The Effect of Sub-Lethal Successive Applications of Photodynamic Therapy on Candida albicans Biofilm Depends on the Photosensitizer

Detalhes bibliográficos
Autor(a) principal: Dias, Luana Mendonça [UNESP]
Data de Publicação: 2023
Outros Autores: Klein, Marlise Inêz [UNESP], Ferrisse, Túlio Morandin [UNESP], Medeiros, Karine Sousa [UNESP], Jordão, Cláudia Carolina [UNESP], Bellini, Amanda [UNESP], Pavarina, Ana Claudia [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/jof9010111
http://hdl.handle.net/11449/249592
Resumo: This study aimed to evaluate the potential of successive applications of sub-lethal doses of the antimicrobial photodynamic therapy (aPDT) mediated by Photodithazine® (PDZ) and curcumin (CUR) associated with LED in the viability, reactive oxygen species (ROS) production, and gene expression of Candida albicans. The microbial assays were performed using planktonic cultures and biofilms. Ten successive applications (Apl#) were performed: aPDT (P+L+; C+L+), photosensitizer (P+L−; C+L−), and LED (P−L+; C−L+). Control groups were used (P−L−; C−L−). The viability of C. albicans was determined by cultivating treated cultures on agar plates with or without fluconazole (FLU). In addition, the ROS detection and expression of SOD1, CAP1, and ERG11 genes were determined. For planktonic cultures, no viable colonies were observed after Apl#3 (without FLU) and Apl#2 (with FLU) for either photosensitizer. Biofilm treated with P+L+ resulted in the absence of cell viability after Apl#7, while C+L+ showed ~1.40 log10 increase in cell viability after Apl#2, regardless of FLU. For both photosensitizers, after the last application with viable colonies, the production of ROS was higher in the biofilms than in the planktonic cultures, and SOD1 expression was the highest in P+L+. A reduction of CAP1 and ERG11 expression occurred after P+L+, regardless of FLU. C+L+ had a higher level of ROS, and the treatments were non-significant for gene expression. Sub-lethal doses of aPDT mediated by CUR could induce C. albicans resistance in biofilms, while C. albicans cells in biofilms were susceptible to aPDT mediated by PDZ.
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spelling The Effect of Sub-Lethal Successive Applications of Photodynamic Therapy on Candida albicans Biofilm Depends on the PhotosensitizerCandida albicansfluconazolephotodynamic therapyThis study aimed to evaluate the potential of successive applications of sub-lethal doses of the antimicrobial photodynamic therapy (aPDT) mediated by Photodithazine® (PDZ) and curcumin (CUR) associated with LED in the viability, reactive oxygen species (ROS) production, and gene expression of Candida albicans. The microbial assays were performed using planktonic cultures and biofilms. Ten successive applications (Apl#) were performed: aPDT (P+L+; C+L+), photosensitizer (P+L−; C+L−), and LED (P−L+; C−L+). Control groups were used (P−L−; C−L−). The viability of C. albicans was determined by cultivating treated cultures on agar plates with or without fluconazole (FLU). In addition, the ROS detection and expression of SOD1, CAP1, and ERG11 genes were determined. For planktonic cultures, no viable colonies were observed after Apl#3 (without FLU) and Apl#2 (with FLU) for either photosensitizer. Biofilm treated with P+L+ resulted in the absence of cell viability after Apl#7, while C+L+ showed ~1.40 log10 increase in cell viability after Apl#2, regardless of FLU. For both photosensitizers, after the last application with viable colonies, the production of ROS was higher in the biofilms than in the planktonic cultures, and SOD1 expression was the highest in P+L+. A reduction of CAP1 and ERG11 expression occurred after P+L+, regardless of FLU. C+L+ had a higher level of ROS, and the treatments were non-significant for gene expression. Sub-lethal doses of aPDT mediated by CUR could induce C. albicans resistance in biofilms, while C. albicans cells in biofilms were susceptible to aPDT mediated by PDZ.Forest Products Research and Development InstituteDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)Forest Products Research and Development Institute: #304133/2016-7Universidade Estadual Paulista (UNESP)Dias, Luana Mendonça [UNESP]Klein, Marlise Inêz [UNESP]Ferrisse, Túlio Morandin [UNESP]Medeiros, Karine Sousa [UNESP]Jordão, Cláudia Carolina [UNESP]Bellini, Amanda [UNESP]Pavarina, Ana Claudia [UNESP]2023-07-29T16:03:55Z2023-07-29T16:03:55Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/jof9010111Journal of Fungi, v. 9, n. 1, 2023.2309-608Xhttp://hdl.handle.net/11449/24959210.3390/jof90101112-s2.0-85146759402Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Fungiinfo:eu-repo/semantics/openAccess2024-09-27T14:57:27Zoai:repositorio.unesp.br:11449/249592Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:57:27Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv The Effect of Sub-Lethal Successive Applications of Photodynamic Therapy on Candida albicans Biofilm Depends on the Photosensitizer
title The Effect of Sub-Lethal Successive Applications of Photodynamic Therapy on Candida albicans Biofilm Depends on the Photosensitizer
spellingShingle The Effect of Sub-Lethal Successive Applications of Photodynamic Therapy on Candida albicans Biofilm Depends on the Photosensitizer
Dias, Luana Mendonça [UNESP]
Candida albicans
fluconazole
photodynamic therapy
title_short The Effect of Sub-Lethal Successive Applications of Photodynamic Therapy on Candida albicans Biofilm Depends on the Photosensitizer
title_full The Effect of Sub-Lethal Successive Applications of Photodynamic Therapy on Candida albicans Biofilm Depends on the Photosensitizer
title_fullStr The Effect of Sub-Lethal Successive Applications of Photodynamic Therapy on Candida albicans Biofilm Depends on the Photosensitizer
title_full_unstemmed The Effect of Sub-Lethal Successive Applications of Photodynamic Therapy on Candida albicans Biofilm Depends on the Photosensitizer
title_sort The Effect of Sub-Lethal Successive Applications of Photodynamic Therapy on Candida albicans Biofilm Depends on the Photosensitizer
author Dias, Luana Mendonça [UNESP]
author_facet Dias, Luana Mendonça [UNESP]
Klein, Marlise Inêz [UNESP]
Ferrisse, Túlio Morandin [UNESP]
Medeiros, Karine Sousa [UNESP]
Jordão, Cláudia Carolina [UNESP]
Bellini, Amanda [UNESP]
Pavarina, Ana Claudia [UNESP]
author_role author
author2 Klein, Marlise Inêz [UNESP]
Ferrisse, Túlio Morandin [UNESP]
Medeiros, Karine Sousa [UNESP]
Jordão, Cláudia Carolina [UNESP]
Bellini, Amanda [UNESP]
Pavarina, Ana Claudia [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Dias, Luana Mendonça [UNESP]
Klein, Marlise Inêz [UNESP]
Ferrisse, Túlio Morandin [UNESP]
Medeiros, Karine Sousa [UNESP]
Jordão, Cláudia Carolina [UNESP]
Bellini, Amanda [UNESP]
Pavarina, Ana Claudia [UNESP]
dc.subject.por.fl_str_mv Candida albicans
fluconazole
photodynamic therapy
topic Candida albicans
fluconazole
photodynamic therapy
description This study aimed to evaluate the potential of successive applications of sub-lethal doses of the antimicrobial photodynamic therapy (aPDT) mediated by Photodithazine® (PDZ) and curcumin (CUR) associated with LED in the viability, reactive oxygen species (ROS) production, and gene expression of Candida albicans. The microbial assays were performed using planktonic cultures and biofilms. Ten successive applications (Apl#) were performed: aPDT (P+L+; C+L+), photosensitizer (P+L−; C+L−), and LED (P−L+; C−L+). Control groups were used (P−L−; C−L−). The viability of C. albicans was determined by cultivating treated cultures on agar plates with or without fluconazole (FLU). In addition, the ROS detection and expression of SOD1, CAP1, and ERG11 genes were determined. For planktonic cultures, no viable colonies were observed after Apl#3 (without FLU) and Apl#2 (with FLU) for either photosensitizer. Biofilm treated with P+L+ resulted in the absence of cell viability after Apl#7, while C+L+ showed ~1.40 log10 increase in cell viability after Apl#2, regardless of FLU. For both photosensitizers, after the last application with viable colonies, the production of ROS was higher in the biofilms than in the planktonic cultures, and SOD1 expression was the highest in P+L+. A reduction of CAP1 and ERG11 expression occurred after P+L+, regardless of FLU. C+L+ had a higher level of ROS, and the treatments were non-significant for gene expression. Sub-lethal doses of aPDT mediated by CUR could induce C. albicans resistance in biofilms, while C. albicans cells in biofilms were susceptible to aPDT mediated by PDZ.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T16:03:55Z
2023-07-29T16:03:55Z
2023-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/jof9010111
Journal of Fungi, v. 9, n. 1, 2023.
2309-608X
http://hdl.handle.net/11449/249592
10.3390/jof9010111
2-s2.0-85146759402
url http://dx.doi.org/10.3390/jof9010111
http://hdl.handle.net/11449/249592
identifier_str_mv Journal of Fungi, v. 9, n. 1, 2023.
2309-608X
10.3390/jof9010111
2-s2.0-85146759402
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Fungi
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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