Análise estrutural e bioquímica da hidroxiapatita submetida ao tratamento com fluoreto e polifosfatos
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/123775 http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/20-05-2015/000828991.pdf |
Resumo: | This study evaluated the effect of sodium trimetaphosphate (TMP) and sodium hexametaphosphate (HMP) associated or no with fluoride (F) on the structure and dissolution of hydroxyapatite (HA). For this purpose, the study was divided into three subprojects. In Subprojects 1 and 2, synthetic HA powder (n=6) was treated with solutions containing TMP varying at 0-10% associated with 0, 100, 250, 500 (Subproject 1) and 0, 1100, 4500 and 9000 ppm F (Subproject 2) and submitted to a pH cycle. Afterwards, alkali- and acid soluble F, Ca and P were determined in/on HA, and P and F in the supernatants. Samples were analyzed by infrared spectroscopy (FTIR), X-ray diffraction (XDR) and energy-dispersive X-ray spectroscopy (EDX). In Subproject 3, HA discs were used to assess the dissolution rate of HA using a pHstat system. HA discs were treated with 1100 ppm F, 1% and 8% of HMP or TMP and 1100 ppm F associated with 1% and 8% of HMP or TMP, totaling 9 groups (n=8). The influence of a salivary pellicle on HA dissolution was also assessed. HA discs were kept in pooled human saliva before treatment with deionised water and 1100 ppm F associated with 1% and 8% of HMP or TMP, totaling 5 groups (n=8). The post-treatment dissolution rate was determined from three consecutive 30-min assays. Data were submitted to ANOVA, Student-Newman-Keuls' test and Pearson's correlation coefficient (Subprojects 1 and 2), and to ANOVA and Fisher and Holm- Sidak methods (Subproject 3) (α=0.05). Ca/P ratios values were higher for the fluoride solutions when compared with the no F and TMP groups and dependent of the F:TMP ratio used (p<0.05). The overall trend was that alkali-soluble F deposition was directly related to TMP concentrations in the treatment solutions, while acidsoluble incorporation was inversely related to TMP... |
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Análise estrutural e bioquímica da hidroxiapatita submetida ao tratamento com fluoreto e polifosfatosPolifosfatosFluoretosSalivaDurapatitaPolyphosphatesThis study evaluated the effect of sodium trimetaphosphate (TMP) and sodium hexametaphosphate (HMP) associated or no with fluoride (F) on the structure and dissolution of hydroxyapatite (HA). For this purpose, the study was divided into three subprojects. In Subprojects 1 and 2, synthetic HA powder (n=6) was treated with solutions containing TMP varying at 0-10% associated with 0, 100, 250, 500 (Subproject 1) and 0, 1100, 4500 and 9000 ppm F (Subproject 2) and submitted to a pH cycle. Afterwards, alkali- and acid soluble F, Ca and P were determined in/on HA, and P and F in the supernatants. Samples were analyzed by infrared spectroscopy (FTIR), X-ray diffraction (XDR) and energy-dispersive X-ray spectroscopy (EDX). In Subproject 3, HA discs were used to assess the dissolution rate of HA using a pHstat system. HA discs were treated with 1100 ppm F, 1% and 8% of HMP or TMP and 1100 ppm F associated with 1% and 8% of HMP or TMP, totaling 9 groups (n=8). The influence of a salivary pellicle on HA dissolution was also assessed. HA discs were kept in pooled human saliva before treatment with deionised water and 1100 ppm F associated with 1% and 8% of HMP or TMP, totaling 5 groups (n=8). The post-treatment dissolution rate was determined from three consecutive 30-min assays. Data were submitted to ANOVA, Student-Newman-Keuls' test and Pearson's correlation coefficient (Subprojects 1 and 2), and to ANOVA and Fisher and Holm- Sidak methods (Subproject 3) (α=0.05). Ca/P ratios values were higher for the fluoride solutions when compared with the no F and TMP groups and dependent of the F:TMP ratio used (p<0.05). The overall trend was that alkali-soluble F deposition was directly related to TMP concentrations in the treatment solutions, while acidsoluble incorporation was inversely related to TMP...Este estudo avaliou o efeito do trimetafosfato de sódio (TMP) e hexametafosfato de sódio (HMP) associados ou não ao fluoreto (F) na dissolução e estrutura da hidroxiapatita (HA). Para tanto, o estudo foi dividido em três subprojetos. Nos subprojetos 1 e 2, o pó de HA sintética (n=6) foi tratado com soluções contendo entre 0 e 10% de TMP associado a 0, 100, 250, 500 (Subprojeto 1) e a 0, 1100, 4500 e 9000 ppm F (Subprojeto 2), sendo submetido a um ciclo de pH. As concentrações de F alcáli- e ácido solúvel, cálcio (Ca) e fósforo (P) foram determinadas na HA, e as de P e F no sobrenadante. As amostras foram analisadas pela espectroscopia no infravermelho (FTIR), difração de Raios-X (DRX) e espectroscopia de energia dispersiva (EDX). No Subprojeto 3, discos de HA foram empregados para avaliar a taxa de dissolução da mesma utilizando o sistema pHstat. Estes foram tratados com soluções contendo 1100 ppm F, 1 e 8% de HMP ou TMP e 1100 ppm F associado com 1 e 8% de HMP ou TMP, totalizando 9 grupos (n=8). A influência da película salivar também foi avaliada. Discos de HA foram mantidos em saliva humana previamente ao tratamento com água deionizada e 1100 ppm F associado com 1 e 8% de HMP ou TMP, totalizando 5 grupos (n=8). A taxa de dissolução pós-tratamento foi determinada a partir de 3 medidas consecutivas a cada 30 min. Os dados foram submetidos a ANOVA, teste de Student-Newman-Keul's e coeficiente de correlação de Pearson (Subprojetos 1 e 2) e a ANOVA e testes de Fisher e Holm-Sidak (Subprojeto 3) (α=0,05). Valores de proporção Ca/P maiores foram observados para as soluções fluoretadas quando comparados à solução sem F e TMP, sendo os mesmos dependentes da proporção F:TMP (p<0,05). A deposição do F álcali-solúvel foi diretamente relacionada com as concentrações de TMP nas soluções...Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAPESP: 11/17234-9Universidade Estadual Paulista (Unesp)Delbem, Alberto Carlos Botazzo [UNESP]Pessan, Juliano Pelim [UNESP]Universidade Estadual Paulista (Unesp)Amaral, Jackeline Gallo do [UNESP]2015-06-17T19:33:55Z2015-06-17T19:33:55Z2014-09-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis112 f. : il. + 1 CD-ROMapplication/pdfAMARAL, Jackeline Gallo do. Análise estrutural e bioquímica da hidroxiapatita submetida ao tratamento com fluoreto e polifosfatos. 2014. 112 f. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Odontologia de Araçatuba, 2014.http://hdl.handle.net/11449/123775000828991http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/20-05-2015/000828991.pdf33004021073P5Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2023-12-26T06:17:11Zoai:repositorio.unesp.br:11449/123775Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-26T06:17:11Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Análise estrutural e bioquímica da hidroxiapatita submetida ao tratamento com fluoreto e polifosfatos |
title |
Análise estrutural e bioquímica da hidroxiapatita submetida ao tratamento com fluoreto e polifosfatos |
spellingShingle |
Análise estrutural e bioquímica da hidroxiapatita submetida ao tratamento com fluoreto e polifosfatos Amaral, Jackeline Gallo do [UNESP] Polifosfatos Fluoretos Saliva Durapatita Polyphosphates |
title_short |
Análise estrutural e bioquímica da hidroxiapatita submetida ao tratamento com fluoreto e polifosfatos |
title_full |
Análise estrutural e bioquímica da hidroxiapatita submetida ao tratamento com fluoreto e polifosfatos |
title_fullStr |
Análise estrutural e bioquímica da hidroxiapatita submetida ao tratamento com fluoreto e polifosfatos |
title_full_unstemmed |
Análise estrutural e bioquímica da hidroxiapatita submetida ao tratamento com fluoreto e polifosfatos |
title_sort |
Análise estrutural e bioquímica da hidroxiapatita submetida ao tratamento com fluoreto e polifosfatos |
author |
Amaral, Jackeline Gallo do [UNESP] |
author_facet |
Amaral, Jackeline Gallo do [UNESP] |
author_role |
author |
dc.contributor.none.fl_str_mv |
Delbem, Alberto Carlos Botazzo [UNESP] Pessan, Juliano Pelim [UNESP] Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Amaral, Jackeline Gallo do [UNESP] |
dc.subject.por.fl_str_mv |
Polifosfatos Fluoretos Saliva Durapatita Polyphosphates |
topic |
Polifosfatos Fluoretos Saliva Durapatita Polyphosphates |
description |
This study evaluated the effect of sodium trimetaphosphate (TMP) and sodium hexametaphosphate (HMP) associated or no with fluoride (F) on the structure and dissolution of hydroxyapatite (HA). For this purpose, the study was divided into three subprojects. In Subprojects 1 and 2, synthetic HA powder (n=6) was treated with solutions containing TMP varying at 0-10% associated with 0, 100, 250, 500 (Subproject 1) and 0, 1100, 4500 and 9000 ppm F (Subproject 2) and submitted to a pH cycle. Afterwards, alkali- and acid soluble F, Ca and P were determined in/on HA, and P and F in the supernatants. Samples were analyzed by infrared spectroscopy (FTIR), X-ray diffraction (XDR) and energy-dispersive X-ray spectroscopy (EDX). In Subproject 3, HA discs were used to assess the dissolution rate of HA using a pHstat system. HA discs were treated with 1100 ppm F, 1% and 8% of HMP or TMP and 1100 ppm F associated with 1% and 8% of HMP or TMP, totaling 9 groups (n=8). The influence of a salivary pellicle on HA dissolution was also assessed. HA discs were kept in pooled human saliva before treatment with deionised water and 1100 ppm F associated with 1% and 8% of HMP or TMP, totaling 5 groups (n=8). The post-treatment dissolution rate was determined from three consecutive 30-min assays. Data were submitted to ANOVA, Student-Newman-Keuls' test and Pearson's correlation coefficient (Subprojects 1 and 2), and to ANOVA and Fisher and Holm- Sidak methods (Subproject 3) (α=0.05). Ca/P ratios values were higher for the fluoride solutions when compared with the no F and TMP groups and dependent of the F:TMP ratio used (p<0.05). The overall trend was that alkali-soluble F deposition was directly related to TMP concentrations in the treatment solutions, while acidsoluble incorporation was inversely related to TMP... |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09-10 2015-06-17T19:33:55Z 2015-06-17T19:33:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
AMARAL, Jackeline Gallo do. Análise estrutural e bioquímica da hidroxiapatita submetida ao tratamento com fluoreto e polifosfatos. 2014. 112 f. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Odontologia de Araçatuba, 2014. http://hdl.handle.net/11449/123775 000828991 http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/20-05-2015/000828991.pdf 33004021073P5 |
identifier_str_mv |
AMARAL, Jackeline Gallo do. Análise estrutural e bioquímica da hidroxiapatita submetida ao tratamento com fluoreto e polifosfatos. 2014. 112 f. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Odontologia de Araçatuba, 2014. 000828991 33004021073P5 |
url |
http://hdl.handle.net/11449/123775 http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/20-05-2015/000828991.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
112 f. : il. + 1 CD-ROM application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.source.none.fl_str_mv |
Aleph reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803047121446764544 |