human dental pulp stem cells (hDPSCs). Data were analyzed using ANOVA and Tukey (alpha = 5%). Results. ZnO nanoparticles were successfully incorporated into the overall submicron fibers, which showed fairly good morphology and microstructure. Upon ZnO nanoparticies' incorporation, the PCL and PCL/GEL fibers became thicker and thinner, respectively. All GEL-containing membranes showed lower CA than the PCL-based membranes, which were highly hydrophobic. Overall, the mechanical properties of the membranes were reduced upon ZnO incorporation, except for PCL-based membranes containing ZnO at the 30 wt.% concentration. The presence of GEL enhanced the stretching ability of membranes under wet conditions. All ZnO-containing membranes displayed antibacterial activity against the bacteria tested, which was generally more pronounced with increased ZnO content. All membranes synthesized in this study demonstrated satisfactory cytocompatibility, although the presence of 30 wt.% ZnO led to decreased viability. Significance. Collectively, this study suggests that PCL- and PCL/GEL-based membranes containing a low content of ZnO nanoparticles can potentially function as a biologically safe antimicrobial GTR/GBR membrane. (C) 2015 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.Indiana University School of DentistryNIH/NIDCRInternational Development Funds (IDF) Grant from Indiana University Purdue University (IUPUI/OVCR)Indiana Univ, Sch Dent, Dept Restorat Dent, Div Dent Biomat, Indianapolis, IN 46202 USAFed Univ Pelotas UFPEL, Dept Operat Dent, BR-96015560 Pelotas, RS, BrazilUniv Estadual Paulista, Grad Program Restorat Dent, Sao Jose dos Campos Dent Sch, BR-12245000 Sao Paulo, BrazilIUSD, Dept Oral Biol, Indianapolis, IN 46202 USAUniv Estadual Paulista, Grad Program Restorat Dent, Sao Jose dos Campos Dent Sch, BR-12245000 Sao Paulo, BrazilNIH/NIDCR: DE023552Elsevier B.V.Indiana UnivUniversidade Federal de Pernambuco (UFPE)Universidade Estadual Paulista (Unesp)IUSDMuenchow, Eliseu A.Albuquerque, Maria Tereza P. [UNESP]Zero, BiancaKamocki, KrzysztofPiva, EuandroGregory, Richard L.Bottino, Marco C.2018-11-26T16:17:03Z2018-11-26T16:17:03Z2015-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1038-1051application/pdfhttp://dx.doi.org/10.1016/j.dental.2015.06.004Dental Materials. Oxford: Elsevier Sci Ltd, v. 31, n. 9, p. 1038-1051, 2015.0109-5641http://hdl.handle.net/11449/16086410.1016/j.dental.2015.06.004WOS:000361842900006WOS000361842900006.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDental Materials2,106info:eu-repo/semantics/openAccess2023-10-12T06:08:28Zoai:repositorio.unesp.br:11449/160864Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:42:21.351116Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration
title Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration
spellingShingle Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration
Muenchow, Eliseu A.
Electrospinning
Zinc oxide
Oral bacteria
Periodontal regeneration
Periodontitis
Membranes
title_short Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration
title_full Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration
title_fullStr Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration
title_full_unstemmed Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration
title_sort Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration
author Muenchow, Eliseu A.
author_facet Muenchow, Eliseu A.
Albuquerque, Maria Tereza P. [UNESP]
Zero, Bianca
Kamocki, Krzysztof
Piva, Euandro
Gregory, Richard L.
Bottino, Marco C.
author_role author
author2 Albuquerque, Maria Tereza P. [UNESP]
Zero, Bianca
Kamocki, Krzysztof
Piva, Euandro
Gregory, Richard L.
Bottino, Marco C.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Indiana Univ
Universidade Federal de Pernambuco (UFPE)
Universidade Estadual Paulista (Unesp)
IUSD
dc.contributor.author.fl_str_mv Muenchow, Eliseu A.
Albuquerque, Maria Tereza P. [UNESP]
Zero, Bianca
Kamocki, Krzysztof
Piva, Euandro
Gregory, Richard L.
Bottino, Marco C.
dc.subject.por.fl_str_mv Electrospinning
Zinc oxide
Oral bacteria
Periodontal regeneration
Periodontitis
Membranes
topic Electrospinning
Zinc oxide
Oral bacteria
Periodontal regeneration
Periodontitis
Membranes
description Objectives. This study reports on the synthesis, materials characterization, antimicrobial capacity, and cytocompatibility of novel ZnO-loaded membranes for guided tissue/bone regeneration (GTR/GBR). Methods. Poly(epsilon-caprolactone) (PCL) and PCL/gelatin (PCL/GEL) were dissolved in hexafluoropropanol and loaded with ZnO at distinct concentrations: 0 (control), 5, 15, and 30 wt.%. Electrospinning was performed using optimized parameters and the fibers were characterized via scanning and transmission electron microscopies (SEM/TEM), energy dispersive X-ray spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), contact angle (CA), mechanical testing, antimicrobial activity against periodontopathogens, and cytotoxicity test using human dental pulp stem cells (hDPSCs). Data were analyzed using ANOVA and Tukey (alpha = 5%). Results. ZnO nanoparticles were successfully incorporated into the overall submicron fibers, which showed fairly good morphology and microstructure. Upon ZnO nanoparticies' incorporation, the PCL and PCL/GEL fibers became thicker and thinner, respectively. All GEL-containing membranes showed lower CA than the PCL-based membranes, which were highly hydrophobic. Overall, the mechanical properties of the membranes were reduced upon ZnO incorporation, except for PCL-based membranes containing ZnO at the 30 wt.% concentration. The presence of GEL enhanced the stretching ability of membranes under wet conditions. All ZnO-containing membranes displayed antibacterial activity against the bacteria tested, which was generally more pronounced with increased ZnO content. All membranes synthesized in this study demonstrated satisfactory cytocompatibility, although the presence of 30 wt.% ZnO led to decreased viability. Significance. Collectively, this study suggests that PCL- and PCL/GEL-based membranes containing a low content of ZnO nanoparticles can potentially function as a biologically safe antimicrobial GTR/GBR membrane. (C) 2015 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
publishDate 2015
dc.date.none.fl_str_mv 2015-09-01
2018-11-26T16:17:03Z
2018-11-26T16:17:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.dental.2015.06.004
Dental Materials. Oxford: Elsevier Sci Ltd, v. 31, n. 9, p. 1038-1051, 2015.
0109-5641
http://hdl.handle.net/11449/160864
10.1016/j.dental.2015.06.004
WOS:000361842900006
WOS000361842900006.pdf
url http://dx.doi.org/10.1016/j.dental.2015.06.004
http://hdl.handle.net/11449/160864
identifier_str_mv Dental Materials. Oxford: Elsevier Sci Ltd, v. 31, n. 9, p. 1038-1051, 2015.
0109-5641
10.1016/j.dental.2015.06.004
WOS:000361842900006
WOS000361842900006.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Dental Materials
2,106
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1038-1051
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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