Successive applications of Antimicrobial Photodynamic Therapy effects the susceptibility of Candida albicans grown in medium with or without fluconazole

Detalhes bibliográficos
Autor(a) principal: Dias, Luana Mendonça [UNESP]
Data de Publicação: 2020
Outros Autores: Klein, Marlise Inêz [UNESP], Jordão, Cláudia Carolina [UNESP], Carmello, Juliana Cabrini [UNESP], Bellini, Amanda [UNESP], Pavarina, Ana Cláudia [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.pdpdt.2020.102018
http://hdl.handle.net/11449/206652
Resumo: Antimicrobial Photodynamic Therapy (aPDT) was introduced as a therapy due to resistance that microorganisms have developed to conventional drugs. The study aimed to evaluate the potential of successive applications of aPDT in effecting Candida albicans susceptibility and also whether the presence of fluconazole effected the recovery of the fungi in the culture medium. Planktonic cultures and biofilm were subjected to successive applications of Photodithazine-mediated (25 mg/L) LED-associated aPDT (660 nm, 34 mW/cm2). Plating was performed on Sabouraud Dextrose Agar supplemented or not with fluconazole to recover colony-forming units per milliliter (CFU/mL). Surviving cells were recovered, recultivated, and again exposed to the treatment. The treatments were performed until not enough colonies were available for recultivation and continuation of the protocol. The complete inactivation of the fungus was obtained after three and five applications for planktonic culture and biofilm, respectively. A reduction of 6.3 log10 was observed after third applications in the planktonic cultures grown on medium without fluconazole, while there was a 7 log10 reduction of these cultures grown on fluconazole medium. However, a reduction of 6.1 log10 occurred for biofilms after fifth applications for cultures grown on medium without fluconazole, while a reduction of 6.7 log10 was observed for cultures grown on medium with the antifungal. Thus, aPDT was potentiated by fluconazole. C. albicans in planktonic and biofilm cultures are susceptible to successive applications of PDZ-mediated aPDT, and tolerance to aPDT is higher in the biofilm.
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spelling Successive applications of Antimicrobial Photodynamic Therapy effects the susceptibility of Candida albicans grown in medium with or without fluconazoleAntimicrobial photodynamic therapyCandida albicansDrug resistanceAntimicrobial Photodynamic Therapy (aPDT) was introduced as a therapy due to resistance that microorganisms have developed to conventional drugs. The study aimed to evaluate the potential of successive applications of aPDT in effecting Candida albicans susceptibility and also whether the presence of fluconazole effected the recovery of the fungi in the culture medium. Planktonic cultures and biofilm were subjected to successive applications of Photodithazine-mediated (25 mg/L) LED-associated aPDT (660 nm, 34 mW/cm2). Plating was performed on Sabouraud Dextrose Agar supplemented or not with fluconazole to recover colony-forming units per milliliter (CFU/mL). Surviving cells were recovered, recultivated, and again exposed to the treatment. The treatments were performed until not enough colonies were available for recultivation and continuation of the protocol. The complete inactivation of the fungus was obtained after three and five applications for planktonic culture and biofilm, respectively. A reduction of 6.3 log10 was observed after third applications in the planktonic cultures grown on medium without fluconazole, while there was a 7 log10 reduction of these cultures grown on fluconazole medium. However, a reduction of 6.1 log10 occurred for biofilms after fifth applications for cultures grown on medium without fluconazole, while a reduction of 6.7 log10 was observed for cultures grown on medium with the antifungal. Thus, aPDT was potentiated by fluconazole. C. albicans in planktonic and biofilm cultures are susceptible to successive applications of PDZ-mediated aPDT, and tolerance to aPDT is higher in the biofilm.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Dental Materials and Prosthodontics Sao Paulo State University (UNESP) School of Dentistry, Humaitá Street, 1680, 14801-903Department of Dental Materials and Prosthodontics Sao Paulo State University (UNESP) School of Dentistry, Humaitá Street, 1680, 14801-903FAPESP: 13-07276-1FAPESP: 2016/13868-7FAPESP: 2017-02559-6FAPESP: 2018/14874-6Universidade Estadual Paulista (Unesp)Dias, Luana Mendonça [UNESP]Klein, Marlise Inêz [UNESP]Jordão, Cláudia Carolina [UNESP]Carmello, Juliana Cabrini [UNESP]Bellini, Amanda [UNESP]Pavarina, Ana Cláudia [UNESP]2021-06-25T10:35:52Z2021-06-25T10:35:52Z2020-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.pdpdt.2020.102018Photodiagnosis and Photodynamic Therapy, v. 32.1873-15971572-1000http://hdl.handle.net/11449/20665210.1016/j.pdpdt.2020.1020182-s2.0-85092455181Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotodiagnosis and Photodynamic Therapyinfo:eu-repo/semantics/openAccess2021-10-23T08:32:08Zoai:repositorio.unesp.br:11449/206652Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T08:32:08Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Successive applications of Antimicrobial Photodynamic Therapy effects the susceptibility of Candida albicans grown in medium with or without fluconazole
title Successive applications of Antimicrobial Photodynamic Therapy effects the susceptibility of Candida albicans grown in medium with or without fluconazole
spellingShingle Successive applications of Antimicrobial Photodynamic Therapy effects the susceptibility of Candida albicans grown in medium with or without fluconazole
Dias, Luana Mendonça [UNESP]
Antimicrobial photodynamic therapy
Candida albicans
Drug resistance
title_short Successive applications of Antimicrobial Photodynamic Therapy effects the susceptibility of Candida albicans grown in medium with or without fluconazole
title_full Successive applications of Antimicrobial Photodynamic Therapy effects the susceptibility of Candida albicans grown in medium with or without fluconazole
title_fullStr Successive applications of Antimicrobial Photodynamic Therapy effects the susceptibility of Candida albicans grown in medium with or without fluconazole
title_full_unstemmed Successive applications of Antimicrobial Photodynamic Therapy effects the susceptibility of Candida albicans grown in medium with or without fluconazole
title_sort Successive applications of Antimicrobial Photodynamic Therapy effects the susceptibility of Candida albicans grown in medium with or without fluconazole
author Dias, Luana Mendonça [UNESP]
author_facet Dias, Luana Mendonça [UNESP]
Klein, Marlise Inêz [UNESP]
Jordão, Cláudia Carolina [UNESP]
Carmello, Juliana Cabrini [UNESP]
Bellini, Amanda [UNESP]
Pavarina, Ana Cláudia [UNESP]
author_role author
author2 Klein, Marlise Inêz [UNESP]
Jordão, Cláudia Carolina [UNESP]
Carmello, Juliana Cabrini [UNESP]
Bellini, Amanda [UNESP]
Pavarina, Ana Cláudia [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Dias, Luana Mendonça [UNESP]
Klein, Marlise Inêz [UNESP]
Jordão, Cláudia Carolina [UNESP]
Carmello, Juliana Cabrini [UNESP]
Bellini, Amanda [UNESP]
Pavarina, Ana Cláudia [UNESP]
dc.subject.por.fl_str_mv Antimicrobial photodynamic therapy
Candida albicans
Drug resistance
topic Antimicrobial photodynamic therapy
Candida albicans
Drug resistance
description Antimicrobial Photodynamic Therapy (aPDT) was introduced as a therapy due to resistance that microorganisms have developed to conventional drugs. The study aimed to evaluate the potential of successive applications of aPDT in effecting Candida albicans susceptibility and also whether the presence of fluconazole effected the recovery of the fungi in the culture medium. Planktonic cultures and biofilm were subjected to successive applications of Photodithazine-mediated (25 mg/L) LED-associated aPDT (660 nm, 34 mW/cm2). Plating was performed on Sabouraud Dextrose Agar supplemented or not with fluconazole to recover colony-forming units per milliliter (CFU/mL). Surviving cells were recovered, recultivated, and again exposed to the treatment. The treatments were performed until not enough colonies were available for recultivation and continuation of the protocol. The complete inactivation of the fungus was obtained after three and five applications for planktonic culture and biofilm, respectively. A reduction of 6.3 log10 was observed after third applications in the planktonic cultures grown on medium without fluconazole, while there was a 7 log10 reduction of these cultures grown on fluconazole medium. However, a reduction of 6.1 log10 occurred for biofilms after fifth applications for cultures grown on medium without fluconazole, while a reduction of 6.7 log10 was observed for cultures grown on medium with the antifungal. Thus, aPDT was potentiated by fluconazole. C. albicans in planktonic and biofilm cultures are susceptible to successive applications of PDZ-mediated aPDT, and tolerance to aPDT is higher in the biofilm.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-01
2021-06-25T10:35:52Z
2021-06-25T10:35:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.pdpdt.2020.102018
Photodiagnosis and Photodynamic Therapy, v. 32.
1873-1597
1572-1000
http://hdl.handle.net/11449/206652
10.1016/j.pdpdt.2020.102018
2-s2.0-85092455181
url http://dx.doi.org/10.1016/j.pdpdt.2020.102018
http://hdl.handle.net/11449/206652
identifier_str_mv Photodiagnosis and Photodynamic Therapy, v. 32.
1873-1597
1572-1000
10.1016/j.pdpdt.2020.102018
2-s2.0-85092455181
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Photodiagnosis and Photodynamic Therapy
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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