Environmental isolation, biochemical identification, and antifungal drug susceptibility of Cryptococcus species

Detalhes bibliográficos
Autor(a) principal: Teodoro, Valter Luis Iost
Data de Publicação: 2013
Outros Autores: Gullo, Fernanda Patricia, Sardi, Janaina De Cassia Orlandi, Torres, Edson Maria, Fusco-almeida, Ana Marisa, Mendes-Giannini, Maria José Soares [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/0037-8682-0025-2013
http://hdl.handle.net/11449/109431
Resumo: Introduction The incidence of opportunistic fungal infections has increased in recent years and is considered an important public health problem. Among systemic and opportunistic mycoses, cryptococcosis is distinguished by its clinical importance due to the increased risk of infection in individuals infected by human immunodeficiency virus. Methods To determine the occurrence of pathogenic Cryptococcus in pigeon excrement in the City of Araraquara, samples were collected from nine environments, including state and municipal schools, abandoned buildings, parks, and a hospital. The isolates were identified using classical tests, and susceptibility testing for the antifungal drugs (fluconazole, itraconazole, voriconazole, and amphotericin B) independently was also performed. After collection, the excrement samples were plated on Niger agar and incubated at room temperature. Results A total of 87 bird dropping samples were collected, and 66.6% were positive for the genus Cryptococcus. The following species were identified: Cryptococcus neoformans (17.2%), Cryptococcus gattii (5.2%), Cryptococcus ater (3.5%), Cryptococcus laurentti (1.7%), and Cryptococcus luteolus (1.7%). A total of 70.7% of the isolates were not identified to the species level and are referred to as Cryptococcus spp. throughout the manuscript. Conclusions Although none of the isolates demonstrated resistance to antifungal drugs, the identification of infested areas, the proper control of birds, and the disinfection of these environments are essential for the epidemiological control of cryptococcosis.
id UNSP_486fadacc7f24a0d59f0998a4b235783
oai_identifier_str oai:repositorio.unesp.br:11449/109431
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Environmental isolation, biochemical identification, and antifungal drug susceptibility of Cryptococcus speciesCryptococcus sppEpidemiologyAntifungal DrugsEnvironmentIntroduction The incidence of opportunistic fungal infections has increased in recent years and is considered an important public health problem. Among systemic and opportunistic mycoses, cryptococcosis is distinguished by its clinical importance due to the increased risk of infection in individuals infected by human immunodeficiency virus. Methods To determine the occurrence of pathogenic Cryptococcus in pigeon excrement in the City of Araraquara, samples were collected from nine environments, including state and municipal schools, abandoned buildings, parks, and a hospital. The isolates were identified using classical tests, and susceptibility testing for the antifungal drugs (fluconazole, itraconazole, voriconazole, and amphotericin B) independently was also performed. After collection, the excrement samples were plated on Niger agar and incubated at room temperature. Results A total of 87 bird dropping samples were collected, and 66.6% were positive for the genus Cryptococcus. The following species were identified: Cryptococcus neoformans (17.2%), Cryptococcus gattii (5.2%), Cryptococcus ater (3.5%), Cryptococcus laurentti (1.7%), and Cryptococcus luteolus (1.7%). A total of 70.7% of the isolates were not identified to the species level and are referred to as Cryptococcus spp. throughout the manuscript. Conclusions Although none of the isolates demonstrated resistance to antifungal drugs, the identification of infested areas, the proper control of birds, and the disinfection of these environments are essential for the epidemiological control of cryptococcosis.Universidade Estadual Paulista (UNESP) Faculdade de Ciencias Farmaceuticas Laboratorio de Micologia ClinicaPrefeitura Municipal de Araraquara Departamento do Meio AmbienteUniversidade Estadual Paulista (UNESP) Faculdade de Ciencias Farmaceuticas Laboratorio de Micologia ClinicaSociedade Brasileira de Medicina Tropical - SBMTUniversidade Estadual Paulista (Unesp)Prefeitura Municipal de Araraquara Departamento do Meio AmbienteTeodoro, Valter Luis IostGullo, Fernanda PatriciaSardi, Janaina De Cassia OrlandiTorres, Edson MariaFusco-almeida, Ana MarisaMendes-Giannini, Maria José Soares [UNESP]2014-09-30T18:18:19Z2014-09-30T18:18:19Z2013-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article759-764application/pdfhttp://dx.doi.org/10.1590/0037-8682-0025-2013Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 46, n. 6, p. 759-764, 2013.0037-8682http://hdl.handle.net/11449/10943110.1590/0037-8682-0025-2013S0037-86822013000600759WOS:000330539200015S0037-86822013000600759.pdf0000-0002-8059-0826SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista da Sociedade Brasileira de Medicina Tropical1.3580,658info:eu-repo/semantics/openAccess2024-06-21T15:18:34Zoai:repositorio.unesp.br:11449/109431Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:17:19.878624Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Environmental isolation, biochemical identification, and antifungal drug susceptibility of Cryptococcus species
title Environmental isolation, biochemical identification, and antifungal drug susceptibility of Cryptococcus species
spellingShingle Environmental isolation, biochemical identification, and antifungal drug susceptibility of Cryptococcus species
Teodoro, Valter Luis Iost
Cryptococcus spp
Epidemiology
Antifungal Drugs
Environment
title_short Environmental isolation, biochemical identification, and antifungal drug susceptibility of Cryptococcus species
title_full Environmental isolation, biochemical identification, and antifungal drug susceptibility of Cryptococcus species
title_fullStr Environmental isolation, biochemical identification, and antifungal drug susceptibility of Cryptococcus species
title_full_unstemmed Environmental isolation, biochemical identification, and antifungal drug susceptibility of Cryptococcus species
title_sort Environmental isolation, biochemical identification, and antifungal drug susceptibility of Cryptococcus species
author Teodoro, Valter Luis Iost
author_facet Teodoro, Valter Luis Iost
Gullo, Fernanda Patricia
Sardi, Janaina De Cassia Orlandi
Torres, Edson Maria
Fusco-almeida, Ana Marisa
Mendes-Giannini, Maria José Soares [UNESP]
author_role author
author2 Gullo, Fernanda Patricia
Sardi, Janaina De Cassia Orlandi
Torres, Edson Maria
Fusco-almeida, Ana Marisa
Mendes-Giannini, Maria José Soares [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Prefeitura Municipal de Araraquara Departamento do Meio Ambiente
dc.contributor.author.fl_str_mv Teodoro, Valter Luis Iost
Gullo, Fernanda Patricia
Sardi, Janaina De Cassia Orlandi
Torres, Edson Maria
Fusco-almeida, Ana Marisa
Mendes-Giannini, Maria José Soares [UNESP]
dc.subject.por.fl_str_mv Cryptococcus spp
Epidemiology
Antifungal Drugs
Environment
topic Cryptococcus spp
Epidemiology
Antifungal Drugs
Environment
description Introduction The incidence of opportunistic fungal infections has increased in recent years and is considered an important public health problem. Among systemic and opportunistic mycoses, cryptococcosis is distinguished by its clinical importance due to the increased risk of infection in individuals infected by human immunodeficiency virus. Methods To determine the occurrence of pathogenic Cryptococcus in pigeon excrement in the City of Araraquara, samples were collected from nine environments, including state and municipal schools, abandoned buildings, parks, and a hospital. The isolates were identified using classical tests, and susceptibility testing for the antifungal drugs (fluconazole, itraconazole, voriconazole, and amphotericin B) independently was also performed. After collection, the excrement samples were plated on Niger agar and incubated at room temperature. Results A total of 87 bird dropping samples were collected, and 66.6% were positive for the genus Cryptococcus. The following species were identified: Cryptococcus neoformans (17.2%), Cryptococcus gattii (5.2%), Cryptococcus ater (3.5%), Cryptococcus laurentti (1.7%), and Cryptococcus luteolus (1.7%). A total of 70.7% of the isolates were not identified to the species level and are referred to as Cryptococcus spp. throughout the manuscript. Conclusions Although none of the isolates demonstrated resistance to antifungal drugs, the identification of infested areas, the proper control of birds, and the disinfection of these environments are essential for the epidemiological control of cryptococcosis.
publishDate 2013
dc.date.none.fl_str_mv 2013-12-01
2014-09-30T18:18:19Z
2014-09-30T18:18:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/0037-8682-0025-2013
Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 46, n. 6, p. 759-764, 2013.
0037-8682
http://hdl.handle.net/11449/109431
10.1590/0037-8682-0025-2013
S0037-86822013000600759
WOS:000330539200015
S0037-86822013000600759.pdf
0000-0002-8059-0826
url http://dx.doi.org/10.1590/0037-8682-0025-2013
http://hdl.handle.net/11449/109431
identifier_str_mv Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 46, n. 6, p. 759-764, 2013.
0037-8682
10.1590/0037-8682-0025-2013
S0037-86822013000600759
WOS:000330539200015
S0037-86822013000600759.pdf
0000-0002-8059-0826
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical
1.358
0,658
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 759-764
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128629495824384