Viabilidade do sêmen congelado obtido do epidídimo de touros post-mortem

• Autor(a) principal: Barbosa, Luciano Munita
• Data de Publicação: 2012
• Outros Autores: Kanazawa, Mábilis Yumi, Peres, Anelise Ribeiro [UNESP], Souza, Fabiana Ferreira de
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.4322/rbcv.2014.097; http://hdl.handle.net/11449/141012
• topic: Criopreserved; Ejaculated; Bovine; Sperm; Epididymal; Criopreservação; Ejaculado; Bovino; Espermatozoide; Epididimal

The objective of this study was to evaluate the effects of freezing on the integrity and functionality of bovine epididymal spermatozoa collected postmortem. Ten pairs of testicle epididymis of bulls from a commercial slaughterhouse were used. On the laboratory, the testicle was separated and the semen was collected from the tail of epididymis on a petri dish heated containing a medium (Botubov ®, Botupharma, Botucatu, SP). Then, the semen was evaluated to motility, vigor, concentration, morphology, DNA integrity, mitochondrial activity, viability, membrane integrity and functionality. The semen was diluted in commercial extender (Botubov ®, Botupharma, Botucatu, SP) at a concentration of 100x106 sperm/mL. Thereafter, the samples were packaged in straws 0.5 mL, chilled at 4° C, for 4 hours, frozen in polystyrene box, at 6 cm from level of liquid nitrogen, during 20 minutes and stored in cryogenic container. The samples were thawed at 37°C for 30 seconds. After thawing, the semen was reevaluated to the same parameters, except sperm concentration and volume. Comparison of results between fresh and frozen semen showed means were increased (p<0.05) fresh semen motility (74,0 ± 7,0% versus 30,0 ± 8,2%), vigor (3,6 ± 0,7 versus 1,3 ± 0,5), membrane integrity (fluorescence, 95,6 ± 3,1% versus 75,7 ± 5,9%) and index of mitochondrial activity (99,3 ± 6,6 versus 40,0 ± 6,3), respectively. In conclusion, the viability of frozen semen obtained from the epididymis of bulls is similar than ejaculated semen, making the collection technique commercially applicable.