Beneficial effects of physical exercise on functional capacity and skeletal muscle oxidative stress in rats with aortic stenosis-induced heart failure

• Autor(a) principal: Gomes, Mariana Janini [UNESP]
• Data de Publicação: 2016
• Outros Autores: Martinez, Paula Felippe [UNESP], Campos, Dijon Henrique Salomé [UNESP], Pagan, Luana Urbano [UNESP], Bonomo, Camila [UNESP], Lima, Aline Regina Ruiz [UNESP], Damatto, Ricardo Luiz [UNESP], Cezar, Marcelo D. M. [UNESP], Damatto, Felipe Cezar [UNESP], Rosa, Camila Moreno [UNESP], Garcia, Camila Marchiolli [UNESP], Reyes, David Rafael Abreu [UNESP], Fernandes, Ana Angélica Henrique [UNESP], Fernandes, Denise Castro, Laurindo, Francisco Rafael, Okoshi, Katashi [UNESP], Okoshi, Marina Politi [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1155/2016/8695716; http://hdl.handle.net/11449/172545

Objective. We evaluated the influence of exercise on functional capacity, cardiac remodeling, and skeletal muscle oxidative stress, MAPK, and NF-B pathway in rats with aortic stenosis- (AS-) induced heart failure (HF). Methods and Results. Eighteen weeks after AS induction, rats were assigned into sedentary control (C-Sed), exercised control (C-Ex), sedentary AS (AS-Sed), and exercised AS (AS-Ex) groups. Exercise was performed on treadmill for eight weeks. Statistical analyses were performed with Goodman and ANOVA or Mann-Whitney. HF features frequency and mortality did not differ between AS groups. Exercise improved functional capacity, assessed by maximal exercise test on treadmill, without changing echocardiographic parameters. Soleus cross-sectional areas did not differ between groups. Lipid hydroperoxide concentration was higher in AS-Sed than C-Sed and AS-Ex. Activity of antioxidant enzymes superoxide dismutase and glutathione peroxidase was changed in AS-Sed and restored in AS-Ex. NADPH oxidase activity and gene expression of its subunits did not differ between AS groups. Total ROS generation was lower in AS-Ex than C-Ex. Exercise modulated MAPK in AS-Ex and did not change NF-B pathway proteins. Conclusion. Exercise improves functional capacity in rats with AS-induced HF regardless of echocardiographic parameter changes. In soleus, exercise reduces oxidative stress, preserves antioxidant enzyme activity, and modulates MAPK expression.