3D printed geopolymer: An efficient support for immobilization of Candida rugosa lipase
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.cej.2021.128843 http://hdl.handle.net/11449/207257 |
Resumo: | Lattice-shaped geopolymers were successfully prepared by Direct Ink Writing to act as carriers for the immobilization of Candida rugosa lipase (CRL). The supported biocatalyst was evaluated in the hydrolysis of waste cooking oil (WCO), a preliminary step for the production of biodiesel. The lattice samples presented total and open porosities of 57.2 vol% and 56.4 vol% respectively, bulk density of 0.924 ± 0.059 g/cm3, and true density of struts of 2.157 ± 0.014 g/cm3. The permeability coefficients (k1 = (9.05 ± 0.41) × 10−9 m2 and k2 = (3.64 ± 0.26) × 10−4 m) were consistently higher than those of typical enzymatic carriers. The geopolymer surface was successfully modified to allow the immobilization process by covalent bonding of CRL. The hydrolytic activity reached 847.7 ± 9.7 U/g. A free fatty acids content of 75 wt% was achieved from the hydrolysis of WCO, proving the efficiency of immobilization and the suitability of lattice-shaped geopolymers as support for biocatalysts. |
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Repositório Institucional da UNESP |
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2946 |
spelling |
3D printed geopolymer: An efficient support for immobilization of Candida rugosa lipaseDirect ink writingGeopolymerImmobilizationLattice biocatalyst carrierLipaseLattice-shaped geopolymers were successfully prepared by Direct Ink Writing to act as carriers for the immobilization of Candida rugosa lipase (CRL). The supported biocatalyst was evaluated in the hydrolysis of waste cooking oil (WCO), a preliminary step for the production of biodiesel. The lattice samples presented total and open porosities of 57.2 vol% and 56.4 vol% respectively, bulk density of 0.924 ± 0.059 g/cm3, and true density of struts of 2.157 ± 0.014 g/cm3. The permeability coefficients (k1 = (9.05 ± 0.41) × 10−9 m2 and k2 = (3.64 ± 0.26) × 10−4 m) were consistently higher than those of typical enzymatic carriers. The geopolymer surface was successfully modified to allow the immobilization process by covalent bonding of CRL. The hydrolytic activity reached 847.7 ± 9.7 U/g. A free fatty acids content of 75 wt% was achieved from the hydrolysis of WCO, proving the efficiency of immobilization and the suitability of lattice-shaped geopolymers as support for biocatalysts.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)UNESP – São Paulo State University Institute of Chemistry Organic Chemistry Department – Center for Monitoring and Research of the Quality of Fuels Biofuels Crude Oil and Derivatives – CEMPEQCUniversity of Padova Department of Industrial EngineeringUNAERP – University of Ribeirão Preto Course of Chemical EngineeringPennsylvania State University Department of Materials Science and EngineeringUNESP – São Paulo State University School of Pharmaceutical Science (FCF) Department of Bioprocess Engineering and BiotechnologyMato Grosso Federal Institute of Education Science and Technology – Campus CuiabáUNESP – São Paulo State University Institute of Chemistry Organic Chemistry Department – Center for Monitoring and Research of the Quality of Fuels Biofuels Crude Oil and Derivatives – CEMPEQCUNESP – São Paulo State University School of Pharmaceutical Science (FCF) Department of Bioprocess Engineering and BiotechnologyUniversidade Estadual Paulista (Unesp)University of PadovaCourse of Chemical EngineeringPennsylvania State UniversityScience and Technology – Campus Cuiabádos Santos, Letícia Karen [UNESP]Botti, Renata FussInnocentini, Murilo Daniel de MelloMarques, Rodrigo Fernando Costa [UNESP]Colombo, Paolode Paula, Ariela Veloso [UNESP]Flumignan, Danilo Luiz [UNESP]2021-06-25T10:52:06Z2021-06-25T10:52:06Z2021-06-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.cej.2021.128843Chemical Engineering Journal, v. 414.1385-8947http://hdl.handle.net/11449/20725710.1016/j.cej.2021.1288432-s2.0-85100601676Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemical Engineering Journalinfo:eu-repo/semantics/openAccess2021-10-23T16:43:10Zoai:repositorio.unesp.br:11449/207257Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:08:05.715030Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
3D printed geopolymer: An efficient support for immobilization of Candida rugosa lipase |
title |
3D printed geopolymer: An efficient support for immobilization of Candida rugosa lipase |
spellingShingle |
3D printed geopolymer: An efficient support for immobilization of Candida rugosa lipase dos Santos, Letícia Karen [UNESP] Direct ink writing Geopolymer Immobilization Lattice biocatalyst carrier Lipase |
title_short |
3D printed geopolymer: An efficient support for immobilization of Candida rugosa lipase |
title_full |
3D printed geopolymer: An efficient support for immobilization of Candida rugosa lipase |
title_fullStr |
3D printed geopolymer: An efficient support for immobilization of Candida rugosa lipase |
title_full_unstemmed |
3D printed geopolymer: An efficient support for immobilization of Candida rugosa lipase |
title_sort |
3D printed geopolymer: An efficient support for immobilization of Candida rugosa lipase |
author |
dos Santos, Letícia Karen [UNESP] |
author_facet |
dos Santos, Letícia Karen [UNESP] Botti, Renata Fuss Innocentini, Murilo Daniel de Mello Marques, Rodrigo Fernando Costa [UNESP] Colombo, Paolo de Paula, Ariela Veloso [UNESP] Flumignan, Danilo Luiz [UNESP] |
author_role |
author |
author2 |
Botti, Renata Fuss Innocentini, Murilo Daniel de Mello Marques, Rodrigo Fernando Costa [UNESP] Colombo, Paolo de Paula, Ariela Veloso [UNESP] Flumignan, Danilo Luiz [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Padova Course of Chemical Engineering Pennsylvania State University Science and Technology – Campus Cuiabá |
dc.contributor.author.fl_str_mv |
dos Santos, Letícia Karen [UNESP] Botti, Renata Fuss Innocentini, Murilo Daniel de Mello Marques, Rodrigo Fernando Costa [UNESP] Colombo, Paolo de Paula, Ariela Veloso [UNESP] Flumignan, Danilo Luiz [UNESP] |
dc.subject.por.fl_str_mv |
Direct ink writing Geopolymer Immobilization Lattice biocatalyst carrier Lipase |
topic |
Direct ink writing Geopolymer Immobilization Lattice biocatalyst carrier Lipase |
description |
Lattice-shaped geopolymers were successfully prepared by Direct Ink Writing to act as carriers for the immobilization of Candida rugosa lipase (CRL). The supported biocatalyst was evaluated in the hydrolysis of waste cooking oil (WCO), a preliminary step for the production of biodiesel. The lattice samples presented total and open porosities of 57.2 vol% and 56.4 vol% respectively, bulk density of 0.924 ± 0.059 g/cm3, and true density of struts of 2.157 ± 0.014 g/cm3. The permeability coefficients (k1 = (9.05 ± 0.41) × 10−9 m2 and k2 = (3.64 ± 0.26) × 10−4 m) were consistently higher than those of typical enzymatic carriers. The geopolymer surface was successfully modified to allow the immobilization process by covalent bonding of CRL. The hydrolytic activity reached 847.7 ± 9.7 U/g. A free fatty acids content of 75 wt% was achieved from the hydrolysis of WCO, proving the efficiency of immobilization and the suitability of lattice-shaped geopolymers as support for biocatalysts. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:52:06Z 2021-06-25T10:52:06Z 2021-06-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.cej.2021.128843 Chemical Engineering Journal, v. 414. 1385-8947 http://hdl.handle.net/11449/207257 10.1016/j.cej.2021.128843 2-s2.0-85100601676 |
url |
http://dx.doi.org/10.1016/j.cej.2021.128843 http://hdl.handle.net/11449/207257 |
identifier_str_mv |
Chemical Engineering Journal, v. 414. 1385-8947 10.1016/j.cej.2021.128843 2-s2.0-85100601676 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Chemical Engineering Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129492989771776 |