High frequency of genital carriers of Leptospira sp. in sheep slaughtered in the semi-arid region of northeastern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11250-018-1657-9 http://hdl.handle.net/11449/171196 |
Resumo: | Although some studies in sheep have indicated leptospire colonization of the genital tract, further studies are needed to clarify the role of genital carriers in this species. Thus, this study aimed to evaluate the colonization of pathogenic leptospires in the genital and urinary tract of slaughtered sheep. Fifty-seven adult, female woolless sheep destined for slaughter were used. Renal (n = 57), bladder (n = 57), ovary (n = 34), uterine tube (n = 44), and uterus (n = 33) samples were collected for molecular detection of Leptospira sp. DNA, and blood samples (n = 57) for serological testing. The molecular testing was performed using polymerase chain reaction (PCR), and the serological testing was performed using microscopic serum agglutination test (MAT). Samples with amplifying DNA were subjected to genetic sequencing. In total, leptospiral DNA was found in the tissues of 44 (77.2%) sheep, whereas only nine animals were positive on both PCR and MAT; there was slight agreement between PCR and MAT techniques (k = 0.0268; p = 0.684). In 61 (54.9%) genital tract and in five (4.4%) urinary tract samples, the leptospiral DNA was detected, with significant difference (p < 0.001). The genes of one sample from the uterine tube and another from the bladder were sequenced and demonstrated 99% similarity to Leptospira interrogans. Anti-Leptospira antibodies were detected in 11 (19.3%) of the tested animals. The results reinforce the importance of the genital tract as an extra-renal site of colonization, suggesting the possibility of venereal transmission in sheep. |
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High frequency of genital carriers of Leptospira sp. in sheep slaughtered in the semi-arid region of northeastern BrazilGenital tractLeptospirosisSheepVenereal transmissionAlthough some studies in sheep have indicated leptospire colonization of the genital tract, further studies are needed to clarify the role of genital carriers in this species. Thus, this study aimed to evaluate the colonization of pathogenic leptospires in the genital and urinary tract of slaughtered sheep. Fifty-seven adult, female woolless sheep destined for slaughter were used. Renal (n = 57), bladder (n = 57), ovary (n = 34), uterine tube (n = 44), and uterus (n = 33) samples were collected for molecular detection of Leptospira sp. DNA, and blood samples (n = 57) for serological testing. The molecular testing was performed using polymerase chain reaction (PCR), and the serological testing was performed using microscopic serum agglutination test (MAT). Samples with amplifying DNA were subjected to genetic sequencing. In total, leptospiral DNA was found in the tissues of 44 (77.2%) sheep, whereas only nine animals were positive on both PCR and MAT; there was slight agreement between PCR and MAT techniques (k = 0.0268; p = 0.684). In 61 (54.9%) genital tract and in five (4.4%) urinary tract samples, the leptospiral DNA was detected, with significant difference (p < 0.001). The genes of one sample from the uterine tube and another from the bladder were sequenced and demonstrated 99% similarity to Leptospira interrogans. Anti-Leptospira antibodies were detected in 11 (19.3%) of the tested animals. The results reinforce the importance of the genital tract as an extra-renal site of colonization, suggesting the possibility of venereal transmission in sheep.Universidade Federal de Campina Grande (UFCG), Av. Universitária, s/n, Santa CecíliaUniversidade Estadual Paulista (Unesp) Instituto de BiotecnologiaUniversidade Estadual Paulista (Unesp) Instituto de BiotecnologiaUniversidade Federal de Campina Grande (UFCG)Universidade Estadual Paulista (Unesp)Silva, A. F.Farias, P. J.A.Silva, M. L.C.R.Araújo Júnior, J. P. [UNESP]Malossi, C. D. [UNESP]Ullmann, L. S. [UNESP]Costa, D. F.Higino, S. S.S.Azevedo, S. S.Alves, C. J.2018-12-11T16:54:21Z2018-12-11T16:54:21Z2018-07-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-5application/pdfhttp://dx.doi.org/10.1007/s11250-018-1657-9Tropical Animal Health and Production, p. 1-5.1573-74380049-4747http://hdl.handle.net/11449/17119610.1007/s11250-018-1657-92-s2.0-850496572302-s2.0-85049657230.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTropical Animal Health and Production0,5110,511info:eu-repo/semantics/openAccess2023-12-18T06:13:45Zoai:repositorio.unesp.br:11449/171196Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-18T06:13:45Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
High frequency of genital carriers of Leptospira sp. in sheep slaughtered in the semi-arid region of northeastern Brazil |
title |
High frequency of genital carriers of Leptospira sp. in sheep slaughtered in the semi-arid region of northeastern Brazil |
spellingShingle |
High frequency of genital carriers of Leptospira sp. in sheep slaughtered in the semi-arid region of northeastern Brazil Silva, A. F. Genital tract Leptospirosis Sheep Venereal transmission |
title_short |
High frequency of genital carriers of Leptospira sp. in sheep slaughtered in the semi-arid region of northeastern Brazil |
title_full |
High frequency of genital carriers of Leptospira sp. in sheep slaughtered in the semi-arid region of northeastern Brazil |
title_fullStr |
High frequency of genital carriers of Leptospira sp. in sheep slaughtered in the semi-arid region of northeastern Brazil |
title_full_unstemmed |
High frequency of genital carriers of Leptospira sp. in sheep slaughtered in the semi-arid region of northeastern Brazil |
title_sort |
High frequency of genital carriers of Leptospira sp. in sheep slaughtered in the semi-arid region of northeastern Brazil |
author |
Silva, A. F. |
author_facet |
Silva, A. F. Farias, P. J.A. Silva, M. L.C.R. Araújo Júnior, J. P. [UNESP] Malossi, C. D. [UNESP] Ullmann, L. S. [UNESP] Costa, D. F. Higino, S. S.S. Azevedo, S. S. Alves, C. J. |
author_role |
author |
author2 |
Farias, P. J.A. Silva, M. L.C.R. Araújo Júnior, J. P. [UNESP] Malossi, C. D. [UNESP] Ullmann, L. S. [UNESP] Costa, D. F. Higino, S. S.S. Azevedo, S. S. Alves, C. J. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Campina Grande (UFCG) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Silva, A. F. Farias, P. J.A. Silva, M. L.C.R. Araújo Júnior, J. P. [UNESP] Malossi, C. D. [UNESP] Ullmann, L. S. [UNESP] Costa, D. F. Higino, S. S.S. Azevedo, S. S. Alves, C. J. |
dc.subject.por.fl_str_mv |
Genital tract Leptospirosis Sheep Venereal transmission |
topic |
Genital tract Leptospirosis Sheep Venereal transmission |
description |
Although some studies in sheep have indicated leptospire colonization of the genital tract, further studies are needed to clarify the role of genital carriers in this species. Thus, this study aimed to evaluate the colonization of pathogenic leptospires in the genital and urinary tract of slaughtered sheep. Fifty-seven adult, female woolless sheep destined for slaughter were used. Renal (n = 57), bladder (n = 57), ovary (n = 34), uterine tube (n = 44), and uterus (n = 33) samples were collected for molecular detection of Leptospira sp. DNA, and blood samples (n = 57) for serological testing. The molecular testing was performed using polymerase chain reaction (PCR), and the serological testing was performed using microscopic serum agglutination test (MAT). Samples with amplifying DNA were subjected to genetic sequencing. In total, leptospiral DNA was found in the tissues of 44 (77.2%) sheep, whereas only nine animals were positive on both PCR and MAT; there was slight agreement between PCR and MAT techniques (k = 0.0268; p = 0.684). In 61 (54.9%) genital tract and in five (4.4%) urinary tract samples, the leptospiral DNA was detected, with significant difference (p < 0.001). The genes of one sample from the uterine tube and another from the bladder were sequenced and demonstrated 99% similarity to Leptospira interrogans. Anti-Leptospira antibodies were detected in 11 (19.3%) of the tested animals. The results reinforce the importance of the genital tract as an extra-renal site of colonization, suggesting the possibility of venereal transmission in sheep. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T16:54:21Z 2018-12-11T16:54:21Z 2018-07-09 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11250-018-1657-9 Tropical Animal Health and Production, p. 1-5. 1573-7438 0049-4747 http://hdl.handle.net/11449/171196 10.1007/s11250-018-1657-9 2-s2.0-85049657230 2-s2.0-85049657230.pdf |
url |
http://dx.doi.org/10.1007/s11250-018-1657-9 http://hdl.handle.net/11449/171196 |
identifier_str_mv |
Tropical Animal Health and Production, p. 1-5. 1573-7438 0049-4747 10.1007/s11250-018-1657-9 2-s2.0-85049657230 2-s2.0-85049657230.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Tropical Animal Health and Production 0,511 0,511 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-5 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965322814423040 |