Exercise during transition from compensated left ventricular hypertrophy to heart failure in aortic stenosis rats

Detalhes bibliográficos
Autor(a) principal: Reyes, David R. A. [UNESP]
Data de Publicação: 2019
Outros Autores: Gomes, Mariana J. [UNESP], Rosa, Camila M. [UNESP], Pagan, Luana U. [UNESP], Zanati, Silmeia G. [UNESP], Damatto, Ricardo L. [UNESP], Rodrigues, Eder A. [UNESP], Carvalho, Robson F. [UNESP], Fernandes, Ana A. H. [UNESP], Martinez, Paula F., Lima, Aline R. R. [UNESP], Cezar, Marcelo D. M. [UNESP], Carvalho, Luiz E. F. M. [UNESP], Okoshi, Katashi [UNESP], Okoshi, Marina P. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1111/jcmm.14025
http://hdl.handle.net/11449/188380
Resumo: We evaluated the influence of aerobic exercise on cardiac remodelling during the transition from compensated left ventricular (LV) hypertrophy to clinical heart failure in aortic stenosis (AS) rats. Eighteen weeks after AS induction, rats were assigned into sedentary (AS) and exercised (AS-Ex) groups. Results were compared to Sham rats. Exercise was performed on treadmill for 8 weeks. Exercise improved functional capacity. Echocardiogram showed no differences between AS-Ex and AS groups. After exercise, fractional shortening and ejection fraction were lower in AS-Ex than Sham. Myocyte diameter and interstitial collagen fraction were higher in AS and AS-Ex than Sham; however, myocyte diameter was higher in AS-Ex than AS. Myocardial oxidative stress, evaluated by lipid hydroperoxide concentration, was higher in AS than Sham and was normalized by exercise. Gene expression of the NADPH oxidase subunits NOX2 and NOX4, which participate in ROS generation, did not differ between groups. Activity of the antioxidant enzyme superoxide dismutase was lower in AS and AS-Ex than Sham and glutathione peroxidase was lower in AS-Ex than Sham. Total and reduced myocardial glutathione, which is involved in cellular defence against oxidative stress, was lower in AS than Sham and total glutathione was higher in AS-Ex than AS. The MAPK JNK was higher in AS-Ex than Sham and AS groups. Phosphorylated P38 was lower in AS-Ex than AS. Despite improving functional capacity, aerobic exercise does not change LV function in AS rats. Exercise restores myocardial glutathione, reduces oxidative stress, impairs JNK signalling and further induces myocyte hypertrophy.
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spelling Exercise during transition from compensated left ventricular hypertrophy to heart failure in aortic stenosis ratscardiac remodellingechocardiogramMAPKoxidative stressphysical trainingratWe evaluated the influence of aerobic exercise on cardiac remodelling during the transition from compensated left ventricular (LV) hypertrophy to clinical heart failure in aortic stenosis (AS) rats. Eighteen weeks after AS induction, rats were assigned into sedentary (AS) and exercised (AS-Ex) groups. Results were compared to Sham rats. Exercise was performed on treadmill for 8 weeks. Exercise improved functional capacity. Echocardiogram showed no differences between AS-Ex and AS groups. After exercise, fractional shortening and ejection fraction were lower in AS-Ex than Sham. Myocyte diameter and interstitial collagen fraction were higher in AS and AS-Ex than Sham; however, myocyte diameter was higher in AS-Ex than AS. Myocardial oxidative stress, evaluated by lipid hydroperoxide concentration, was higher in AS than Sham and was normalized by exercise. Gene expression of the NADPH oxidase subunits NOX2 and NOX4, which participate in ROS generation, did not differ between groups. Activity of the antioxidant enzyme superoxide dismutase was lower in AS and AS-Ex than Sham and glutathione peroxidase was lower in AS-Ex than Sham. Total and reduced myocardial glutathione, which is involved in cellular defence against oxidative stress, was lower in AS than Sham and total glutathione was higher in AS-Ex than AS. The MAPK JNK was higher in AS-Ex than Sham and AS groups. Phosphorylated P38 was lower in AS-Ex than AS. Despite improving functional capacity, aerobic exercise does not change LV function in AS rats. Exercise restores myocardial glutathione, reduces oxidative stress, impairs JNK signalling and further induces myocyte hypertrophy.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual PaulistaBotucatu Medical School Internal Medicine Department Sao Paulo State University UNESPInstitute of Biosciences of Botucatu Sao Paulo State University UNESPSchool of Physical Therapy Federal University of Mato Grosso do SulBotucatu Medical School Internal Medicine Department Sao Paulo State University UNESPInstitute of Biosciences of Botucatu Sao Paulo State University UNESPFAPESP: 2014/21972-3Universidade Estadual Paulista (Unesp)Federal University of Mato Grosso do SulReyes, David R. A. [UNESP]Gomes, Mariana J. [UNESP]Rosa, Camila M. [UNESP]Pagan, Luana U. [UNESP]Zanati, Silmeia G. [UNESP]Damatto, Ricardo L. [UNESP]Rodrigues, Eder A. [UNESP]Carvalho, Robson F. [UNESP]Fernandes, Ana A. H. [UNESP]Martinez, Paula F.Lima, Aline R. R. [UNESP]Cezar, Marcelo D. M. [UNESP]Carvalho, Luiz E. F. M. [UNESP]Okoshi, Katashi [UNESP]Okoshi, Marina P. [UNESP]2019-10-06T16:06:12Z2019-10-06T16:06:12Z2019-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1235-1245http://dx.doi.org/10.1111/jcmm.14025Journal of Cellular and Molecular Medicine, v. 23, n. 2, p. 1235-1245, 2019.1582-1838http://hdl.handle.net/11449/18838010.1111/jcmm.140252-s2.0-85056781783Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Cellular and Molecular Medicineinfo:eu-repo/semantics/openAccess2024-08-14T17:22:26Zoai:repositorio.unesp.br:11449/188380Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:22:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Exercise during transition from compensated left ventricular hypertrophy to heart failure in aortic stenosis rats
title Exercise during transition from compensated left ventricular hypertrophy to heart failure in aortic stenosis rats
spellingShingle Exercise during transition from compensated left ventricular hypertrophy to heart failure in aortic stenosis rats
Reyes, David R. A. [UNESP]
cardiac remodelling
echocardiogram
MAPK
oxidative stress
physical training
rat
title_short Exercise during transition from compensated left ventricular hypertrophy to heart failure in aortic stenosis rats
title_full Exercise during transition from compensated left ventricular hypertrophy to heart failure in aortic stenosis rats
title_fullStr Exercise during transition from compensated left ventricular hypertrophy to heart failure in aortic stenosis rats
title_full_unstemmed Exercise during transition from compensated left ventricular hypertrophy to heart failure in aortic stenosis rats
title_sort Exercise during transition from compensated left ventricular hypertrophy to heart failure in aortic stenosis rats
author Reyes, David R. A. [UNESP]
author_facet Reyes, David R. A. [UNESP]
Gomes, Mariana J. [UNESP]
Rosa, Camila M. [UNESP]
Pagan, Luana U. [UNESP]
Zanati, Silmeia G. [UNESP]
Damatto, Ricardo L. [UNESP]
Rodrigues, Eder A. [UNESP]
Carvalho, Robson F. [UNESP]
Fernandes, Ana A. H. [UNESP]
Martinez, Paula F.
Lima, Aline R. R. [UNESP]
Cezar, Marcelo D. M. [UNESP]
Carvalho, Luiz E. F. M. [UNESP]
Okoshi, Katashi [UNESP]
Okoshi, Marina P. [UNESP]
author_role author
author2 Gomes, Mariana J. [UNESP]
Rosa, Camila M. [UNESP]
Pagan, Luana U. [UNESP]
Zanati, Silmeia G. [UNESP]
Damatto, Ricardo L. [UNESP]
Rodrigues, Eder A. [UNESP]
Carvalho, Robson F. [UNESP]
Fernandes, Ana A. H. [UNESP]
Martinez, Paula F.
Lima, Aline R. R. [UNESP]
Cezar, Marcelo D. M. [UNESP]
Carvalho, Luiz E. F. M. [UNESP]
Okoshi, Katashi [UNESP]
Okoshi, Marina P. [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Federal University of Mato Grosso do Sul
dc.contributor.author.fl_str_mv Reyes, David R. A. [UNESP]
Gomes, Mariana J. [UNESP]
Rosa, Camila M. [UNESP]
Pagan, Luana U. [UNESP]
Zanati, Silmeia G. [UNESP]
Damatto, Ricardo L. [UNESP]
Rodrigues, Eder A. [UNESP]
Carvalho, Robson F. [UNESP]
Fernandes, Ana A. H. [UNESP]
Martinez, Paula F.
Lima, Aline R. R. [UNESP]
Cezar, Marcelo D. M. [UNESP]
Carvalho, Luiz E. F. M. [UNESP]
Okoshi, Katashi [UNESP]
Okoshi, Marina P. [UNESP]
dc.subject.por.fl_str_mv cardiac remodelling
echocardiogram
MAPK
oxidative stress
physical training
rat
topic cardiac remodelling
echocardiogram
MAPK
oxidative stress
physical training
rat
description We evaluated the influence of aerobic exercise on cardiac remodelling during the transition from compensated left ventricular (LV) hypertrophy to clinical heart failure in aortic stenosis (AS) rats. Eighteen weeks after AS induction, rats were assigned into sedentary (AS) and exercised (AS-Ex) groups. Results were compared to Sham rats. Exercise was performed on treadmill for 8 weeks. Exercise improved functional capacity. Echocardiogram showed no differences between AS-Ex and AS groups. After exercise, fractional shortening and ejection fraction were lower in AS-Ex than Sham. Myocyte diameter and interstitial collagen fraction were higher in AS and AS-Ex than Sham; however, myocyte diameter was higher in AS-Ex than AS. Myocardial oxidative stress, evaluated by lipid hydroperoxide concentration, was higher in AS than Sham and was normalized by exercise. Gene expression of the NADPH oxidase subunits NOX2 and NOX4, which participate in ROS generation, did not differ between groups. Activity of the antioxidant enzyme superoxide dismutase was lower in AS and AS-Ex than Sham and glutathione peroxidase was lower in AS-Ex than Sham. Total and reduced myocardial glutathione, which is involved in cellular defence against oxidative stress, was lower in AS than Sham and total glutathione was higher in AS-Ex than AS. The MAPK JNK was higher in AS-Ex than Sham and AS groups. Phosphorylated P38 was lower in AS-Ex than AS. Despite improving functional capacity, aerobic exercise does not change LV function in AS rats. Exercise restores myocardial glutathione, reduces oxidative stress, impairs JNK signalling and further induces myocyte hypertrophy.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:06:12Z
2019-10-06T16:06:12Z
2019-02-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1111/jcmm.14025
Journal of Cellular and Molecular Medicine, v. 23, n. 2, p. 1235-1245, 2019.
1582-1838
http://hdl.handle.net/11449/188380
10.1111/jcmm.14025
2-s2.0-85056781783
url http://dx.doi.org/10.1111/jcmm.14025
http://hdl.handle.net/11449/188380
identifier_str_mv Journal of Cellular and Molecular Medicine, v. 23, n. 2, p. 1235-1245, 2019.
1582-1838
10.1111/jcmm.14025
2-s2.0-85056781783
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Cellular and Molecular Medicine
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1235-1245
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128118387376128