Expressão imunoistoquímica e molecular da laminina-332 cadeia gama-2 em tumores mamários de cadelas
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0102-09352011000100005 http://hdl.handle.net/11449/219641 |
Resumo: | Forty-eight cases of canine mammary cancer were investigated to evaluate the immunohistochemical distribution of the γ2 chain of laminin-332. Tumor cells were compared to a pool of normal mammary tissues using quantitative RT-PCR. The western blot was performed in eight tumor samples as complementary test to evaluate protein integrity. Immunohistochemistry experiments showed negative, focal, and weak expression of laminin-332 γ2 in tumors with the worst prognosis. Quantitative PCR revealed downregulation of the gene in 27 (56.2%) of the animals. Out of the 16 dogs with γ2 chain overexpression, seven were still alive. The western blot results showed bands generation of 36, 50, and 98kDa, suggesting degradation of laminin-332 γ2 in malignant tumors. The results suggest that, in the future, low expression and/or degradation of laminin-332 γ2 chain in canine mammary tumors may be used as an indicator of malignant potential. However, further studies are necessary to corroborate these results. |
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Expressão imunoistoquímica e molecular da laminina-332 cadeia gama-2 em tumores mamários de cadelasImmunohistochemical and molecular expression of laminin-332 gamma-2 chain in canine mammary tumorsAdhesion moleculeCancerDogRT-PCRWestern blotForty-eight cases of canine mammary cancer were investigated to evaluate the immunohistochemical distribution of the γ2 chain of laminin-332. Tumor cells were compared to a pool of normal mammary tissues using quantitative RT-PCR. The western blot was performed in eight tumor samples as complementary test to evaluate protein integrity. Immunohistochemistry experiments showed negative, focal, and weak expression of laminin-332 γ2 in tumors with the worst prognosis. Quantitative PCR revealed downregulation of the gene in 27 (56.2%) of the animals. Out of the 16 dogs with γ2 chain overexpression, seven were still alive. The western blot results showed bands generation of 36, 50, and 98kDa, suggesting degradation of laminin-332 γ2 in malignant tumors. The results suggest that, in the future, low expression and/or degradation of laminin-332 γ2 chain in canine mammary tumors may be used as an indicator of malignant potential. However, further studies are necessary to corroborate these results.Faculdade de Medicina de São José do Rio Preto, Av. Brigadeiro Faria Lima, 5416, 15090-000 - São José do Rio PretoIBILCE UNESP, São José do Rio Preto, SPIBILCE UNESP, São José do Rio Preto, SPFaculdade de Medicina de São José do Rio PretoUniversidade Estadual Paulista (UNESP)Zuccari, D. A.P.C.Castro, R.Jardim, B. V. [UNESP]Mancini, U. M.Polachini, G. M.2022-04-28T18:56:45Z2022-04-28T18:56:45Z2011-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article28-35http://dx.doi.org/10.1590/S0102-09352011000100005Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, v. 63, n. 1, p. 28-35, 2011.0102-09351678-4162http://hdl.handle.net/11449/21964110.1590/S0102-093520110001000052-s2.0-79955437232Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporArquivo Brasileiro de Medicina Veterinaria e Zootecniainfo:eu-repo/semantics/openAccess2022-04-28T18:56:45Zoai:repositorio.unesp.br:11449/219641Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T18:56:45Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Expressão imunoistoquímica e molecular da laminina-332 cadeia gama-2 em tumores mamários de cadelas Immunohistochemical and molecular expression of laminin-332 gamma-2 chain in canine mammary tumors |
title |
Expressão imunoistoquímica e molecular da laminina-332 cadeia gama-2 em tumores mamários de cadelas |
spellingShingle |
Expressão imunoistoquímica e molecular da laminina-332 cadeia gama-2 em tumores mamários de cadelas Zuccari, D. A.P.C. Adhesion molecule Cancer Dog RT-PCR Western blot |
title_short |
Expressão imunoistoquímica e molecular da laminina-332 cadeia gama-2 em tumores mamários de cadelas |
title_full |
Expressão imunoistoquímica e molecular da laminina-332 cadeia gama-2 em tumores mamários de cadelas |
title_fullStr |
Expressão imunoistoquímica e molecular da laminina-332 cadeia gama-2 em tumores mamários de cadelas |
title_full_unstemmed |
Expressão imunoistoquímica e molecular da laminina-332 cadeia gama-2 em tumores mamários de cadelas |
title_sort |
Expressão imunoistoquímica e molecular da laminina-332 cadeia gama-2 em tumores mamários de cadelas |
author |
Zuccari, D. A.P.C. |
author_facet |
Zuccari, D. A.P.C. Castro, R. Jardim, B. V. [UNESP] Mancini, U. M. Polachini, G. M. |
author_role |
author |
author2 |
Castro, R. Jardim, B. V. [UNESP] Mancini, U. M. Polachini, G. M. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Faculdade de Medicina de São José do Rio Preto Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Zuccari, D. A.P.C. Castro, R. Jardim, B. V. [UNESP] Mancini, U. M. Polachini, G. M. |
dc.subject.por.fl_str_mv |
Adhesion molecule Cancer Dog RT-PCR Western blot |
topic |
Adhesion molecule Cancer Dog RT-PCR Western blot |
description |
Forty-eight cases of canine mammary cancer were investigated to evaluate the immunohistochemical distribution of the γ2 chain of laminin-332. Tumor cells were compared to a pool of normal mammary tissues using quantitative RT-PCR. The western blot was performed in eight tumor samples as complementary test to evaluate protein integrity. Immunohistochemistry experiments showed negative, focal, and weak expression of laminin-332 γ2 in tumors with the worst prognosis. Quantitative PCR revealed downregulation of the gene in 27 (56.2%) of the animals. Out of the 16 dogs with γ2 chain overexpression, seven were still alive. The western blot results showed bands generation of 36, 50, and 98kDa, suggesting degradation of laminin-332 γ2 in malignant tumors. The results suggest that, in the future, low expression and/or degradation of laminin-332 γ2 chain in canine mammary tumors may be used as an indicator of malignant potential. However, further studies are necessary to corroborate these results. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-02-01 2022-04-28T18:56:45Z 2022-04-28T18:56:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0102-09352011000100005 Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, v. 63, n. 1, p. 28-35, 2011. 0102-0935 1678-4162 http://hdl.handle.net/11449/219641 10.1590/S0102-09352011000100005 2-s2.0-79955437232 |
url |
http://dx.doi.org/10.1590/S0102-09352011000100005 http://hdl.handle.net/11449/219641 |
identifier_str_mv |
Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, v. 63, n. 1, p. 28-35, 2011. 0102-0935 1678-4162 10.1590/S0102-09352011000100005 2-s2.0-79955437232 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Arquivo Brasileiro de Medicina Veterinaria e Zootecnia |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
28-35 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046765865205760 |