Exercise during transition from compensated left ventricular hypertrophy to heart failure in aortic stenosis rats
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/jcmm.14025 http://hdl.handle.net/11449/188380 |
Resumo: | We evaluated the influence of aerobic exercise on cardiac remodelling during the transition from compensated left ventricular (LV) hypertrophy to clinical heart failure in aortic stenosis (AS) rats. Eighteen weeks after AS induction, rats were assigned into sedentary (AS) and exercised (AS-Ex) groups. Results were compared to Sham rats. Exercise was performed on treadmill for 8 weeks. Exercise improved functional capacity. Echocardiogram showed no differences between AS-Ex and AS groups. After exercise, fractional shortening and ejection fraction were lower in AS-Ex than Sham. Myocyte diameter and interstitial collagen fraction were higher in AS and AS-Ex than Sham; however, myocyte diameter was higher in AS-Ex than AS. Myocardial oxidative stress, evaluated by lipid hydroperoxide concentration, was higher in AS than Sham and was normalized by exercise. Gene expression of the NADPH oxidase subunits NOX2 and NOX4, which participate in ROS generation, did not differ between groups. Activity of the antioxidant enzyme superoxide dismutase was lower in AS and AS-Ex than Sham and glutathione peroxidase was lower in AS-Ex than Sham. Total and reduced myocardial glutathione, which is involved in cellular defence against oxidative stress, was lower in AS than Sham and total glutathione was higher in AS-Ex than AS. The MAPK JNK was higher in AS-Ex than Sham and AS groups. Phosphorylated P38 was lower in AS-Ex than AS. Despite improving functional capacity, aerobic exercise does not change LV function in AS rats. Exercise restores myocardial glutathione, reduces oxidative stress, impairs JNK signalling and further induces myocyte hypertrophy. |
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Exercise during transition from compensated left ventricular hypertrophy to heart failure in aortic stenosis ratscardiac remodellingechocardiogramMAPKoxidative stressphysical trainingratWe evaluated the influence of aerobic exercise on cardiac remodelling during the transition from compensated left ventricular (LV) hypertrophy to clinical heart failure in aortic stenosis (AS) rats. Eighteen weeks after AS induction, rats were assigned into sedentary (AS) and exercised (AS-Ex) groups. Results were compared to Sham rats. Exercise was performed on treadmill for 8 weeks. Exercise improved functional capacity. Echocardiogram showed no differences between AS-Ex and AS groups. After exercise, fractional shortening and ejection fraction were lower in AS-Ex than Sham. Myocyte diameter and interstitial collagen fraction were higher in AS and AS-Ex than Sham; however, myocyte diameter was higher in AS-Ex than AS. Myocardial oxidative stress, evaluated by lipid hydroperoxide concentration, was higher in AS than Sham and was normalized by exercise. Gene expression of the NADPH oxidase subunits NOX2 and NOX4, which participate in ROS generation, did not differ between groups. Activity of the antioxidant enzyme superoxide dismutase was lower in AS and AS-Ex than Sham and glutathione peroxidase was lower in AS-Ex than Sham. Total and reduced myocardial glutathione, which is involved in cellular defence against oxidative stress, was lower in AS than Sham and total glutathione was higher in AS-Ex than AS. The MAPK JNK was higher in AS-Ex than Sham and AS groups. Phosphorylated P38 was lower in AS-Ex than AS. Despite improving functional capacity, aerobic exercise does not change LV function in AS rats. Exercise restores myocardial glutathione, reduces oxidative stress, impairs JNK signalling and further induces myocyte hypertrophy.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual PaulistaBotucatu Medical School Internal Medicine Department Sao Paulo State University UNESPInstitute of Biosciences of Botucatu Sao Paulo State University UNESPSchool of Physical Therapy Federal University of Mato Grosso do SulBotucatu Medical School Internal Medicine Department Sao Paulo State University UNESPInstitute of Biosciences of Botucatu Sao Paulo State University UNESPFAPESP: 2014/21972-3Universidade Estadual Paulista (Unesp)Federal University of Mato Grosso do SulReyes, David R. A. [UNESP]Gomes, Mariana J. [UNESP]Rosa, Camila M. [UNESP]Pagan, Luana U. [UNESP]Zanati, Silmeia G. [UNESP]Damatto, Ricardo L. [UNESP]Rodrigues, Eder A. [UNESP]Carvalho, Robson F. [UNESP]Fernandes, Ana A. H. [UNESP]Martinez, Paula F.Lima, Aline R. R. [UNESP]Cezar, Marcelo D. M. [UNESP]Carvalho, Luiz E. F. M. [UNESP]Okoshi, Katashi [UNESP]Okoshi, Marina P. [UNESP]2019-10-06T16:06:12Z2019-10-06T16:06:12Z2019-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1235-1245http://dx.doi.org/10.1111/jcmm.14025Journal of Cellular and Molecular Medicine, v. 23, n. 2, p. 1235-1245, 2019.1582-1838http://hdl.handle.net/11449/18838010.1111/jcmm.140252-s2.0-85056781783Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Cellular and Molecular Medicineinfo:eu-repo/semantics/openAccess2024-08-14T17:22:26Zoai:repositorio.unesp.br:11449/188380Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:22:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Exercise during transition from compensated left ventricular hypertrophy to heart failure in aortic stenosis rats |
title |
Exercise during transition from compensated left ventricular hypertrophy to heart failure in aortic stenosis rats |
spellingShingle |
Exercise during transition from compensated left ventricular hypertrophy to heart failure in aortic stenosis rats Reyes, David R. A. [UNESP] cardiac remodelling echocardiogram MAPK oxidative stress physical training rat |
title_short |
Exercise during transition from compensated left ventricular hypertrophy to heart failure in aortic stenosis rats |
title_full |
Exercise during transition from compensated left ventricular hypertrophy to heart failure in aortic stenosis rats |
title_fullStr |
Exercise during transition from compensated left ventricular hypertrophy to heart failure in aortic stenosis rats |
title_full_unstemmed |
Exercise during transition from compensated left ventricular hypertrophy to heart failure in aortic stenosis rats |
title_sort |
Exercise during transition from compensated left ventricular hypertrophy to heart failure in aortic stenosis rats |
author |
Reyes, David R. A. [UNESP] |
author_facet |
Reyes, David R. A. [UNESP] Gomes, Mariana J. [UNESP] Rosa, Camila M. [UNESP] Pagan, Luana U. [UNESP] Zanati, Silmeia G. [UNESP] Damatto, Ricardo L. [UNESP] Rodrigues, Eder A. [UNESP] Carvalho, Robson F. [UNESP] Fernandes, Ana A. H. [UNESP] Martinez, Paula F. Lima, Aline R. R. [UNESP] Cezar, Marcelo D. M. [UNESP] Carvalho, Luiz E. F. M. [UNESP] Okoshi, Katashi [UNESP] Okoshi, Marina P. [UNESP] |
author_role |
author |
author2 |
Gomes, Mariana J. [UNESP] Rosa, Camila M. [UNESP] Pagan, Luana U. [UNESP] Zanati, Silmeia G. [UNESP] Damatto, Ricardo L. [UNESP] Rodrigues, Eder A. [UNESP] Carvalho, Robson F. [UNESP] Fernandes, Ana A. H. [UNESP] Martinez, Paula F. Lima, Aline R. R. [UNESP] Cezar, Marcelo D. M. [UNESP] Carvalho, Luiz E. F. M. [UNESP] Okoshi, Katashi [UNESP] Okoshi, Marina P. [UNESP] |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Federal University of Mato Grosso do Sul |
dc.contributor.author.fl_str_mv |
Reyes, David R. A. [UNESP] Gomes, Mariana J. [UNESP] Rosa, Camila M. [UNESP] Pagan, Luana U. [UNESP] Zanati, Silmeia G. [UNESP] Damatto, Ricardo L. [UNESP] Rodrigues, Eder A. [UNESP] Carvalho, Robson F. [UNESP] Fernandes, Ana A. H. [UNESP] Martinez, Paula F. Lima, Aline R. R. [UNESP] Cezar, Marcelo D. M. [UNESP] Carvalho, Luiz E. F. M. [UNESP] Okoshi, Katashi [UNESP] Okoshi, Marina P. [UNESP] |
dc.subject.por.fl_str_mv |
cardiac remodelling echocardiogram MAPK oxidative stress physical training rat |
topic |
cardiac remodelling echocardiogram MAPK oxidative stress physical training rat |
description |
We evaluated the influence of aerobic exercise on cardiac remodelling during the transition from compensated left ventricular (LV) hypertrophy to clinical heart failure in aortic stenosis (AS) rats. Eighteen weeks after AS induction, rats were assigned into sedentary (AS) and exercised (AS-Ex) groups. Results were compared to Sham rats. Exercise was performed on treadmill for 8 weeks. Exercise improved functional capacity. Echocardiogram showed no differences between AS-Ex and AS groups. After exercise, fractional shortening and ejection fraction were lower in AS-Ex than Sham. Myocyte diameter and interstitial collagen fraction were higher in AS and AS-Ex than Sham; however, myocyte diameter was higher in AS-Ex than AS. Myocardial oxidative stress, evaluated by lipid hydroperoxide concentration, was higher in AS than Sham and was normalized by exercise. Gene expression of the NADPH oxidase subunits NOX2 and NOX4, which participate in ROS generation, did not differ between groups. Activity of the antioxidant enzyme superoxide dismutase was lower in AS and AS-Ex than Sham and glutathione peroxidase was lower in AS-Ex than Sham. Total and reduced myocardial glutathione, which is involved in cellular defence against oxidative stress, was lower in AS than Sham and total glutathione was higher in AS-Ex than AS. The MAPK JNK was higher in AS-Ex than Sham and AS groups. Phosphorylated P38 was lower in AS-Ex than AS. Despite improving functional capacity, aerobic exercise does not change LV function in AS rats. Exercise restores myocardial glutathione, reduces oxidative stress, impairs JNK signalling and further induces myocyte hypertrophy. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:06:12Z 2019-10-06T16:06:12Z 2019-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/jcmm.14025 Journal of Cellular and Molecular Medicine, v. 23, n. 2, p. 1235-1245, 2019. 1582-1838 http://hdl.handle.net/11449/188380 10.1111/jcmm.14025 2-s2.0-85056781783 |
url |
http://dx.doi.org/10.1111/jcmm.14025 http://hdl.handle.net/11449/188380 |
identifier_str_mv |
Journal of Cellular and Molecular Medicine, v. 23, n. 2, p. 1235-1245, 2019. 1582-1838 10.1111/jcmm.14025 2-s2.0-85056781783 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Cellular and Molecular Medicine |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1235-1245 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128118387376128 |