The Effect of Sub-Lethal Successive Applications of Photodynamic Therapy on Candida albicans Biofilm Depends on the Photosensitizer
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/jof9010111 http://hdl.handle.net/11449/249592 |
Resumo: | This study aimed to evaluate the potential of successive applications of sub-lethal doses of the antimicrobial photodynamic therapy (aPDT) mediated by Photodithazine® (PDZ) and curcumin (CUR) associated with LED in the viability, reactive oxygen species (ROS) production, and gene expression of Candida albicans. The microbial assays were performed using planktonic cultures and biofilms. Ten successive applications (Apl#) were performed: aPDT (P+L+; C+L+), photosensitizer (P+L−; C+L−), and LED (P−L+; C−L+). Control groups were used (P−L−; C−L−). The viability of C. albicans was determined by cultivating treated cultures on agar plates with or without fluconazole (FLU). In addition, the ROS detection and expression of SOD1, CAP1, and ERG11 genes were determined. For planktonic cultures, no viable colonies were observed after Apl#3 (without FLU) and Apl#2 (with FLU) for either photosensitizer. Biofilm treated with P+L+ resulted in the absence of cell viability after Apl#7, while C+L+ showed ~1.40 log10 increase in cell viability after Apl#2, regardless of FLU. For both photosensitizers, after the last application with viable colonies, the production of ROS was higher in the biofilms than in the planktonic cultures, and SOD1 expression was the highest in P+L+. A reduction of CAP1 and ERG11 expression occurred after P+L+, regardless of FLU. C+L+ had a higher level of ROS, and the treatments were non-significant for gene expression. Sub-lethal doses of aPDT mediated by CUR could induce C. albicans resistance in biofilms, while C. albicans cells in biofilms were susceptible to aPDT mediated by PDZ. |
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The Effect of Sub-Lethal Successive Applications of Photodynamic Therapy on Candida albicans Biofilm Depends on the PhotosensitizerCandida albicansfluconazolephotodynamic therapyThis study aimed to evaluate the potential of successive applications of sub-lethal doses of the antimicrobial photodynamic therapy (aPDT) mediated by Photodithazine® (PDZ) and curcumin (CUR) associated with LED in the viability, reactive oxygen species (ROS) production, and gene expression of Candida albicans. The microbial assays were performed using planktonic cultures and biofilms. Ten successive applications (Apl#) were performed: aPDT (P+L+; C+L+), photosensitizer (P+L−; C+L−), and LED (P−L+; C−L+). Control groups were used (P−L−; C−L−). The viability of C. albicans was determined by cultivating treated cultures on agar plates with or without fluconazole (FLU). In addition, the ROS detection and expression of SOD1, CAP1, and ERG11 genes were determined. For planktonic cultures, no viable colonies were observed after Apl#3 (without FLU) and Apl#2 (with FLU) for either photosensitizer. Biofilm treated with P+L+ resulted in the absence of cell viability after Apl#7, while C+L+ showed ~1.40 log10 increase in cell viability after Apl#2, regardless of FLU. For both photosensitizers, after the last application with viable colonies, the production of ROS was higher in the biofilms than in the planktonic cultures, and SOD1 expression was the highest in P+L+. A reduction of CAP1 and ERG11 expression occurred after P+L+, regardless of FLU. C+L+ had a higher level of ROS, and the treatments were non-significant for gene expression. Sub-lethal doses of aPDT mediated by CUR could induce C. albicans resistance in biofilms, while C. albicans cells in biofilms were susceptible to aPDT mediated by PDZ.Forest Products Research and Development InstituteDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)Forest Products Research and Development Institute: #304133/2016-7Universidade Estadual Paulista (UNESP)Dias, Luana Mendonça [UNESP]Klein, Marlise Inêz [UNESP]Ferrisse, Túlio Morandin [UNESP]Medeiros, Karine Sousa [UNESP]Jordão, Cláudia Carolina [UNESP]Bellini, Amanda [UNESP]Pavarina, Ana Claudia [UNESP]2023-07-29T16:03:55Z2023-07-29T16:03:55Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/jof9010111Journal of Fungi, v. 9, n. 1, 2023.2309-608Xhttp://hdl.handle.net/11449/24959210.3390/jof90101112-s2.0-85146759402Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Fungiinfo:eu-repo/semantics/openAccess2024-09-27T14:57:27Zoai:repositorio.unesp.br:11449/249592Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:57:27Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The Effect of Sub-Lethal Successive Applications of Photodynamic Therapy on Candida albicans Biofilm Depends on the Photosensitizer |
title |
The Effect of Sub-Lethal Successive Applications of Photodynamic Therapy on Candida albicans Biofilm Depends on the Photosensitizer |
spellingShingle |
The Effect of Sub-Lethal Successive Applications of Photodynamic Therapy on Candida albicans Biofilm Depends on the Photosensitizer Dias, Luana Mendonça [UNESP] Candida albicans fluconazole photodynamic therapy |
title_short |
The Effect of Sub-Lethal Successive Applications of Photodynamic Therapy on Candida albicans Biofilm Depends on the Photosensitizer |
title_full |
The Effect of Sub-Lethal Successive Applications of Photodynamic Therapy on Candida albicans Biofilm Depends on the Photosensitizer |
title_fullStr |
The Effect of Sub-Lethal Successive Applications of Photodynamic Therapy on Candida albicans Biofilm Depends on the Photosensitizer |
title_full_unstemmed |
The Effect of Sub-Lethal Successive Applications of Photodynamic Therapy on Candida albicans Biofilm Depends on the Photosensitizer |
title_sort |
The Effect of Sub-Lethal Successive Applications of Photodynamic Therapy on Candida albicans Biofilm Depends on the Photosensitizer |
author |
Dias, Luana Mendonça [UNESP] |
author_facet |
Dias, Luana Mendonça [UNESP] Klein, Marlise Inêz [UNESP] Ferrisse, Túlio Morandin [UNESP] Medeiros, Karine Sousa [UNESP] Jordão, Cláudia Carolina [UNESP] Bellini, Amanda [UNESP] Pavarina, Ana Claudia [UNESP] |
author_role |
author |
author2 |
Klein, Marlise Inêz [UNESP] Ferrisse, Túlio Morandin [UNESP] Medeiros, Karine Sousa [UNESP] Jordão, Cláudia Carolina [UNESP] Bellini, Amanda [UNESP] Pavarina, Ana Claudia [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Dias, Luana Mendonça [UNESP] Klein, Marlise Inêz [UNESP] Ferrisse, Túlio Morandin [UNESP] Medeiros, Karine Sousa [UNESP] Jordão, Cláudia Carolina [UNESP] Bellini, Amanda [UNESP] Pavarina, Ana Claudia [UNESP] |
dc.subject.por.fl_str_mv |
Candida albicans fluconazole photodynamic therapy |
topic |
Candida albicans fluconazole photodynamic therapy |
description |
This study aimed to evaluate the potential of successive applications of sub-lethal doses of the antimicrobial photodynamic therapy (aPDT) mediated by Photodithazine® (PDZ) and curcumin (CUR) associated with LED in the viability, reactive oxygen species (ROS) production, and gene expression of Candida albicans. The microbial assays were performed using planktonic cultures and biofilms. Ten successive applications (Apl#) were performed: aPDT (P+L+; C+L+), photosensitizer (P+L−; C+L−), and LED (P−L+; C−L+). Control groups were used (P−L−; C−L−). The viability of C. albicans was determined by cultivating treated cultures on agar plates with or without fluconazole (FLU). In addition, the ROS detection and expression of SOD1, CAP1, and ERG11 genes were determined. For planktonic cultures, no viable colonies were observed after Apl#3 (without FLU) and Apl#2 (with FLU) for either photosensitizer. Biofilm treated with P+L+ resulted in the absence of cell viability after Apl#7, while C+L+ showed ~1.40 log10 increase in cell viability after Apl#2, regardless of FLU. For both photosensitizers, after the last application with viable colonies, the production of ROS was higher in the biofilms than in the planktonic cultures, and SOD1 expression was the highest in P+L+. A reduction of CAP1 and ERG11 expression occurred after P+L+, regardless of FLU. C+L+ had a higher level of ROS, and the treatments were non-significant for gene expression. Sub-lethal doses of aPDT mediated by CUR could induce C. albicans resistance in biofilms, while C. albicans cells in biofilms were susceptible to aPDT mediated by PDZ. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T16:03:55Z 2023-07-29T16:03:55Z 2023-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/jof9010111 Journal of Fungi, v. 9, n. 1, 2023. 2309-608X http://hdl.handle.net/11449/249592 10.3390/jof9010111 2-s2.0-85146759402 |
url |
http://dx.doi.org/10.3390/jof9010111 http://hdl.handle.net/11449/249592 |
identifier_str_mv |
Journal of Fungi, v. 9, n. 1, 2023. 2309-608X 10.3390/jof9010111 2-s2.0-85146759402 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Fungi |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546521916866560 |