Viabilidade do sêmen congelado obtido do epidídimo de touros post-mortem

Detalhes bibliográficos
Autor(a) principal: Barbosa, Luciano Munita
Data de Publicação: 2012
Outros Autores: Kanazawa, Mábilis Yumi, Peres, Anelise Ribeiro [UNESP], Souza, Fabiana Ferreira de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.4322/rbcv.2014.097
http://hdl.handle.net/11449/141012
Resumo: The objective of this study was to evaluate the effects of freezing on the integrity and functionality of bovine epididymal spermatozoa collected postmortem. Ten pairs of testicle epididymis of bulls from a commercial slaughterhouse were used. On the laboratory, the testicle was separated and the semen was collected from the tail of epididymis on a petri dish heated containing a medium (Botubov ®, Botupharma, Botucatu, SP). Then, the semen was evaluated to motility, vigor, concentration, morphology, DNA integrity, mitochondrial activity, viability, membrane integrity and functionality. The semen was diluted in commercial extender (Botubov ®, Botupharma, Botucatu, SP) at a concentration of 100x106 sperm/mL. Thereafter, the samples were packaged in straws 0.5 mL, chilled at 4° C, for 4 hours, frozen in polystyrene box, at 6 cm from level of liquid nitrogen, during 20 minutes and stored in cryogenic container. The samples were thawed at 37°C for 30 seconds. After thawing, the semen was reevaluated to the same parameters, except sperm concentration and volume. Comparison of results between fresh and frozen semen showed means were increased (p<0.05) fresh semen motility (74,0 ± 7,0% versus 30,0 ± 8,2%), vigor (3,6 ± 0,7 versus 1,3 ± 0,5), membrane integrity (fluorescence, 95,6 ± 3,1% versus 75,7 ± 5,9%) and index of mitochondrial activity (99,3 ± 6,6 versus 40,0 ± 6,3), respectively. In conclusion, the viability of frozen semen obtained from the epididymis of bulls is similar than ejaculated semen, making the collection technique commercially applicable.
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spelling Viabilidade do sêmen congelado obtido do epidídimo de touros post-mortemViability of frozen semen obtained from epididymis of bulls post-mortemCriopreservedEjaculatedBovineSpermEpididymalCriopreservaçãoEjaculadoBovinoEspermatozoideEpididimalThe objective of this study was to evaluate the effects of freezing on the integrity and functionality of bovine epididymal spermatozoa collected postmortem. Ten pairs of testicle epididymis of bulls from a commercial slaughterhouse were used. On the laboratory, the testicle was separated and the semen was collected from the tail of epididymis on a petri dish heated containing a medium (Botubov ®, Botupharma, Botucatu, SP). Then, the semen was evaluated to motility, vigor, concentration, morphology, DNA integrity, mitochondrial activity, viability, membrane integrity and functionality. The semen was diluted in commercial extender (Botubov ®, Botupharma, Botucatu, SP) at a concentration of 100x106 sperm/mL. Thereafter, the samples were packaged in straws 0.5 mL, chilled at 4° C, for 4 hours, frozen in polystyrene box, at 6 cm from level of liquid nitrogen, during 20 minutes and stored in cryogenic container. The samples were thawed at 37°C for 30 seconds. After thawing, the semen was reevaluated to the same parameters, except sperm concentration and volume. Comparison of results between fresh and frozen semen showed means were increased (p<0.05) fresh semen motility (74,0 ± 7,0% versus 30,0 ± 8,2%), vigor (3,6 ± 0,7 versus 1,3 ± 0,5), membrane integrity (fluorescence, 95,6 ± 3,1% versus 75,7 ± 5,9%) and index of mitochondrial activity (99,3 ± 6,6 versus 40,0 ± 6,3), respectively. In conclusion, the viability of frozen semen obtained from the epididymis of bulls is similar than ejaculated semen, making the collection technique commercially applicable.O objetivo deste estudo foi avaliar os efeitos da congelação sobre a integridade e funcionalidade de espermatozoides colhidos do epidídimo de touros post-mortem. Foram utilizados 10 pares de testículos de touros provenientes de um abatedouro comercial. No laboratório, o sêmen foi colhido da cauda do epidídimo sobre uma placa de Petri aquecida, contendo meio diluente sem glicerol (Botubov® - meio I, Botupharma, Botucatu, SP). O sêmen foi então avaliado quanto à motilidade, vigor, concentração, morfologia espermática, integridade de DNA, atividade mitocondrial, viabilidade, integridade e funcionalidade da membrana. Após a avaliação, o sêmen foi diluído em meio comercial contendo glicerol (Botubov® - meio II, Botupharma, Botucatu, SP), em uma concentração de 100x106 de espermatozoides/mL. Ato contínuo, as amostras foram envasadas em palhetas de 0,5 mL, refrigeradas a 4ºC, durante 4 horas, congeladas em caixa de poliestireno expandido, a 6cm do nível de nitrogênio líquido, durante 20 minutos e, posteriormente, armazenada em botijão criogênico. As amostras foram descongeladas a 37°C, durante 30 segundos. Após a descongelação, o sêmen foi reavaliado para os mesmos parâmetros, com exceção da concentração espermática e volume. Quanto à comparação dos resultados entre o sêmen fresco e congelado, estes foram superiores (p<0,05) no sêmen fresco quanto à motilidade (74,0 ± 7,0% versus 30,0 ± 8,2%), vigor (3,6 ± 0,7 versus 1,3 ± 0,5), integridade de membrana (fluorescência, 95,6 ± 3,1% versus 75,7 ± 5,9%) e índice de atividade citoquímica mitocondrial (99,3 ± 6,6 versus 40,0 ± 6,3), respectivamente. Concluiuse que a viabilidade do sêmen congelado obtido do epidídimo de touros é semelhante à do sêmen ejaculado, tornando a técnica de colheita aplicável comercialmente.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade de Franca (UNIFRAN), Franca, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Ciências Agrárias e Veterinárias (FCAV), Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Jaboticabal, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Faculdade de Ciências Agrárias e Veterinárias (FCAV), Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Jaboticabal, SP, BrasilUniversidade de Franca (UNIFRAN)Universidade Estadual Paulista (Unesp)Barbosa, Luciano MunitaKanazawa, Mábilis YumiPeres, Anelise Ribeiro [UNESP]Souza, Fabiana Ferreira de2016-07-07T12:36:25Z2016-07-07T12:36:25Z2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article190-194application/pdfhttp://dx.doi.org/10.4322/rbcv.2014.097Revista Brasileira de Ciência Veterinária, v. 19, n. 3, p. 190-194, 2012.1413-0130http://hdl.handle.net/11449/14101210.4322/rbcv.2014.097ISSN1413-0130-2012-19-03-190-194.pdf72945822894030497294582289403049Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporRevista Brasileira de Ciência Veterináriainfo:eu-repo/semantics/openAccess2024-06-06T18:09:46Zoai:repositorio.unesp.br:11449/141012Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:50:56.231199Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Viabilidade do sêmen congelado obtido do epidídimo de touros post-mortem
Viability of frozen semen obtained from epididymis of bulls post-mortem
title Viabilidade do sêmen congelado obtido do epidídimo de touros post-mortem
spellingShingle Viabilidade do sêmen congelado obtido do epidídimo de touros post-mortem
Barbosa, Luciano Munita
Criopreserved
Ejaculated
Bovine
Sperm
Epididymal
Criopreservação
Ejaculado
Bovino
Espermatozoide
Epididimal
title_short Viabilidade do sêmen congelado obtido do epidídimo de touros post-mortem
title_full Viabilidade do sêmen congelado obtido do epidídimo de touros post-mortem
title_fullStr Viabilidade do sêmen congelado obtido do epidídimo de touros post-mortem
title_full_unstemmed Viabilidade do sêmen congelado obtido do epidídimo de touros post-mortem
title_sort Viabilidade do sêmen congelado obtido do epidídimo de touros post-mortem
author Barbosa, Luciano Munita
author_facet Barbosa, Luciano Munita
Kanazawa, Mábilis Yumi
Peres, Anelise Ribeiro [UNESP]
Souza, Fabiana Ferreira de
author_role author
author2 Kanazawa, Mábilis Yumi
Peres, Anelise Ribeiro [UNESP]
Souza, Fabiana Ferreira de
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade de Franca (UNIFRAN)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Barbosa, Luciano Munita
Kanazawa, Mábilis Yumi
Peres, Anelise Ribeiro [UNESP]
Souza, Fabiana Ferreira de
dc.subject.por.fl_str_mv Criopreserved
Ejaculated
Bovine
Sperm
Epididymal
Criopreservação
Ejaculado
Bovino
Espermatozoide
Epididimal
topic Criopreserved
Ejaculated
Bovine
Sperm
Epididymal
Criopreservação
Ejaculado
Bovino
Espermatozoide
Epididimal
description The objective of this study was to evaluate the effects of freezing on the integrity and functionality of bovine epididymal spermatozoa collected postmortem. Ten pairs of testicle epididymis of bulls from a commercial slaughterhouse were used. On the laboratory, the testicle was separated and the semen was collected from the tail of epididymis on a petri dish heated containing a medium (Botubov ®, Botupharma, Botucatu, SP). Then, the semen was evaluated to motility, vigor, concentration, morphology, DNA integrity, mitochondrial activity, viability, membrane integrity and functionality. The semen was diluted in commercial extender (Botubov ®, Botupharma, Botucatu, SP) at a concentration of 100x106 sperm/mL. Thereafter, the samples were packaged in straws 0.5 mL, chilled at 4° C, for 4 hours, frozen in polystyrene box, at 6 cm from level of liquid nitrogen, during 20 minutes and stored in cryogenic container. The samples were thawed at 37°C for 30 seconds. After thawing, the semen was reevaluated to the same parameters, except sperm concentration and volume. Comparison of results between fresh and frozen semen showed means were increased (p<0.05) fresh semen motility (74,0 ± 7,0% versus 30,0 ± 8,2%), vigor (3,6 ± 0,7 versus 1,3 ± 0,5), membrane integrity (fluorescence, 95,6 ± 3,1% versus 75,7 ± 5,9%) and index of mitochondrial activity (99,3 ± 6,6 versus 40,0 ± 6,3), respectively. In conclusion, the viability of frozen semen obtained from the epididymis of bulls is similar than ejaculated semen, making the collection technique commercially applicable.
publishDate 2012
dc.date.none.fl_str_mv 2012
2016-07-07T12:36:25Z
2016-07-07T12:36:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.4322/rbcv.2014.097
Revista Brasileira de Ciência Veterinária, v. 19, n. 3, p. 190-194, 2012.
1413-0130
http://hdl.handle.net/11449/141012
10.4322/rbcv.2014.097
ISSN1413-0130-2012-19-03-190-194.pdf
7294582289403049
7294582289403049
url http://dx.doi.org/10.4322/rbcv.2014.097
http://hdl.handle.net/11449/141012
identifier_str_mv Revista Brasileira de Ciência Veterinária, v. 19, n. 3, p. 190-194, 2012.
1413-0130
10.4322/rbcv.2014.097
ISSN1413-0130-2012-19-03-190-194.pdf
7294582289403049
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Revista Brasileira de Ciência Veterinária
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 190-194
application/pdf
dc.source.none.fl_str_mv Currículo Lattes
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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