Nanopartículas de prata associada ao trimetafosfato de sódio e fluoreto: síntese e avaliação do efeito antimicrobiano

• Autor(a) principal: Renesto, Ana Beatriz Repizo [UNESP]
• Data de Publicação: 2017
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/156625; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2018-06-12/000900563.pdf
• topic: Polifosfatos; Nanopartículas; Fluoretos; Microbiologia; Polyphosphates

Several researches have been conducted in order to develop new antimicrobial agents. Among these agents, silver nanoparticles (Ag-NP) demonstrated antimicrobial and antifungal activity against a broad spectrum of microorganisms. In addition, sodium trimetaphosphate (TMP) preserves the integrity and stability of the enamel and its association in appropriate proportions with fluoride (F) may provide a greater protective effect over conventional fluoride products. Thus, this study aimed to synthesize a compound containing silver nanoparticles (Ag-NP), sodium trimetaphosphate (TMP) and fluoride (F), characterize it and evaluate the antimicrobial action against planktonic cells of Candida albicans and Streptococcus mutans. For this, Ag-NP were synthesized by reducing silver nitrate at 1 (T1) or 10% (T10) in relation to TMP by a chemical route in the presence of F and TMP. The compound was characterized using scanning electron microscopy (SEM) and X-ray diffraction (XRD). It was also evaluated the silver ions concentration in the compound through an ion-specific electrode. The antimicrobial action in planktonic cells was evaluated by minimum inhibitory concentration (MIC) and minimum bactericidal or fungicidal concentration (MBC / MFC). SEM analyzes showed Ag-NP decorating the surface of the TMP particles and the effectiveness of the reaction was demonstrated by the reduction of silver ions greater than 90%. Both compounds presented antimicrobial activity against the evaluated microorganisms, however, Candida albicans was more susceptible. For Streptococcus mutans the MIC and MBC values were 5850 μg / ml and 11700 μg / ml respectively for compound T1, and 1463 μg / ml and 5850 μg / ml for compound T10. For Candida albicans the MIC and MFC values were 182.8 μg / ml and 2925 μg / ml respectively for compound T1, and 45.7 μg / ml and 731.2 μg / ml for compound T10. Thus, compounds synthesized by Ag-NP...